LOCUS       pEK36_Hsapiens_Hph-mCl 11636 bp ds-DNA     circular     01-OCT-2023
DEFINITION  .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     Imported using the Genbank importer. File name:
            135490-attachment_binDhx7DgcOVP0nDqA.gbk synthetic circular DNA
FEATURES             Location/Qualifiers
     misc_feature    1..25
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="note: right border repeat from nopaline C58 T-DNA"
     primer_bind     221..237
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="note: common sequencing primer, one of multiple similar variants"
     promoter        244..528
                     /label="SpH2Bpro"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             536..1561
                     /label="Hshph (HygR)"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="gene: aph(4)-Ia product: aminoglycoside phosphotransferase from E. coli note: confers resistance to hygromycin translation: MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             join(536..1561,1562..1576,1577..2290,2291..2296)
                     /label="Hshph-G4S-HsmClover3"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKEGGGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHYVYITADKQKNCIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK*"
     primer          1552..1571
                     /label="pEK36_Insert_1_fw"
                     /note="sequence: ggcaaaggaaggaggaggag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1562..1576
                     /label="G4S Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             1577..2296
                     /label="HsmClover3"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="product: bright green-yellow fluorescent protein derived from mClover2, with improved photostability (Bajar et al., 2003) note: mammalian codon-optimized translation: MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHYVYITADKQKNCIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK"
     primer          complement(2273..2322)
                     /label="pEK36_Insert_1_rv"
                     /note="sequence: attcgcttatttagaagtggcgcgccttacttgtacagctcgtccatgcc"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     terminator      2317..2504
                     /label="ScADH1ter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="gene: S. cerevisiae ADH1 note: transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2519..2535)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="note: common sequencing primer, one of multiple similar variants"
     protein_bind    2543..2559
                     /label="lac operator"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2567..2597)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="note: promoter for the E. coli lac operon"
     misc_feature    2810..2834
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="note: left border repeat from nopaline C58 T-DNA"
     CDS             3127..3942
                     /label="KanR"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="gene: aph(3')-Ia product: aminoglycoside phosphotransferase note: confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes translation: MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      4037..4625
                     /label="ori"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="direction: RIGHT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     rep_origin      5295..5489
                     /label="pVS1 oriV"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="note: origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)"
     CDS             complement(5555..6622)
                     /label="pVS1 RepA"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="product: replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000) translation: GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS"
     CDS             complement(7057..7686)
                     /label="pVS1 StaA"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="product: stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000) translation: MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSARDWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIPVQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPILESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     rep_origin      8853..10189
                     /label="2µ Ori"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        10451..10666
                     /label="URA3pro"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="gene: S. cerevisiae URA3"
     CDS             10667..11470
                     /label="URA3"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="gene: S. cerevisiae URA3 product: orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis note: yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA) translation: MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELLELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc tcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      121 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      181 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      241 aagcttttat gctccaagcg gagacgatga ggccgatatt acagtggctt agtagcgtgt
      301 acaatatttc cataaaaggc tacatggtga gtttttcacg tgaccgtaat tagtcccaat
      361 taggctttat tgtatattgc tactaagcat cgttaaatga cctgctcgaa gcttcgtcta
      421 taaaaggcgg gcgtgtgtgc gcttcccccg ctacgtcttt ttcacaaaca ctttgcgact
      481 tgaccaagac ctttcacccg caccactctc atccatcaca cacacaaatt aattaatggg
      541 taaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
      601 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt
      661 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg
      721 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg
      781 ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca
      841 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc
      901 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat
      961 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca
     1021 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct
     1081 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc
     1141 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
     1201 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg
     1261 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg
     1321 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg
     1381 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc
     1441 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
     1501 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga
     1561 aggaggagga ggatccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     1621 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtccgcg gcgagggcga
     1681 gggcgatgcc accaacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     1741 cgtgccctgg cccaccctcg tgaccacctt cggctacggc gtggcctgct tcagccgcta
     1801 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     1861 ggagcgcacc atctctttca aggacgacgg tacctacaag acccgcgccg aggtgaagtt
     1921 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     1981 caacatcctg gggcacaagc tggagtacaa cttcaacagc cactacgtct atatcacggc
     2041 cgacaagcag aagaactgca tcaaggctaa cttcaagatc cgccacaacg ttgaggacgg
     2101 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     2161 gctgcccgac aaccactacc tgagccatca gtccaagctg agcaaagacc ccaacgagaa
     2221 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggattacac atggcatgga
     2281 cgagctgtac aagtaaggcg cgccacttct aaataagcga atttcttatg atttatgatt
     2341 tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag
     2401 gttttaaaac gaaaattctt attcttgagt aactctttcc tgtaggtcag gttgctttct
     2461 caggtatagt atgaggtcgc tcttattgac cacacctcta ccgggaattc gtaatcatgt
     2521 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     2581 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     2641 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     2701 gccaacgcgc ggggagaggc ggtttgcgta ttggctagag caattcggcg ttaattcagt
     2761 acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac
     2821 aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac
     2881 cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc
     2941 ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt
     3001 tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc
     3061 tgcctgtgat ccagatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     3121 ggtgttatga gccatattca acgggaaacg tcttgctcta ggccgcgatt aaattccaac
     3181 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     3241 acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     3301 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     3361 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     3421 actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     3481 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     3541 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     3601 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     3661 tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     3721 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     3781 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     3841 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     3901 aataaattgc agtttcattt gatgctcgat gagtttttct aagaattaat tcatgatcct
     3961 gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     4021 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     4081 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     4141 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     4201 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     4261 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     4321 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     4381 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     4441 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4501 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4561 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4621 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4681 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     4741 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     4801 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     4861 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
     4921 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
     4981 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     5041 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt
     5101 gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc
     5161 ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg
     5221 cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc
     5281 gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag
     5341 agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc
     5401 ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct
     5461 ttgggttccc aatgtacgtg ctatccacag gaaagagacc ttttcgacct ttttcccctg
     5521 ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct
     5581 cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca
     5641 aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct
     5701 tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg
     5761 ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca
     5821 aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt
     5881 acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga
     5941 tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg
     6001 ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca
     6061 ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt
     6121 gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt
     6181 cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg
     6241 ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag
     6301 ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc
     6361 gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg
     6421 gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat
     6481 cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg
     6541 cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta
     6601 ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc
     6661 attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca
     6721 catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt
     6781 agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga
     6841 tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct
     6901 tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca
     6961 ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt
     7021 ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc
     7081 agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt
     7141 tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat
     7201 gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat
     7261 cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt
     7321 aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat
     7381 cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc
     7441 gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg
     7501 gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact
     7561 gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc
     7621 tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac
     7681 cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc
     7741 gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt
     7801 tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg
     7861 atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg
     7921 atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc
     7981 ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg
     8041 tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc
     8101 tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt
     8161 tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga
     8221 gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg
     8281 tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca
     8341 tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc
     8401 cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt
     8461 ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct
     8521 ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg
     8581 cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc
     8641 ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag
     8701 tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg
     8761 cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg
     8821 aacgtcagaa ggaaaagtgc cacctgaacg aagcatctgt gcttcatttt gtagaacaaa
     8881 aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac
     8941 agaaatgcaa cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa
     9001 acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac
     9061 agaacagaaa tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt
     9121 gttctacaaa aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt
     9181 ttttctcctt tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg
     9241 ttaaggttag aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact
     9301 ccacttcccg cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg
     9361 catccccgat tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat
     9421 agcgttgatg attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta
     9481 tatactacgt ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag
     9541 ttcttactac aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga
     9601 ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata
     9661 tagcacagag atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg
     9721 caatatttta gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc
     9781 agagcgcttt tggttttcaa aagcgctctg aagttcctat actttctaga gaataggaac
     9841 ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag
     9901 ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat
     9961 atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc
    10021 tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg
    10081 ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa
    10141 ttggattagt ctcatccttc aatgctatca tttcctttga tattggatca tactaagaaa
    10201 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
    10261 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
    10321 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
    10381 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
    10441 ataccacctt ttcaattcat catttttttt ttattctttt ttttgatttc ggtttccttg
    10501 aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg agcacagact
    10561 tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc cagtattctt
    10621 aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt cgaaagctac
    10681 atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat ttaatatcat
    10741 gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca aggaattact
    10801 ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg tggatatctt
    10861 gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa
    10921 ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca aattgcagta
    10981 ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt
    11041 gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa aggaacctag
    11101 aggccttttg atgctagcag aattgtcatg caagggctcc ctatctactg gagaatatac
    11161 taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca
    11221 aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg
    11281 tttagatgac aagggagacg cattgggtca acagtataga accgtggatg atgtggtctc
    11341 tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa gggatgctaa
    11401 ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa gatgcggcca
    11461 gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac aaattagagc
    11521 ttcaatttaa ttatatcagt tattacctgg catgcacata caaatggacg aacggataaa
    11581 ccttttcacg cccttttaaa tatccgatta ttctaataaa cgctcttttc tcttag
//