LOCUS       Exported                1805 bp ds-DNA     circular SYN 16-OCT-2023
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Oct 16, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source synthetic DNA construct
     rep_origin      17..605
                     /locus_tag="colE1"
                     /label=colE1
     misc_feature    164
                     /label=T to C reduces copy number
     promoter        633..651
                     /locus_tag="T7\prom"
                     /label=T7 prom
                     /label=T7\prom
                     /note="/vntifkey=30"
     RBS             683..705
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(692..739)
                     /label=SDM-1.R
     CDS             713..754
                     /codon_start=1
                     /locus_tag="sfGFP"
                     /label=FETCH1
                     /label=sfGFP
                     /note="/vntifkey=21"
                     /translation="MFLNCCPGCCMEP"
     CDS             725..742
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     primer_bind     728..764
                     /label=SDM-1.F
     terminator      820..867
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    complement(885..1700)
                     /locus_tag="kan2\marker"
                     /label=kan2 marker
                     /label=kan2\marker
                     /label=nonstandard type: Marker
                     /note="/vntifkey=21"
                     /note="/codon_start=1"
                     /note="/translation=MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGAT
                     IYRLY 
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP 
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL 
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG 
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
       61 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      601 ggaaaaacgc cagcaacgcg atcccgcgaa attaatacga ctcactatag ggagaccaca
      661 acggtttccc tctagaaata attttgttta actttaagaa ggagatatac atatgttttt
      721 aaactgctgt cctgggtgtt gtatggaacc gtaagtcgac cggctgctaa caaagcccga
      781 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
      841 tctaaacggg tcttgagggg ttttttgctg aaagccaatt ctgattagaa aaactcatcg
      901 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa
      961 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc
     1021 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg
     1081 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat
     1141 ggcaaaagct tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca
     1201 tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga
     1261 aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg
     1321 aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg
     1381 aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata
     1441 aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca
     1501 tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg
     1561 ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat
     1621 ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcttcga gcaagacgtt
     1681 tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt
     1741 attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca
     1801 acgtg
//
