LOCUS       Exported                1829 bp ds-DNA     circular SYN 16-OCT-2023
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Oct 16, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source synthetic DNA construct
     source          752..775
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source synthetic DNA construct
     rep_origin      17..605
                     /locus_tag="colE1"
                     /label=colE1
     misc_feature    164
                     /label=T to C reduces copy number
     promoter        633..651
                     /locus_tag="T7\prom"
                     /label=T7 prom
                     /label=T7\prom
                     /note="/vntifkey=30"
     RBS             683..705
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             713..778
                     /codon_start=1
                     /locus_tag="sfGFP"
                     /label=FETCH1-StrepII
                     /label=sfGFP
                     /note="/vntifkey=21"
                     /translation="MFLNCCPGCCMEPWSHPQFEK"
     CDS             725..742
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             752..775
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an
                     engineered form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     terminator      844..891
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    complement(909..1724)
                     /locus_tag="kan2\marker"
                     /label=kan2 marker
                     /label=kan2\marker
                     /label=nonstandard type: Marker
                     /note="/vntifkey=21"
                     /note="/codon_start=1"
                     /note="/translation=MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGAT
                     IYRLY 
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP 
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL 
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG 
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
       61 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      601 ggaaaaacgc cagcaacgcg atcccgcgaa attaatacga ctcactatag ggagaccaca
      661 acggtttccc tctagaaata attttgttta actttaagaa ggagatatac atatgttttt
      721 aaactgctgt cctgggtgtt gtatggaacc gtggagccat ccgcagttcg aaaaataagt
      781 cgaccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
      841 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagcc
      901 aattctgatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga
      961 ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg
     1021 cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca
     1081 atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga
     1141 gtgacgactg aatccggtga gaatggcaaa agcttatgca tttctttcca gacttgttca
     1201 acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt
     1261 cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca
     1321 ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa
     1381 tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac
     1441 catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc
     1501 agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt
     1561 ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat
     1621 tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt
     1681 aatcgcggct tcgagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta
     1741 ctgtttatgt aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg
     1801 taacatcaga gattttgaga cacaacgtg
//
