LOCUS       Exported                2039 bp ds-DNA     circular SYN 16-OCT-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Oct 16, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     >gi|37575398|gb|AY327136.1|RibosomedisplayvectorpRDV,completesequen 
            ce
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          345..380
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source synthetic DNA construct
     source          381..383
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source synthetic DNA construct
     source          join(384..2039,1..107)
                     /organism="Tth_rplA_L1 in pJL1"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    45..65
                     /label=5'stem loop
     RBS             78..100
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             108..383
                     /codon_start=1
                     /label=sFLAG-p5-PRB4_R7-9-H1
                     /translation="MDYKDEDLLPPPPPIPKPEGRPPQGGNQSQGPPPHPGKPERPPPQ
                     GGNQSHRPPPPPGKPERPPPQGGNQSQGPPPHPGFLNCCPGCCMEP"
     misc_feature    111..134
                     /label=superFLAG
                     /note="superFLAG variant (DYKDEDLL) derived from 
                     https://doi.org/10.1016/j.molcel.2019.02.019 higher binding
                     to M2 anti-FLAG antibody than either FLAG or 2x/3x FLAG"
     misc_feature    156..344
                     /label=R7-R8-R9 of PRB4
     CDS             345..380
                     /codon_start=1
                     /label=FETCH1
                     /translation="FLNCCPGCCMEP"
     terminator      449..496
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(488..513)
                     /label=T7_term.rev
     CDS             complement(514..1329)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(726..751)
                     /label=pJL1_LET.rev
     rep_origin      1451..2039
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1821..1842
                     /label=pJL1_LET.fwd
ORIGIN
        1 aacgccagca acgcgatccc gcgaaattaa tacgactcac tatagggaga ccacaacggt
       61 ttccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg gactacaaag
      121 acgaagacct gctgccgccc cctcctccta tcccaaagcc tgaaggacga ccccctcagg
      181 gtgggaacca atcgcaggga ccacccccgc atcccggaaa accagagaga ccaccgcctc
      241 aaggtggcaa tcagtctcac agaccacccc caccacccgg aaaacctgaa agacccccac
      301 ctcaaggcgg taaccaatcc caaggtccgc cccctcatcc gggcttttta aactgctgtc
      361 ctgggtgttg tatggaaccg taagtcgacc ggctgctaac aaagcccgaa aggaagctga
      421 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
      481 cttgaggggt tttttgctga aagccaattc tgattagaaa aactcatcga gcatcaaatg
      541 aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg
      601 taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc
      661 tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag
      721 gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagctt
      781 atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact
      841 cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc
      901 gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag
      961 cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt
     1021 cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat
     1081 ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc
     1141 attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata
     1201 caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata
     1261 taaatcagca tccatgttgg aatttaatcg cggcttcgag caagacgttt cccgttgaat
     1321 atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga
     1381 tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa cgtgagatca
     1441 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     1501 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     1561 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     1621 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     1681 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     1741 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1801 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     1861 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     1921 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     1981 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaa
//
