LOCUS       P75_-_pTT3-ecdCD86-ST3- 6761 bp ds-DNA     circular     13-SEP-2023
DEFINITION  .
KEYWORDS    "creator:Johannes Pettmann" "marker:AmpR"
FEATURES             Location/Qualifiers
     enhancer        154..533
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: human cytomegalovirus (CMV) immediate early promoter"
     misc_feature    1302..1311
                     /label="Kozak sequence"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: vertebrate consensus sequence for strong initiation of translation (Kozak, 1987) regulatory_class: other: "
     CDS             1308..1391
                     /label="chicken RPTPσ signal peptide"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="codon_start: 1 transl_table: 1 translation: MGILPSPGMPALLSLVSLLSVLLMGCVA"
     CDS             1392..1400
                     /label="Age1 link"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 transl_table: 1 translation: ETG"
     CDS             1401..2045
                     /label="ECD"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 transl_table: 1 translation: APLKIQAYFNETADLPCQFANSQNQSLSELVVFWQDQENLVLNEVYLGKEKFDSVHSKYMGRTSFDSDSWTLRLHNLQIKDKGLYQCIIHHKKPTGMIRIHQMNSELSVLANFSQPEIVPISNITENVYINLTCSSIHGYPEPKKMSVLLRTKNSTIEYDGIMQKSQDNVTELYDVSISLSVSFPDVTSNMTIFCILETDKTRLLSSPFSIELED"
     misc_structure  1428..1724
                     /label="Ig-like V-type"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_structure  1779..2006
                     /label="Ig-like C2-type"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     variation       1884..1886
                     /label="V->I polymorphism"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="product: In ~10% of population."
     CDS             2046..2093
                     /label="SpyTAGv3"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
                     /note="codon_start: 1 transl_table: 1 translation: RGVPHIVMVDAYKRYK"
     misc_feature    2055..2084
                     /label="30 bp homology"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             2094..2111
                     /label="6xHis"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 product: 6xHis affinity tag transl_table: 1 translation: HHHHHH"
     polyA_site      2141..2196
                     /label="β-globin poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: rabbit β-globin polyadenylation signal"
     promoter        4518..4622
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: bla"
     CDS             4623..4691
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     CDS             4692..5483
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     rep_origin      5654..6242
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     protein_bind    6530..6551
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: E. coli catabolite activator protein note: CAP binding activates transcription in the presence of cAMP."
     promoter        6566..6571
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        6572..6589
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        6590..6596
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     protein_bind    6604..6620
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     6628..6644
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt aaacggatct ctagcgaatt cGCCACCATG GGGATACTTC
     1321 CCAGCCCTGG GATGCCTGCG CTGCTCTCCC TCGTGAGCCT TCTCTCCGTG CTGCTGATGG
     1381 GTTGCGTAGC TGAAACCGGT GCTCCTCTGA AGATTCAAGC TTATTTCAAT GAGACTGCAG
     1441 ACCTGCCATG CCAATTTGCA AACTCTCAAA ACCAAAGCCT GAGTGAGCTA GTAGTATTTT
     1501 GGCAGGACCA GGAAAACTTG GTTCTGAATG AGGTATACTT AGGCAAAGAG AAATTTGACA
     1561 GTGTTCATTC CAAGTATATG GGCCGCACAA GTTTTGATTC GGACAGTTGG ACCCTGAGAC
     1621 TTCACAATCT TCAGATCAAG GACAAGGGCT TGTATCAATG TATCATCCAT CACAAAAAGC
     1681 CCACAGGAAT GATTCGCATC CACCAGATGA ATTCTGAACT GTCAGTGCTT GCTAACTTCA
     1741 GTCAACCTGA AATAGTACCA ATTTCTAATA TAACAGAAAA TGTGTACATA AATTTGACCT
     1801 GCTCATCTAT ACACGGTTAC CCAGAACCTA AGAAGATGAG TGTTTTGCTA AGAACCAAGA
     1861 ATTCAACTAT CGAGTATGAT GGTATTATGC AGAAATCTCA AGATAATGTC ACAGAACTGT
     1921 ACGACGTTTC CATCAGCTTG TCTGTTTCAT TCCCTGATGT TACGAGCAAT ATGACCATCT
     1981 TCTGTATTCT GGAAACTGAC AAGACGCGGC TTTTATCTTC ACCTTTCTCT ATAGAGCTTG
     2041 AGGACAGAGG CGTCCCTCAT ATCGTGATGG TCGACGCCTA TAAGAGGTAT AAACATCATC
     2101 ACCATCACCA TTAAggatcc cccgacctcg acctctggct aataaaggaa atttattttc
     2161 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
     2221 aatcatttgg tcgagatccc tcggagatct ctagctagag gatcgatccc cgccccggac
     2281 gaactaaacc tgactacgac atctctgccc cttcttcgcg gggcagtgca tgtaatccct
     2341 tcagttggtt ggtacaactt gccaactggg ccctgttcca catgtgacac ggggggggac
     2401 caaacacaaa ggggttctct gactgtagtt gacatcctta taaatggatg tgcacatttg
     2461 ccaacactga gtggctttca tcctggagca gactttgcag tctgtggact gcaacacaac
     2521 attgccttta tgtgtaactc ttggctgaag ctcttacacc aatgctgggg gacatgtacc
     2581 tcccaggggc ccaggaagac tacgggaggc tacaccaacg tcaatcagag gggcctgtgt
     2641 agctaccgat aagcggaccc tcaagagggc attagcaata gtgtttataa ggcccccttg
     2701 ttaaccctaa acgggtagca tatgcttccc gggtagtagt atatactatc cagactaacc
     2761 ctaattcaat agcatatgtt acccaacggg aagcatatgc tatcgaatta gggttagtaa
     2821 aagggtccta aggaacagcg atatctccca ccccatgagc tgtcacggtt ttatttacat
     2881 ggggtcagga ttccacgagg gtagtgaacc attttagtca caagggcagt ggctgaagat
     2941 caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct tcttcattct ccttcgttta
     3001 gctaatagaa taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa
     3061 ggtttcaggt gacgccccca gaataaaatt tggacggggg gttcagtggt ggcattgtgc
     3121 tatgacacca atataaccct cacaaacccc ttgggcaata aatactagtg taggaatgaa
     3181 acattctgaa tatctttaac aatagaaatc catggggtgg ggacaagccg taaagactgg
     3241 atgtccatct cacacgaatt tatggctatg ggcaacacat aatcctagtg caatatgata
     3301 ctggggttat taagatgtgt cccaggcagg gaccaagaca ggtgaaccat gttgttacac
     3361 tctatttgta acaaggggaa agagagtgga cgccgacagc agcggactcc actggttgtc
     3421 tctaacaccc ccgaaaatta aacggggctc cacgccaatg gggcccataa acaaagacaa
     3481 gtggccactc ttttttttga aattgtggag tgggggcacg cgtcagcccc cacacgccgc
     3541 cctgcggttt tggactgtaa aataagggtg taataacttg gctgattgta accccgctaa
     3601 ccactgcggt caaaccactt gcccacaaaa ccactaatgg caccccgggg aatacctgca
     3661 taagtaggtg ggcgggccaa gataggggcg cgattgctgc gatctggagg acaaattaca
     3721 cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc tcatattcac gaggtcgctg
     3781 agagcacggt gggctaatgt tgccatgggt agcatatact acccaaatat ctggatagca
     3841 tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca
     3901 tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca
     3961 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     4021 tatgctatcc taatctgtat ccgggtagca tatgctatcc taatagagat tagggtagta
     4081 tatgctatcc taatttatat ctgggtagca tatactaccc aaatatctgg atagcatatg
     4141 ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagcatagg
     4201 ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg
     4261 ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg
     4321 ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg
     4381 ctatcctcat gataagctgt caaacatgag aattttcttg aagacgaaag ggcctcgtga
     4441 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     4501 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     4561 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     4621 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     4681 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     4741 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     4801 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     4861 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     4921 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     4981 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     5041 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     5101 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     5161 tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     5221 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     5281 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     5341 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     5401 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     5461 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     5521 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     5581 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     5641 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5701 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5761 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     5821 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5881 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5941 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6001 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6061 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6121 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6181 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     6241 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6301 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     6361 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     6421 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     6481 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     6541 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     6601 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     6661 tctagctaga ggtcgaccaa ttctcatgtt tgacagctta tcatcgcaga tccgggcaac
     6721 gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg a
//