LOCUS       Exported                7581 bp ds-DNA     circular SYN 20-OCT-2023
DEFINITION  synthetic circular DNA
ACCESSION   <unknown
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Oct 24, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7581
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     repeat_region   651..780
                     /label=AAV2 ITR (alternate)
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=repeat region"
     misc_feature    complement(1337..1366)
                     /label=MCS
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=28.30188679245283"
                     /note="/other=misc feature"
     intron          1500..1632
                     /label=chimeric intron
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=99.254"
                     /note="/match_length=100.0"
                     /note="/other=intron"
     CDS             1745..3172
                     /codon_start=1
                     /label=tdTomato
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             3182..3238
                     /codon_start=1
                     /label=P2A
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3245..3961
                     /codon_start=1
                     /label=EGFP
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3977..4318
                     /codon_start=1
                     /label=Syb2
                     /translation="ATAATVPPAAPAGEGGPPAPPPNLTSNRRLQQTQAQVDEVVDIMR
                     VNVDKVLERDQKLSELDDRADALQAGASQFETSAAKLKRKYWWKNLKMMIILGVICAII
                     LIIIIVYFST"
     misc_feature    4334..4358
                     /label=MCS
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=23.58490566037736"
                     /note="/other=misc feature"
     misc_feature    4358..4946
                     /label=WPRE
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc feature"
     polyA_signal    4978..5454
                     /label=hGH poly(A) signal
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA signal"
     repeat_region   5494..5634
                     /label=AAV2 ITR
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=repeat region"
     misc_feature    complement(5639..5690)
                     /label=bom
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=37.410071942446045"
                     /note="/other=misc feature"
     rep_origin      5709..6164
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     misc_feature    complement(6165..6214)
                     /label=bom
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=35.97122302158273"
                     /note="/other=misc feature"
     CDS             complement(6316..6351)
                     /codon_start=1
                     /label=rop
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=19.047619047619047"
                     /note="/other=CDS"
                     /translation="LARFGDDGENL"
     promoter        6446..6550
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             6551..7411
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt cctgcaggca
      661 gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt ggtcgcccgg
      721 cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact aggggttcct
      781 gcggccgcac gcgtttattt cagaatgtta tgcactcaca gtggtttggc atgcatctgg
      841 tgaatttttt ttaacgaaaa attagtgttg gtttcgatgt atggtagcat tctccctaac
      901 gtaatttgaa taattcagca aagccccact accagctgta cttctgcagc ctcttccatt
      961 cttttcagca ttataatttt ggttaatttt caattttagg tcctacgtct ctgcaatttg
     1021 tgtatgaata acagaataat ttccctcttt tgtttcgcct ttcctgttcc tgaatctaaa
     1081 taaagatggc tttttagtat taaaagtgga agaaaattac aggtaattat ctttgacggt
     1141 aaaaacgctg taatcagcgg gctacatgaa aaattactct aattatggct gcatttaaga
     1201 gaatggaaaa aaaccttctt gtggataaaa accttaaatt gtccccaatg tctgcttcaa
     1261 attggatggc actgcagctg gaggctttgt tcagaattga tcctggggag ctacgaaccc
     1321 aaagtttcac agtaggaagc ttgatatcga attcctgcag cccgggctgg gcataaaagt
     1381 cagggcagag ccatctattg cttacatttg cttctagcct gcaggtcgag gagcgcagcc
     1441 ttccagaagc agagcgcggc gccttaagct gcagaagttg gtcgtgaggc actgggcagg
     1501 taagtatcaa ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac
     1561 agagaagact cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct
     1621 ttctctccac aggtgtccac tcccagttca attacagctc ttaagaaaga ccggtgccac
     1681 catgctgtgc tgtatgagaa gaaccaaaca ggttgaaaag aatgatgagg accaaaagat
     1741 cgagatggtg agcaagggcg aggaggtcat caaagagttc atgcgcttca aggtgcgcat
     1801 ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     1861 cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg
     1921 ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc accccgccga
     1981 catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     2041 cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct
     2101 gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg taatgcagaa
     2161 gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa
     2221 gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa
     2281 gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg tggacaccaa
     2341 gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga
     2401 gggccgccac cacctgttcc tggggcatgg caccggcagc accggcagcg gcagctccgg
     2461 caccgcctcc tccgaggaca acaacatggc cgtcatcaaa gagttcatgc gcttcaaggt
     2521 gcgcatggag ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg
     2581 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt
     2641 cgcctgggac atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc
     2701 cgccgacatc cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     2761 gatgaacttc gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg
     2821 cacgctgatc tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat
     2881 gcagaagaag accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt
     2941 gctgaagggc gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga
     3001 gttcaagacc atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga
     3061 caccaagctg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg
     3121 ctccgagggc cgccaccacc tgttcctgta cggcatggac gagctgtaca agggaagcgg
     3181 agctactaac ttcagcctgc tgaagcaggc tggagacgtg gaggagaacc ctggacctca
     3241 attcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     3301 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     3361 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     3421 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     3481 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     3541 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     3601 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     3661 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     3721 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     3781 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     3841 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     3901 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     3961 gggtggaggt ggatcggcta ccgctgccac cgtcccgcct gccgccccgg ccggcgaggg
     4021 tggcccccct gcacctcctc caaatcttac cagtaacagg agactgcagc agacccaggc
     4081 ccaggtggat gaggtggtgg acatcatgag ggtgaatgtg gacaaggtcc tggagcgaga
     4141 ccagaagcta tcggaactgg atgatcgcgc agatgccctc caggcagggg cctcccagtt
     4201 tgaaacaagt gcagccaagc tcaagcgcaa atactggtgg aaaaacctca agatgatgat
     4261 catcttggga gtgatttgcg ccatcatcct catcatcatc atcgtttact tcagcactta
     4321 agaattgaga tctgaattcg atatcaagct tatcgataat caacctctgg attacaaaat
     4381 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     4441 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     4501 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     4561 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     4621 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     4681 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     4741 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tatgttgcca cctggattct
     4801 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     4861 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     4921 gatctccctt tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga gatctacggg
     4981 tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg
     5041 cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc
     5101 tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc
     5161 tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc
     5221 actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg
     5281 ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt
     5341 ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg
     5401 cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt
     5461 ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca
     5521 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
     5581 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc
     5641 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
     5701 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     5761 cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt
     5821 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     5881 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
     5941 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     6001 taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt
     6061 tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca
     6121 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
     6181 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     6241 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     6301 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     6361 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     6421 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     6481 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     6541 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     6601 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     6661 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     6721 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     6781 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     6841 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     6901 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     6961 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     7021 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     7081 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     7141 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     7201 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     7261 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     7321 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     7381 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     7441 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     7501 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     7561 agaaaagatc aaaggatctt c
//
