LOCUS       L0-12_pL0M_PU_TOsTUb1A1_R        2892 bp    DNA     circular     10-NOV-2020
DEFINITION  OsTub1A1terminator_reverse orientation.
ACCESSION   urn.local.dlee.v-9o8y1nz
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI http://www.invitrogen.com/      
                                              COMMENT     ORIGDB|GenBank       
                                              COMMENT     VNTDATE|512379305|   
                                              COMMENT     VNTDBDATE|512379305| 
                                              COMMENT     LSOWNER|             
                                              COMMENT     VNTNAME|pICH41295|   
                                              COMMENT     VNTAUTHORNAME|Carola
            Engler|                            COMMENT    
            VNTAUTHOREML|engler@icongenetics.de|                    COMMENT    
            Vector_NTI_Display_Data_(Do_Not_Edit!)                  COMMENT    
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            #4=(CRSite (CStringList) "Esp3I" "cgtctc" 8 12 0 0 "") COMMENT     
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            #8=(CFSignal (CObList) "rep (pMB1)" 21 0 0 1385 1999 0
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FEATURES             Location/Qualifiers
     CDS             215..1225
                     /dnas_title="Sm/Sp no DraIII"
                     /vntifkey=4
                     /label="Sm/Sp no DraIII"
     mutation        1192
                     /dnas_title="G to A"
                     /vntifkey=62
                     /label="G to A"
     misc_feature    1385..1999
                     /dnas_title="rep (pMB1)"
                     /vntifkey=21
                     /label="rep (pMB1)"
     rep_origin      1984
                     /dnas_title="ORI"
                     /vntifkey=33
                     /note="RNaseH cleavage point"
                     /label="ORI"
     misc_feature    2232..2235
                     /dnas_title="GGAG"
                     /vntifkey=21
                     /label="GGAG"
     terminator      complement(2236..2876)
                     /dnas_title="OsTublinA1 Terminator"
                     /label="Tub1AT"
     misc_feature    complement(2314..2333)
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.9"
                     /Sequence="ATTGTGCGTTCCAGGTGAGT"
                     /created_by="primer3"
                     /annotation_group="225105221063369924980ea5-7ee2-49c5-8666
                     -6574d2b08c4burn:sequence:genbank:null"
                     /label="2,333 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    2877..2880
                     /dnas_title="AATG"
                     /vntifkey=21
                     /label="AATG"
ORIGIN      
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
      361 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
      421 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
      481 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
      541 cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt
      601 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
      661 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
      721 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
      781 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
      841 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
      901 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
      961 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     1021 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     1081 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     1141 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga
     1201 gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat
     1261 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1321 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1381 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1441 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1501 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1561 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1621 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1681 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1741 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1801 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1861 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1921 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1981 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2041 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2101 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2161 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact
     2221 ctgtggtctc aggagatagg ctagtcatgg tgactgcacg ttgtaagtgc agcaaactgc
     2281 cgacgcgatg caaactgtac acgttaacat gccactcacc tggaacgcac aatggccact
     2341 aggtgcggcc gtagtgtgga tttcaaagag agagagagag agagagagct aatcacgtaa
     2401 acgtaaacac agcagatagc agagatgttg attaggcaaa acagtataaa agccaatcca
     2461 ataaactaca tttagcgaag tgctatacta atgcactaat aacgaactgt tcttttctta
     2521 agatcggagc cagtaatggg ttgtcagcag gagaagcacg taaaccttga aacatactaa
     2581 gttccacagt cgagagtaaa ccgtaatcaa cacaagaaac aaacataaaa ttgaacaaac
     2641 gcgcatatta taagtgacga agcggtgtca cataaaacag ggcacacagg ttacaacaac
     2701 gagggttgta agcccattaa gccccaaaca tcagatcacc acaagcaaat gtctcgaaca
     2761 cacacgcaca cggcaacagg ataactccac actggcagat catgggatag cagcagttat
     2821 caatcaggcc ttgacacaca gaacatcgag cccccagacg acgacgactc ctctagaatg
     2881 tgagaccacg aa
//
