LOCUS       L0-33_pL0M-PU-pZmEF1a-15535        3666 bp    DNA     circular     08-OCT-2020
DEFINITION  pL0M-PU-pZmEF1a-15535.
ACCESSION   urn.local.dlee.c9-9qpj8kb
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     CDS             215..1225
                     /label="Sm/Sp no DraIII"
     mutation        1192
                     /label="G to A"
     misc_feature    1385..1999
                     /label="rep (pMB1)"
     rep_origin      complement(1984)
                     /label="ORI"
     misc_feature    2232..2235
                     /label="GGAG Fusion Site"
     misc_feature    2236..3650
                     /label="pZmEF1a"
                     /note="Geneious type: promoter uukaryotic"
     misc_feature    complement(2303..2322)
                     /Sequence="ATGTGATGAAGGATGGCGCA"
                     /Hairpin_Tm="None"
                     /Tm="60.1"
                     /Self_Dimer_Tm="10.8"
                     /%GC="50.0"
                     /modified_by="dlee"
                     /label="ZmEF1A_87R"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     3579..3598
                     /Sequence="TTTGCCTGACTGCGTGCTAT"
                     /Hairpin_Tm="None"
                     /Tm="60.3"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /modified_by="dlee"
                     /label="pZmEF1a_1344 F"
     misc_feature    3651..3654
                     /label="AATG Fusion Site"
ORIGIN      
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
      361 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
      421 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
      481 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
      541 cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt
      601 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
      661 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
      721 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
      781 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
      841 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
      901 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
      961 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     1021 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     1081 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     1141 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga
     1201 gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat
     1261 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1321 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1381 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1441 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1501 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1561 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1621 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1681 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1741 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1801 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1861 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1921 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1981 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2041 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2101 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2161 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact
     2221 ctgtggtctc aggagccaca accgaaaagt ccagacagag atcggttctg acaagcagaa
     2281 tcccgctagc atcgtagcaa agtgcgccat ccttcatcac atgttttttt gttcttctga
     2341 tatgagaaaa aggaaaggaa gcgccgtttt ttcgaaagaa aagaaaacga taaggcacgg
     2401 agaacgagtg accggcgcgt gaagcagcgg tggtggagtg ggcctagggc agggtagccg
     2461 tcaaggcagg gcgttcgtag gcgctacgaa agctcgagga ctgtactcgc tcgcaaaccc
     2521 tagccgccgg accggccggt gaccgtgcgg cgctgaggcg cgcgctatat aaacagagcc
     2581 atccattcct cccttaccgt tcagtcccac ctcgaccgcc tcctctcccc tcccccggct
     2641 ctgtctgttg acttaccccg tcgaggtaag gcactgggtc cttgaattca cggttttgat
     2701 tcagttgtta ctattcagtt ctgtctgaat tgtttgatgc tcgattcatt attaggcttt
     2761 caagttctgt aaattgttcc ctagtttttg tccttcacgc atatattact aggagtgttg
     2821 ctcttatgtc gcgcacctag ctaagctacc aatcgttttt ctttcttcca cttttataat
     2881 aaagcagata taccaaatca aatagttata cagtatatat catcgcatcg cccacctgat
     2941 taaattcaaa ttcaactaaa attggtgctc ccatcttcta acggtgaaaa cagagggtct
     3001 gatctcctat cctctcgggt gaggccgtgg gaccgggcct tgtggtatac tgtagttgat
     3061 ataattctga tataggttcg aatcctattg ggtgcttcaa tttagtttcg gcatctcctg
     3121 tttaattcgc tccactgcca gactcactca catagcctgc taggcaatat ctgtctgtgt
     3181 attcttggtt tagatacatc atgtttgctt gcctacttat atttattagc atggcatctt
     3241 gtatagcttg gttaattagt atattatatt atttgttcct gctgcatcta tctttatgta
     3301 ttcattcggc agttgtgttg aatgctctga tttgtcttgc tcggttgctt ttcattggta
     3361 tgaattacta gttacagatg ggtgaacact gactgcacgg tcactgtttt cttgatgact
     3421 gactatgttg tctttgctat tctaaacaaa agaacaatta atctaccagt ctgcttacgt
     3481 acatatgtgt ttctaatttg atgttagtca atgaatcgcc gcaactgttc taaaaaaaat
     3541 accaacctct caactgctaa ttgtagtata tcttaacttt tgcctgactg cgtgctatat
     3601 atatcctttt ttttgtgaat acagctttct agtcagctgc tcccgttgcc aatgtgagac
     3661 cacgaa
//
