LOCUS       pCMV_Flag-Halo-D       16963 bp DNA     circular SYN 27-OCT-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 27, 2023 from SnapGene Viewer 7.0.2
            https://www.snapgene.com
COMMENT     Created: Thursday, April 26, 2012 10:36 AM
FEATURES             Location/Qualifiers
     source          1..16963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        106..210
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             211..1071
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1:  211 ..  279 / #ccffcc / signal sequence
                        2:  280 .. 1071 / #ccffcc
                     Cleavage site after base 279"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1242..1830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     2087..2103
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     protein_bind    2137..2170
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
                     /note="color: #31849b; direction: RIGHT"
     enhancer        2314..2617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        2623..2900
                     /label=chicken beta-actin promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             3049..16494
                     /codon_start=1
                     /label=Flag-Halo-DNA-PKcs
                     /note="color: #993366"
                     /translation="MDYKDHDGDYKDHDIDYKDDDDKITSYNVCYTKLSGTMAEIGTGF
                     PFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLI
                     GMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGI
                     AFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVE
                     MDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPG
                     VLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGEPTTED
                     LYFQSDNAIASGTMAGSGAGVRCSLLRLQETLSAADRCGAALAGHQLIRGLGQECVLSS
                     SPAVLALQTSLVFSRDFGLLVFVRKSLNSIEFRECREEILKFLCIFLEKMGQKIAPYSV
                     EIKNTCTSVYTKDRAAKCKIPALDLLIKLLQTFRSSRLMDEFKIGELFSKFYGELALKK
                     KIPDTVLEKVYELLGLLGEVHPSEMINNAENLFRAFLGELKTQMTSAVREPKLPVLAGC
                     LKGLSSLLCNFTKSMEEDPQTSREIFNFVLKAIRPQIDLKRYAVPSAGLRLFALHASQF
                     STCLLDNYVSLFEVLLKWCAHTNVELKKAALSALESFLKQVSNMVAKNAEMHKNKLQYF
                     MEQFYGIIRNVDSNNKELSIAIRGYGLFAGPCKVINAKDVDFMYVELIQRCKQMFLTQT
                     DTGDDRVYQMPSFLQSVASVLLYLDTVPEVYTPVLEHLVVMQIDSFPQYSPKMQLVCCR
                     AIVKVFLALAAKGPVLRNCISTVVHQGLIRICSKPVVLPKGPESESEDHRASGEVRTGK
                     WKVPTYKDYVDLFRHLLSSDQMMDSILADEAFFSVNSSSESLNHLLYDEFVKSVLKIVE
                     KLDLTLEIQTVGEQENGDEAPGVWMIPTSDPAANLHPAKPKDFSAFINLVEFCREILPE
                     KQAEFFEPWVYSFSYELILQSTRLPLISGFYKLLSITVRNAKKIKYFEGVSPKSLKHSP
                     EDPEKYSCFALFVKFGKEVAVKMKQYKDELLASCLTFLLSLPHNIIELDVRAYVPALQM
                     AFKLGLSYTPLAEVGLNALEEWSIYIDRHVMQPYYKDILPCLDGYLKTSALSDETKNNW
                     EVSALSRAAQKGFNKVVLKHLKKTKNLSSNEAISLEEIRIRVVQMLGSLGGQINKNLLT
                     VTSSDEMMKSYVAWDREKRLSFAVPFREMKPVIFLDVFLPRVTELALTASDRQTKVAAC
                     ELLHSMVMFMLGKATQMPEGGQGAPPMYQLYKRTFPVLLRLACDVDQVTRQLYEPLVMQ
                     LIHWFTNNKKFESQDTVALLEAILDGIVDPVDSTLRDFCGRCIREFLKWSIKQITPQQQ
                     EKSPVNTKSLFKRLYSLALHPNAFKRLGASLAFNNIYREFREEESLVEQFVFEALVIYM
                     ESLALAHADEKSLGTIQQCCDAIDHLCRIIEKKHVSLNKAKKRRLPRGFPPSASLCLLD
                     LVKWLLAHCGRPQTECRHKSIELFYKFVPLLPGNRSPNLWLKDVLKEEGVSFLINTFEG
                     GGCGQPSGILAQPTLLYLRGPFSLQATLCWLDLLLAALECYNTFIGERTVGALQVLGTE
                     AQSSLLKAVAFFLESIAMHDIIAAEKCFGTGAAGNRTSPQEGERYNYSKCTVVVRIMEF
                     TTTLLNTSPEGWKLLKKDLCNTHLMRVLVQTLCEPASIGFNIGDVQVMAHLPDVCVNLM
                     KALKMSPYKDILETHLREKITAQSIEELCAVNLYGPDAQVDRSRLAAVVSACKQLHRAG
                     LLHNILPSQSTDLHHSVGTELLSLVYKGIAPGDERQCLPSLDLSCKQLASGLLELAFAF
                     GGLCERLVSLLLNPAVLSTASLGSSQGSVIHFSHGEYFYSLFSETINTELLKNLDLAVL
                     ELMQSSVDNTKMVSAVLNGMLDQSFRERANQKHQGLKLATTILQHWKKCDSWWAKDSPL
                     ETKMAVLALLAKILQIDSSVSFNTSHGSFPEVFTTYISLLADTKLDLHLKGQAVTLLPF
                     FTSLTGGSLEELRRVLEQLIVAHFPMQSREFPPGTPRFNNYVDCMKKFLDALELSQSPM
                     LLELMTEVLCREQQHVMEELFQSSFRRIARRGSCVTQVGLLESVYEMFRKDDPRLSFTR
                     QSFVDRSLLTLLWHCSLDALREFFSTIVVDAIDVLKSRFTKLNESTFDTQITKKMGYYK
                     ILDVMYSRLPKDDVHAKESKINQVFHGSCITEGNELTKTLIKLCYDAFTENMAGENQLL
                     ERRRLYHCAAYNCAISVICCVFNELKFYQGFLFSEKPEKNLLIFENLIDLKRRYNFPVE
                     VEVPMERKKKYIEIRKEAREAANGDSDGPSYMSSLSYLADSTLSEEMSQFDFSTGVQSY
                     SYSSQDPRPATGRFRRREQRDPTVHDDVLELEMDELNRHECMAPLTALVKHMHRSLGPP
                     QGEEDSVPRDLPSWMKFLHGKLGNPIVPLNIRLFLAKLVINTEEVFRPYAKHWLSPLLQ
                     LAASENNGGEGIHYMVVEIVATILSWTGLATPTGVPKDEVLANRLLNFLMKHVFHPKRA
                     VFRHNLEIIKTLVECWKDCLSIPYRLIFEKFSGKDPNSKDNSVGIQLLGIVMANDLPPY
                     DPQCGIQSSEYFQALVNNMSFVRYKEVYAAAAEVLGLILRYVIERKNILEESLCELVAK
                     QLKQHQNTMEDKFIVCLNKVTKSFPPLADRFMNAVFFLLPKFHGVLKTLCLEVVLCRVE
                     GMTELYFQLKSKDFVQVMRHRDDERQKVCLDIIYKMMPKLKPVELRELLNPVVEFVSHP
                     STTCREQMYNILMWIHDNYRDPESETDNDSQEIFKLAKDVLIQGLIDENPGLQLIIRNF
                     WSHETRLPSNTLDRLLALNSLYSPKIEVHFLSLATNFLLEMTSMSPDYPNPMFEHPLSE
                     CEFQEYTIDSDWRFRSTVLTPMFVETQASQGTLQARTQEGSLSARWPVAGQIRATQQQH
                     DFTLTQTADGRSSFDWLTGSSTDPLVDHTSPSSDSLLFAHKRSERLQRAPLKSVGPDFG
                     KKRLGLPGDEVDNKVKGAAGRTDLLRLRRRFMRDQEKLSLMYARKGVAEQKREKEIKSE
                     LKMKQDAQVVLYRSYRHGDLPDIQIKHSSLITPLQAVAQRDPIIAKQLFSSLFSGILKE
                     MDKFKTLSEKNNITQKLLQDFNRFLNTTFSFFPPFVSCIQDISCQHAALLSLDPAAVSA
                     GCLASLQQPVGIRLLEEALLCLLPAELPAKRVRGKARPPPDVLRWVELAKLYRSIGEYD
                     VLRGIFTSEIGTKQITQSALLAEARSDYSEAAKQYDEALNKQDWVDGEPTEAEKDFWEL
                     ASLDCYNHLAEWKSLEYCSTASIDSENPPDLNKIWSEPFYQETYLPYMIRSKLKLLLQG
                     EADQSLLTFIDKAMHGELQKAILELHYSQELSLLYLLQDDVDRAKYYIQNGIQSFMQNY
                     SSIDVLLHQSRLTKLQSVQALTEIQEFISFISKQGNLSSQVPLKRLLNTWANRYPDAKM
                     DPMNIWDDIITNRCFFLSKIEEKLTPLPEDNSMNVDQDGDPSDRMEVQEQEEDISSLIR
                     SCKFSMKMKMIDSARKQNNFSLAMKLLKELHKESKTRDDWLVSWVQSYCRLSHCRSRSQ
                     GCSEQVLTVLKTVSLLDENNVSSYLSKNILAFRDQNILLGTTYRIIANALSSEPACLAE
                     IEEDKARRILELSGSSSEDSEKVIAGLYQRAFQHLSEAVQAAEEEAQPPSWSCGPAAGV
                     IDAYMTLADFCDQQLRKEEENASVTDSAELQAYPALVVEKMLKALKLNSNEARLKFPRL
                     LQIIERYPEETLSLMTKEISSVPCWQFISWISHMVALLDKDQAVAVQHSVEEITDNYPQ
                     AIVYPFIISSESYSFKDTSTGHKNKEFVARIKSKLDQGGVIQDFINALDQLSNPELLFK
                     DWSNDVRAELAKTPVNKKNIEKMYERMYAALGDPKAPGLGAFRRKFIQTFGKEFDKHFG
                     KGGSKLLRMKLSDFNDITNMLLLKMNKDSKPPGNLKECSPWMSDFKVEFLRNELEIPGQ
                     YDGRGKPLPEYHVRIAGFDERVTVMASLRRPKRIIIRGHDEREHPFLVKGGEDLRQDQR
                     VEQLFQVMNGILAQDSACSQRALQLRTYSVVPMTSRLGLIEWLENTVTLKDLLLNTMSQ
                     EEKAAYLSDPRAPPCEYKDWLTKMSGKHDVGAYMLMYKGANRTETVTSFRKRESKVPAD
                     LLKRAFVRMSTSPEAFLALRSHFASSHALICISHWILGIGDRHLNNFMVAMETGGVIGI
                     DFGHAFGSATQFLPVPELMPFRLTRQFINLMLPMKETGLMYSIMVHALRAFRSDPGLLT
                     NTMDVFVKEPSFDWKNFEQKMLKKGGSWIQEINVAEKNWYPRQKICYAKRKLAGANPAV
                     ITCDELLLGHEKAPAFRDYVAVARGSKDHNIRAQEPESGLSEETQVKCLMDQATDPNIL
                     GRTWEGWEPWM"
     CDS             3052..3117
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /note="color: #cc99b2
                     Cleavage site after base 3117"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    3118..3151
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
                     /note="color: #31849b; direction: RIGHT"
     CDS             3160..4050
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /note="color: #cc99b2"
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             4063..4083
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /note="color: #cc99b2
                     Cleavage site after base 4080"
                     /translation="EDLYFQS"
     polyA_signal    16543..16767
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     protein_bind    complement(16805..16838)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
                     /note="color: #31849b; direction: LEFT"
     primer_bind     complement(16871..16887)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(16920..16936)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
ORIGIN
        1 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
       61 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
      121 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
      181 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
      241 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
      301 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
      361 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
      421 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
      481 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
      541 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
      601 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
      661 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
      721 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
      781 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
      841 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
      901 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
      961 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     1021 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     1081 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     1141 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
     2041 catctgtgcg gtatttcaca ccgcataagc ggataacaat ttcacacagg aaacagctat
     2101 gaccatgatt acgccaagct tgcatgcctg caggggataa cttcgtataa tgtatgctat
     2161 acgaagttat ggggccgctc tagcctcgag gccgtaatga gacgcacaaa ctaatatcac
     2221 aaactggaaa tgtctatcaa tatatagttg ctctagttat taatagtaat caattacggg
     2281 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     2341 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     2401 agtaacgcca atagggactt tccattgacg tcaatgggtg gactatttac ggtaaactgc
     2461 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     2521 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     2581 gcagtacatc tacgtattag tcatcgctat taccatgcat ggtcgaggtg agccccacgt
     2641 tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt
     2701 tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggggcgcgcg ccaggcgggg
     2761 cggggcgggg cgaggggcgg ggcggggcga ggcggagagg tgcggcggca gccaatcaga
     2821 gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa
     2881 agcgaagcgc gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg
     2941 ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg
     3001 ggcgggacgg cccttctcct tcgggctgta attagcatca cgcgtaccat ggactacaaa
     3061 gaccatgacg gtgattataa agatcatgat atcgattaca aggatgacga tgacaagata
     3121 acttcgtata atgtatgcta tacgaagtta tccggtacca tggcagaaat cggtactggc
     3181 tttccattcg acccccatta tgtggaagtc ctgggcgagc gcatgcacta cgtcgatgtt
     3241 ggtccgcgcg atggcacccc tgtgctgttc ctgcacggta acccgacctc ctcctacgtg
     3301 tggcgcaaca tcatcccgca tgttgcaccg acccatcgct gcattgctcc agacctgatc
     3361 ggtatgggca aatccgacaa accagacctg ggttatttct tcgacgacca cgtccgcttc
     3421 atggatgcct tcatcgaagc cctgggtctg gaagaggtcg tcctggtcat tcacgactgg
     3481 ggctccgctc tgggtttcca ctgggccaag cgcaatccag agcgcgtcaa aggtattgca
     3541 tttatggagt tcatccgccc tatcccgacc tgggacgaat ggccagaatt tgcccgcgag
     3601 accttccagg ccttccgcac caccgacgtc ggccgcaagc tgatcatcga tcagaacgtt
     3661 tttatcgagg gtacgctgcc gatgggtgtc gtccgcccgc tgactgaagt cgagatggac
     3721 cattaccgcg agccgttcct gaatcctgtt gaccgcgagc cactgtggcg cttcccaaac
     3781 gagctgccaa tcgccggtga gccagcgaac atcgtcgcgc tggtcgaaga atacatggac
     3841 tggctgcacc agtcccctgt cccgaagctg ctgttctggg gcaccccagg cgttctgatc
     3901 ccaccggccg aagccgctcg cctggccaaa agcctgccta actgcaaggc tgtggacatc
     3961 ggcccgggtc tgaatctgct gcaagaagac aacccggacc tgatcggcag cgagatcgcg
     4021 cgctggctgt cgacgctcga gatttccggc gagccaacca ctgaggatct gtactttcag
     4081 agcgataacg cgatcgcttc cggtaccatg gcaggaagtg gagcaggtgt gagatgcagt
     4141 ctactaagac tacaagaaac cttaagtgct gcggaccgct gcggtgctgc cctggccggt
     4201 catcaactga tccgcggcct ggggcaggaa tgcgtcctga gcagcagccc cgcggtgctg
     4261 gcattacaga catctttagt tttttccaga gatttcggtt tgcttgtatt tgtccggaag
     4321 tcactcaaca gtattgaatt tcgtgaatgt agagaagaaa tcctaaagtt tttatgtatt
     4381 ttcttagaaa aaatgggcca gaagatcgca ccttactctg ttgaaattaa gaacacttgt
     4441 accagtgttt atacaaaaga tagagctgct aaatgtaaaa ttccagccct ggaccttctt
     4501 attaagttac ttcagacttt tagaagttct agactcatgg atgaatttaa aattggagaa
     4561 ttatttagta aattctatgg agaacttgca ttgaaaaaaa aaataccaga tacagtttta
     4621 gaaaaagtat atgagctcct aggattattg ggtgaagttc atcctagtga gatgataaat
     4681 aatgcagaaa acctgttccg cgcttttctg ggtgaactta agacccagat gacatcagca
     4741 gtaagagagc ccaaactacc tgttctggca ggatgtctga aggggttgtc ctcacttctg
     4801 tgcaacttca ctaagtccat ggaagaagat ccccagactt caagggagat ttttaatttt
     4861 gtactaaagg caattcgtcc tcagattgat ctgaagagat atgctgtgcc ctcagctggc
     4921 ttgcgcctat ttgccctgca tgcatctcag tttagcacct gccttctgga caactacgtg
     4981 tctctatttg aagtcttgtt aaagtggtgt gcccacacaa atgtagaatt gaaaaaagct
     5041 gcactttcag ccctggaatc ctttctgaaa caggtttcta atatggtggc gaaaaatgca
     5101 gaaatgcata aaaataaact gcagtacttt atggagcagt tttatggaat catcagaaat
     5161 gtggattcga acaacaagga gttatctatt gctatccgtg gatatggact ttttgcagga
     5221 ccgtgcaagg ttataaacgc aaaagatgtt gacttcatgt acgttgagct cattcagcgc
     5281 tgcaagcaga tgttcctcac ccagacagac actggtgacg accgtgttta tcagatgcca
     5341 agcttcctcc agtctgttgc aagcgtcttg ctgtaccttg acacagttcc tgaggtgtat
     5401 actccagttc tggagcacct cgtggtgatg cagatagaca gtttcccaca gtacagtcca
     5461 aaaatgcagc tggtgtgttg cagagccata gtgaaggtgt tcctagcttt ggcagcaaaa
     5521 gggccagttc tcaggaattg cattagtact gtggtgcatc agggtttaat cagaatatgt
     5581 tctaaaccag tggtccttcc aaagggacct gagtctgaat ctgaagacca ccgtgcttca
     5641 ggggaagtca gaactggcaa atggaaggtg cccacataca aagactacgt ggatctcttc
     5701 agacatctcc tgagctctga ccagatgatg gattctattt tagcagatga agcatttttc
     5761 tctgtgaatt cctccagtga aagtctgaat catttacttt atgatgaatt tgtaaaatcc
     5821 gttttgaaga ttgttgagaa attggatctt acacttgaaa tacagactgt tggggaacaa
     5881 gagaatggag atgaggcacc tggtgtttgg atgatcccaa cttcagatcc agcggctaac
     5941 ttgcatccag ctaaacctaa agatttttcg gctttcatta acctggtgga attttgcaga
     6001 gagattctcc ctgagaaaca agcagaattt tttgaaccat gggtgtactc attttcatat
     6061 gaattaattt tgcaatctac aaggttgccc ctcatcagtg gtttctacaa attgctttct
     6121 attacagtaa gaaatgccaa gaaaataaaa tatttcgagg gagttagtcc aaagagtctg
     6181 aaacactctc ctgaagaccc agaaaagtat tcttgctttg ctttatttgt gaaatttggc
     6241 aaagaggtgg cagttaaaat gaagcagtac aaagatgaac ttttggcctc ttgtttgacc
     6301 tttcttctgt ccttgccaca caacatcatt gaactcgatg ttagagccta cgttcctgca
     6361 ctgcagatgg ctttcaaact gggcctgagc tataccccct tggcagaagt aggactgaat
     6421 gctctagaag aatggtcaat ttatattgac agacatgtaa tgcagcctta ttacaaagac
     6481 attctcccct gcctggatgg atacctgaag acttcagcct tgtcagatga gaccaagaat
     6541 aactgggaag tgtcagctct ttctcgggct gcccagaaag gatttaataa agtggtgtta
     6601 aagcatctga agaagacaaa gaacctttca tcaaacgaag caatatcctt agaagaaata
     6661 agaattagag tagtacaaat gcttggatct ctaggaggac aaataaacaa aaatcttctg
     6721 acagtcacgt cctcagatga gatgatgaag agctatgtgg cctgggacag agagaagcgg
     6781 ctgagctttg cagtgccctt tagagagatg aaacctgtca ttttcctgga tgtgttcctg
     6841 cctcgagtca cagaattagc gctcacagcc agtgacagac aaactaaggt tgcagcctgt
     6901 gaacttttac atagcatggt tatgtttatg ttgggcaaag ccacgcagat gccagaaggg
     6961 ggacagggag ccccacccat gtaccagctc tataagcgga cgtttcctgt gctgcttcga
     7021 cttgcgtgtg atgttgatca ggtgacaagg caactgtatg agccactagt tatgcagctg
     7081 attcactggt tcactaacaa caagaaattt gaaagtcagg atactgttgc cttactagaa
     7141 gctatattgg atggaattgt ggaccctgtt gacagtactt taagagattt ttgtggtcgg
     7201 tgtattcgag aattccttaa atggtccatt aagcaaataa caccacagca gcaggagaag
     7261 agtccagtaa acaccaaatc gcttttcaag cgactttata gccttgcgct tcaccccaat
     7321 gctttcaaga ggctgggagc atcacttgcc tttaataata tctacaggga attcagggaa
     7381 gaagagtctc tggtggaaca gtttgtgttt gaagccttgg tgatatacat ggagagtctg
     7441 gccttagcac atgcagatga gaagtcctta ggtacaattc aacagtgttg tgatgccatt
     7501 gatcacctat gccgcatcat tgaaaagaag catgtttctt taaataaagc aaagaaacga
     7561 cgtttgccgc gaggatttcc accttccgca tcattgtgtt tattggatct ggtcaagtgg
     7621 cttttagctc attgtgggag gccccagaca gaatgtcgac acaaatccat tgaactcttt
     7681 tataaattcg ttcctttatt gccaggcaac agatccccta atttgtggct gaaagatgtt
     7741 ctcaaggaag aaggtgtctc ttttctcatc aacacctttg aggggggtgg ctgtggccag
     7801 ccctcgggca tcctggccca gcccaccctc ttgtaccttc gggggccatt cagcctgcag
     7861 gccacgctat gctggctgga cctgctcctg gccgcgttgg agtgctacaa cacgttcatt
     7921 ggcgagagaa ctgtaggagc gctccaggtc ctaggtactg aagcccagtc ttcacttttg
     7981 aaagcagtgg ctttcttctt agaaagcatt gccatgcatg acattatagc agcagaaaag
     8041 tgctttggca ctggggcagc aggtaacaga acaagcccac aagagggaga aaggtacaac
     8101 tacagcaaat gcaccgttgt ggtccggatt atggagttta ccacgactct gctaaacacc
     8161 tccccggaag gatggaagct cctgaagaag gacttgtgta atacacacct gatgagagtc
     8221 ctggtgcaga cgctgtgtga gcccgcaagc ataggtttca acatcggaga cgtccaggtt
     8281 atggctcatc ttcctgatgt ttgtgtgaat ctgatgaaag ctctaaagat gtccccatac
     8341 aaagatatcc tagagaccca tctgagagag aaaataacag cacagagcat tgaggagctt
     8401 tgtgccgtca acttgtatgg ccctgacgcg caagtggaca ggagcaggct ggctgctgtt
     8461 gtgtctgcct gtaaacagct tcacagagct gggcttctgc ataatatatt accgtctcag
     8521 tccacagatt tgcatcattc tgttggcaca gaacttcttt ccctggttta taaaggcatt
     8581 gcccctggag atgagagaca gtgtctgcct tctctagacc tcagttgtaa gcagctggcc
     8641 agcggacttc tggagttagc ctttgctttt ggaggactgt gtgagcgcct tgtgagtctt
     8701 ctcctgaacc cagcggtgct gtccacggcg tccttgggca gctcacaggg cagcgtcatc
     8761 cacttctccc atggggagta tttctatagc ttgttctcag aaacgatcaa cacggaatta
     8821 ttgaaaaatc tggatcttgc tgtattggag ctcatgcagt cttcagtgga taataccaaa
     8881 atggtgagtg ccgttttgaa cggcatgtta gaccagagct tcagggagcg agcaaaccag
     8941 aaacaccaag gactgaaact tgcgactaca attctgcaac actggaagaa gtgtgattca
     9001 tggtgggcca aagattcccc tctcgaaact aaaatggcag tgctggcctt actggcaaaa
     9061 attttacaga ttgattcatc tgtatctttt aatacaagtc atggttcatt ccctgaagtc
     9121 tttacaacat atattagtct acttgctgac acaaagctgg atctacattt aaagggccaa
     9181 gctgtcactc ttcttccatt cttcaccagc ctcactggag gcagtctgga ggaacttaga
     9241 cgtgttctgg agcagctcat cgttgctcac ttccccatgc agtccaggga atttcctcca
     9301 ggaactccgc ggttcaataa ttatgtggac tgcatgaaaa agtttctaga tgcattggaa
     9361 ttatctcaaa gccctatgtt gttggaattg atgacagaag ttctttgtcg ggaacagcag
     9421 catgtcatgg aagaattatt tcaatccagt ttcaggagga ttgccagaag gggttcatgt
     9481 gtcacacaag taggccttct ggaaagcgtg tatgaaatgt tcaggaagga tgacccccgc
     9541 ctaagtttca cacgccagtc ctttgtggac cgctccctcc tcactctgct gtggcactgt
     9601 agcctggatg ctttgagaga attcttcagc acaattgtgg tggatgccat tgatgtgttg
     9661 aagtccaggt ttacaaagct aaatgaatct acctttgata ctcaaatcac caagaagatg
     9721 ggctactata agattctaga cgtgatgtat tctcgccttc ccaaagatga tgttcatgct
     9781 aaggaatcaa aaattaatca agttttccat ggctcgtgta ttacagaagg aaatgaactt
     9841 acaaagacat tgattaaatt gtgctacgat gcatttacag agaacatggc aggagagaat
     9901 cagctgctgg agaggagaag actttaccat tgtgcagcat acaactgcgc catatctgtc
     9961 atctgctgtg tcttcaatga gttaaaattt taccaaggtt ttctgtttag tgaaaaacca
    10021 gaaaagaact tgcttatttt tgaaaatctg atcgacctga agcgccgcta taattttcct
    10081 gtagaagttg aggttcctat ggaaagaaag aaaaagtaca ttgaaattag gaaagaagcc
    10141 agagaagcag caaatgggga ttcagatggt ccttcctata tgtcttccct gtcatatttg
    10201 gcagacagta ccctgagtga ggaaatgagt caatttgatt tctcaaccgg agttcagagc
    10261 tattcataca gctcccaaga ccctagacct gccactggtc gttttcggag acgggagcag
    10321 cgggacccca cggtgcatga tgatgtgctg gagctggaga tggacgagct caatcggcat
    10381 gagtgcatgg cgcccctgac ggccctggtc aagcacatgc acagaagcct gggcccgcct
    10441 caaggagaag aggattcagt gccaagagat cttccttctt ggatgaaatt cctccatggc
    10501 aaactgggaa atccaatagt accattaaat atccgtctct tcttagccaa gcttgttatt
    10561 aatacagaag aggtctttcg cccttacgcg aagcactggc ttagcccctt gctgcagctg
    10621 gctgcttctg aaaacaatgg aggagaagga attcactaca tggtggttga gatagtggcc
    10681 actattcttt catggacagg cttggccact ccaacagggg tccctaaaga tgaagtgtta
    10741 gcaaatcgat tgcttaattt cctaatgaaa catgtctttc atccaaaaag agctgtgttt
    10801 agacacaacc ttgaaattat aaagaccctt gtcgagtgct ggaaggattg tttatccatc
    10861 ccttataggt taatatttga aaagttttcc ggtaaagatc ctaattctaa agacaactca
    10921 gtagggattc aattgctagg catcgtgatg gccaatgacc tgcctcccta tgacccacag
    10981 tgtggcatcc agagtagcga atacttccag gctttggtga ataatatgtc ctttgtaaga
    11041 tataaagaag tgtatgccgc tgcagcagaa gttctaggac ttatacttcg atatgttata
    11101 gagagaaaaa acatactgga ggagtctctg tgtgaactgg ttgcgaaaca attgaagcaa
    11161 catcagaata ctatggagga caagtttatt gtgtgcttga acaaagtgac caagagcttc
    11221 cctcctcttg cagacaggtt catgaatgct gtgttctttc tgctgccaaa atttcatgga
    11281 gtgttgaaaa cactctgtct ggaggtggta ctttgtcgtg tggagggaat gacagagctg
    11341 tacttccagt taaagagcaa ggacttcgtt caagtcatga gacatagaga tgatgaaaga
    11401 caaaaagtat gtttggacat aatttataag atgatgccaa agttaaaacc agtagaactc
    11461 cgagaacttc tgaaccccgt tgtggaattc gtttcccatc cttctacaac atgtagggaa
    11521 caaatgtata atattctcat gtggattcat gataattaca gagatccaga aagtgagaca
    11581 gataatgact cccaggaaat atttaagttg gcaaaagatg tgctgattca aggattgatc
    11641 gatgagaacc ctggacttca attaattatt cgaaatttct ggagccatga aactaggtta
    11701 ccttcaaata ccttggaccg gttgctggca ctaaattcct tatattctcc taagatagaa
    11761 gtgcactttt taagtttagc aacaaatttt ctgctcgaaa tgaccagcat gagcccagat
    11821 tatccaaacc ccatgttcga gcatcctctg tcagaatgcg aatttcagga atataccatt
    11881 gattctgatt ggcgtttccg aagtactgtt ctcactccga tgtttgtgga gacccaggcc
    11941 tcccagggca ctctccaggc ccgtacccag gaagggtccc tctcagctcg ctggccagtg
    12001 gcagggcaga taagggccac ccagcagcag catgacttca cactgacaca gactgcagat
    12061 ggaagaagct catttgattg gctgaccggg agcagcactg acccgctggt cgaccacacc
    12121 agtccctcat ctgactcctt gctgtttgcc cacaagagga gtgaaaggtt acagagagca
    12181 cccttgaagt cagtggggcc tgattttggg aaaaaaaggc tgggccttcc aggggacgag
    12241 gtggataaca aagtgaaagg tgcggccggc cggacggacc tactacgact gcgcagacgg
    12301 tttatgaggg accaggagaa gcttagtttg atgtatgcca gaaaaggcgt tgctgagcaa
    12361 aaacgagaga aggaaatcaa gagtgagtta aaaatgaagc aggatgccca ggtcgttctg
    12421 tacagaagct accggcacgg agaccttcct gacattcaga tcaagcacag cagcctcatc
    12481 accccgttac aggccgtggc ccagagggac ccaataatcg cgaaacagct ctttagcagc
    12541 ttgttttctg gaattttgaa agagatggat aaatttaaga cactgtctga aaaaaacaac
    12601 atcactcaaa agttgcttca agacttcaat cgttttctta ataccacctt ctctttcttt
    12661 ccaccctttg tctcttgtat tcaggacatt agctgtcagc acgcagccct gctgagcctc
    12721 gacccagcgg ctgttagcgc tggttgcctg gccagcctac agcagcctgt tggcatccgc
    12781 ctactagagg aggctcttct ttgtctgctg cctgctgagc tgcccgccaa gcgagtccgt
    12841 gggaaggcta gacctcctcc tgatgtcctc agatgggtgg agcttgctaa gctgtataga
    12901 tcaattggag aatacgacgt cctccgtggg atttttacca gtgagatagg aacaaagcaa
    12961 atcactcaga gtgcattatt agcagaagcc agaagtgatt attctgaagc tgctaagcag
    13021 tatgatgagg ctctcaataa acaagactgg gtagatggtg agcccacaga agccgagaag
    13081 gatttttggg aacttgcatc ccttgactgt tacaaccacc ttgctgagtg gaaatcactt
    13141 gaatactgtt ctacagccag tatagacagt gagaaccccc cagacctaaa taaaatctgg
    13201 agtgaaccat tttatcagga aacatatcta ccttacatga tccgcagcaa gctgaagctg
    13261 ctgctccagg gagaggctga ccagtccctg ctgacattta ttgacaaagc tatgcacggg
    13321 gagctccaga aggcgattct agagcttcat tacagtcaag agctgagtct gctttacctc
    13381 ctgcaagatg atgttgacag agccaaatat tacattcaaa atggcattca gagttttatg
    13441 cagaattatt ctagtattga tgtcctctta caccaaagta gactcaccaa attgcagtct
    13501 gtacaggctt taacagaaat tcaggagttc atcagcttta taagcaaaca aggcaattta
    13561 tcatctcaag ttccccttaa gagacttctg aacacctggg caaacagata tccagatgct
    13621 aaaatggacc caatgaacat ctgggatgac atcatcacaa atcgatgttt ctttctcagc
    13681 aaaatagagg agaagcttac ccctcttcca gaagataata gtatgaatgt ggatcaagat
    13741 ggagacccca gtgacaggat ggaagtgcaa gagcaggaag aagatatcag ctccctgatc
    13801 aggagttgca agttttccat gaaaatgaag atgatagaca gtgcccggaa gcagaacaat
    13861 ttctcacttg ctatgaaact actgaaggag ctgcataaag agtcaaaaac cagagacgat
    13921 tggctggtga gctgggtgca gagctactgc cgcctgagcc actgccggag ccggtcccag
    13981 ggctgctctg agcaggtgct cactgtgctg aaaacagtct ctttgttgga tgagaacaac
    14041 gtgtcaagct acttaagcaa aaatattctg gctttccgtg accagaacat tctcttgggt
    14101 acaacttaca ggatcatagc gaatgctctc agcagtgagc cagcctgcct tgctgaaatc
    14161 gaggaggaca aggctagaag aatcttagag ctttctggat ccagttcaga ggattcagag
    14221 aaggtgatcg cgggtctgta ccagagagca ttccagcacc tctctgaggc tgtgcaggcg
    14281 gctgaggagg aggcccagcc tccctcctgg agctgtgggc ctgcagctgg ggtgattgat
    14341 gcttacatga cgctggcaga tttctgtgac caacagctgc gcaaggagga agagaatgca
    14401 tcagttactg attctgcaga actgcaggcg tatccagcac ttgtggtgga gaaaatgttg
    14461 aaagctttaa aattaaattc caatgaagcc agattgaagt ttcctagatt acttcagatt
    14521 atagaacggt atccagagga gactttgagc ctcatgacaa aagagatctc ttccgttccc
    14581 tgctggcagt tcatcagctg gatcagccac atggtggcct tactggacaa agaccaagcc
    14641 gttgctgttc agcactctgt ggaagaaatc actgataact acccgcaggc tattgtttat
    14701 cccttcatca taagcagcga aagctattcc ttcaaggata cttctactgg tcataagaat
    14761 aaggagtttg tggcaaggat taaaagtaag ttggatcaag gaggagtgat tcaagatttt
    14821 attaatgcct tagatcagct ctctaatcct gaactgctct ttaaggattg gagcaatgat
    14881 gtaagagctg aactagcaaa aacccctgta aataaaaaaa acattgaaaa aatgtatgaa
    14941 agaatgtatg cagccttggg tgacccaaag gctccaggcc tgggggcctt tagaaggaag
    15001 tttattcaga cttttggaaa agaatttgat aaacattttg ggaaaggagg ttctaaacta
    15061 ctgagaatga agctcagtga cttcaacgac attaccaaca tgctactttt aaaaatgaac
    15121 aaagactcaa agccccctgg gaatctgaaa gaatgttcac cctggatgag cgacttcaaa
    15181 gtggagttcc tgagaaatga gctggagatt cccggtcagt atgacggtag gggaaagcca
    15241 ttgccagagt accacgtgcg aatcgccggg tttgatgagc gggtgacagt catggcgtct
    15301 ctgcgaaggc ccaagcgcat catcatccgt ggccatgacg agagggaaca ccctttcctg
    15361 gtgaagggtg gcgaggacct gcggcaggac cagcgcgtgg agcagctctt ccaggtcatg
    15421 aatgggatcc tggcccaaga ctccgcctgc agccagaggg ccctgcagct gaggacctat
    15481 agcgttgtgc ccatgacctc caggttagga ttaattgagt ggcttgaaaa tactgttacc
    15541 ttgaaggacc ttcttttgaa caccatgtcc caagaggaga aggcggctta cctgagtgat
    15601 cccagggcac cgccgtgtga atataaagat tggctgacaa aaatgtcagg aaaacatgat
    15661 gttggagctt acatgctaat gtataagggc gctaatcgta ctgaaacagt cacgtctttt
    15721 agaaaacgag aaagtaaagt gcctgctgat ctcttaaagc gggccttcgt gaggatgagt
    15781 acaagccctg aggctttcct ggcgctccgc tcccacttcg ccagctctca cgctctgata
    15841 tgcatcagcc actggatcct cgggattgga gacagacatc tgaacaactt tatggtggcc
    15901 atggagactg gtggcgtgat cgggatcgac tttgggcatg cgtttggatc cgctacacag
    15961 tttctgccag tccctgagtt gatgcctttt cggctaactc gccagtttat caatctgatg
    16021 ttaccaatga aagaaacggg ccttatgtac agcatcatgg tacacgcact ccgggccttc
    16081 cgctcagacc ctggcctgct caccaacacc atggatgtgt ttgtcaagga gccctccttt
    16141 gattggaaaa attttgaaca gaaaatgctg aaaaaaggag ggtcatggat tcaagaaata
    16201 aatgttgctg aaaaaaattg gtacccccga cagaaaatat gttacgctaa gagaaagtta
    16261 gcaggtgcca atccagcagt cattacttgt gatgagctac tcctgggtca tgagaaggcc
    16321 cctgccttca gagactatgt ggctgtggca cgaggaagca aagatcacaa cattcgtgcc
    16381 caagaaccag agagtgggct ttcagaagag actcaagtga agtgcctgat ggaccaggca
    16441 acagacccca acatccttgg cagaacctgg gaaggatggg agccctggat gtgaggtctg
    16501 cggccgcgat ctgtcgacga taaacccgct gatcagcctc gactgtgcct tctagttgcc
    16561 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
    16621 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
    16681 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
    16741 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccaga tcggatccat
    16801 cgatataact tcgtatagga gactttatac gaagttatct cgatgaaggg gttctctaga
    16861 tcgagaattc actggccgtc gttttacaag gcgcgccccc gggtaccgag ctcgaattca
    16921 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcg
//
