LOCUS       Exported                8324 bp ds-DNA     circular SYN 23-OCT-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8324)
  AUTHORS   skyou
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Oct 23, 2023 from SnapGene 4.3.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8324
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        471..575
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             576..1436
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1:  576 ..  644 / #ccffcc / signal sequence
                        2:  645 .. 1436 / #ccffcc
                     Cleavage site after base 644"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(597..615)
                     /label=Amp-F
                     /note="color: black; sequence: AAAAGGGAATAAGGGCGAC"
     primer_bind     1378..1397
                     /label=Amp-R
                     /note="color: black; sequence: TGGATGAACGAAATAGACAG"
     rep_origin      1607..2195
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    2483..2504
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     primer_bind     2489..2509
                     /label=3.0rev
                     /note="21"
                     /note="color: black; sequence: GAGTTAGCTCACTCATTAGGC"
     primer_bind     2513..2532
                     /label=P1
                     /note="20"
                     /note="color: black; sequence: CCAGGCTTTACACTTTATGC"
     primer_bind     2516..2535
                     /label=pTarget.R
                     /note="20"
                     /note="color: black; sequence: GGCTTTACACTTTATGCTTC"
     promoter        2519..2549
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 2519 .. 2524 / #ffffff / -35
                        2: 2525 .. 2542 / #ffffff
                        3: 2543 .. 2549 / #ffffff / -10"
     primer_bind     2527..2546
                     /label=Lac-F
                     /note="20"
                     /note="color: black; sequence: TTATGCTTCCGGCTCGTATG"
     primer_bind     2553..2571
                     /label=PETT7
                     /note="20"
                     /note="color: black; sequence: GGGAATTGTGAGCGGATAAC"
     protein_bind    2557..2573
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     2560..2579
                     /label=pQE30-F
                     /note="20"
                     /note="color: black; sequence: TGAGCGGATAACAATTTCAC"
     primer_bind     2562..2585
                     /label=M13R(-48)
                     /note="24"
                     /note="color: black; sequence: AGCGGATAACAATTTCACACAGGA"
     primer_bind     2562..2583
                     /label=PQE30-F
                     /note="22"
                     /note="color: black; sequence: AGCGGATAACAATTTCACACAG"
     primer_bind     2565..2584
                     /label=PBI101-F
                     /note="20"
                     /note="color: black; sequence: GGATAACAATTTCACACAGG"
     primer_bind     2581..2598
                     /label=M13R
                     /note="18"
                     /note="color: black; sequence: CAGGAAACAGCTATGACC"
     primer_bind     2581..2597
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        2618..2636
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     2619..2638
                     /label=T3
                     /note="20"
                     /note="color: black; sequence: ATTAACCCTCACTAAAGGGA"
     primer_bind     2628..2677
                     /label=363 FGF8b LA-5'
                     /note="color: black; sequence: 
                     cactaaagggaacaaaagctggtaccagtgagggagaaggaagctgtttg"
     misc_feature    2654..3633
                     /label=FGF8 left arm
                     /note="color: #ff9900"
     primer_bind     complement(3281..3292)
                     /label=pZeroBack-F
                     /note="23"
                     /note="color: black; sequence: CGACTCACTATAGGGAGAGCGGC"
     primer_bind     complement(3281..3292)
                     /label=pJET-F
                     /note="23"
                     /note="color: black; sequence: CGACTCACTATAGGGAGAGCGGC"
     primer_bind     complement(3614..3653)
                     /label=364 FGF8b-LA-3'
                     /note="color: black; sequence: 
                     AAGTTTGTTGCGCCGGATCCtcggggctcgggggcccaag"
     misc_feature    3634..3699
                     /label=P2A
                     /note="color: #00ff00; direction: RIGHT"
     primer_bind     3634..3653
                     /label=368 iCre-PGK-puro-5'
                     /note="color: black; sequence: GGATCCGGCGCAACAAACTT"
     misc_feature    3700..4758
                     /label=iCre
                     /note="This forward directional feature has 3 segments:
                        1: 3700 .. 3705 / #ff00ff
                        2: 3706 .. 3726 / #993366 / SV40 NLS
                        3: 3727 .. 4758 / #ff00ff"
     primer_bind     complement(3922..3941)
                     /label=381
                     /note="color: black; sequence: tccctcacatcctcaggttc"
     protein_bind    4765..4798
                     /label=vLOXP
                     /bound_moiety="vCre recombinase"
                     /note="color: #00ccff; direction: RIGHT"
     misc_feature    4799..5296
                     /label=hGH polyA
                     /note="color: #a6acb3"
     primer_bind     complement(4866..4883)
                     /label=pFlag-CMV.R
                     /note="18"
                     /note="color: black; sequence: GCACTGGAGTGGCAACTT"
     primer_bind     complement(4880..4903)
                     /label=CMV-24
                     /note="24"
                     /note="color: black; sequence: TATTAGGACAAGGCTGGTGGGCAC"
     primer_bind     complement(4883..4902)
                     /label=XL39
                     /note="20"
                     /note="color: black; sequence: ATTAGGACAAGGCTGGTGGG"
     primer_bind     complement(4888..4909)
                     /label=3Xflag.R
                     /note="22"
                     /note="color: black; sequence: TAATTTTATTAGGACAAGGCTG"
     primer_bind     complement(4925..4944)
                     /label=hGHPolyA
                     /note="20"
                     /note="color: black; sequence: TAGAAGGACACCTAGTCAGA"
     primer_bind     complement(4928..4948)
                     /label=Pcmv5r
                     /note="21"
                     /note="color: black; sequence: ATTATAGAAGGACACCTAGTC"
     promoter        5348..5860
                     /label=mouse PGK promoter
                     /note="color: #c040ff; direction: RIGHT"
     primer_bind     complement(5457..5476)
                     /label=PLVX-TRE3G-R
                     /note="color: black; sequence: GCCTACCGGTGGATGTGGAA"
     primer_bind     complement(5512..5530)
                     /label=PMSCV-RN
                     /note="19"
                     /note="color: black; sequence: GGGGAGGAGTAGAAGGTGG"
     primer_bind     complement(5620..5639)
                     /label=PMSCV-R
                     /note="20"
                     /note="color: black; sequence: CATTGCTCAGCGGTGCTGTC"
     primer_bind     complement(5627..5638)
                     /label=T7TER
                     /note="20"
                     /note="color: black; sequence: TGCTAGTTATTGCTCAGCGG"
     exon            5896..6495
                     /label=PUR
                     /note="color: #ff6600; direction: RIGHT"
     polyA_signal    6496..7051
                     /label=PolyA
                     /note="color: #008000"
     primer_bind     6813..6832
                     /label=386 pur-polyA-F
                     /note="color: black; sequence: attaagggccagctcattcc"
     protein_bind    7052..7085
                     /label=vLOXP
                     /bound_moiety="vCre recombinase"
                     /note="color: #00ccff; direction: RIGHT"
     primer_bind     complement(7077..7121)
                     /label=367 iCre-PGK-puro-3'
                     /note="color: black; sequence: 
                     tgtttcggtaaaggcagcacctaagactcaggatccTCAATTTCC"
     misc_feature    7092..7099
                     /label=LAMX1A RIGHT ARM
                     /note="color: #ff6600; direction: RIGHT"
     misc_feature    7092..7094
                     /label=termination
                     /note="color: #a6acb3"
     misc_feature    7099..8098
                     /label=fgf8 right arm
                     /note="color: #99cc00"
     primer_bind     7099..7173
                     /label=365 fgf8b RA-5'
                     /note="color: black; sequence: 
                     taggtgctgcctttaccgaaacacaatgccagaccgcagagaggctcatcggtgagggc
                     acccaaaactcaagca"
     misc_feature    7099..7101
                     /label=stop
                     /note="color: #ff0000"
     misc_feature    complement(7120..7135)
                     /label=FGF8b-sgRNA pair2-pre
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    complement(7120..7130)
                     /label=FGF8b-sgRNA pair1-pre
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    7133..7148
                     /label=FGF8b-sgRNA pair1/2-post
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(8079..8121)
                     /label=366 FGF8b-RA-3'
                     /note="color: black; sequence: 
                     agctccaccgcggtggcggccgcccccctccaatatgccccca"
     promoter        complement(8131..8149)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(8131..8149)
                     /label=T7
                     /note="19"
                     /note="color: black; sequence: TAATACGACTCACTATAGG"
     primer_bind     complement(8132..8149)
                     /label=T72G
                     /note="18"
                     /note="color: black; sequence: TAATACGACTCACTATAG"
     primer_bind     complement(8156..8173)
                     /label=M13F
                     /note="18"
                     /note="color: black; sequence: TGTAAAACGACGGCCAGT"
     primer_bind     complement(8156..8172)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(8176..8199)
                     /label=M13F(-47)
                     /note="24"
                     /note="color: black; sequence: CGCCAGGGTTTTCCCAGTCACGAC"
     primer_bind     complement(8176..8188)
                     /label=Petblue-2.f
                     /note="18"
                     /note="color: black; sequence: GATTCTCCCAGTCACGAC"
     primer_bind     complement(8179..8197)
                     /label=pTarget.F
                     /note="19"
                     /note="color: black; sequence: CCAGGATTTTCCCAGTCAC"
     primer_bind     complement(8197..8216)
                     /label=P2
                     /note="20"
                     /note="color: black; sequence: GCGATTAAGTTGGGTAACGC"
     primer_bind     complement(8198..8217)
                     /label=Lac-R
                     /note="20"
                     /note="color: black; sequence: GGCGATTAAGTTGGGTAACG"
     primer_bind     complement(8201..8218)
                     /label=2.0rev
                     /note="18"
                     /note="color: black; sequence: AGGCGATTAAGTTGGGTA"
     primer_bind     complement(8204..8223)
                     /label=PMAL-c2-R
                     /note="20"
                     /note="color: black; sequence: CTGCAAGGCGATTAAGTTGG"
     primer_bind     complement(8282..8303)
                     /label=PMD-F
                     /note="22"
                     /note="color: black; sequence: ATTCAGGCTGCGCAACTGTTGG"
     rep_origin      complement(join(8313..8324,1..444))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
ORIGIN
        1 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
       61 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
      121 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
      181 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
      241 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
      301 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
      361 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
      421 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
      481 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      541 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
      601 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
      661 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
      721 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
      781 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
      841 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
      901 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
      961 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     1021 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     1081 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     1141 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     1201 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     1261 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     1321 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     1381 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     1441 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     1501 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     1561 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     1621 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     1681 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     1741 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     1801 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     1861 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     1921 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     1981 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2041 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2101 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2161 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2221 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2281 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     2341 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     2401 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     2461 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     2521 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     2581 caggaaacag ctatgaccat gattacgcca agctcgaaat taaccctcac taaagggaac
     2641 aaaagctggt accagtgagg gagaaggaag ctgtttgaga gatgaaaact ataaaaggcg
     2701 aaggaggaag aatgaaggct agggaagggg ctggggggtg gtggttggta aattccatat
     2761 atctttttaa caaatcgcca aattgctttg ctattatgtc caattacatg gtaccagcat
     2821 ttggaatgtt cagtaagccg caggcccagc ccctcggccg agctctgaaa tggctccatt
     2881 agtcacggct cctctccagc ctcgagctcc ccacttcctg ggcttctggg gctggggtct
     2941 tagcatcttc tcccaggcct cccctccccc atagggtggc tgccctgggg ccagggaacc
     3001 gaagtcctgg gggggtgaga ggggcaggtg gggagacggg tggccagact ggtgggcagg
     3061 aggccagagc aggccaggct ctgggcccct ctctctgtct ttctgcgttg gggcccagcc
     3121 cctccgtaga caaccatgtg tcactgctgc ctgggaggac aggaagttgc cgggtgggct
     3181 gcgagttgtg agggattaga gagcgggtgc ccaggcaggg ggtggggctg cggctcctgc
     3241 ccacctcgcc atctgctggg gtgcccacct gctgtctggg gccgctcgcc ctctgcctct
     3301 gctggggggg ctctgtaacg tggtgtctgg ctcccctacc tgcagagcaa cggcaaaggc
     3361 aaggactgcg tcttcacgga gattgtgctg gagaacaact acacagcgct gcagaatgcc
     3421 aagtacgagg gctggtacat ggccttcacc cgcaagggcc ggccccgcaa gggctccaag
     3481 acgcggcagc accagcgtga ggtccacttc atgaagcggc tgccccgggg ccaccacacc
     3541 accgagcaga gcctgcgctt cgagttcctc aactacccgc ccttcacgcg cagcctgcgc
     3601 ggcagccaga ggacttgggc ccccgagccc cgaggatccg gcgcaacaaa cttctctctg
     3661 ctgaaacaag ccggagatgt cgaagagaat cctggaccga tggtgcccaa gaagaagagg
     3721 aaagtctcca acctgctgac tgtgcaccaa aacctgcctg ccctccctgt ggatgccacc
     3781 tctgatgaag tcaggaagaa cctgatggac atgttcaggg acaggcaggc cttctctgaa
     3841 cacacctgga agatgctcct gtctgtgtgc agatcctggg ctgcctggtg caagctgaac
     3901 aacaggaaat ggttccctgc tgaacctgag gatgtgaggg actacctcct gtacctgcaa
     3961 gccagaggcc tggctgtgaa gaccatccaa cagcacctgg gccagctcaa catgctgcac
     4021 aggagatctg gcctgcctcg cccttctgac tccaatgctg tgtccctggt gatgaggaga
     4081 atcagaaagg agaatgtgga tgctggggag agagccaagc aggccctggc ctttgaacgc
     4141 actgactttg accaagtcag atccctgatg gagaactctg acagatgcca ggacatcagg
     4201 aacctggcct tcctgggcat tgcctacaac accctgctgc gcattgccga aattgccaga
     4261 atcagagtga aggacatctc ccgcaccgat ggtgggagaa tgctgatcca cattggcagg
     4321 accaagaccc tggtgtccac agctggtgtg gagaaggccc tgtccctggg ggttaccaag
     4381 ctggtggaga gatggatctc tgtgtctggt gtggctgatg accccaacaa ctacctgttc
     4441 tgccgggtca gaaagaatgg tgtggctgcc ccttctgcca cctcccaact gtccacccgg
     4501 gccctggaag ggatctttga ggccacccac cgcctgatct atggtgccaa ggatgactct
     4561 gggcagagat acctggcctg gtctggccac tctgccagag tgggtgctgc cagggacatg
     4621 gccagggctg gtgtgtccat ccctgaaatc atgcaggctg gtggctggac caatgtgaac
     4681 attgtgatga actacatcag aaacctggac tctgagactg gggccatggt gaggctgctc
     4741 gaggatgggg actgatagga attctcaatt tctgagaact gtcattctcg gaaattgaag
     4801 cgctgctcga gagatctacg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc
     4861 cctggaagtt gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat
     4921 tttgtctgac taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa
     4981 ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag
     5041 tgcagtggca caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct
     5101 gcctcagcct cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt
     5161 gtttttttgg tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc
     5221 tcaggtgatc tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc
     5281 tcccttccct gtcctttagg tctgaagagg agtttacgtc cagccacgcg taggatctcg
     5341 acctcgaaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
     5401 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
     5461 acatccaccg gtaggcgcca accgactccg ttctttggtg gccccttcgc gccaccttct
     5521 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
     5581 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
     5641 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
     5701 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
     5761 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
     5821 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcatccatct agatctcgag
     5881 cagctgaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     5941 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     6001 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     6061 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     6121 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     6181 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     6241 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     6301 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     6361 gtgcccgcct tcctggagac ctccgcgccc cacaacctcc ccttctacga gcggctcggc
     6421 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     6481 aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga
     6541 ccccatgcat cgatgatatc agatccccgg gatgcagaaa ttgatgatct attaaacaat
     6601 aaagatgtcc actaaaatgg aagtttttcc tgtcatactt tgttaagaag ggtgagaaca
     6661 gagtacctac attttgaatg gaaggattgg agctacgggg gtgggggtgg ggtgggatta
     6721 gataaatgcc tgctctttac tgaaggctct ttactattgc tttatgataa tgtttcatag
     6781 ttggatatca taatttaaac aagcaaaacc aaattaaggg ccagctcatt cctcccactc
     6841 atgatctata gatctataga tctctcgtgg gatcattgtt tttctcttga ttcccacttt
     6901 gtggttctaa gtactgtggt ttccaaatgt gtcagtttca tagcctgaag aacgagatca
     6961 gcagcctctg ttccacatac acttcattct cagtattgtt ttgccaagtt ctaattccat
     7021 cagaagctgg tcgagatccg gaacccttaa ttcaatttct gagaactgtc attctcggaa
     7081 attgaggatc ctgagtctta ggtgctgcct ttaccgaaac acaatgccag accgcagaga
     7141 ggctcatcgg tgagggcacc caaaactcaa gcaagatgag ctgtgcgctg ctctgcaggc
     7201 tggggaggtg ctgggggagc cctgggttcc ggttgttgat attgtttgct gttgggtttt
     7261 tgctgttttt tttttttttt ttttttttaa aacaaaagag aggctctatt tttgtattcc
     7321 acttggctgt ggtgtctgtc ttcttaactc tcagaaagct ccattagtgg cctagactgg
     7381 gattccggct gggggtttgc gggggtgggg ggctttctct agcctgtgct gctgaggccc
     7441 cagtacctcc agggccagtt ggctgggcag ccagggactc cactgcaccc ccaggtgggg
     7501 cagggaggaa aggactgtga catagggcag tcctcttaga agtgggtatc agactggtgg
     7561 ctattaaatg attgaaatat ttatttaact tgcatattaa aaatgtgtgc tggagagtga
     7621 gtcctgccgg ggtcagcccc tccctccaac cttgccccag ctggtgggcg gctgggagac
     7681 gcagatgacc aggtgccagc tctgaccaca gcctccctcc agcctaaaga cacctgcctg
     7741 tcaaccatcc ccatcactgt cacttgaggg gttttcctgc aaggacagaa gcagggaaag
     7801 gggcaagaag aggctcttag ctagtccttg gagctctcag atgtgtacct cctagcactt
     7861 tacagaggtc attgctaaca cttccccagg ccacctcagg gccagaaata atggatgtgc
     7921 tagggctaga gctgtaatca tggatttaat cctcttaaaa agtgcttctc tgagtgccta
     7981 ggtccatgtg ggagacaggt tggagattcc agaacttgct ctttctgaga ctcaggctcc
     8041 agaaaatgaa agaaaagagc agctgccagg gtccaaggtg ggggcatatt ggaggggggc
     8101 ggccgccacc gcggtggagc tccaattcgc cctatagtga gtcgtattac aattcactgg
     8161 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     8221 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     8281 cccaacagtt gcgcagcctg aatggcgaat ggaaattgta agcg
//
