LOCUS       Exported               17239 bp ds-DNA     circular SYN 22-OCT-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Oct 23, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Imported FromPath: 
            /Users/arimura/Dropbox/柳瀬/柳瀬引き継ぎ20180401/シロイヌナズナ/TALEN作製関連/TALENベ
            クター　バーチャルFilename: 【DEST】pK7WG2DIPS_RPS5Apro - コピー.xdna
FEATURES             Location/Qualifiers
     source          1..17239
                     /organism="synthetic DNA construct"
                     /lab_host="synthetic DNA construct"
                     /mol_type="other DNA"
     source          13391..16987
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="/Transferred From=pMDCDIPS20181026作製途中ほぼ完成？"
                     /note="/Transferred Similarity=99.65%"
     misc_feature    22..1469
                     /label=pOLE1
                     /note="Geneious type: promoter uukaryotic"
     primer_bind     22..41
                     /label=pOLEFw
                     /note="/created by=arimura"
     CDS             1470..2228
                     /label=OLE1
     misc_feature    complement(2249..2269)
                     /label=attB2
                     /note="Geneious type: attB2"
     CDS             2283..2999
                     /label=Egfp
     terminator      complement(3190..3414)
                     /label=t35S
     primer_bind     3407..3426
                     /label=35sTerRv
                     /note="/created by=arimura"
     misc_feature    3440..4840
                     /label=Kan_gent
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     Kan_gent (<a 
                     href=""urn:local:.:1w-ag1nupr"">?DEST?pK7WG2DIPS</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     Kan_gent (<a 
                     href=""urn:local:.:1a-aqqnqw8"">【完成】pK7WG2DIPSHSP_platinumO
                     RF125_2.xdna</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     Kan_gent (<a 
                     href=""urn:local:.:19-aqqnqw8"">【完成】pK7WG2DIPSHSP_platinumO
                     RF125_1.xdna</a>)"
                     /note="/Alternative Match=100.00%; 
                     Adjusted-Interval-0:291/-311; Kan_gent (<a 
                     href=""urn:local:.:41-aqqrq9c"">pMDCDIPS-K</a>)"
                     /note="/Alternative Match=100.00%; 
                     Adjusted-Interval-0:291/-311; Kan_gent (<a 
                     href=""urn:local:.:5j-asqci34"">pMDCDIPS-K ORF125-2</a>)"
                     /note="/Alternative Match=100.00%; 
                     Adjusted-Interval-0:291/-311; Kan_gent (<a 
                     href=""urn:local:.:c-9o5wbk3"">pMDCDIPS-K GFP-RFP</a>)"
                     /note="/Alternative Match=100.00%; 
                     Adjusted-Interval-0:291/-311; Kan_gent (<a 
                     href=""urn:local:.:14-at0pjh2"">【完成】pDEST pMDCDIPS-K - 
                     Reaction of 3 fragments Expression clone</a>)"
                     /note="/Alternative Match=100.00%; 
                     Adjusted-Interval-0:291/-311; Kan_gent (<a 
                     href=""urn:local:.:ar-aqqua3y"">pMDCDIPS-K ORF125-1</a>)"
                     /note="/Alternative Match=100.00%; 
                     Adjusted-Interval-0:291/-311; Kan_gent (<a 
                     href=""urn:local:.:3s-aqqq5bj"">【DEST】pDEST 
                     pMDCDIPS-K</a>)"
                     /note="/Primary Match=100.00%; Adjusted-Interval-0:0/0; 
                     Kan_gent (<a 
                     href=""urn:local:.:14-ancw4d4"">pK7WG2DIPS_RPS5Apro 
                     ver2</a>)"
                     /note="/SerialCloner_Arrow=True"
                     /note="/SerialCloner_Color=&h408080"
                     /note="/SerialCloner_Protect=False"
                     /note="/SerialCloner_Show=True"
                     /note="/Transferred From=pK7WG2DIPS_RPS5Apro ver2"
                     /note="/Transferred Similarity=100.00%"
     promoter        3445..3722
                     /label=NOS-P
                     /note="/SerialCloner_Arrow=True"
                     /note="/SerialCloner_Color=&h4682B4"
                     /note="/SerialCloner_Protect=False"
                     /note="/SerialCloner_Show=True"
                     /note="/Transferred From=pMDCDIPS20181026作製途中ほぼ完成？"
                     /note="/Transferred Similarity=99.64%"
                     /note="/X-Transferred From=?DEST?pK7WG2DIPS_RPS5A_cTAL12; 
                     [亘完成]pDESTpK7WG2DIPS_RPS5A_cTAL12; [完成Tandem] 
                     RPS5A-ATP6-2up 20180424.xdna; [間違え完成Tandem] 
                     RPS5A-ATP6-2-1up 20180423.xdna; backup of RPS5A-ATP6-2-1up 
                     20180423.xdna; 【完成】pK7WG2DIPSHSP_platinumORF125_1.xdna 
                     Copy; 【柳瀬間違え】RPS5A_platinum　ATP6-2 ①.xdna"
                     /note="/X-Transferred Similarity=99.64%"
     primer_bind     3732..3756
                     /label=pMDCtoNpt2f
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="/Alternative Match=92.00%; Adjusted-Interval-0:0/0; 
                     pMDCtoNpt2f (<a href=""urn:local:.:5j-asqci34"">pMDCDIPS-K 
                     ORF125-2</a>)"
                     /note="/Alternative Match=92.00%; Adjusted-Interval-0:0/0; 
                     pMDCtoNpt2f (<a href=""urn:local:.:c-9o5wbk3"">pMDCDIPS-K 
                     GFP-RFP</a>)"
                     /note="/Alternative Match=92.00%; Adjusted-Interval-0:0/0; 
                     pMDCtoNpt2f (<a href=""urn:local:.:14-at0pjh2"">【完成】pDEST 
                     pMDCDIPS-K - Reaction of 3 fragments Expression clone</a>)"
                     /note="/Alternative Match=92.00%; Adjusted-Interval-0:0/0; 
                     pMDCtoNpt2f (<a href=""urn:local:.:ar-aqqua3y"">pMDCDIPS-K 
                     ORF125-1</a>)"
                     /note="/Alternative Match=92.00%; Adjusted-Interval-0:0/0; 
                     pMDCtoNpt2f (<a href=""urn:local:.:3s-aqqq5bj"">【DEST】pDEST
                     pMDCDIPS-K</a>)"
                     /note="/Alternative Match=92.00%; Adjusted-Interval-0:0/0; 
                     pMDCtoNpt2f (<a href=""urn:local:.:dj-asqcssr"">【完成】pDEST 
                     pMDCDIPS-K - Reaction of 3 fragments Expression clone</a>)"
                     /note="/Alternative Match=92.00%; Adjusted-Interval-0:0/0; 
                     pMDCtoNpt2f (<a href=""urn:local:.:7p-aqqsmkr"">pMDCDIPS-K 
                     ATP6-2</a>)"
                     /note="/Primary Match=92.00%; Adjusted-Interval-0:0/0; 
                     pMDCtoNpt2f (<a 
                     href=""urn:local:.:41-aqqrq9c"">pMDCDIPS-K</a>)"
                     /note="/Transferred From=pMDCDIPS-K"
                     /note="/Transferred Similarity=92.00%"
                     /note="/created by=arimura"
     misc_feature    3735..4529
                     /label=ORF 1 (frame 2)
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="Geneious type: ORF"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     ORF 1 (frame 2) (<a 
                     href=""urn:local:.:19-aqqnqw8"">【完成】pK7WG2DIPSHSP_platinumO
                     RF125_1.xdna</a>)"
                     /note="/Genetic code=Standard"
                     /note="/Primary Match=100.00%; Adjusted-Interval-0:0/0; ORF
                     1 (frame 2) (<a 
                     href=""urn:local:.:1a-aqqnqw8"">【完成】pK7WG2DIPSHSP_platinumO
                     RF125_2.xdna</a>)"
                     /note="/Reading frame=2"
                     /note="/Transferred 
                     From=【完成】pK7WG2DIPSHSP_platinumORF125_2.xdna"
                     /note="/Transferred Similarity=100.00%"
                     /note="/X-Transferred From=[mitoTAL]RPS5A-Shaorf117-1"
                     /note="/X-Transferred Similarity=100.00%"
                     /note="/created by=ORF Finder"
     primer_bind     complement(3824..3838)
                     /label=pRS-marker
     misc_feature    4844..5176
                     /label=LB_gent
                     /note="/SerialCloner_Arrow=True"
                     /note="/SerialCloner_Color=&h00FFFF; &h84A4C0"
                     /note="/SerialCloner_Protect=False"
                     /note="/SerialCloner_Show=True"
                     /note="/Transferred From=pMDCDIPS20181026作製途中ほぼ完成？"
                     /note="/Transferred Similarity=100.00%"
                     /note="/X-Transferred From=?DEST?pH7WG2DIPS.xdna; 
                     ?DEST?pK7WG2DIPS_RPS5A_cTAL12; 
                     [亘完成]pDESTpK7WG2DIPS_RPS5A_cTAL12; [完成Tandem] 
                     RPS5A-ATP6-2up 20180424.xdna; [間違え完成Tandem] 
                     RPS5A-ATP6-2-1up 20180423.xdna; backup of RPS5A-ATP6-2-1up 
                     20180423.xdna; 【完成】pK7WG2DIPSHSP_platinumORF125_1.xdna 
                     Copy; 【柳瀬間違え】RPS5A_platinum　ATP6-2 ①.xdna"
                     /note="/X-Transferred Similarity=100.00%"
     misc_feature    complement(4914..4938)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     LB T-DNA repeat (<a 
                     href=""urn:local:.:17-b8vpbes"">pDE-Cas9 - pEN-Chimera 
                     DRP3AB1 Expression clone</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     LB T-DNA repeat (<a 
                     href=""urn:local:.:11-b8lef5i"">pDE-Cas9</a>)"
                     /note="/Primary Match=100.00%; Adjusted-Interval-0:0/0; LB 
                     T-DNA repeat (<a 
                     href=""urn:local:.:f-b8voshw"">pDE-Cas9</a>)"
                     /note="/Transferred From=pDE-Cas9"
                     /note="/Transferred Similarity=100.00%"
     misc_feature    5182..6431
                     /label=Spm_gent
                     /note="/SerialCloner_Arrow=True"
                     /note="/SerialCloner_Color=&h00FF80; &h808040"
                     /note="/SerialCloner_Protect=False"
                     /note="/SerialCloner_Show=True"
                     /note="/Transferred From=pMDCDIPS20181026作製途中ほぼ完成？"
                     /note="/Transferred Similarity=100.00%"
                     /note="/X-Transferred From=?DEST?pH7WG2DIPS.xdna; 
                     ?DEST?pK7WG2DIPS_RPS5A_cTAL12; 
                     [亘完成]pDESTpK7WG2DIPS_RPS5A_cTAL12; [完成Tandem] 
                     RPS5A-ATP6-2up 20180424.xdna; [間違え完成Tandem] 
                     RPS5A-ATP6-2-1up 20180423.xdna; backup of RPS5A-ATP6-2-1up 
                     20180423.xdna; 【完成】pK7WG2DIPSHSP_platinumORF125_1.xdna 
                     Copy; 【柳瀬間違え】RPS5A_platinum　ATP6-2 ①.xdna"
                     /note="/X-Transferred Similarity=100.00%"
     misc_feature    5325..6257
                     /label=ORF 1 (frame 2)
                     /note="Geneious type: ORF"
                     /note="/Genetic code=Standard"
                     /note="/Reading frame=2"
                     /note="/Transferred From=pMDCDIPS20181026作製途中ほぼ完成？"
                     /note="/Transferred Similarity=100.00%"
                     /note="/X-Transferred 
                     From=[亘完成]pDESTpK7WG2DIPS_RPS5A_cTAL12; 
                     【完成】pK7WG2DIPSHSP_platinumORF125_1.xdna Copy"
                     /note="/X-Transferred Similarity=100.00%"
                     /note="/created by=ORF Finder"
     CDS             5466..6257
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy,  
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and  
                     streptomycin"
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     SmR (<a href=""urn:local:.:17-b8vpbes"">pDE-Cas9 - 
                     pEN-Chimera DRP3AB1 Expression clone</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     SmR (<a href=""urn:local:.:11-b8lef5i"">pDE-Cas9</a>)"
                     /note="/Primary Match=100.00%; Adjusted-Interval-0:0/0; SmR
                     (<a href=""urn:local:.:f-b8voshw"">pDE-Cas9</a>)"
                     /note="/Transferred From=pDE-Cas9"
                     /note="/Transferred Similarity=100.00%"
                     /note="/Transferred 
                     Translation=MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSD
                     IDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRE
                     LQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEA
                     LNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVI
                     LEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6475..7148
                     /label=pUC origin
                     /note="Geneious type: Origin of replication"
                     /note="/Transferred From=pMDCDIPS20181026作製途中ほぼ完成？, DP1 
                     GFP vector confirmed"
                     /note="/Transferred Similarity=100.00% -> 99.85%"
                     /note="/X-Transferred From=pCRII-TOPO; Virtual DIPS GFP 
                     sakamoto transient plasmid; 
                     [construction予定]L1-L4pENTRATP62upLeft MSH1; 
                     [亘完成]pDESTpK7WG2DIPS_RPS5A_cTAL12; 
                     [反対完成E1]pENTR_L1-L4_ATP621A=cTAL12; 
                     [反対完成E3]pENTR_L3-L2ATP621uprightB.xdna; [完成Tandem] 
                     RPS5A-ATP6-2up 20180424.xdna; 
                     [完成]cTAL12pENTRL1-L2-20180423.xdna; [完成]pENTRMSH1 virtual 
                     SA20180709.xdna; [完成]鈴置L1-L4pENTRATP62upLeft; 
                     [完成]鈴置L3-L2pENTRATP62upRight; [間違え完成Tandem] 
                     RPS5A-ATP6-2-1up 20180423.xdna; pENTR221; 【E1】pENTR L1-L4 
                     cTAL12virtual 20180423.xdna; 【E3】pENTR L3-L2 wakoTAL-R 
                     ATP621upRight.xdna Copy; 
                     【完成】pK7WG2DIPSHSP_platinumORF125_1.xdna Copy; 
                     【柳瀬間違え】RPS5A_platinum　ATP6-2 ①.xdna; 鈴置L1-L4; 鈴置L3-L2"
                     /note="/X-Transferred Similarity=99.85%; 100.00% -> 99.55%"
     rep_origin      6479..7107
                     /direction=RIGHT
                     /label=ColE1 origin
                     /note="/SerialCloner_Arrow=True"
                     /note="/SerialCloner_Color=&h4682B4"
                     /note="/SerialCloner_Protect=False"
                     /note="/SerialCloner_Show=True"
                     /note="/Transferred From=DP1 GFP vector confirmed"
                     /note="/Transferred Similarity=99.84%"
                     /note="/X-Transferred From=?DEST?pH7WG2DIPS.xdna; 
                     ?DEST?pK7WG2DIPS_RPS5A_cTAL12; ?E1?pENTR_L1-L4_; 
                     ?E1?pENTR_L1-L4_wako_136_63-VR-HD.xdna; ?E2?pENTR_R4R3_T; 
                     ?E3?pENTR_L3-L2_wako_136_63-VR-NG.txt.xdna; 
                     ?cTAL1?pENTRL1-L2_assembly_step2?.xdna; Virtual DIPS GFP 
                     sakamoto transient plasmid; 
                     [反対完成E1]pENTR_L1-L4_ATP621A=cTAL12; 
                     [反対完成E3]pENTR_L3-L2ATP621uprightB.xdna; 
                     [完成]cTAL12pENTRL1-L2-20180423.xdna; [完成]pENTRMSH1 virtual 
                     SA20180709.xdna; [完成]鈴置L3-L2pENTRATP62upRight; backup of 
                     RPS5A-ATP6-2-1up 20180423.xdna; pENTR221; 【E3】pENTR L3-L2 
                     wakoTAL-R ATP621upRight.xdna Copy; 
                     【完成】pK7WG2DIPSHSP_platinumORF125_1.xdna Copy; 
                     【柳瀬間違え】RPS5A_platinum　ATP6-2 ①.xdna; 鈴置L1-L4; 鈴置L3-L2"
                     /note="/X-Transferred Similarity=100.00% -> 99.52%"
     misc_feature    6488..7102
                     /gene="rep (pMB1)"
                     /label=rep (pMB1) misc
                     /note="Geneious type: misc"
                     /note="/Transferred From=pMDCDIPS20181026作製途中ほぼ完成？, DP1 
                     GFP vector confirmed"
                     /note="/Transferred Similarity=100.00% -> 99.84%"
                     /note="/X-Transferred From=pSB1C3; Virtual DIPS GFP 
                     sakamoto transient plasmid; 
                     【完成】pK7WG2DIPSHSP_platinumORF125_1.xdna Copy"
                     /note="/X-Transferred Similarity=99.67%; 100.00% -> 99.84%"
     rep_origin      6501..7089
                     /direction=RIGHT
                     /label=rep origin; ori
                     /note="pMB1 origin of replication (clockwise) (RNAII -35 to
                     RNA/DNA switch point); pMB1 origin of replication 
                     (counter-clockwise) (RNAII -35 to RNA/DNA switch point); 
                     pUC19 origin of replication (clockwise) (RNAII -35 to 
                     RNA/DNA switch point); pUC19 origin of replication 
                     (counter-clockwise) (RNAII -35 to RNA/DNA switch point); 
                     high-copy-number ColE1/pMB1/pBR322/pUC origin of  
                     replication"
                     /note="/Transferred From=DP1 GFP vector confirmed, 
                     Exported"
                     /note="/Transferred Similarity=99.83% -> 99.32%"
                     /note="/X-Transferred From=Virtual DIPS GFP sakamoto 
                     transient plasmid"
                     /note="/X-Transferred Similarity=100.00%"
     rep_origin      complement(6501..7089)
                     /direction=LEFT
                     /label=rep origin
                     /note="pMB1 origin of replication (counterclockwise) (RNAII
                     -35 to RNA/DNA switch point)"
                     /note="/Transferred From=DP1 GFP vector confirmed"
                     /note="/Transferred Similarity=99.83%"
                     /note="/X-Transferred From=Virtual DIPS GFP sakamoto 
                     transient plasmid"
                     /note="/X-Transferred Similarity=100.00%"
     misc_RNA        6537..7089
                     /note="RNAII transcript (complementary strand)"
                     /note="/Transferred From=DP1 GFP vector confirmed"
                     /note="/Transferred Similarity=99.82%"
                     /note="/X-Transferred From=Virtual DIPS GFP sakamoto 
                     transient plasmid"
                     /note="/X-Transferred Similarity=100.00%"
     misc_RNA        complement(6540..6647)
                     /label=RNAI transcript
                     /note="RNAI transcript"
                     /note="/Transferred From=DP1 GFP vector confirmed"
                     /note="/Transferred Similarity=100.00%"
                     /note="/X-Transferred From=Virtual DIPS GFP sakamoto 
                     transient plasmid; 【完成】pK7WG2DIPSHSP_platinumORF125_1.xdna 
                     Copy"
                     /note="/X-Transferred Similarity=100.00%"
     primer_bind     6991..7010
                     /label=pBR322ori-F
     primer_bind     7189..7208
                     /label=VR
     primer_bind     7189..7208
                     /label=VR (1)
     primer_bind     7244..7261
                     /label=L4440
     misc_feature    complement(7275..7415)
                     /label=bom
                     /note="basis of mobility region from pBR322"
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     bom (<a href=""urn:local:.:17-b8vpbes"">pDE-Cas9 - 
                     pEN-Chimera DRP3AB1 Expression clone</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     bom (<a href=""urn:local:.:11-b8lef5i"">pDE-Cas9</a>)"
                     /note="/Primary Match=100.00%; Adjusted-Interval-0:0/0; bom
                     (<a href=""urn:local:.:f-b8voshw"">pDE-Cas9</a>)"
                     /note="/Transferred From=pDE-Cas9"
                     /note="/Transferred Similarity=100.00%"
     primer_bind     complement(7342..7361)
                     /label=pRS-marker
     primer_bind     7502..7524
                     /label=pGEX 3'
     rep_origin      complement(7759..7953)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid  
                     pVS1 (Heeb et al., 2000)"
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     pVS1 oriV (<a href=""urn:local:.:17-b8vpbes"">pDE-Cas9 - 
                     pEN-Chimera DRP3AB1 Expression clone</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     pVS1 oriV (<a 
                     href=""urn:local:.:11-b8lef5i"">pDE-Cas9</a>)"
                     /note="/Primary Match=100.00%; Adjusted-Interval-0:0/0; 
                     pVS1 oriV (<a href=""urn:local:.:f-b8voshw"">pDE-Cas9</a>)"
                     /note="/Transferred From=pDE-Cas9"
                     /note="/Transferred Similarity=100.00%"
     misc_feature    complement(8019..9143)
                     /label=ORF 5 (frame 1)
                     /note="Geneious type: ORF"
                     /note="/Genetic code=Standard"
                     /note="/Reading frame=1"
                     /note="/Transferred From=pMDCDIPS20181026作製途中ほぼ完成？"
                     /note="/Transferred Similarity=100.00%"
                     /note="/X-Transferred 
                     From=[亘完成]pDESTpK7WG2DIPS_RPS5A_cTAL12; 
                     【完成】pK7WG2DIPSHSP_platinumORF125_1.xdna Copy"
                     /note="/X-Transferred Similarity=100.00%"
                     /note="/created by=ORF Finder"
     CDS             complement(8019..9119)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="/Adjusted Transferred Similarity=97.00%"
                     /note="/Alternative Match=100.00%; 
                     Adjusted-Interval-0:-33/0 (adjusted to 97.00%; 
                     Adjusted-Interval-0:0/0); pVS1 RepA (<a 
                     href=""urn:local:.:17-b8vpbes"">pDE-Cas9 - pEN-Chimera 
                     DRP3AB1 Expression clone</a>)"
                     /note="/Alternative Match=100.00%; 
                     Adjusted-Interval-0:-33/0 (adjusted to 97.00%; 
                     Adjusted-Interval-0:0/0); pVS1 RepA (<a 
                     href=""urn:local:.:11-b8lef5i"">pDE-Cas9</a>)"
                     /note="/Primary Match=100.00%; Adjusted-Interval-0:-33/0 
                     (adjusted to 97.00%; Adjusted-Interval-0:0/0); pVS1 RepA 
                     (<a href=""urn:local:.:f-b8voshw"">pDE-Cas9</a>)"
                     /note="/Transferred From=pDE-Cas9"
                     /note="/Transferred Similarity=100.00%"
                     /note="/Transferred 
                     Translation=GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQAAA
                     DRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKRDR
                     RAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGRVF
                     RPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEALIS
                     RYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLARR
                     AAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILVMR
                     YRNLIEGEASAGS"
                     /translation="LEPPSPRNRRDGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIE
                     AEARPGESWQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSI
                     MDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNR
                     YREGKGDKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNG
                     RLVTVSEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLID
                     PGIGRFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLK
                     EEAGQAGPILVMRYRNLIEGEASAGS"
     primer_bind     8159..8178
                     /label=3,177 R
     primer_bind     8418..8437
                     /label=2,918 R
     primer_bind     8605..8624
                     /label=2,731 R
     primer_bind     complement(8605..8624)
                     /label=2,712 F
     primer_bind     8606..8625
                     /label=2,730 R
     primer_bind     complement(8606..8625)
                     /label=2,711 F
     primer_bind     complement(8646..8665)
                     /label=2,671 F
     primer_bind     complement(8653..8672)
                     /label=2,664 F
     primer_bind     complement(8655..8674)
                     /label=2,662 F
     primer_bind     complement(8657..8676)
                     /label=2,660 F
     primer_bind     complement(8930..8949)
                     /label=2,387 F
     misc_feature    9300..9311
                     /label=helix
                     /note="Geneious type: helix"
                     /note="/Transferred From=pMDCDIPS20181026作製途中ほぼ完成？"
                     /note="/Transferred Similarity=100.00%"
                     /note="/X-Transferred From=NARH"
                     /note="/X-Transferred Translation Similarity=100.00%"
                     /note="/sec_str_type=helix"
     CDS             complement(9520..10149)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid  
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     pVS1 StaA (<a href=""urn:local:.:17-b8vpbes"">pDE-Cas9 - 
                     pEN-Chimera DRP3AB1 Expression clone</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     pVS1 StaA (<a 
                     href=""urn:local:.:11-b8lef5i"">pDE-Cas9</a>)"
                     /note="/Primary Match=100.00%; Adjusted-Interval-0:0/0; 
                     pVS1 StaA (<a href=""urn:local:.:f-b8voshw"">pDE-Cas9</a>)"
                     /note="/Transferred From=pDE-Cas9"
                     /note="/Transferred Similarity=100.00%"
                     /note="/Transferred 
                     Translation=MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSARDW
                     AAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIPVQ
                     PSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPILE
                     SRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    complement(10846..10857)
                     /label=helix
                     /note="Geneious type: helix"
                     /note="/Transferred From=pMDCDIPS20181026作製途中ほぼ完成？"
                     /note="/Transferred Similarity=100.00%"
                     /note="/X-Transferred From=NARH"
                     /note="/X-Transferred Translation Similarity=100.00%"
                     /note="/sec_str_type=helix"
     misc_feature    11335..11534
                     /label=RB
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="Geneious type: motif"
                     /note="/Primary Match=100.00%; Adjusted-Interval-0:0/0; RB 
                     (<a 
                     href=""urn:local:.:j-9r0ru3x"">pH7WG2DIPS_ORF79cTAL8.xdna 
                     Copy</a>)"
                     /note="/Transferred From=pH7WG2DIPS_ORF79cTAL8.xdna Copy"
                     /note="/Transferred Similarity=100.00%"
                     /note="/created by=arimura"
                     /note="/modified by=arimura"
     misc_feature    11335..11534
                     /label=RB_gent
                     /note="/SerialCloner_Arrow=True"
                     /note="/SerialCloner_Color=&h80FFFF; &h84A4C0"
                     /note="/SerialCloner_Protect=False"
                     /note="/SerialCloner_Show=True"
                     /note="/Transferred From=pMDCDIPS20181026作製途中ほぼ完成？"
                     /note="/Transferred Similarity=100.00%"
                     /note="/X-Transferred From=?DEST?pH7WG2DIPS.xdna; 
                     ?DEST?pK7WG2DIPS_RPS5A_cTAL12; 
                     [亘完成]pDESTpK7WG2DIPS_RPS5A_cTAL12; [完成Tandem] 
                     RPS5A-ATP6-2up 20180424.xdna; [間違え完成Tandem] 
                     RPS5A-ATP6-2-1up 20180423.xdna; backup of RPS5A-ATP6-2-1up 
                     20180423.xdna; 【完成】pK7WG2DIPSHSP_platinumORF125_1.xdna 
                     Copy; 【柳瀬間違え】RPS5A_platinum　ATP6-2 ①.xdna"
                     /note="/X-Transferred Similarity=100.00%"
     misc_feature    complement(11449..11473)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     RB T-DNA repeat (<a 
                     href=""urn:local:.:17-b8vpbes"">pDE-Cas9 - pEN-Chimera 
                     DRP3AB1 Expression clone</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     RB T-DNA repeat (<a 
                     href=""urn:local:.:11-b8lef5i"">pDE-Cas9</a>)"
                     /note="/Primary Match=100.00%; Adjusted-Interval-0:0/0; RB 
                     T-DNA repeat (<a 
                     href=""urn:local:.:f-b8voshw"">pDE-Cas9</a>)"
                     /note="/Transferred From=pDE-Cas9"
                     /note="/Transferred Similarity=100.00%"
     primer_bind     11502..11532
                     /label=GentRBcheck1F
     misc_feature    11535..13194
                     /label=RPS5Apro
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     RPS5Apro (<a 
                     href=""urn:local:.:g3-asqd2hr"">【完成】pK7WG2DIPS_RPS5Apro - 
                     Reaction of 3 fragments Expression clone</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     RPS5Apro (<a 
                     href=""urn:local:.:1w-ag1nupr"">?DEST?pK7WG2DIPS</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     RPS5Apro (<a 
                     href=""urn:local:.:2d-a6x9885"">pH7WG2DIPS_RPS5Apro 
                     ver2</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     RPS5Apro (<a 
                     href=""urn:local:.:2h-a6x9gky"">【E2】pENTR_R4R3_T-HSP-P-RPS5
                     A_DIPSsig ver2</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     RPS5Apro (<a 
                     href=""urn:local:.:kt-asqdgru"">pH7WG2DIPS_RPS5Apro ver2 - 
                     Reaction of 3 fragments Expression clone</a>)"
                     /note="/Primary Match=100.00%; Adjusted-Interval-0:0/0; 
                     RPS5Apro (<a 
                     href=""urn:local:.:14-ancw4d4"">pK7WG2DIPS_RPS5Apro 
                     ver2</a>)"
                     /note="/SerialCloner_Arrow=True"
                     /note="/SerialCloner_Color=&hFF8000"
                     /note="/SerialCloner_Protect=False"
                     /note="/SerialCloner_Show=True"
                     /note="/Transferred From=pK7WG2DIPS_RPS5Apro ver2"
                     /note="/Transferred Similarity=100.00%"
     primer_bind     11545..11565
                     /label=RPS5AproFw
                     /note="/created by=arimura"
     misc_feature    13213..13377
                     /label=Mt DIPS
                     /note="/SerialCloner_Arrow=True"
                     /note="/SerialCloner_Color=&h0000A0; &h84A4C0"
                     /note="/SerialCloner_Protect=False"
                     /note="/SerialCloner_Show=True"
                     /note="/Transferred From=DP1 GFP vector confirmed, 
                     pMDCDIPS20181026作製途中ほぼ完成？"
                     /note="/Transferred Similarity=100.00%"
                     /note="/X-Transferred From=?DEST?pH7WG2DIPS.xdna; Virtual 
                     DIPS GFP sakamoto transient plasmid; [完成Tandem] 
                     RPS5A-ATP6-2up 20180424.xdna; [間違え完成Tandem] 
                     RPS5A-ATP6-2-1up 20180423.xdna; backup of RPS5A-ATP6-2-1up 
                     20180423.xdna; 【完成】pK7WG2DIPSHSP_platinumORF125_1.xdna 
                     Copy; 【柳瀬間違え】RPS5A_platinum　ATP6-2 ①.xdna"
                     /note="/X-Transferred Similarity=100.00%"
     misc_feature    13270..13378
                     /label=ORF 4 (frame 1)
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="Geneious type: ORF"
                     /note="/Alternative Match=99.08%; Adjusted-Interval-0:0/0; 
                     ORF 4 (frame 1) (<a 
                     href=""urn:local:.:14-at0pjh2"">【完成】pDEST pMDCDIPS-K - 
                     Reaction of 3 fragments Expression clone</a>)"
                     /note="/Alternative Match=99.08%; Adjusted-Interval-0:0/0; 
                     ORF 4 (frame 1) (<a 
                     href=""urn:local:.:dj-asqcssr"">【完成】pDEST pMDCDIPS-K - 
                     Reaction of 3 fragments Expression clone</a>)"
                     /note="/Genetic code=Standard"
                     /note="/Primary Match=99.08%; Adjusted-Interval-0:0/0; ORF 
                     4 (frame 1) (<a 
                     href=""urn:local:.:3h-asqc4fa"">【E2】pENTR_R4R3_T-P-MDC_DIPS
                     </a>)"
                     /note="/Reading frame=1"
                     /note="/Transferred From=【E2】pENTR_R4R3_T-P-MDC_DIPS"
                     /note="/Transferred Similarity=99.08%"
                     /note="/created by=ORF Finder"
     misc_feature    13270..13377
                     /label=Mt DIPS
                     /note="/SerialCloner_Arrow=True"
                     /note="/SerialCloner_Color=&h84A4C0"
                     /note="/SerialCloner_Protect=False"
                     /note="/SerialCloner_Show=True"
                     /note="/Transferred From=DP1 GFP vector confirmed, 
                     pMDCDIPS20181026作製途中ほぼ完成？"
                     /note="/Transferred Similarity=100.00%"
                     /note="/X-Transferred From=?DEST?pK7WG2DIPS_RPS5A_cTAL12; 
                     ?E2?pENTR_R4R3_T; Virtual DIPS GFP sakamoto transient 
                     plasmid; [亘完成]pDESTpK7WG2DIPS_RPS5A_cTAL12; [完成Tandem] 
                     RPS5A-ATP6-2up 20180424.xdna; [間違え完成Tandem] 
                     RPS5A-ATP6-2-1up 20180423.xdna; 
                     【完成】pK7WG2DIPSHSP_platinumORF125_1.xdna Copy; 
                     【柳瀬間違え】RPS5A_platinum　ATP6-2 ①.xdna"
                     /note="/X-Transferred Similarity=100.00%"
     protein_bind    13390..13407
                     /gene="mutant version of attR"
                     /label=attR1; attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /note="/Transferred From=Exported, Exported, 
                     pMDCDIPS20181026作製途中ほぼ完成？"
                     /note="/Transferred Similarity=100.00% -> 97.60%"
     misc_feature    13391..13407
                     /label=attB1
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="Geneious type: attB1"
                     /note="/Alternative Match=95.24%; Adjusted-Interval-0:0/0; 
                     attB1 (<a href=""urn:local:.:25-aqqp44t"">pMDC7 
                     GFP-RFP</a>)"
                     /note="/Alternative Match=95.24%; Adjusted-Interval-0:0/0; 
                     attB1 (<a 
                     href=""urn:local:.:2a-aqqp44u"">pMDC7_TAL_ATP6-2up</a>)"
                     /note="/Alternative Match=95.24%; Adjusted-Interval-0:0/0; 
                     attB1 (<a 
                     href=""urn:local:.:2a-aqqp44u"">pMDC7_TAL_ATP6-2up</a>)"
                     /note="/Alternative Match=95.24%; Adjusted-Interval-0:0/0; 
                     attB1 (<a 
                     href=""urn:local:.:g3-asqd2hr"">【完成】pK7WG2DIPS_RPS5Apro - 
                     Reaction of 3 fragments Expression clone</a>)"
                     /note="/Alternative Match=95.24%; Adjusted-Interval-0:0/0; 
                     attB1 (<a 
                     href=""urn:local:.:3f-aqqpt4w"">pMDC7_TAL_ATP6-2up</a>)"
                     /note="/Alternative Match=95.24%; Adjusted-Interval-0:0/0; 
                     attB1 (<a 
                     href=""urn:local:.:3f-aqqpt4w"">pMDC7_TAL_ATP6-2up</a>)"
                     /note="/Alternative Match=95.24%; Adjusted-Interval-0:0/0; 
                     attB1 (<a href=""urn:local:.:5j-asqci34"">pMDCDIPS-K 
                     ORF125-2</a>)"
                     /note="/Alternative Match=95.24%; Adjusted-Interval-0:0/0; 
                     attB1 (<a href=""urn:local:.:5j-asqci34"">pMDCDIPS-K 
                     ORF125-2</a>)"
                     /note="/Alternative Match=95.24%; Adjusted-Interval-0:0/0; 
                     attB1 (<a href=""urn:local:.:17-b8vpbes"">pDE-Cas9 - 
                     pEN-Chimera DRP3AB1 Expression clone</a>)"
                     /note="/Primary Match=95.24%; Adjusted-Interval-0:0/0; 
                     attB1 (<a href=""urn:local:.:25-aqqp44t"">pMDC7 
                     GFP-RFP</a>)"
                     /note="/Transferred From=pMDC7 GFP-RFP"
                     /note="/Transferred Similarity=95.24%"
                     /note="/X-Transferred From=[mitoTAL]RPS5A-Shaorf117-1"
                     /note="/X-Transferred Similarity=100.00%"
     misc_feature    13391..13407
                     /label=attR3
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="Geneious type: attR3"
                     /note="/Alternative Match=96.00%; Adjusted-Interval-0:0/0; 
                     attR3 (<a 
                     href=""urn:local:.:3h-asqc4fa"">【E2】pENTR_R4R3_T-P-MDC_DIPS
                     </a>)"
                     /note="/Alternative Match=96.00%; Adjusted-Interval-0:0/0; 
                     attR3 (<a 
                     href=""urn:local:.:2h-a6x9gky"">【E2】pENTR_R4R3_T-HSP-P-RPS5
                     A_DIPSsig ver2</a>)"
                     /note="/Primary Match=96.00%; Adjusted-Interval-0:0/0; 
                     attR3 (<a 
                     href=""urn:local:.:m-a50mww7"">【E2】pENTR_R4R3_T-HSP-P-RPS5A
                     _DIPSsig - コピー.xdna</a>)"
                     /note="/Transferred 
                     From=【E2】pENTR_R4R3_T-HSP-P-RPS5A_DIPSsig - コピー.xdna"
                     /note="/Transferred Similarity=96.00%"
     misc_feature    13391..13407
                     /label=attR4
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="Geneious type: attR4"
                     /note="/Alternative Match=96.00%; Adjusted-Interval-0:0/0; 
                     attR4 (<a 
                     href=""urn:local:.:3h-asqc4fa"">【E2】pENTR_R4R3_T-P-MDC_DIPS
                     </a>)"
                     /note="/Alternative Match=96.00%; Adjusted-Interval-0:0/0; 
                     attR4 (<a 
                     href=""urn:local:.:2h-a6x9gky"">【E2】pENTR_R4R3_T-HSP-P-RPS5
                     A_DIPSsig ver2</a>)"
                     /note="/Primary Match=96.00%; Adjusted-Interval-0:0/0; 
                     attR4 (<a 
                     href=""urn:local:.:m-a50mww7"">【E2】pENTR_R4R3_T-HSP-P-RPS5A
                     _DIPSsig - コピー.xdna</a>)"
                     /note="/Transferred 
                     From=【E2】pENTR_R4R3_T-HSP-P-RPS5A_DIPSsig - コピー.xdna"
                     /note="/Transferred Similarity=96.00%"
     misc_feature    13391..13407
                     /label=attR1
                     /note="Geneious type: attR1"
     misc_feature    13391..13407
                     /label=attR1
                     /note="Geneious type: attR1"
     misc_feature    13391..13407
                     /label=attR2
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="Geneious type: attR2"
                     /note="/Alternative Match=97.60%; Adjusted-Interval-0:0/0; 
                     attR2 (<a href=""urn:local:.:f-b8voshw"">pDE-Cas9</a>)"
                     /note="/Alternative Match=97.60%; Adjusted-Interval-0:0/0; 
                     attR2 (<a 
                     href=""urn:local:.:14-ancw4d4"">pK7WG2DIPS_RPS5Apro 
                     ver2</a>)"
                     /note="/Alternative Match=97.60%; Adjusted-Interval-0:0/0; 
                     attR2 (<a 
                     href=""urn:local:.:2d-a6x9885"">pH7WG2DIPS_RPS5Apro 
                     ver2</a>)"
                     /note="/Alternative Match=97.60%; Adjusted-Interval-0:0/0; 
                     attR2 (<a href=""urn:local:.:3s-aqqq5bj"">【DEST】pDEST 
                     pMDCDIPS-K</a>)"
                     /note="/Primary Match=97.60%; Adjusted-Interval-0:0/0; 
                     attR2 (<a 
                     href=""urn:local:.:e-a429oh8"">【DEST】pK7WG2DIPS_RPS5Apro - 
                     コピー.xdna</a>)"
                     /note="/Transferred From=【DEST】pK7WG2DIPS_RPS5Apro - 
                     コピー.xdna"
                     /note="/Transferred Similarity=97.60%"
     misc_recomb     13391..13407
                     /label=AttR1
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     AttR1 (<a 
                     href=""urn:local:.:1w-ag1nupr"">?DEST?pK7WG2DIPS</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     AttR1 (<a 
                     href=""urn:local:.:2d-a6x9885"">pH7WG2DIPS_RPS5Apro 
                     ver2</a>)"
                     /note="/Alternative Match=100.00%; 
                     Adjusted-Interval-0:0/-113; AttR1 (<a 
                     href=""urn:local:.:kt-asqdgru"">pH7WG2DIPS_RPS5Apro ver2 - 
                     Reaction of 3 fragments Expression clone</a>)"
                     /note="/Alternative Match=97.58%; Adjusted-Interval-0:0/0; 
                     AttR2 (<a 
                     href=""urn:local:.:14-ancw4d4"">pK7WG2DIPS_RPS5Apro 
                     ver2</a>)"
                     /note="/Alternative Match=97.58%; Adjusted-Interval-0:0/0; 
                     AttR2 (<a 
                     href=""urn:local:.:1w-ag1nupr"">?DEST?pK7WG2DIPS</a>)"
                     /note="/Alternative Match=97.58%; Adjusted-Interval-0:0/0; 
                     AttR2 (<a 
                     href=""urn:local:.:2d-a6x9885"">pH7WG2DIPS_RPS5Apro 
                     ver2</a>)"
                     /note="/Alternative Match=96.77%; Adjusted-Interval-0:0/0; 
                     attR3 (<a 
                     href=""urn:local:.:3h-asqc4fa"">【E2】pENTR_R4R3_T-P-MDC_DIPS
                     </a>)"
                     /note="/Alternative Match=96.77%; Adjusted-Interval-0:0/0; 
                     attR3 (<a 
                     href=""urn:local:.:2h-a6x9gky"">【E2】pENTR_R4R3_T-HSP-P-RPS5
                     A_DIPSsig ver2</a>)"
                     /note="/Alternative Match=96.77%; Adjusted-Interval-0:0/0; 
                     attR3 (<a 
                     href=""urn:local:.:j-b1wtsla"">?E2?pENTR_R4R3_T-HSP-P-35S_D
                     IPSsig????????.xdna</a>)"
                     /note="/Primary Match=100.00%; Adjusted-Interval-0:0/0; 
                     AttR1 (<a 
                     href=""urn:local:.:14-ancw4d4"">pK7WG2DIPS_RPS5Apro 
                     ver2</a>)"
                     /note="/SerialCloner_Arrow=True"
                     /note="/SerialCloner_Color=&hDDA0DD"
                     /note="/SerialCloner_Protect=False"
                     /note="/SerialCloner_Show=True"
                     /note="/Transferred From=pK7WG2DIPS_RPS5Apro ver2"
                     /note="/Transferred Similarity=100.00%"
     protein_bind    13391..13407
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /note="/Transferred From=pMDCDIPS20181026作製途中ほぼ完成？"
                     /note="/Transferred Similarity=100.00%"
     misc_feature    complement(13391..13407)
                     /label=attR4
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="/Primary Match=96.77%; Adjusted-Interval-0:0/0; 
                     attR4 (<a 
                     href=""urn:local:.:j-b1wtsla"">?E2?pENTR_R4R3_T-HSP-P-35S_D
                     IPSsig????????.xdna</a>)"
                     /note="/SerialCloner_Arrow=True"
                     /note="/SerialCloner_Color=&hBD3DC0"
                     /note="/SerialCloner_Protect=True"
                     /note="/SerialCloner_Show=True"
                     /note="/Transferred 
                     From=?E2?pENTR_R4R3_T-HSP-P-35S_DIPSsig????????.xdna"
                     /note="/Transferred Similarity=96.77%"
     misc_feature    13391..13407
                     /label=attR3
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="/Primary Match=96.75%; Adjusted-Interval-0:0/0; 
                     attR3 (<a 
                     href=""urn:local:.:j-b1wtsla"">?E2?pENTR_R4R3_T-HSP-P-35S_D
                     IPSsig????????.xdna</a>)"
                     /note="/SerialCloner_Arrow=True"
                     /note="/SerialCloner_Color=&hC02E73"
                     /note="/SerialCloner_Protect=True"
                     /note="/SerialCloner_Show=True"
                     /note="/Transferred 
                     From=?E2?pENTR_R4R3_T-HSP-P-35S_DIPSsig????????.xdna"
                     /note="/Transferred Similarity=96.75%"
     misc_feature    complement(13408..13414)
                     /label=AttB1
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="/Alternative Match=92.00%; Adjusted-Interval-0:0/0; 
                     AttB1 (<a 
                     href=""urn:local:.:33-b4vvpao"">【完成】pK7WG2DIPSHSP_platinumO
                     RF125_2.xdna</a>)"
                     /note="/Alternative Match=92.00%; Adjusted-Interval-0:0/0; 
                     AttB1 (<a 
                     href=""urn:local:.:2r-az6q1lw"">[mitoTAL]RPS5A-Shaorf117-1<
                     /a>)"
                     /note="/Alternative Match=92.00%; Adjusted-Interval-0:0/0; 
                     AttB1 (<a 
                     href=""urn:local:.:p-b4qttuj"">【完成】pK7WG2DIPSHSP_platinumSm
                     elORF218-2 完成20191031.xdna</a>)"
                     /note="/Alternative Match=92.00%; Adjusted-Interval-0:0/0; 
                     AttB1 (<a 
                     href=""urn:local:.:2d-b5139h4"">【完成】pK7WG2DIPSHSP_platinumS
                     melORF218-1完成20191031.xdna</a>)"
                     /note="/Alternative Match=92.00%; Adjusted-Interval-0:0/0; 
                     AttB1 (<a href=""urn:local:.:f-c6lzzo2"">【完成】pK7WG2DIPS 
                     SmelORF218-2'</a>)"
                     /note="/Alternative Match=92.00%; Adjusted-Interval-0:0/0; 
                     AttB1 (<a 
                     href=""urn:local:.:32-b4vvpao"">【完成】pK7WG2DIPSHSP_platinumO
                     RF125_1.xdna</a>)"
                     /note="/Alternative Match=92.00%; Adjusted-Interval-0:0/0; 
                     AttB1 (<a 
                     href=""urn:local:.:2s-az6q1ly"">[mitoTAL]RPS5A-Shaorf117-2<
                     /a>)"
                     /note="/Alternative Match=92.00%; Adjusted-Interval-0:0/0; 
                     AttB1 (<a 
                     href=""urn:local:.:f-b221x1j"">【完成】pK7WG2DIPSHSP_GFPtagRFP.
                     xdna</a>)"
                     /note="/Alternative Match=92.00%; Adjusted-Interval-0:0/0; 
                     AttB1 (<a href=""urn:local:.:h-c5s8qqf"">【完成】pK7WG2DIPS 
                     SmelORF218-1'</a>)"
                     /note="/Primary Match=92.00%; Adjusted-Interval-0:0/0; 
                     AttB1 (<a 
                     href=""urn:local:.:2t-az6q1lz"">[mitoTAL]RPS5A-Shaorf122</a
                     >)"
                     /note="/SerialCloner_Arrow=True"
                     /note="/SerialCloner_Color=&hDDA0DD"
                     /note="/SerialCloner_Protect=False"
                     /note="/SerialCloner_Show=True"
                     /note="/Transferred From=[mitoTAL]RPS5A-Shaorf122"
                     /note="/Transferred Similarity=92.00%"
                     /note="/X-Transferred From=[完成Tandem] RPS5A-ATP6-2up 
                     20180424.xdna, [完成ver2] RPS5A-ATP6-2①(up) 20190406.xdna"
                     /note="/X-Transferred Similarity=100.00%"
     misc_feature    13408..13411
                     /label=attL1
                     /note="Geneious type: attL1"
     misc_recomb     13408..13411
                     /label=AttL1
                     /note="/SerialCloner_Arrow=True"
                     /note="/SerialCloner_Color=&hDDA0DD"
                     /note="/SerialCloner_Protect=False"
                     /note="/SerialCloner_Show=True"
     misc_feature    complement(13417..13443)
                     /label=HA tag
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="Geneious type: Tag"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     HA tag (<a 
                     href=""urn:local:.:p-b4qttuj"">【完成】pK7WG2DIPSHSP_platinumSm
                     elORF218-2 完成20191031.xdna</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     HA tag (<a 
                     href=""urn:local:.:2d-b5139h4"">【完成】pK7WG2DIPSHSP_platinumS
                     melORF218-1完成20191031.xdna</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     HA tag (<a 
                     href=""urn:local:.:32-b4vvpao"">【完成】pK7WG2DIPSHSP_platinumO
                     RF125_1.xdna</a>)"
                     /note="/Imported from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Plas
                     Mapper</a>"
                     /note="/Primary Match=100.00%; Adjusted-Interval-0:0/0; HA 
                     tag (<a 
                     href=""urn:local:.:33-b4vvpao"">【完成】pK7WG2DIPSHSP_platinumO
                     RF125_2.xdna</a>)"
                     /note="/Transferred 
                     From=【完成】pK7WG2DIPSHSP_platinumORF125_2.xdna"
                     /note="/Transferred Similarity=100.00%"
                     /note="/X-Transferred From=190404-4L1L4_M13fw-PREMIX.ab1"
                     /note="/X-Transferred Similarity=100.00%"
     CDS             13417..13443
                     /label=HA tag
                     /note="/SerialCloner_Arrow=True"
                     /note="/SerialCloner_Color=&h008000"
                     /note="/SerialCloner_Protect=False"
                     /note="/SerialCloner_Show=True"
     primer_bind     complement(13417..13441)
                     /label=HAcheck1R
     misc_feature    13450..13857
                     /label=N-term region_wako; N-term region_wako compact;...
                     /note="/SerialCloner_Arrow=True"
                     /note="/SerialCloner_Color=&h804000"
                     /note="/SerialCloner_Protect=False"
                     /note="/SerialCloner_Show=True"
                     /note="/Transferred_From=【E1】pENTR_L1-L4_NG_G1333-DddtoxA-C
                     , 【E1】pENTR_L1-L4_NG_G1333-DddtoxA-N, 
                     【E1】pENTR_L1-L4_NG_G1397-DddtoxA-C, 
                     【E1】pENTR_L1-L4_NG_G1397-DddtoxA-N, 
                     【E1】pENTR_L1-L4_NI_G1333-DddtoxA-C, 
                     【E1】pENTR_L1-L4_NI_G1333-DddtoxA-N, 
                     【E1】pENTR_L1-L4_NI_G1397-DddtoxA-C, 
                     【E1】pENTR_L1-L4_NI_G1397-DddtoxA-N, 
                     【E1】pENTR_L1-L4_NN_G1333-DddtoxA-C, 
                     【E1】pENTR_L1-L4_NN_G1333-DddtoxA-N, 
                     【E1】pENTR_L1-L4_NN_G1397-DddtoxA-C, 
                     【E1】pENTR_L1-L4_NN_G1397-DddtoxA-N, 
                     pK7WG2DIPSHSP_ORF125_1.xdna, pK7WG2DIPSHSP_ORF125_2.xdna, 
                     pH7WG2DIPS_ORF79cTAL14.xdna, pH7WG2DIPS_ORF79cTAL8.xdna"
                     /note="/Transferred_Similarity=100.00% -> 99.26%"
                     /note="/X-Transferred_From=pH7WG2DIPS_ORF79cTAL14.xdna; 
                     pH7WG2DIPS_ORF79cTAL8.xdna; pK7WG2DIPSHSP_ORF125_1.xdna; 
                     pK7WG2DIPSHSP_ORF125_2.xdna; 
                     【E1】pENTR_L1-L4_NI_G1333-DddtoxA-C; 
                     【E1】pENTR_L1-L4_NI_G1333-DddtoxA-N; 
                     【E1】pENTR_L1-L4_NI_G1397-DddtoxA-C; 
                     【E1】pENTR_L1-L4_NI_G1397-DddtoxA-N; 
                     【E1】pENTR_L1-L4_NN_G1333-DddtoxA-C; 
                     【E1】pENTR_L1-L4_NN_G1333-DddtoxA-N; 
                     【E1】pENTR_L1-L4_NN_G1397-DddtoxA-C; 
                     【E1】pENTR_L1-L4_NN_G1397-DddtoxA-N"
                     /note="/X-Transferred_Similarity=100.00% -> 99.26%; 99.75% 
                     -> 99.26%"
     primer_bind     13670..13689
                     /label=TALF1r
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     TALF1r (<a 
                     href=""urn:local:.:2t-az6q1lz"">[mitoTAL]RPS5A-Shaorf122</a
                     >)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     TALF1r (<a 
                     href=""urn:local:.:2r-az6q1lw"">[mitoTAL]RPS5A-Shaorf117-1<
                     /a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     TALF1r (<a 
                     href=""urn:local:.:2r-az6q1lw"">[mitoTAL]RPS5A-Shaorf117-1<
                     /a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     TALF1r (<a 
                     href=""urn:local:.:p-b4qttuj"">【完成】pK7WG2DIPSHSP_platinumSm
                     elORF218-2 完成20191031.xdna</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     TALF1r (<a 
                     href=""urn:local:.:p-b4qttuj"">【完成】pK7WG2DIPSHSP_platinumSm
                     elORF218-2 完成20191031.xdna</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     TALF1r (<a 
                     href=""urn:local:.:2d-b5139h4"">【完成】pK7WG2DIPSHSP_platinumS
                     melORF218-1完成20191031.xdna</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     TALF1r (<a 
                     href=""urn:local:.:2d-b5139h4"">【完成】pK7WG2DIPSHSP_platinumS
                     melORF218-1完成20191031.xdna</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     TALF1r (<a href=""urn:local:.:f-c6lzzo2"">【完成】pK7WG2DIPS 
                     SmelORF218-2'</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     TALF1r (<a href=""urn:local:.:f-c6lzzo2"">【完成】pK7WG2DIPS 
                     SmelORF218-2'</a>)"
                     /note="/Primary Match=100.00%; Adjusted-Interval-0:0/0; 
                     TALF1r (<a 
                     href=""urn:local:.:2t-az6q1lz"">[mitoTAL]RPS5A-Shaorf122</a
                     >)"
                     /note="/Transferred From=[mitoTAL]RPS5A-Shaorf122"
                     /note="/Transferred Similarity=100.00%"
                     /note="/X-Transferred From=[mitoTAL]RPS5A-Shaorf117-1"
                     /note="/X-Transferred Similarity=100.00%"
                     /note="/created by=arimura"
     primer_bind     13670..13689
                     /label=TALF1
     misc_feature    15853..16012
                     /label=ORF 5 (frame 3)
                     /note="Geneious type: ORF"
                     /note="/Genetic_code=Standard"
                     /note="/Reading_frame=3"
                     /note="/created_by=ORF Finder"
     misc_feature    15853..15975
                     /label=C-term region_Mok2020
                     /note="/SerialCloner_Arrow=True"
                     /note="/SerialCloner_Color=&h804000"
                     /note="/SerialCloner_Protect=False"
                     /note="/SerialCloner_Show=True"
                     /note="/modified_by=Issei.N"
     primer_bind     15902..15921
                     /label=TALR1r
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     TALR1r (<a 
                     href=""urn:local:.:2t-az6q1lz"">[mitoTAL]RPS5A-Shaorf122</a
                     >)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     TALR1r (<a 
                     href=""urn:local:.:2r-az6q1lw"">[mitoTAL]RPS5A-Shaorf117-1<
                     /a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     TALR1r (<a 
                     href=""urn:local:.:2r-az6q1lw"">[mitoTAL]RPS5A-Shaorf117-1<
                     /a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     TALR1r (<a 
                     href=""urn:local:.:p-b4qttuj"">【完成】pK7WG2DIPSHSP_platinumSm
                     elORF218-2 完成20191031.xdna</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     TALR1r (<a 
                     href=""urn:local:.:p-b4qttuj"">【完成】pK7WG2DIPSHSP_platinumSm
                     elORF218-2 完成20191031.xdna</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     TALR1r (<a 
                     href=""urn:local:.:2d-b5139h4"">【完成】pK7WG2DIPSHSP_platinumS
                     melORF218-1完成20191031.xdna</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     TALR1r (<a 
                     href=""urn:local:.:2d-b5139h4"">【完成】pK7WG2DIPSHSP_platinumS
                     melORF218-1完成20191031.xdna</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     TALR1r (<a href=""urn:local:.:f-c6lzzo2"">【完成】pK7WG2DIPS 
                     SmelORF218-2'</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     TALR1r (<a href=""urn:local:.:f-c6lzzo2"">【完成】pK7WG2DIPS 
                     SmelORF218-2'</a>)"
                     /note="/Primary Match=100.00%; Adjusted-Interval-0:0/0; 
                     TALR1r (<a 
                     href=""urn:local:.:2t-az6q1lz"">[mitoTAL]RPS5A-Shaorf122</a
                     >)"
                     /note="/Transferred From=[mitoTAL]RPS5A-Shaorf122"
                     /note="/Transferred Similarity=100.00%"
                     /note="/X-Transferred From=[mitoTAL]RPS5A-Shaorf117-1"
                     /note="/X-Transferred Similarity=100.00%"
                     /note="/created by=arimura"
     primer_bind     complement(15902..15921)
                     /label=TALR2
     primer_bind     15976..15995
                     /label=G1397-N_Fw
     misc_feature    15976..15981
                     /label=2aa linker
                     /note="/created_by=Issei.N"
                     /note="/modified_by=Issei.N"
     misc_feature    15982..16012
                     /label=G1397-DddtoxA-N
                     /note="/created_by=Issei.N"
                     /note="/modified_by=Issei.N"
     misc_feature    16015..16500
                     /label=TadA
                     /note="Geneious type: motif"
                     /note="/created by=arimura"
     misc_feature    16525..16939
                     /label=DddtoxA-E1347A
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="/Alternative Match=79.94%; Adjusted-Interval-0:0/0; 
                     G1397-DddtoxA-N (<a 
                     href=""urn:local:.:f-e93co8d"">【完成】pK7WG2NLSHSP_platinum_L/
                     R:1397C/N_AtDRP3AG2TtoM</a>)"
                     /note="/Alternative Match=79.94%; Adjusted-Interval-0:0/0; 
                     G1397-DddtoxA-N (<a 
                     href=""urn:local:.:hl-dm2luks"">pKRPS5A-DIPS-1397N/C 
                     nTALECDchmFYE OleG</a>)"
                     /note="/Alternative Match=79.94%; Adjusted-Interval-0:0/0; 
                     G1397-DddtoxA-N (<a 
                     href=""urn:local:.:1s-e7n3br9"">【完成】pK7WG2NLSHSP_platinum_L
                     /R:1397N/C_CYO1_ex1</a>)"
                     /note="/Alternative Match=79.94%; Adjusted-Interval-0:0/0; 
                     G1397-DddtoxA-N (<a 
                     href=""urn:local:.:h8-dm2lak3"">[有村完成ver2]pKRPS5A-DIPS-1397
                     N/C ATP6-2WY OleG Copy 2</a>)"
                     /note="/Alternative Match=79.94%; Adjusted-Interval-0:0/0; 
                     G1397-DddtoxA-N (<a 
                     href=""urn:local:.:s-e93cp11"">【完成】pK7WG2NLSHSP_platinum_L/
                     R:1397C/N_nTALECD-TUB4LHT178I</a>)"
                     /note="/Alternative Match=79.94%; Adjusted-Interval-0:0/0; 
                     G1397-DddtoxA-N (<a 
                     href=""urn:local:.:16-e9d8kyw"">【完成】pK7WG2NLSHSP_platinum_L
                     /R:1397C/N_nTALECD-TUB4RHG96D</a>)"
                     /note="/Alternative Match=79.94%; Adjusted-Interval-0:0/0; 
                     G1397-DddtoxA-N (<a 
                     href=""urn:local:.:n-e93coze"">【完成】pK7WG2NLSHSP_platinum_L/
                     R:1397C/N_nTALECD-TUB4RHG96D</a>)"
                     /note="/Alternative Match=79.94%; Adjusted-Interval-0:0/0; 
                     G1397-DddtoxA-N (<a 
                     href=""urn:local:.:h3-dm2laik"">pKRPS5A-DIPS-1397N/C 
                     nTALECDchmYIG OleG</a>)"
                     /note="/Alternative Match=79.94%; Adjusted-Interval-0:0/0; 
                     G1397-DddtoxA-N (<a 
                     href=""urn:local:.:hf-dm2lo98"">[有村完成ver2]pKRPS5A-DIPS-1397
                     N/C ATP6-2WY OleG Copy</a>)"
                     /note="/Primary Match=79.94%; Adjusted-Interval-0:0/0; 
                     G1397-DddtoxA-N (<a 
                     href=""urn:local:.:1t-e7n3br9"">【完成】pK7WG2NLSHSP_platinum_L
                     /R:1397N/C_CYO1_ex2</a>)"
                     /note="/Transferred 
                     From=【完成】pK7WG2NLSHSP_platinum_L/R:1397N/C_CYO1_ex2"
                     /note="/Transferred Similarity=79.94%"
                     /note="/created_by=Issei.N"
                     /note="/modified by=arimura"
                     /note="/modified_by=Issei.N"
                     /note="/modified_by=ゲスト"
     misc_feature    complement(16966..16976)
                     /label=attL2
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="Geneious type: attL2"
                     /note="/Primary Match=100.00%; Adjusted-Interval-0:0/0; 
                     attL2 (<a 
                     href=""urn:local:.:d-dpikyjt"">【発現】pK7WG2_NLS_OsPE2_FYEpeg<
                     /a>)"
                     /note="/Transferred From=【発現】pK7WG2_NLS_OsPE2_FYEpeg"
                     /note="/Transferred Similarity=100.00%"
     protein_bind    complement(16977..16987)
                     /gene="mutant version of attR"
                     /label=attR2; attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /note="/Transferred From=Exported, 
                     pMDCDIPS20181026作製途中ほぼ完成？, Exported"
                     /note="/Transferred Similarity=100.00% -> 97.60%"
     misc_feature    complement(16977..16986)
                     /label=attB2
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="Geneious type: attB2"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     attB2 (<a href=""urn:local:.:25-aqqp44t"">pMDC7 
                     GFP-RFP</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     attB2 (<a href=""urn:local:.:41-aqqrq9c"">pMDCDIPS-K</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     attB2 (<a 
                     href=""urn:local:.:2a-aqqp44u"">pMDC7_TAL_ATP6-2up</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     attB2 (<a 
                     href=""urn:local:.:2a-aqqp44u"">pMDC7_TAL_ATP6-2up</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     attB2 (<a 
                     href=""urn:local:.:g3-asqd2hr"">【完成】pK7WG2DIPS_RPS5Apro - 
                     Reaction of 3 fragments Expression clone</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     attB2 (<a 
                     href=""urn:local:.:3f-aqqpt4w"">pMDC7_TAL_ATP6-2up</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     attB2 (<a 
                     href=""urn:local:.:3f-aqqpt4w"">pMDC7_TAL_ATP6-2up</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     attB2 (<a href=""urn:local:.:5j-asqci34"">pMDCDIPS-K 
                     ORF125-2</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     attB2 (<a href=""urn:local:.:5j-asqci34"">pMDCDIPS-K 
                     ORF125-2</a>)"
                     /note="/Primary Match=100.00%; Adjusted-Interval-0:0/0; 
                     attB2 (<a href=""urn:local:.:25-aqqp44t"">pMDC7 
                     GFP-RFP</a>)"
                     /note="/Transferred From=pMDC7 GFP-RFP"
                     /note="/Transferred Similarity=100.00%"
                     /note="/X-Transferred From=[mitoTAL]RPS5A-Shaorf117-1"
                     /note="/X-Transferred Similarity=100.00%"
     misc_feature    complement(16977..16986)
                     /label=attR3
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="Geneious type: attR3"
                     /note="/Alternative Match=96.00%; Adjusted-Interval-0:0/0; 
                     attR3 (<a 
                     href=""urn:local:.:3h-asqc4fa"">【E2】pENTR_R4R3_T-P-MDC_DIPS
                     </a>)"
                     /note="/Alternative Match=96.00%; Adjusted-Interval-0:0/0; 
                     attR3 (<a 
                     href=""urn:local:.:2h-a6x9gky"">【E2】pENTR_R4R3_T-HSP-P-RPS5
                     A_DIPSsig ver2</a>)"
                     /note="/Primary Match=96.00%; Adjusted-Interval-0:0/0; 
                     attR3 (<a 
                     href=""urn:local:.:m-a50mww7"">【E2】pENTR_R4R3_T-HSP-P-RPS5A
                     _DIPSsig - コピー.xdna</a>)"
                     /note="/Transferred 
                     From=【E2】pENTR_R4R3_T-HSP-P-RPS5A_DIPSsig - コピー.xdna"
                     /note="/Transferred Similarity=96.00%"
     misc_feature    complement(16977..16986)
                     /label=attR4
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="Geneious type: attR4"
                     /note="/Alternative Match=95.20%; Adjusted-Interval-0:0/0; 
                     attR4 (<a 
                     href=""urn:local:.:3h-asqc4fa"">【E2】pENTR_R4R3_T-P-MDC_DIPS
                     </a>)"
                     /note="/Alternative Match=95.20%; Adjusted-Interval-0:0/0; 
                     attR4 (<a 
                     href=""urn:local:.:2h-a6x9gky"">【E2】pENTR_R4R3_T-HSP-P-RPS5
                     A_DIPSsig ver2</a>)"
                     /note="/Primary Match=95.20%; Adjusted-Interval-0:0/0; 
                     attR4 (<a 
                     href=""urn:local:.:m-a50mww7"">【E2】pENTR_R4R3_T-HSP-P-RPS5A
                     _DIPSsig - コピー.xdna</a>)"
                     /note="/Transferred 
                     From=【E2】pENTR_R4R3_T-HSP-P-RPS5A_DIPSsig - コピー.xdna"
                     /note="/Transferred Similarity=95.20%"
     misc_feature    complement(16977..16986)
                     /label=attR1
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="Geneious type: attR1"
                     /note="/Alternative Match=97.60%; Adjusted-Interval-0:0/0; 
                     attR1 (<a href=""urn:local:.:f-b8voshw"">pDE-Cas9</a>)"
                     /note="/Alternative Match=97.60%; Adjusted-Interval-0:0/0; 
                     attR1 (<a 
                     href=""urn:local:.:14-ancw4d4"">pK7WG2DIPS_RPS5Apro 
                     ver2</a>)"
                     /note="/Alternative Match=97.60%; Adjusted-Interval-0:0/0; 
                     attR1 (<a 
                     href=""urn:local:.:2d-a6x9885"">pH7WG2DIPS_RPS5Apro 
                     ver2</a>)"
                     /note="/Alternative Match=97.60%; Adjusted-Interval-0:0/0; 
                     attR1 (<a href=""urn:local:.:3s-aqqq5bj"">【DEST】pDEST 
                     pMDCDIPS-K</a>)"
                     /note="/Primary Match=97.60%; Adjusted-Interval-0:0/0; 
                     attR1 (<a 
                     href=""urn:local:.:e-a429oh8"">【DEST】pK7WG2DIPS_RPS5Apro - 
                     コピー.xdna</a>)"
                     /note="/Transferred From=【DEST】pK7WG2DIPS_RPS5Apro - 
                     コピー.xdna"
                     /note="/Transferred Similarity=97.60%"
     misc_feature    complement(16977..16986)
                     /label=attR2
                     /note="Geneious type: attR2"
     misc_feature    complement(16977..16986)
                     /label=attR2
                     /note="Geneious type: attR2"
     misc_recomb     complement(16977..16986)
                     /label=AttR2
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     AttR2 (<a 
                     href=""urn:local:.:1w-ag1nupr"">?DEST?pK7WG2DIPS</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     AttR2 (<a 
                     href=""urn:local:.:2d-a6x9885"">pH7WG2DIPS_RPS5Apro 
                     ver2</a>)"
                     /note="/Alternative Match=100.00%; 
                     Adjusted-Interval-0:0/107; AttR2 (<a 
                     href=""urn:local:.:kt-asqdgru"">pH7WG2DIPS_RPS5Apro ver2 - 
                     Reaction of 3 fragments Expression clone</a>)"
                     /note="/Alternative Match=97.58%; Adjusted-Interval-0:0/0; 
                     AttR1 (<a 
                     href=""urn:local:.:14-ancw4d4"">pK7WG2DIPS_RPS5Apro 
                     ver2</a>)"
                     /note="/Alternative Match=97.58%; Adjusted-Interval-0:0/0; 
                     AttR1 (<a 
                     href=""urn:local:.:1w-ag1nupr"">?DEST?pK7WG2DIPS</a>)"
                     /note="/Alternative Match=97.58%; Adjusted-Interval-0:0/0; 
                     AttR1 (<a 
                     href=""urn:local:.:2d-a6x9885"">pH7WG2DIPS_RPS5Apro 
                     ver2</a>)"
                     /note="/Alternative Match=96.77%; Adjusted-Interval-0:0/0; 
                     attR3 (<a 
                     href=""urn:local:.:3h-asqc4fa"">【E2】pENTR_R4R3_T-P-MDC_DIPS
                     </a>)"
                     /note="/Alternative Match=96.77%; Adjusted-Interval-0:0/0; 
                     attR3 (<a 
                     href=""urn:local:.:2h-a6x9gky"">【E2】pENTR_R4R3_T-HSP-P-RPS5
                     A_DIPSsig ver2</a>)"
                     /note="/Alternative Match=96.77%; Adjusted-Interval-0:0/0; 
                     attR3 (<a 
                     href=""urn:local:.:j-b1wtsla"">?E2?pENTR_R4R3_T-HSP-P-35S_D
                     IPSsig????????.xdna</a>)"
                     /note="/Primary Match=100.00%; Adjusted-Interval-0:0/0; 
                     AttR2 (<a 
                     href=""urn:local:.:14-ancw4d4"">pK7WG2DIPS_RPS5Apro 
                     ver2</a>)"
                     /note="/SerialCloner_Arrow=True"
                     /note="/SerialCloner_Color=&hDDA0DD"
                     /note="/SerialCloner_Protect=False"
                     /note="/SerialCloner_Show=True"
                     /note="/Transferred From=pK7WG2DIPS_RPS5Apro ver2"
                     /note="/Transferred Similarity=100.00%"
     protein_bind    complement(16977..16986)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /note="/Transferred From=pMDCDIPS20181026作製途中ほぼ完成？"
                     /note="/Transferred Similarity=97.58%"
     misc_feature    16977..16986
                     /label=attR4
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="/Primary Match=95.97%; Adjusted-Interval-0:0/0; 
                     attR4 (<a 
                     href=""urn:local:.:j-b1wtsla"">?E2?pENTR_R4R3_T-HSP-P-35S_D
                     IPSsig????????.xdna</a>)"
                     /note="/SerialCloner_Arrow=True"
                     /note="/SerialCloner_Color=&hBD3DC0"
                     /note="/SerialCloner_Protect=True"
                     /note="/SerialCloner_Show=True"
                     /note="/Transferred 
                     From=?E2?pENTR_R4R3_T-HSP-P-35S_DIPSsig????????.xdna"
                     /note="/Transferred Similarity=95.97%"
     misc_feature    complement(16977..16986)
                     /label=attR3
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="/Primary Match=96.75%; Adjusted-Interval-0:0/0; 
                     attR3 (<a 
                     href=""urn:local:.:j-b1wtsla"">?E2?pENTR_R4R3_T-HSP-P-35S_D
                     IPSsig????????.xdna</a>)"
                     /note="/SerialCloner_Arrow=True"
                     /note="/SerialCloner_Color=&hC02E73"
                     /note="/SerialCloner_Protect=True"
                     /note="/SerialCloner_Show=True"
                     /note="/Transferred 
                     From=?E2?pENTR_R4R3_T-HSP-P-35S_DIPSsig????????.xdna"
                     /note="/Transferred Similarity=96.75%"
     misc_feature    16997..17222
                     /label=ter 35S; ter35s
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate from
                     Database' based on nucleotide similarity"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     ter 35S; ter35s (<a 
                     href=""urn:local:.:19-aqqnqw8"">【完成】pK7WG2DIPSHSP_platinumO
                     RF125_1.xdna</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     ter35s (<a 
                     href=""urn:local:.:14-ancw4d4"">pK7WG2DIPS_RPS5Apro 
                     ver2</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     ter35s (<a 
                     href=""urn:local:.:1w-ag1nupr"">?DEST?pK7WG2DIPS</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     ter35s (<a 
                     href=""urn:local:.:2d-a6x9885"">pH7WG2DIPS_RPS5Apro 
                     ver2</a>)"
                     /note="/Alternative Match=100.00%; Adjusted-Interval-0:0/0;
                     ter35s (<a 
                     href=""urn:local:.:kt-asqdgru"">pH7WG2DIPS_RPS5Apro ver2 - 
                     Reaction of 3 fragments Expression clone</a>)"
                     /note="/Primary Match=100.00%; Adjusted-Interval-0:0/0; ter
                     35S; ter35s (<a 
                     href=""urn:local:.:1a-aqqnqw8"">【完成】pK7WG2DIPSHSP_platinumO
                     RF125_2.xdna</a>)"
                     /note="/SerialCloner_Arrow=True"
                     /note="/SerialCloner_Color=&h004080; &h008040"
                     /note="/SerialCloner_Protect=False"
                     /note="/SerialCloner_Show=True"
                     /note="/Transferred 
                     From=【完成】pK7WG2DIPSHSP_platinumORF125_2.xdna"
                     /note="/Transferred Similarity=100.00%"
                     /note="/X-Transferred From=[mitoTAL]RPS5A-Shaorf117-1"
                     /note="/X-Transferred Similarity=100.00%"
ORIGIN
        1 ggccctctag aggatccccg gcaccctact tagatcaaca cataaaagtt agtaagtgaa
       61 gaaccacaac aacaacacta gattcatctt caagtgtatg taggtatagt aacatgaaca
      121 agaacagact caagtacaag atcgcatacg aaaatggaaa tggcaatgtc acttccacat
      181 aatcaaacac gaatcctcat atcaacaagg cctgagattc taactagctc ataacaactt
      241 agccaatagt tacttgagac taccaaatgt atgtagaact aaagactaag ggacagagag
      301 ttcgtctaaa caggtgaatc tagtcgttgt tatctaataa acaattcagc cccaaatgca
      361 gaacacacat agagctctct attgattcaa attacgatct gatactgata acgtctagat
      421 ttttagggtt aaagcaatca atcacctgac gattcaaggt ggttggatca tgacgattcc
      481 agaaaacatc aagcaagctc tcaaagctac actctttggg atcatactga actctaacaa
      541 cctcgttatg tcccgtagtg ccagtacaga catcctcgta actcggattg tgcacgatgc
      601 catggctata cccaacctcg gtcttggtca caccaggaac tctctggtaa gctagctcca
      661 ctccccagaa acaaccggcg ccaaattgcg cgaattgctg acctgaagac ggaacatcat
      721 cgtcgggtcc ttgggcgatt gcggcggaag atgggtcagc ttgggcttga ggacgagacc
      781 cgaatccgag tctgttgaaa aggttgttca ttggggattt gtatacggag attggtcgtc
      841 gagaggtttg agggaaagga caaatgggtt tggctctgga gaaagagagt gcggctttag
      901 agagagaatt gagaggttta gagagagatg cggcggcgat gagcggagga gagacgacga
      961 ggacctgcat tatcaaagca gtgacgtggt gaaatttgga acttttaaga ggcagataga
     1021 tttattattt gtatccattt tcttcattgt tctagaatgt cgcggaacaa attttaaaac
     1081 taaatcctaa atttttctaa ttttgttgcc aatagtggat atgtgggccg tatagaagga
     1141 atctattgaa ggcccaaacc catactgacg agcccaaagg ttcgttttgc gttttatgtt
     1201 tcggttcgat gccaacgcca cattctgagc taggcaaaaa acaaacgtgt ctttgaatag
     1261 actcctctcg ttaacacatg cagcggctgc atggtgacgc cattaacacg tggcctacaa
     1321 ttgcatgatg tctccattga cacgtgactt ctcgtctcct ttcttaatat atctaacaaa
     1381 cactcctacc tcttccaaaa tatatacaca tctttttgat caatctctca ttcaaaatct
     1441 cattctctct agtaaacaag aacaaaaaaa tggcggatac agctagagga acccatcacg
     1501 atatcatcgg cagagaccag tacccgatga tgggccgaga ccgagaccag taccagatgt
     1561 ccggacgagg atctgactac tccaagtcta ggcagattgc taaagctgca actgctgtca
     1621 cagctggtgg ttccctcctt gttctctcca gccttaccct tgttggaact gtcatagctt
     1681 tgactgttgc aacacctctg ctcgttatct tcagcccaat ccttgtcccg gctctcatca
     1741 cagttgcact cctcatcacc ggttttcttt cctctggagg gtttggcatt gccgctataa
     1801 ccgttttctc ttggatttac aagtaagcac acatttatca tcttacttca taattttgtg
     1861 caatatgtgc atgcatgtgt tgagccagta gctttggatc aatttttttg gtcgaataac
     1921 aaatgtaaca ataagaaatt gcaaattcta gggaacattt ggttaactaa atacgaaatt
     1981 tgacctagct agcttgaatg tgtctgtgta tatcatctat ataggtaaaa tgcttggtat
     2041 gatacctatt gattgtgaat aggtacgcaa cgggagagca cccacaggga tcagacaagt
     2101 tggacagtgc aaggatgaag ttgggaagca aagctcagga tctgaaagac agagctcagt
     2161 actacggaca gcaacatact ggtggggaac atgaccgtga ccgtactcgt ggtggccagc
     2221 acactactca agggtgggcg cgccgaccca gctttcttgt acaaagtggt gatatcccgc
     2281 ggatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     2341 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     2401 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     2461 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     2521 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     2581 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     2641 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     2701 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     2761 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     2821 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     2881 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     2941 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacgaac
     3001 ccggcatgtt acgtcctgta gaaaccccaa cccgtgaaat caaaaaactc gacggcctgt
     3061 gggcattcag tctggatcgc gaccgcaaac cgaagtcggc ggcttttctg ctgcaaaaac
     3121 gctggactgg catgaacttc ggtgaaaaac cgcagcaggg aggcaaacaa tgaccatggc
     3181 ggccgggagg gccatgctag agtccgcaaa aatcaccagt ctctctctac aaatctatct
     3241 ctctctattt ttctccagaa taatgtgtga gtagttccca gataagggaa ttagggttct
     3301 tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt
     3361 aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagtg acctgcaggc
     3421 atgcgacctc gaattatcga tcatgagcgg agaattaagg gagtcacgtt atgacccccg
     3481 ccgatgacgc gggacaagcc gttttacgtt tggaactgac agaaccgcaa cgttgaagga
     3541 gccactgagc cgcgggtttc tggagtttaa tgagctaagc acatacgtca gaaaccatta
     3601 ttgcgcgttc aaaagtcgcc taaggtcact atcagctagc aaatatttct tgtcaaaaat
     3661 gctccactga cgttccataa attcccctcg gtatccaatt agagtctcat attcactctc
     3721 aactcgatcg aggcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     3781 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     3841 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     3901 ccctgaatga actccaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     3961 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     4021 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     4081 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     4141 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     4201 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     4261 cgcggatgcc cgacggcgag gatctcgtcg tgacccacgg cgatgcctgc ttgccgaata
     4321 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     4381 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     4441 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     4501 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcggac tctagctaga
     4561 gtcaagcaga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     4621 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     4681 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     4741 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     4801 tgtcatctat gttactagat cgaccggcat gcaagctgat aattcaattc ggcgttaatt
     4861 cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca
     4921 ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa
     4981 tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta
     5041 aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg
     5101 ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg
     5161 ttgctgcctg tgatcaattc gggcacgaac ccagtggaca taagcctcgt tcggttcgta
     5221 agctgtaatg caagtagcgt aactgccgtc acgcaactgg tccagaacct tgaccgaacg
     5281 cagcggtggt aacggcgcag tggcggtttt catggcttct tgttatgaca tgtttttttg
     5341 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     5401 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     5461 acatcatggg ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     5521 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     5581 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     5641 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     5701 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     5761 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     5821 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     5881 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     5941 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     6001 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     6061 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     6121 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     6181 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     6241 aggtagtcgg caaataatgt ctagctagaa attcgttcaa gccgacgccg cttcgccggc
     6301 gttaactcaa gcgattagat gcactaagca cataattgct cacagccaaa ctatcaggtc
     6361 aagtctgctt ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata
     6421 tcatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6481 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6541 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6601 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     6661 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6721 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6781 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6841 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6901 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6961 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     7021 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     7081 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     7141 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     7201 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     7261 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     7321 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
     7381 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
     7441 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     7501 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg
     7561 ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga
     7621 ttgcctggcc gtaggccagc catttttgag cggccagcgg ccgcgatagg ccgacgcgaa
     7681 gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag ctcttcggct
     7741 gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt aataagtttt
     7801 aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac ttacatgtgt
     7861 gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt cccaatgtac
     7921 ggctttgggt tcccaatgta cgtgctatcc acaggaaaga gaccttttcg acctttttcc
     7981 cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc ggatgcttcg
     8041 ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc cgcctcctcc
     8101 ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt gaaacagaac
     8161 ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa ccatctggct
     8221 tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat gccgggatcg
     8281 atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg
     8341 cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt ttcgctcttt
     8401 acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag gcggccgttc
     8461 ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct
     8521 accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag tacgtccgca
     8581 acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta tcggttcatg
     8641 gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac actggccatg
     8701 ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg
     8761 ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat gctgcgacta
     8821 tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg
     8881 ggcggcttcc taatcgacgg cgcaccggct gccggcggtt gccgggattc tttgcggatt
     8941 cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc
     9001 tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc cgcgccgatt
     9061 tgtaccgggc cggatggttt gcgaccgtca cgccgattcc tcgggcttgg gggttccagt
     9121 gccattgcag ggccggcaga caacccagcc gcttacgcct ggccaaccgc ccgttcctcc
     9181 acacatgggg cattccacgg cgtcggtgcc tggttgttct tgattttcca tgccgcctcc
     9241 tttagccgct aaaattcatc tactcattta ttcatttgct catttactct ggtagctgcg
     9301 cgatgtattc agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca
     9361 gcttggtgtg atcctccgcc ggcaactgaa agttgacccg cttcatggct ggcgtgtctg
     9421 ccaggctggc caacgttgca gccttgctgc tgcgtgcgct cggacggccg gcacttagcg
     9481 tgtttgtgct tttgctcatt ttctctttac ctcattaact caaatgagtt ttgatttaat
     9541 ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg attcaagaac
     9601 ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac gggactcaag
     9661 aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt
     9721 gatcgcccgc gacacgacaa aggccgcttg tagccttcca tccgtgacct caatgcgctg
     9781 cttaaccagc tccaccaggt cggcggtggc ccatatgtcg taagggcttg gctgcaccgg
     9841 aatcagcacg aagtcggctg ccttgatcgc ggacacagcc aagtccgccg cctggggcgc
     9901 tccgtcgatc actacgaagt cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg
     9961 gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc acggccgccc aatcgcgggc
    10021 actgccctgg ggatcggaat cgactaacag aacatcggcc ccggcgagtt gcagggcgcg
    10081 ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat
    10141 aaccttcatg cgttcccctt gcgtatttgt ttatttactc atcgcatcat atacgcagcg
    10201 accgcatgac gcaagctgtt ttactcaaat acacatcacc tttttagacg gcggcgctcg
    10261 gtttcttcag cggccaagct ggccggccag gccgccagct tggcatcaga caaaccggcc
    10321 aggatttcat gcagccgcac ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc
    10381 gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg
    10441 tgcggtttca tgcttgttcc tcttggcgtt cattctcggc ggccgccagg gcgtcggcct
    10501 cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct
    10561 cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac gccaagcagt gcagccgcct
    10621 ctttcacggt gcggccttcc tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg
    10681 tgagggtagg gcgggggcca aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc
    10741 gggtgcggtc gatgattagg gaacgctcga actcggcaat gccggcgaac acggtcaaca
    10801 ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg
    10861 cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc
    10921 ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag gtggtcaagc atcctggcca
    10981 gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga aaacagcttg gtgcagccgg
    11041 ccgcgtgcag ttcggcccgt tggttggtca agtcctggtc gtcggtgctg acgcgggcat
    11101 agcccagcag gccagcggcg gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc
    11161 aagtattcta ctttatgcga ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg
    11221 cggcagcctg tcgcgtaact taggacttgt gcgacatgtc gttttcagaa gacggctgca
    11281 ctgaacgtca gaagccgact gcactatagc agcggagggg ttggatcaaa gtactttgat
    11341 cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat aaaccttttc
    11401 acgccctttt aaatatccgt tattctaata aacgctcttt tctcttaggt ttacccgcca
    11461 atatatcctg tcaaacactg atagtttaaa ctgaaggcgg gaaacgacaa tctgatccaa
    11521 gctcaagcta agctctcaac ttttgattcg ctatttgcag tgcacctgtg gcgttcatca
    11581 catcttttgt gacactgttt gcactggtca ttgctattac aaaggacctt cctgatgttg
    11641 aaggagatcg aaagtaagta actgcacgca taaccatttt ctttccgctc tttggctcaa
    11701 tccatttgac agtcaaagac aatgtttaac cagctccgtt tgatatattg tctttatgtg
    11761 tttgttcaag catgtttagt taatcatgcc tttgattgat cttgaatagg ttccaaatat
    11821 caaccctggc aacaaaactt ggagtgagaa acattgcatt cctcggttct ggacttctgc
    11881 tagtaaatta tgtttcagcc atatcactag ctttctacat gcctcaggtg aattcatcta
    11941 tttccgtctt aactatttcg gttaattaaa gcacgaacac cattactgca tgtagaagct
    12001 tgataaacta tcgccaccaa tttatttttg ttgcgatatt gttactttcc tcagtatgca
    12061 gctttgaaaa gaccaaccct cttatccttt aacaatgaac aggtttttag aggtagcttg
    12121 atgattcctg cacatgtgat cttggcttca ggcttaattt tccaggtaaa gcattatgag
    12181 atactcttat atctcttaca tacttttgag ataatgcaca agaacttcat aactatatgc
    12241 tttagtttct gcatttgaca ctgccaaatt cattaatctc taatatcttt gttgttgatc
    12301 tttggtagac atgggtacta gaaaaagcaa actacaccaa ggtaaaatac ttttgtacaa
    12361 acataaactc gttatcacgg aacatcaatg gagtgtatat ctaacggagt gtagaaacat
    12421 ttgattattg caggaagcta tctcaggata ttatcggttt atatggaatc tcttctacgc
    12481 agagtatctg ttattcccct tcctctagct ttcaatttca tggtgaggat atgcagtttt
    12541 ctttgtatat cattcttctt cttctttgta gcttggagtc aaaatcggtt ccttcatgta
    12601 catacatcaa ggatatgtcc ttctgaattt ttatatcttg caataaaaat gcttgtacca
    12661 attgaaacac cagctttttg agttctatga tcactgactt ggttctaacc aaaaaaaaaa
    12721 aaatgtttaa tttacatatc taaaagtagg tttagggaaa cctaaacagt aaaatatttg
    12781 tatattattc gaatttcact catcataaaa acttaaattg caccataaaa ttttgtttta
    12841 ctattaatga tgtaatttgt gtaacttaag ataaaaataa tattccgtaa gttaaccggc
    12901 taaaaccacg tataaaccag ggaacctgtt aaaccggttc tttactggat aaagaaatga
    12961 aagcccatgt agacagctcc attagagccc aaaccctaaa tttctcatct atataaaagg
    13021 agtgacatta gggtttttgt tcgtcctctt aaagcttctc gttttctctg ccgtctctct
    13081 cattcgcgcg acgcaaacga tcttcaggtg atcttctttc tccaaatcct ctctcataac
    13141 tctgatttcg tacttgtgta tttgagctca cgctctgttt ctctcaccac agccgtcgac
    13201 ccacgcgtcc gataatttcc ccaatttctc agagagactc aaaatcacca ccaccaccaa
    13261 atcgccgcca tgtttaaaca agcttctcgt ctcctctccc gatctgtcgc cgccgcatct
    13321 tccaaatcgg tgacgactcg tgccttttca acggaacttc catcgacgct cgattccgct
    13381 agtgatatca caagtttgta caaaaaagca ggcttttacc catacgatgt tccagattac
    13441 gctgatatcg ccgatctacg cacgctcggc tacagccagc agcaacagga gaagatcaaa
    13501 ccgaaggttc gttcgacagt ggcgcagcac cacgaggcac tggtcggcca cgggtttaca
    13561 cacgcgcaca tcgttgcgtt aagccaacac ccggcagcgt tagggaccgt cgctgtcaag
    13621 tatcaggaca tgatcgcagc gttgccagag gcgacacacg aagcgatcgt tggcgtcggc
    13681 aaacagtggt ccggcgcacg cgctctggag gccttgctca cggtggcggg agagttgaga
    13741 ggtccaccgt tacagttgga cacaggccaa cttctcaaga ttgcaaaacg tggcggcgtg
    13801 accgcagtgg aggcagtgca tgcatggcgc aatgcactga cgggtgcccc gctcaagctg
    13861 accccagacc aggttgtggc catcgccagc aacataggtg gcaagcaggc cctcgaaacc
    13921 gtccagagac tgttaccggt tctctgccag gaccacggcc tgaccccaga acaagttgtc
    13981 gcgattgcaa gcaacaacgg aggcaaacaa gccttagaaa cagtccagag attgttgccg
    14041 gtgctgtgcc aagcccacgg cctgaccccc gaccaggttg tcgctattgc tagtaacggc
    14101 ggaggcaaac aggcgctgga aacagttcag cgcctcttgc cggtcttgtg tcaggcccac
    14161 ggcctgaccc ccgcccaggt tgtcgctatt gctagtaacg gcggaggcaa acaggcgctg
    14221 gaaacagttc agcgcctctt gccggtcttg tgtcaggacc acggcctgac cccagaccag
    14281 gttgtggcca tcgccagcaa cataggtggc aagcaggccc tcgaaaccgt ccagagactg
    14341 ttaccggttc tctgccagga ccacggcctg accccagaac aagttgtcgc gattgcaagc
    14401 aacaacggag gcaaacaagc cttagaaaca gtccagagat tgttgccggt gctgtgccaa
    14461 gcccacggcc tgaccccaga ccaggttgtg gccatcgcca gcaacatagg tggcaagcag
    14521 gccctcgaaa ccgtccagag actgttaccg gttctctgcc aggcccacgg cctgacccca
    14581 gcccaggttg tggccatcgc cagcaacata ggtggcaagc aggccctcga aaccgtccag
    14641 agactgttac cggttctctg ccaggaccac ggcctgaccc cagaccaggt tgtggccatc
    14701 gccagcaaca taggtggcaa gcaggccctc gaaaccgtcc agagactgtt accggttctc
    14761 tgccaggacc acggcctgac cccagaacag gttgtggcca tcgccagcaa cataggtggc
    14821 aagcaggccc tcgaaaccgt ccagagactg ttaccggttc tctgccaggc ccacggcctg
    14881 accccagacc aggttgtggc catcgccagc aacataggtg gcaagcaggc cctcgaaacc
    14941 gtccagagac tgttaccggt tctctgccag gcccacggcc tgaccccggc ccaggtggtt
    15001 gcaatcgcgt cacacgatgg gggaaagcag gccctagaaa ccgttcagcg actcctgccc
    15061 gtcctgtgcc aggaccacgg cctgacccca gaccaggttg tggccatcgc cagcaacata
    15121 ggtggcaagc aggccctcga aaccgtccag agactgttac cggttctctg ccaggaccac
    15181 ggcctgaccc cagaacaagt tgtcgcgatt gcaagcaaca acggaggcaa acaagcctta
    15241 gaaacagtcc agagattgtt gccggtgctg tgccaagccc acggcctgac cccagaccaa
    15301 gttgtcgcga ttgcaagcaa caacggaggc aaacaagcct tagaaacagt ccagagattg
    15361 ttgcctgtgc tgtgccaagc ccacggcctg accccggccc aggtggttgc aatcgcgtca
    15421 cacgatgggg gaaagcaggc cctagaaacc gttcagcgac tcctgcccgt cctgtgccag
    15481 gaccacggcc tgaccccaga ccaagttgtc gcgattgcaa gcaacaacgg aggcaaacaa
    15541 gccttagaaa cagtccagag attgttgccg gtgctgtgcc aagaccacgg cctgaccccc
    15601 gaacaggttg tcgctattgc tagtaacggc ggaggcaaac aggcgctgga aacagttcag
    15661 cgcctcttgc cggtcttgtg tcaggcccac ggcctgaccc cggaccaggt ggttgcaatc
    15721 gcgtcacacg atgggggaaa gcaggcccta gaaaccgttc agcgactcct gcccgtcctg
    15781 tgccaggccc acggcacccc tgagcaggta gtggctattg catccaacaa cgggggcaga
    15841 cccgcactgg agtcaattgt tgcccagtta tctcgccctg atccggcgtt ggccgcgttg
    15901 accaacgacc acctcgtcgc cttggcctgc ctcggcgggc gtcctgcgct ggatgcagtg
    15961 aaaaagggat tgggggggtc tggatcgtat gctttaggac cgtatcagat ctcaagtgaa
    16021 gtcgagtttt cgcatgagta ctggatgaga catgcgttga cgttagccaa acgtgcaaga
    16081 gatgagagag aagtacctgt tggagctgtg ttggtcctca acaatcgcgt aattggagaa
    16141 ggctggaata gagccatagg tcttcacgat ccaacagccc atgcagagat catggctctc
    16201 agacaaggag gtcttgttat gcagaattac cgtctgatag atgcaacgtt gtatgtgacc
    16261 tttgagcctt gtgttatgtg tgcaggcgcg atgatccact ctcggattgg acgtgtagta
    16321 tttggagtgc gaaacagcaa aaggggtgct gctggttctc tgatgaatgt ccttaactat
    16381 ccgggtatga accatagggt ggaaatcaca gagggcattc tagctgacga atgtgctgct
    16441 ttgctttgcg acttctaccg aatgcctaga caggtgttca atgcacagaa gaaagcgcaa
    16501 agctcgatca acctcgttgg atcagggtct tatgctcttg gaccctatca gatttcagcg
    16561 cctcaattac cggcttataa tgggcaaaca gttggaacct tctactatgt caacgatgct
    16621 ggtggtcttg agagtaaggt gttttcatca ggaggtccaa ctccataccc aaactacgct
    16681 aatgcagggc atgttgcagg acaatctgct ttgttcatga gggataatgg gataagtgaa
    16741 gggctagtct tccacaacaa tccggaagga acttgcggtt tttgcgtgaa catgactgaa
    16801 actctcttac ctgagaatgc caagatgact gttgttcctc cagaaggcgc tattcccgtt
    16861 aaacggggag caactggtga gacaaaggtt tttaccggca actccaattc cccaaaatct
    16921 cctacaaagg gaggttgttg ataactcgag cgatcctcta gaacccagct ttcttgtaca
    16981 aagtggtgat atcccgcggc catgctagag tccgcaaaaa tcaccagtct ctctctacaa
    17041 atctatctct ctctattttt ctccagaata atgtgtgagt agttcccaga taagggaatt
    17101 agggttctta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt
    17161 gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa atccagtgac
    17221 ctgcaggcat gcgacgtcg
//
