LOCUS       pSL4369        5003 bp DNA     circular SYN 23-OCT-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 24, 2023 from SnapGene 7.0.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..42
                     /label=J23119 promoter
                     /note="note: bacterial promoter (Registry of Standard 
                     Biological Parts BBa_J23119)"
     misc_feature    61..283
                     /label=omega-RNA
                     /label=GstTnpB2_wRNA
                     /note="note: 
                     ApEinfo_fwdcolor=#b7e6d7ApEinfo_revcolor=#b7e6d7 label: 
                     GstTnpB3_wRNA"
     misc_feature    284..323
                     /label=tSL0496 (Guide)
                     /label=GstTnpB2_Native_TS (tSL0496)
                     /note="note: 
                     ApEinfo_fwdcolor=#b1ff67ApEinfo_revcolor=#b1ff67 label: 
                     GstTnpB3_Native_TS (tSL0496)"
     terminator      340..387
                     /label=T7 terminator
                     /note="note: 
                     ApEinfo_fwdcolor=#c6c9d1ApEinfo_revcolor=#c6c9d1 label: T7 
                     terminator"
     promoter        399..433
                     /label=J23105 Promoter
                     /note="note: 
                     ApEinfo_fwdcolor=#d6b295ApEinfo_revcolor=#d6b295 label: 
                     J23105 Promoter"
     misc_feature    452..457
                     /label=RBS
                     /note="note: 
                     ApEinfo_fwdcolor=#b4abacApEinfo_revcolor=#b4abac label: 
                     RBS"
     CDS             465..1715
                     /codon_start=1
                     /label=GstTnpB3
                     /translation="MGYFCIKQQLNGLTKEEYLTLRELCHIAKNMYNVGLYNVRQYYFE
                     HKEFLNYEKNYHLAKTNENYKLLNSNMAQQILKKVNEAFKSFFGLISLAKQGKYDHKAI
                     SIPKYLKKDGFHSLIIGQIRIDGNKFTIPYSRLFKKTHKPITITIPPVLLDKKIKQIEI
                     IPKHHARFFEIQYKYEMPEDQRELNDQKALAIDLGLNNLATCVTSDGRSFIIDGRRLKS
                     INQWFNKENARLQSIKDKQKIKGTTRKQALLAMNRNNKVNDYINKTCRYIINYCIENQI
                     GKLVIGYAETWQRNMNLGKKTNQNFVNIPLGNIKEKLEYLCEFYGIEFLKQEESYTSQA
                     SFFDGDEIPEYNADNPKEYKFSGKRIKRGLYRTKSGKLINADVNGALNILKKSKAVDLS
                     VLCSSGEVDTPQRIRIA"
     terminator      1748..1795
                     /label=T7 terminator
                     /note="note: 
                     ApEinfo_fwdcolor=#c6c9d1ApEinfo_revcolor=#c6c9d1 label: T7 
                     terminator"
     CDS             complement(1966..2757)
                     /label=SmR
                     /note="note: 
                     ApEinfo_fwdcolor=#b7e6d7ApEinfo_revcolor=#b7e6d7 label: 
                     SmR"
     promoter        complement(2758..2849)
                     /label=AmpR promoter
                     /note="note: 
                     ApEinfo_fwdcolor=#c6c9d1ApEinfo_revcolor=#c6c9d1 label: 
                     AmpR promoter"
     rep_origin      complement(2897..3635)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="note: 
                     ApEinfo_fwdcolor=#ffef86ApEinfo_revcolor=#ffef86 direction:
                     LEFT label: CloDF13 ori"
     CDS             complement(3845..4927)
                     /label=lacI
                     /note="note: 
                     ApEinfo_fwdcolor=#993366ApEinfo_revcolor=#993366 label: 
                     lacI"
     promoter        complement(join(4928..5003,1..2))
                     /label=lacI promoter
                     /note="note: 
                     ApEinfo_fwdcolor=#c6c9d1ApEinfo_revcolor=#c6c9d1 label: 
                     lacI promoter"
ORIGIN
        1 tccgggattg acagctagct cagtcctagg tataatgcta gcgtggagat atacctcgag
       61 ttaagcgcgg cttgtatcga acaaagtctg gcaaactaat taatgctgat gtcaatggcg
      121 cattaaacat cttaaagaaa agtaaagctg tagacctgag tgtcttatgc tctagcggcg
      181 aagtggacac gcctcaaaga ataaggattg cttgaagcag tcaaacttct ttggaagccc
      241 ccacttcaaa ttttcgctag aaaattaagt gggggtagtt cacggacaag tggttcacca
      301 tgcgttgctt tatggtatga tagctcgcgg ccgcaataac tagcataacc ccttggggcc
      361 tctaaacggg tcttgagggg ttttttgctg aagtcgattt tacggctagc tcagtcctag
      421 gtactatgct agcgtggaga tatacggtac caaggagata taccatgggc tatttttgca
      481 tcaagcagca gcttaatggg ttgacgaagg aagagtacct gacactgcgt gagttgtgcc
      541 atattgccaa aaacatgtac aacgttggcc tttacaacgt acgtcagtac tattttgagc
      601 ataaggagtt cttaaattac gaaaagaact atcacttggc gaagactaat gaaaactaca
      661 agttactgaa ctcgaacatg gctcaacaaa tcttgaaaaa agtaaatgaa gccttcaaaa
      721 gtttcttcgg tcttatttcg cttgccaaac aaggtaaata cgaccataaa gcaatctcga
      781 ttcccaaata cttaaagaaa gacggtttcc attcgttaat catcggccaa atccgcattg
      841 acggaaataa gttcacgatt ccatactctc gcttattcaa aaagacacat aagcccatta
      901 caatcactat tccaccagtg ttacttgata aaaagattaa gcagatcgaa atcattccga
      961 aacaccatgc acgtttcttc gagatccagt acaagtacga aatgcctgaa gatcagcgcg
     1021 aacttaatga tcagaaggcg cttgcgattg acttggggct taataacttg gctacatgtg
     1081 taacctcaga cggacgttct ttcattattg acggccgtcg tcttaaatct atcaaccaat
     1141 ggttcaataa ggaaaacgct cgcttgcagt ctattaaaga taaacagaag attaagggaa
     1201 ccacgcgtaa acaagcactg cttgcgatga atcgtaataa caaggtgaac gactatatca
     1261 ataagacctg ccgctatatc attaactact gtatcgaaaa tcaaattggc aagctggtaa
     1321 tcgggtacgc cgaaacatgg cagcgcaaca tgaatttggg caagaaaacc aaccaaaact
     1381 tcgtcaatat ccctcttgga aacattaagg agaagttaga atacctttgt gagttctatg
     1441 ggatcgagtt tttgaagcaa gaagagtcgt atacaagtca agcgtcgttt tttgatggag
     1501 atgaaatccc cgaatacaac gcggataatc ccaaagagta caaattttct ggtaagcgca
     1561 ttaagcgcgg cctgtatcgc actaaatcag gaaaacttat taacgcagac gtgaatgggg
     1621 cattgaatat tttaaaaaaa agcaaggctg ttgatttgtc tgtcttgtgc agttctggtg
     1681 aggtagacac cccgcaacgt attcgcatcg cgtgataacc taggctgctg ccaccgctga
     1741 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
     1801 acctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
     1861 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcatc
     1921 gtggccggat cttgcggccc ctcggcttga acgaattgtt agacattatt tgccgactac
     1981 cttggtgatc tcgcctttca cgtagtggac aaattcttcc aactgatctg cgcgcgaggc
     2041 caagcgatct tcttcttgtc caagataagc ctgtctagct tcaagtatga cgggctgata
     2101 ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc
     2161 ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcgccagc
     2221 ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg
     2281 ttcaggaacc ggatcaaaga gttcctccgc cgctggacct accaaggcaa cgctatgttc
     2341 tcttgctttt gtcagcaaga tagccagatc aatgtcgatc gtggctggct cgaagatacc
     2401 tgcaagaatg tcattgcgct gccattctcc aaattgcagt tcgcgcttag ctggataacg
     2461 ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga gaatctcgct
     2521 ctctccaggg gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc
     2581 atcaagcctt acggtcaccg taaccagcaa atcaatatca ctgtgtggct tcaggccgcc
     2641 atccactgcg gagccgtaca aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat
     2701 gacgccaact acctctgata gttgagtcga tacttcggcg atcaccgctt ccctcatact
     2761 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     2821 atttgaatgt atttagaaaa ataaacaaat agctagctca ctcggtcgct acgctccggg
     2881 cgtgagactg cggcgggcgc tgcggacaca tacaaagtta cccacagatt ccgtggataa
     2941 gcaggggact aacatgtgag gcaaaacagc agggccgcgc cggtggcgtt tttccatagg
     3001 ctccgccctc ctgccagagt tcacataaac agacgctttt ccggtgcatc tgtgggagcc
     3061 gtgaggctca accatgaatc tgacagtacg ggcgaaaccc gacaggactt aaagatcccc
     3121 accgtttccg gcgggtcgct ccctcttgcg ctctcctgtt ccgaccctgc cgtttaccgg
     3181 atacctgttc cgcctttctc ccttacggga agtgtggcgc tttctcatag ctcacacact
     3241 ggtatctcgg ctcggtgtag gtcgttcgct ccaagctggg ctgtaagcaa gaactccccg
     3301 ttcagcccga ctgctgcgcc ttatccggta actgttcact tgagtccaac ccggaaaagc
     3361 acggtaaaac gccactggca gcagccattg gtaactggga gttcgcagag gatttgttta
     3421 gctaaacacg cggttgctct tgaagtgtgc gccaaagtcc ggctacactg gaaggacaga
     3481 tttggttgct gtgctctgcg aaagccagtt accacggtta agcagttccc caactgactt
     3541 aaccttcgat caaaccacct ccccaggtgg ttttttcgtt tacagggcaa aagattacgc
     3601 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tactgaaccg ctctagattt
     3661 cagtgcaatt tatctcttca aatgtagcac ctgaagtcag ccccatacga tataagttgt
     3721 aattctcatg ttagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct
     3781 caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt
     3841 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     3901 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca
     3961 gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc
     4021 ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga
     4081 tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatgtcc gcaccaacgc
     4141 gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca
     4201 gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca
     4261 tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt
     4321 tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg
     4381 cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat
     4441 gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa
     4501 cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga
     4561 tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc
     4621 cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa
     4681 tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca
     4741 gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg
     4801 ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca
     4861 cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg
     4921 gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa
     4981 aggttttgcg ccattcgatg gtg
//
