LOCUS       pSL4672        4932 bp DNA     circular SYN 23-OCT-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 24, 2023 from SnapGene 7.0.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(134..211)
                     /label=lacI promoter
                     /note="label: lacI promoter"
     promoter        217..251
                     /label=J23119 promoter
                     /note="label: J23119 promoter"
     CDS             270..489
                     /label=omega-RNA
                     /label=Predicted wRNA(Model)
                     /note="codon_start: 1 transl_table: 11"
     misc_feature    490..529
                     /label=tSL0557 (Guide)
                     /label=tSL0557
     terminator      546..593
                     /label=T7 terminator
                     /note="label: T7 terminator"
     promoter        606..640
                     /label=J23105 Promoter
                     /note="label: J23105 Promoter"
     misc_feature    659..664
                     /label=RBS
                     /note="label: RBS"
     CDS             672..1853
                     /codon_start=1
                     /label=GstTnpB4
                     /label=GstTnpB3
                     /note="codon_start: 1 transl_table: 1 translation: 
                     MGYRTLKTRFRAKKEVIQKLFECNRISAEVWNECLRLAKEHHLETGKWITKTELQKATK
                     GRFSIHSQSIQAVVHKYIFARDGAKEARKKGEKIKYPYKKKKHFNTKWAKDGFVLHEDG
                     TLELSLGIWNRKRQSPLVVKIDKEKLPNGKVKEIELVYDRGLWLCLSYEDGKKPKENPC
                     KNRVAIDPGEIHTIAAICENGESLIITGRKIRSIHRLRNKKLKELQKLMSRCKKGSKQW
                     KKYNRAKQYVLSKSEAQLKDALHKTTRQFVRWCLENQVKEVVIGDIEGIQRNTKKKRNK
                     KTNQKLSNWSFGKLFDYLKYKLNAEGIQIEKKDESYTSQTCPVCGKKNKSSSRNYTCQC
                     GYKRHRDIHGAMNLFAKVYYGEIRPLEFTVKPFTYRRIA*"
                     /translation="MGYRTLKTRFRAKKEVIQKLFECNRISAEVWNECLRLAKEHHLET
                     GKWITKTELQKATKGRFSIHSQSIQAVVHKYIFARDGAKEARKKGEKIKYPYKKKKHFN
                     TKWAKDGFVLHEDGTLELSLGIWNRKRQSPLVVKIDKEKLPNGKVKEIELVYDRGLWLC
                     LSYEDGKKPKENPCKNRVAIDPGEIHTIAAICENGESLIITGRKIRSIHRLRNKKLKEL
                     QKLMSRCKKGSKQWKKYNRAKQYVLSKSEAQLKDALHKTTRQFVRWCLENQVKEVVIGD
                     IEGIQRNTKKKRNKKTNQKLSNWSFGKLFDYLKYKLNAEGIQIEKKDESYTSQTCPVCG
                     KKNKSSSRNYTCQCGYKRHRDIHGAMNLFAKVYYGEIRPLEFTVKPFTYRRIA"
     terminator      1886..1933
                     /label=T7 terminator
                     /note="label: T7 terminator"
     CDS             complement(2104..2895)
                     /label=SmR
                     /note="label: SmR"
     promoter        complement(2896..2987)
                     /label=AmpR promoter
                     /note="label: AmpR promoter"
     rep_origin      complement(3035..3773)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="direction: LEFT label: CloDF13 ori"
     CDS             complement(join(3983..4932,1..133))
                     /label=lacI
                     /note="label: lacI"
ORIGIN
        1 gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt
       61 tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg
      121 ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac
      181 cgcgaaaggt tttgcgccat tcgatggtgt ccgggattga cagctagctc agtcctaggt
      241 ataatgctag cgtggagata tacctcgagt ttacggtcaa accgtttacg tatcgacgga
      301 ttgcttaggt atgaagtcgt agatggcgag ttcccgccct tgagtatcat ggatactcat
      361 gttgcctgtg gctcccaacg ggacgggtgc caggtgttgc ctgcagatag gctgccaacc
      421 agcctatcat gcatccacct ccgaagggga aacaggaaac ccccacttcg ataagtgggg
      481 gaggttcatc ggtcattttc ccgtgttttc gttctctgtc tccagcgcac tcgcggccgc
      541 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaagt
      601 cgacttttac ggctagctca gtcctaggta ctatgctagc gtggagatat acggtaccaa
      661 ggagatatac catgggctac cgcacactga aaactcgttt ccgcgccaag aaagaagtta
      721 tccagaagtt gtttgaatgc aaccgtatct ccgcggaggt ttggaacgag tgcctgcgct
      781 tagctaaaga gcatcacctt gagaccggca agtggattac taaaacggaa cttcaaaaag
      841 ccacgaaggg ccgcttttct attcatagcc agagtattca agcggtagtt cataagtaca
      901 ttttcgcgcg cgatggggct aaagaggccc gtaagaaagg agaaaaaatc aagtatccat
      961 acaaaaaaaa gaagcacttc aatactaagt gggctaaaga tggcttcgtg ttgcatgaag
     1021 acggaacgct tgaattgtcg ttaggaatct ggaatcgtaa gcgtcaatcg cctttggtgg
     1081 tgaaaatcga taaagagaag ttaccaaacg gcaaagtaaa agaaattgag ttggtctatg
     1141 accgcgggtt gtggttatgc ttgagctatg aagacggcaa aaaacctaaa gaaaaccctt
     1201 gtaagaatcg cgtagcaatc gacccaggag agatccacac gattgcagcg atctgtgaga
     1261 atggcgaatc gctgattatt actggtcgta aaatccgctc gattcaccgc cttcgtaaca
     1321 agaaacttaa agaattgcag aagttgatgt cgcgctgtaa gaaggggagt aagcagtgga
     1381 aaaaatataa ccgtgccaag cagtatgttt tatcaaaatc ggaagcccag cttaaagatg
     1441 ctttgcacaa aacgacccgc caattcgttc gctggtgtct ggagaatcaa gtcaaggagg
     1501 tggtaattgg tgacatcgag ggtattcagc gtaatactaa gaagaagcgc aacaagaaaa
     1561 cgaatcaaaa gctgagtaat tggtcgttcg gtaagctgtt tgattatctg aagtataagt
     1621 taaatgccga ggggatccag attgaaaaaa aggacgaatc gtacacctca caaacctgcc
     1681 cggtctgcgg aaagaagaac aagtcgagtt cacgcaacta cacctgtcag tgtgggtata
     1741 aacgtcaccg tgatatccac ggggccatga atttattcgc aaaggtctat tacggggaga
     1801 ttcgccccct tgagtttacc gttaaaccgt tcacttaccg tcgtatcgcg taataagagc
     1861 tcctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
     1921 gaggggtttt ttgctgaaac ctcaggcatt tgagaagcac acggtcacac tgcttccggt
     1981 agtcaataaa ccggtaaacc agcaatagac ataagcggct atttaacgac cctgccctga
     2041 accgacgacc gggtcatcgt ggccggatct tgcggcccct cggcttgaac gaattgttag
     2101 acattatttg ccgactacct tggtgatctc gcctttcacg tagtggacaa attcttccaa
     2161 ctgatctgcg cgcgaggcca agcgatcttc ttcttgtcca agataagcct gtctagcttc
     2221 aagtatgacg ggctgatact gggccggcag gcgctccatt gcccagtcgg cagcgacatc
     2281 cttcggcgcg attttgccgg ttactgcgct gtaccaaatg cgggacaacg taagcactac
     2341 atttcgctca tcgccagccc agtcgggcgg cgagttccat agcgttaagg tttcatttag
     2401 cgcctcaaat agatcctgtt caggaaccgg atcaaagagt tcctccgccg ctggacctac
     2461 caaggcaacg ctatgttctc ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt
     2521 ggctggctcg aagatacctg caagaatgtc attgcgctgc cattctccaa attgcagttc
     2581 gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg tgacttctac
     2641 agcgcggaga atctcgctct ctccagggga agccgaagtt tccaaaaggt cgttgatcaa
     2701 agctcgccgc gttgtttcat caagccttac ggtcaccgta accagcaaat caatatcact
     2761 gtgtggcttc aggccgccat ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg
     2821 ttcgagatgg cgctcgatga cgccaactac ctctgatagt tgagtcgata cttcggcgat
     2881 caccgcttcc ctcatactct tcctttttca atattattga agcatttatc agggttattg
     2941 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag ctagctcact
     3001 cggtcgctac gctccgggcg tgagactgcg gcgggcgctg cggacacata caaagttacc
     3061 cacagattcc gtggataagc aggggactaa catgtgaggc aaaacagcag ggccgcgccg
     3121 gtggcgtttt tccataggct ccgccctcct gccagagttc acataaacag acgcttttcc
     3181 ggtgcatctg tgggagccgt gaggctcaac catgaatctg acagtacggg cgaaacccga
     3241 caggacttaa agatccccac cgtttccggc gggtcgctcc ctcttgcgct ctcctgttcc
     3301 gaccctgccg tttaccggat acctgttccg cctttctccc ttacgggaag tgtggcgctt
     3361 tctcatagct cacacactgg tatctcggct cggtgtaggt cgttcgctcc aagctgggct
     3421 gtaagcaaga actccccgtt cagcccgact gctgcgcctt atccggtaac tgttcacttg
     3481 agtccaaccc ggaaaagcac ggtaaaacgc cactggcagc agccattggt aactgggagt
     3541 tcgcagagga tttgtttagc taaacacgcg gttgctcttg aagtgtgcgc caaagtccgg
     3601 ctacactgga aggacagatt tggttgctgt gctctgcgaa agccagttac cacggttaag
     3661 cagttcccca actgacttaa ccttcgatca aaccacctcc ccaggtggtt ttttcgttta
     3721 cagggcaaaa gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     3781 ctgaaccgct ctagatttca gtgcaattta tctcttcaaa tgtagcacct gaagtcagcc
     3841 ccatacgata taagttgtaa ttctcatgtt agtcatgccc cgcgcccacc ggaaggagct
     3901 gactgggttg aaggctctca agggcatcgg tcgagatccc ggtgcctaat gagtgagcta
     3961 acttacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     4021 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg
     4081 tggtttttct tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct
     4141 gagagagttg cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga
     4201 tggtggttaa cggcgggata taacatgagc tgtcttcggt atcgtcgtat cccactaccg
     4261 agatgtccgc accaacgcgc agcccggact cggtaatggc gcgcattgcg cccagcgcca
     4321 tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg
     4381 tttgttgaaa accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt
     4441 gattgcgagt gagatattta tgccagccag ccagacgcag acgcgccgag acagaactta
     4501 atgggcccgc taacagcgcg atttgctggt gacccaatgc gaccagatgc tccacgccca
     4561 gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat gggtgtctgg tcagagacat
     4621 caagaaataa cgccggaaca ttagtgcagg cagcttccac agcaatggca tcctggtcat
     4681 ccagcggata gttaatgatc agcccactga cgcgttgcgc gagaagattg tgcaccgccg
     4741 ctttacaggc ttcgacgccg cttcgttcta ccatcgacac caccacgctg gcacccagtt
     4801 gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg
     4861 aggtggcaac gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg
     4921 gaatgtaatt ca
//
