LOCUS       pSL5200        4862 bp DNA     circular SYN 24-OCT-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 24, 2023 from SnapGene 7.0.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4862
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(151..1233)
                     /label=lacI
                     /note="codon_start: 1 label: lacI note: label: lacI note: 
                     ApEinfo_fwdcolor=#993366ApEinfo_revcolor=#993366 
                     transl_table: 1 translation: 
                     VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGK
                     QSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQR
                     VSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVAL
                     GHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEG
                     IVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRL
                     LGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVS
                     RLESGQ*"
     promoter        complement(1234..1311)
                     /label=lacI promoter
                     /note="label: lacI promoter note: label: lacI promoter 
                     note: ApEinfo_fwdcolor=#c6c9d1ApEinfo_revcolor=#c6c9d1"
     promoter        1322..1340
                     /label=T7 promoter
                     /note="label: T7 promoter note: label: T7 promoter note: 
                     ApEinfo_fwdcolor=#c6c9d1ApEinfo_revcolor=#c6c9d1"
     misc_feature    1383..1387
                     /label=TAM
     misc_feature    1388..2842
                     /label=ISGst3
     CDS             1527..2774
                     /codon_start=1
                     /label=GstTnpB3
                     /label=GstTnpB2
                     /note="codon_start: 1 locus_tag: GS458_1585 product: 
                     transposase protein_id: ATA60033.1 transl_table: 11 
                     translation: 
                     MYFCIKQQLNGLTKEEYLTLRELCHIAKNMYNVGLYNVRQYYFEHKEFLNYEKNYHLAK
                     TNENYKLLNSNMAQQILKKVNEAFKSFFGLISLAKQGKYDHKAISIPKYLKKDGFHSLI
                     IGQIRIDGNKFTIPYSRLFKKTHKPITITIPPVLLDKKIKQIEIIPKHHARFFEIQYKY
                     EMPEDQRELNDQKALAIDLGLNNLATCVTSDGRSFIIDGRRLKSINQWFNKENARLQSI
                     KDKQKIKGTTRKQALLAMNRNNKVNDYINKTCRYIINYCIENQIGKLVIGYAETWQRNM
                     NLGKKTNQNFVNIPLGNIKEKLEYLCEFYGIEFLKQEESYTSQASFFDGDEIPEYNADN
                     PKEYKFSGKRIKRGLYRTKSGKLINADVNGALNILKKSKAVDLSVLCSSGEVDTPQRIR
                     IA*"
                     /translation="MYFCIKQQLNGLTKEEYLTLRELCHIAKNMYNVGLYNVRQYYFEH
                     KEFLNYEKNYHLAKTNENYKLLNSNMAQQILKKVNEAFKSFFGLISLAKQGKYDHKAIS
                     IPKYLKKDGFHSLIIGQIRIDGNKFTIPYSRLFKKTHKPITITIPPVLLDKKIKQIEII
                     PKHHARFFEIQYKYEMPEDQRELNDQKALAIDLGLNNLATCVTSDGRSFIIDGRRLKSI
                     NQWFNKENARLQSIKDKQKIKGTTRKQALLAMNRNNKVNDYINKTCRYIINYCIENQIG
                     KLVIGYAETWQRNMNLGKKTNQNFVNIPLGNIKEKLEYLCEFYGIEFLKQEESYTSQAS
                     FFDGDEIPEYNADNPKEYKFSGKRIKRGLYRTKSGKLINADVNGALNILKKSKAVDLSV
                     LCSSGEVDTPQRIRIA"
     misc_feature    2620..2842
                     /label=omega-RNA
                     /label=wRNA
     misc_feature    2843..2882
                     /label=Guide
     terminator      2916..2963
                     /label=T7 terminator
                     /note="label: T7 terminator note: label: T7 terminator 
                     note: ApEinfo_fwdcolor=#c6c9d1ApEinfo_revcolor=#c6c9d1"
     CDS             complement(3134..3925)
                     /label=SmR
                     /note="codon_start: 1 label: SmR note: label: SmR note: 
                     ApEinfo_fwdcolor=#b7e6d7ApEinfo_revcolor=#b7e6d7 
                     transl_table: 1 translation: 
                     MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLD
                     ETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDIL
                     AGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSPP
                     DWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEE
                     DRLASRADQLEEFVHYVKGEITKVVGK*"
     promoter        complement(3926..4017)
                     /label=AmpR promoter
                     /note="label: AmpR promoter note: label: AmpR promoter 
                     note: ApEinfo_fwdcolor=#c6c9d1ApEinfo_revcolor=#c6c9d1"
     rep_origin      complement(4065..4803)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="direction: LEFT label: CloDF13 ori note: label: 
                     CloDF13 ori note: 
                     ApEinfo_fwdcolor=#ffef86ApEinfo_revcolor=#ffef86 direction:
                     LEFT"
ORIGIN
        1 agtcagcccc atacgatata agttgtaatt ctcatgttag tcatgccccg cgcccaccgg
       61 aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga
      121 gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
      181 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
      241 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc
      301 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc
      361 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc
      421 cactaccgag atgtccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc
      481 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat
      541 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg
      601 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac
      661 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc
      721 cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc
      781 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc
      841 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg
      901 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc
      961 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc
     1021 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac
     1081 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc
     1141 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata
     1201 ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc
     1261 cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggagaaa
     1321 ttaatacgac tcactatagg cctcgagatt ggcggagcgg gcctcgtaaa aggctcgttt
     1381 tttttatctt aattaggcga gaagactccc acttcaacga agcgaagcgg agtaagtggg
     1441 agatgaatcg ccttaactat attttgctga aaataggata gattcagtaa aatatagtat
     1501 catatagtta gatgggaggt gaaaacatgt atttttgtat caaacaacag ctaaatggtt
     1561 tgaccaaaga agaatacttg actcttcgag aactgtgcca tattgccaag aacatgtaca
     1621 acgtcggatt gtacaatgtc agacaatact attttgaaca caaggaattt cttaattatg
     1681 agaaaaacta tcatcttgca aaaactaacg aaaactataa gctgttaaac agcaacatgg
     1741 cacagcaaat tttaaaaaag gttaatgaag cctttaaatc tttctttggt ttgatcagtc
     1801 ttgccaaaca aggaaaatat gaccacaagg ctatcagtat tccaaaatat cttaaaaaag
     1861 atggctttca ttcactgatc attggccaga ttcgtataga cggcaacaaa ttcacgatac
     1921 cgtattctcg cctatttaaa aagactcaca agcctatcac gataacgatt ccgcctgtgt
     1981 tactggacaa aaagattaag cagattgaaa tcattcctaa gcatcatgcc aggttctttg
     2041 agattcagta caaatatgaa atgcctgaag atcaaagaga attaaacgac caaaaagcac
     2101 tggcaattga tttaggatta aacaatcttg ccacttgtgt cacatcagac ggcagatcat
     2161 tcatcattga tgggcggaga ttaaaaagta taaatcaatg gtttaacaaa gaaaatgcca
     2221 gacttcaaag cataaaagat aagcaaaaaa tcaaaggcac cactcgtaaa caggctttgc
     2281 ttgctatgaa tcgcaataat aaagtgaatg attatatcaa caagacttgc cgttacatca
     2341 ttaactactg tattgaaaat caaattggca aacttgtcat tggctatgcg gaaacatggc
     2401 aacgcaatat gaatctagga aaaaagacaa atcaaaactt tgtcaatatt cctctcggta
     2461 acataaaaga aaaactagaa tatctttgtg aattttacgg cattgaattc ttgaaacagg
     2521 aagaatccta tacgtctcaa gccagctttt ttgacggcga tgagattcct gaatataatg
     2581 ccgacaatcc aaaagaatat aagttcagcg gcaaacgtat taagcgcggc ttgtatcgaa
     2641 caaagtctgg caaactaatt aatgctgatg tcaatggcgc attaaacatc ttaaagaaaa
     2701 gtaaagctgt agacctgagt gtcttatgct ctagcggcga agtggacacg cctcaaagaa
     2761 taaggattgc ttgaagcagt caaacttctt tggaagcccc cacttcaaat tttcgctaga
     2821 aaattaagtg ggggtagttc acggacaagt ggttcaccat gcgttgcttt atggtatgat
     2881 agagcgccta ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
     2941 aacgggtctt gaggggtttt ttgctgaaac ctcaggcatt tgagaagcac acggtcacac
     3001 tgcttccggt agtcaataaa ccggtaaacc agcaatagac ataagcggct atttaacgac
     3061 cctgccctga accgacgacc gggtcatcgt ggccggatct tgcggcccct cggcttgaac
     3121 gaattgttag acattatttg ccgactacct tggtgatctc gcctttcacg tagtggacaa
     3181 attcttccaa ctgatctgcg cgcgaggcca agcgatcttc ttcttgtcca agataagcct
     3241 gtctagcttc aagtatgacg ggctgatact gggccggcag gcgctccatt gcccagtcgg
     3301 cagcgacatc cttcggcgcg attttgccgg ttactgcgct gtaccaaatg cgggacaacg
     3361 taagcactac atttcgctca tcgccagccc agtcgggcgg cgagttccat agcgttaagg
     3421 tttcatttag cgcctcaaat agatcctgtt caggaaccgg atcaaagagt tcctccgccg
     3481 ctggacctac caaggcaacg ctatgttctc ttgcttttgt cagcaagata gccagatcaa
     3541 tgtcgatcgt ggctggctcg aagatacctg caagaatgtc attgcgctgc cattctccaa
     3601 attgcagttc gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg
     3661 tgacttctac agcgcggaga atctcgctct ctccagggga agccgaagtt tccaaaaggt
     3721 cgttgatcaa agctcgccgc gttgtttcat caagccttac ggtcaccgta accagcaaat
     3781 caatatcact gtgtggcttc aggccgccat ccactgcgga gccgtacaaa tgtacggcca
     3841 gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac ctctgatagt tgagtcgata
     3901 cttcggcgat caccgcttcc ctcatactct tcctttttca atattattga agcatttatc
     3961 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     4021 ctagctcact cggtcgctac gctccgggcg tgagactgcg gcgggcgctg cggacacata
     4081 caaagttacc cacagattcc gtggataagc aggggactaa catgtgaggc aaaacagcag
     4141 ggccgcgccg gtggcgtttt tccataggct ccgccctcct gccagagttc acataaacag
     4201 acgcttttcc ggtgcatctg tgggagccgt gaggctcaac catgaatctg acagtacggg
     4261 cgaaacccga caggacttaa agatccccac cgtttccggc gggtcgctcc ctcttgcgct
     4321 ctcctgttcc gaccctgccg tttaccggat acctgttccg cctttctccc ttacgggaag
     4381 tgtggcgctt tctcatagct cacacactgg tatctcggct cggtgtaggt cgttcgctcc
     4441 aagctgggct gtaagcaaga actccccgtt cagcccgact gctgcgcctt atccggtaac
     4501 tgttcacttg agtccaaccc ggaaaagcac ggtaaaacgc cactggcagc agccattggt
     4561 aactgggagt tcgcagagga tttgtttagc taaacacgcg gttgctcttg aagtgtgcgc
     4621 caaagtccgg ctacactgga aggacagatt tggttgctgt gctctgcgaa agccagttac
     4681 cacggttaag cagttcccca actgacttaa ccttcgatca aaccacctcc ccaggtggtt
     4741 ttttcgttta cagggcaaaa gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     4801 atcttttcta ctgaaccgct ctagatttca gtgcaattta tctcttcaaa tgtagcacct
     4861 ga
//
