LOCUS       pTAB-089_AAVS_Pr       12262 bp DNA     circular SYN 25-OCT-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12262)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 25, 2023 from SnapGene Viewer 7.0.1
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..12262
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(453..505)
                     /label=AmpR promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=82.8"
                     /note="/other=promoter"
     rep_origin      576..1164
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             1347..1439
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     protein_bind    1452..1473
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        1488..1518
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    1522..1544
                     /label=lac operator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=92.0"
                     /note="/other=protein_bind"
     promoter        1587..1605
                     /label=T3 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    1640..2443
                     /label=HA-L
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     misc_feature    2450..2475
                     /label=SA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             2562..2981
                     /codon_start=1
                     /label=bsr
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MKTFNISQQDLELVEVATEKITMLYEDNKHHVGAAIRTKTGEIIS
                     AVHIEAYIGRVTVCAEAIAIGSAVSNGQKDFDTIVAVRHPYSDEVDRSIRVVSPCGMCR
                     ELISDYAPDCFVLIEMNGKLVKTTIEELIPLKYTRN"
     polyA_signal    3031..3255
                     /label=bGH poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     promoter        3259..3280
                     /label=SV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.1"
                     /note="/other=promoter"
     protein_bind    3323..3355
                     /label=attB
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=97.1"
                     /note="/other=protein_bind"
     promoter        3380..3591
                     /label=EF-1-alpha core promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     LTR             3604..3872
                     /label=5' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     CDS             3906..7166
                     /codon_start=1
                     /label=Prom1s1 WT StayGold
                     /translation="MALVLGSLLLLGLCGNSFSGGQPSSTDAPKAWNYELPATNYETQD
                     SHKAGPIGILFELVHIFLYVVQPRDFPEDTLRKFLQKAYESKIDYDKIVYYEAGIILCC
                     VLGLLFIILMPLVGYFFCMCRCCNKCGGEMHQRQKENGPFLRKCFAISLLVICIIISIG
                     IFYGFVANHQVRTRIKRSRKLADSNFKDLRTLLNETPEQIKYILAQYNTTKDKAFTDLN
                     SINSVLGGGILDRLRPNIIPVLDEIKSMATAIKETKEALENMNSTLKSLHQQSTQLSSS
                     LTSVKTSLRSSLNDPLCLVHPSSETCNSIRLSLSQLNSNPELRQLPPVDAELDNVNNVL
                     RTDLDGLVQQGYQSLNDIPDRVQRQTTTVVAGIKRVLNSIGSDIDNVTQRLPIQDILSA
                     FSVYVNNTESYIHRNLPTLEEYDSYWWLGGLVICSLLTLIVIFYYLGLLCGVCGYDRHA
                     TPTTRGCVSNTGGVFLMVGVGLSFLFCWILMIIVVLTFVFGANVEKLICEPYTSKELFR
                     VLDTPYLLNEDWEYYLSGKLFNKSKMKLTFEQVYSDCKKNRGTYGTLHLQNSFNISEHL
                     NINEHTGSISSELESLKVNLNIFLLGAAGRKNLQDFAACGIDRMNYDSYLAQTGKSPAG
                     VNLLSFAYDLEAKANSLPPGNLRNSLKRDAQTIKTIHQQRVLPIEQSLSTLYQSVKILQ
                     RTGNGLLERVTRILASLDFAQNFITNNTSSVIIEETKKYGRTIIGYFEHYLQWIEFSIS
                     EKVASCKPVATALDTAVDVFLCSYIIDPLNLFWFGIGKATVFLLPALIFAVKLAKYYRR
                     MDSEDVYDDVETIPMKNMENGNNGYHKDHVYGIHNPVMTSPSQHLEENLYFQGGGGGMA
                     STPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKGKYVCTSGKLPMSWAALGTSFGYGMK
                     YYTKYPSGLKNWFHEVMPEGFTYDRHIQYKGDGSIHAKHQHFMKNGTYHNIVEFTGQDF
                     KENSPVLTGDMNVSLPNEVQHIPRDDGVECPVTLLYPLLSDKSKCVEAHQNTICKPLHN
                     QPAPDVPYHWIRKQYTQSKDDTEERDHICQSETLEAHL"
     misc_feature    7206..7224
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=17.6"
                     /note="/other=misc_feature"
     misc_feature    7224..7812
                     /label=WPRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     polyA_signal    7844..8320
                     /label=hGH poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             8344..8424
                     /codon_start=1
                     /label=HBB (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=96.3"
                     /note="/match_length=75.0"
                     /note="/other=CDS"
                     /translation="REFTPQVQAAYQKVVAGVANALAHKYH"
     polyA_signal    8495..8550
                     /label=beta-globin poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     protein_bind    complement(8933..8955)
                     /label=lac operator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=92.0"
                     /note="/other=protein_bind"
     promoter        complement(8959..8989)
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    complement(9004..9025)
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             complement(9038..9077)
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.7"
                     /note="/other=CDS"
                     /translation="AGTTGFPTGKRAV"
     misc_feature    9099..9935
                     /label=HA-R
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     promoter        complement(9976..9994)
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             9997..10144
                     /codon_start=1
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=85.1"
                     /note="/other=CDS"
                     /translation="IHWPSFYNVVTGKTLALPNLIALQHIPLSPAGVIAKRPAPIALPN
                     SCAA"
     CDS             10154..10457
                     /codon_start=1
                     /label=ccdB (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=99.3"
                     /note="/other=CDS"
                     /translation="AV*GLHL*KREPLSSVCGCTE*YY*HAGATDGDPPGQCTSAVR*S
                     LP*TLPGGAYRG*KLAHDDHRYGQCAGLRYRGRSG*SQPPRK*HQKRH*PDVLGNI"
     CDS             10505..10774
                     /codon_start=1
                     /label=tnpA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=97.8"
                     /note="/match_length=18.9"
                     /note="/other=CDS"
                     /translation="HVESQSAETVLTPDECQLLGYLDKGKRKRKEKAGSLQWAYMAIAR
                     LGGFMDSKRTGIASWGALW*GWEALQSKLDGFLAAKDLMAQGIKL"
     CDS             10806..11604
                     /codon_start=1
                     /label=NeoR/KanR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.5"
                     /note="/match_length=100.5"
                     /note="/other=CDS"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHAWRCLLAEYHGGKWPLFWIHRLWPAGCGGPLSGH
                     SVGYP*YC*RAWRRMG*PLPRALRYRRSRFAAHRLLSPS*RVLL"
     misc_feature    complement(11625..11675)
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.2"
                     /note="/other=misc_feature"
     promoter        11702..11761
                     /label=AmpR promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=58.3"
                     /note="/other=promoter"
     CDS             complement(join(11854..12262,1..452))
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
       61 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
      121 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
      181 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
      241 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
      301 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
      361 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
      421 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
      481 atttatcagg gttattgtct catgaccaaa atcccttaac gtgagttttc gttccactga
      541 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
      601 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      661 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
      721 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
      781 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
      841 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
      901 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
      961 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1021 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     1081 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     1141 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     1201 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     1261 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     1321 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     1381 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     1441 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     1501 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     1561 tatgaccatg attacgccaa gctcagaatt aaccctcact aaagggtcta gtcctgcagg
     1621 tttaaacgaa ttcgcccttt gctttctctg accagcattc tctcccctgg gcctgtgccg
     1681 ctttctgtct gcagcttgtg gcctgggtca cctctacggc tggcccagat ccttccctgc
     1741 cgcctccttc aggttccgtc ttcctccact ccctcttccc cttgctctct gctgtgttgc
     1801 tgcccaagga tgctctttcc ggagcacttc cttctcggcg ctgcaccacg tgatgtcctc
     1861 tgagcggatc ctccccgtgt ctgggtcctc tccgggcatc tctcctccct cacccaaccc
     1921 catgccgtct tcactcgctg ggttcccttt tccttctcct tctggggcct gtgccatctc
     1981 tcgtttctta ggatggcctt ctccgacgga tgtctccctt gcgtcccgcc tccccttctt
     2041 gtaggcctgc atcatcaccg tttttctgga caaccccaaa gtaccccgtc tccctggctt
     2101 tagccacctc tccatcctct tgctttcttt gcctggacac cccgttctcc tgtggattcg
     2161 ggtcacctct cactcctttc atttgggcag ctcccctacc ccccttacct ctctagtctg
     2221 tgctagctct tccagccccc tgtcatggca tcttccaggg gtccgagagc tcagctagtc
     2281 ttcttcctcc aacccgggcc cctatgtcca cttcaggaca gcatgtttgc tgcctccagg
     2341 gatcctgtgt ccccgagctg ggaccacctt atattcccag ggccggttaa tgtggctctg
     2401 gttctgggta cttttatctg tcccctccac cccacagtgg ggcaagcttc tgacctcttc
     2461 tcttcctccc acagggcctc gagagatctg gcagcggaga gggcagagga agtcttctaa
     2521 catgcggtga cgtggaggag aatcccggcc ctaggaccgg tatgaaaaca tttaacattt
     2581 ctcaacaaga tctagaatta gtagaagtag cgacagagaa gattacaatg ctttatgagg
     2641 ataataaaca tcatgtggga gcggcaattc gtacgaaaac aggagaaatc atttcggcag
     2701 tacatattga agcgtatata ggacgagtaa ctgtttgtgc agaagccatt gcgattggta
     2761 gtgcagtttc gaatggacaa aaggattttg acacgattgt agctgttaga cacccttatt
     2821 ctgacgaagt agatagaagt attcgagtgg taagtccttg tggtatgtgt agggagttga
     2881 tttcagacta tgcaccagat tgttttgtgt taatagaaat gaatggcaag ttagtcaaaa
     2941 ctacgattga agaactcatt ccactcaaat atacccgaaa ttaatgagaa ttcggggatc
     3001 aattctctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     3061 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     3121 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     3181 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     3241 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctcgact agagcttgcg
     3301 gaacccttaa tggtaccgtc tctgccaggg cgtgcccttg ggctccccgg gcgcgactag
     3361 tgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     3421 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     3481 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     3541 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt
     3601 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc
     3661 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc
     3721 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta
     3781 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt
     3841 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgccgccg
     3901 ccaccatggc cctcgtactc ggctccctgt tgctgctggg gctgtgcggg aactcctttt
     3961 caggagggca gccttcatcc acagatgctc ctaaggcttg gaattatgaa ttgcctgcaa
     4021 caaattatga gacccaagac tcccataaag ctggacccat tggcattctc tttgaactag
     4081 tgcatatctt tctctatgtg gtacagccgc gtgatttccc agaagatact ttgagaaaat
     4141 tcttacagaa ggcatatgaa tccaaaattg attatgacaa gattgtctac tatgaagcag
     4201 ggattattct atgctgtgtc ctggggctgc tgtttattat tctgatgcct ctggtggggt
     4261 atttcttttg tatgtgtcgt tgctgtaaca aatgtggtgg agaaatgcac cagcgacaga
     4321 aggaaaatgg gcccttcctg aggaaatgct ttgcaatctc cctgttggtg atttgtataa
     4381 taataagcat tggcatcttc tatggttttg tggcaaatca ccaggtaaga acccggatca
     4441 aaaggagtcg gaaactggca gatagcaatt tcaaggactt gcgaactctc ttgaatgaaa
     4501 ctccagagca aatcaaatat atattggccc agtacaacac taccaaggac aaggcgttca
     4561 cagatctgaa cagtatcaat tcagtgctag gaggcggaat tcttgaccga ctgagaccca
     4621 acatcatccc tgttcttgat gagattaagt ccatggcaac agcgatcaag gagaccaaag
     4681 aggcgttgga gaacatgaac agcaccttga agagcttgca ccaacaaagt acacagctta
     4741 gcagcagtct gaccagcgtg aaaactagcc tgcggtcatc tctcaatgac cctctgtgct
     4801 tggtgcatcc atcaagtgaa acctgcaaca gcatcagatt gtctctaagc cagctgaata
     4861 gcaaccctga actgaggcag cttccacccg tggatgcaga acttgacaac gttaataacg
     4921 ttcttaggac agatttggat ggcctggtcc aacagggcta tcaatccctt aatgatatac
     4981 ctgacagagt acaacgccaa accacgactg tcgtagcagg tatcaaaagg gtcttgaatt
     5041 ccattggttc agatatcgac aatgtaactc agcgtcttcc tattcaggat atactctcag
     5101 cattctctgt ttatgttaat aacactgaaa gttacatcca cagaaattta cctacattgg
     5161 aagagtatga ttcatactgg tggctgggtg gcctggtcat ctgctctctg ctgaccctca
     5221 tcgtgatttt ttactacctg ggcttactgt gtggcgtgtg cggctatgac aggcatgcca
     5281 ccccgaccac ccgaggctgt gtctccaaca ccggaggcgt cttcctcatg gttggagttg
     5341 gattaagttt cctcttttgc tggatattga tgatcattgt ggttcttacc tttgtctttg
     5401 gtgcaaatgt ggaaaaactg atctgtgaac cttacacgag caaggaatta ttccgggttt
     5461 tggatacacc ctacttacta aatgaagact gggaatacta tctctctggg aagctattta
     5521 ataaatcaaa aatgaagctc acttttgaac aagtttacag tgactgcaaa aaaaatagag
     5581 gcacttacgg cactcttcac ctgcagaaca gcttcaatat cagtgaacat ctcaacatta
     5641 atgagcatac tggaagcata agcagtgaat tggaaagtct gaaggtaaat cttaatatct
     5701 ttctgttggg tgcagcagga agaaaaaacc ttcaggattt tgctgcttgt ggaatagaca
     5761 gaatgaatta tgacagctac ttggctcaga ctggtaaatc ccccgcagga gtgaatcttt
     5821 tatcatttgc atatgatcta gaagcaaaag caaacagttt gcccccagga aatttgagga
     5881 actccctgaa aagagatgca caaactatta aaacaattca ccagcaacga gtccttccta
     5941 tagaacaatc actgagcact ctataccaaa gcgtcaagat acttcaacgc acagggaatg
     6001 gattgttgga gagagtaact aggattctag cttctctgga ttttgctcag aacttcatca
     6061 caaacaatac ttcctctgtt attattgagg aaactaagaa gtatgggaga acaataatag
     6121 gatattttga acattatctg cagtggatcg agttctctat cagtgagaaa gtggcatcgt
     6181 gcaaacctgt ggccaccgct ctagatactg ctgttgatgt ctttctgtgt agctacatta
     6241 tcgacccctt gaatttgttt tggtttggca taggaaaagc tactgtattt ttacttccgg
     6301 ctctaatttt tgcggtaaaa ctggctaagt actatcgtcg aatggattcg gaggacgtgt
     6361 acgatgatgt tgaaactata cccatgaaaa atatggaaaa tggtaataat ggttatcata
     6421 aagatcatgt atatggtatt cacaatcctg ttatgacaag cccatcacaa catctcgagg
     6481 agaatttgta ctttcaaggc ggtggaggag ggatggcctc aacacccttc aagttccaat
     6541 tgaagggcac aatcaacggt aaatctttca ctgtggaggg cgagggggag ggcaacagcc
     6601 atgaagggtc tcacaaaggc aaatacgtgt gcacgagcgg gaagctgccc atgtcatggg
     6661 ccgctctggg gacaagcttt gggtatggaa tgaaatacta cactaagtat cctagtgggc
     6721 ttaagaactg gtttcacgag gtgatgcctg aaggcttcac atacgatagg catattcagt
     6781 acaaaggcga cggaagtatc catgcaaagc accaacattt tatgaagaat gggacctatc
     6841 ataacatcgt ggaatttact ggccaggact ttaaggaaaa cagccccgta ctgacaggag
     6901 acatgaatgt cagtctccct aacgaggtgc agcatatccc ccgagacgac ggcgtggagt
     6961 gtcccgtgac tctcctgtac ccgctcctct ccgataagtc caagtgtgtg gaggcccacc
     7021 aaaatactat ctgtaaacct ctgcataatc agcccgctcc cgatgtacca taccattgga
     7081 ttcggaagca gtacacccag agcaaggatg acaccgaaga gcgggatcat atatgtcaaa
     7141 gcgaaaccct cgaggcccac ctctaacctg caggcggatc cgcgtttaaa ctcgaggtta
     7201 attaagatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     7261 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     7321 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     7381 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     7441 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     7501 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     7561 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     7621 catcgtcctt tccttggctg ctcgcctatg ttgccacctg gattctgcgc gggacgtcct
     7681 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     7741 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     7801 ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc tacgggtggc atccctgtga
     7861 cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt
     7921 cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg
     7981 gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg
     8041 ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc
     8101 ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg
     8161 catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc
     8221 caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct
     8281 gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgattttgt aggtaaccag
     8341 gcgcgtgaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc
     8401 aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac
     8461 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca
     8521 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat
     8581 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct
     8641 gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc
     8701 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt
     8761 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt
     8821 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc
     8881 gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     8941 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     9001 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     9061 aaacctgtcg tgccagcgga tcgacagtac taagctttac tagggacagg attggtgaca
     9121 gaaaagcccc atccttaggc ctcctccttc ctagtctcct gatattgggt ctaaccccca
     9181 cctcctgtta ggcagattcc ttatctggtg acacaccccc atttcctgga gccatctctc
     9241 tccttgccag aacctctaag gtttgcttac gatggagcca gagaggatcc tgggagggag
     9301 agcttggcag ggggtgggag ggaagggggg gatgcgtgac ctgcccggtt ctcagtggcc
     9361 accctgcgct accctctccc agaacctgag ctgctctgac gcggctgtct ggtgcgtttc
     9421 actgatcctg gtgctgcagc ttccttacac ttcccaagag gagaagcagt ttggaaaaac
     9481 aaaatcagaa taagttggtc ctgagttcta actttggctc ttcacctttc tagtccccaa
     9541 tttatattgt tcctccgtgc gtcagtttta cctgtgagat aaggccagta gccagccccg
     9601 tcctggcagg gctgtggtga ggaggggggt gtccgtgtgg aaaactccct ttgtgagaat
     9661 ggtgcgtcct aggtgttcac caggtcgtgg ccgcctctac tccctttctc tttctccatc
     9721 cttctttcct taaagagtcc ccagtgctat ctgggacata ttcctccgcc cagagcaggg
     9781 tcccgcttcc ctaaggccct gctctgggct tctgggtttg agtccttggc aagcccagga
     9841 gaggcgctca ggcttccctg tcccccttcc tcgtccacca tctcatgccc ctggctctcc
     9901 tgccccttcc ctacaggggt tcctggctct gctctaaggg caagggcgaa ttcgcggccg
     9961 ctaaattcaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac
    10021 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
    10081 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
    10141 gcctatacgt acggcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt
    10201 ttgtggatgt acagagtgat attattgaca cgccggggcg acggatggtg atccccctgg
    10261 ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg
    10321 gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg
    10381 gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga
    10441 tgttctgggg aatataaatg tcaggcatga gattatcaaa aaggatcttc acctagatcc
    10501 ttttcacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact
    10561 gggctatctg gacaagggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc
    10621 ttacatggcg atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag
    10681 ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc
    10741 cgccaaggat ctgatggcgc aggggatcaa gctctgatca agagacagga tgaggatcgt
    10801 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
    10861 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
    10921 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
    10981 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
    11041 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
    11101 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
    11161 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
    11221 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
    11281 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc
    11341 ccgacggcga ggatctcgtc gtgacccatg catggcgatg cctgcttgcc gaatatcatg
    11401 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
    11461 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
    11521 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
    11581 cgccttcttg acgagttctt ctgaattatt aacgcttaca atttcctgat gcggtatttt
    11641 ctccttacgc atctgtgcgg tatttcacac cgcatcaggt ggcacttttc ggggaaatgt
    11701 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
    11761 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    11821 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    11881 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    11941 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    12001 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    12061 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    12121 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    12181 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    12241 agttacatga tcccccatgt tg
//
