LOCUS       Exported                8627 bp DNA     circular SYN 20-OCT-2022
DEFINITION  Expression  of base editor in mammalian cells and for cell sorting .
ACCESSION   .
VERSION     .
KEYWORDS    3075
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8627)
  TITLE     HOLD - Continuous evolution of mitochondria base editors with 
            improved activity and expanded targeting scope
REFERENCE   2  (bases 1 to 8627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 24, 2023 from SnapGene 6.0.0
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1980..2032
                     /codon_start=1
                     /label=C-term
                     /translation="ACLGGRPALDAVKKGLG"
     CDS             2141..2389
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             2399..2455
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2456..3172
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2501..2522)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2762..2781)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3109..3130
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     rep_origin      3372..3800
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3459..3478)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3669..3690
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3809..3829)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3814..4143
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3994..4129
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4056..4075
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     misc_feature    4169..4210
                     /label=puroRF
     CDS             4192..4788
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    complement(4771..4808)
                     /label=puroRR
     polyA_signal    4915..5036
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4952..4971)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5006..5025
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5085..5101)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5085..5101)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5098..5120)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5109..5125
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5133..5163)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5178..5199
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5316..5333)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5487..6075)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5567..5586)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6246..7106)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6869..6888
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7107..7211)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7286..7305)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7477..7856
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7857..8060
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8011..8031
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8105..8124
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8105..8123
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             8239..8265
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8269..8295
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8299..8325
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(8329..8627,1..2032)
                     /codon_start=1
                     /label=mitoTALE
                     /translation="DIADLRTLGYSQQQQEKIKPKVRSTVAQHHEALVGHGFTHAHIVA
                     LSQHPAALGTVAVKYQDMIAALPEATHEAIVGVGKQWSGARALEALLTVAGELRGPPLQ
                     LDTGQLLKIAKRGGVTAVEAVHAWRNALTGAPLNLTPQQVVAIASNIGGKQALETVQRL
                     LPVLCQAHGLTPEQVVAIASNNGGKQALETVQALLPVLCQAHGLTPQQVVAIASNIGGK
                     QALETVQRLLPVLCQAHGLTPEQVVAIASNGGGKQALETVQRLLPVLCQAHGLTPQQVV
                     AIASNIGGKQALETVQRLLPVLCQAHGLTPEQVVAIASNNGGKQALETVQALLPVLCQA
                     HGLTPQQVVAIASNIGGKQALETVQRLLPVLCQAHGLTPQQVVAIASNIGGKQALETVQ
                     RLLPVLCQAHGLTPQQVVAIASNIGGKQALETVQRLLPVLCQAHGLTPEQVVAIASHDG
                     GKQALETVQALLPVLCQAHGLTPEQVVAIASHDGGKQALETVQALLPVLCQAHGLTPEQ
                     VVAIASNNGGKQALETVQALLPVLCQAHGLTPQQVVAIASNIGGKQALETVQRLLPVLC
                     QAHGLTPEQVVAIASHDGGKQALETVQALLPVLCQAHGLTPEQVVAIASHDGGKQALET
                     VQALLPVLCQAHGLTPEQVVAIASNGGGKQALETVQRLLPVLCQAHGLTPEQVVAIASN
                     NGGKQALETVQALLPVLCQAHGLTPQQVVAIASNNGGRPALESIVAQLSRPDPALAALT
                     NDHLVALACLGGRPALDAVKKGLG"
     CDS             8329..8627
                     /codon_start=1
                     /label=N-term
                     /translation="DIADLRTLGYSQQQQEKIKPKVRSTVAQHHEALVGHGFTHAHIVA
                     LSQHPAALGTVAVKYQDMIAALPEATHEAIVGVGKQWSGARALEALLTVAGELR"
ORIGIN
        1 tccgccgctg cagctggata caggccagct gttaaaaata gccaaaagag ggggtgtgac
       61 cgctgtggaa gccgtccatg cctggaggaa tgcactgaca ggtgcccccc ttaatctgac
      121 accacagcaa gtcgtggcaa tcgcttccaa tattgggggc aagcaggcct tggagacagt
      181 gcagcggctc cttccggttt tgtgccaggc gcacggattg accccagaac aggtggtggc
      241 aattgcatcc aacaacggag ggaagcaggc ccttgagacc gtgcaggctc tgctccctgt
      301 gctctgtcag gctcacgggc tgacacccca acaagttgtt gctattgctt caaacatcgg
      361 cggaaaacag gctctagaga ccgtccagcg tcttcttcca gtcctttgtc aagcccatgg
      421 cttaactcct gagcaggtgg tcgcaattgc cagtaacggg ggcggaaagc aagctctgga
      481 gaccgttcag cgcctactgc ccgtactgtg ccaggcccac ggattaacac ctcagcaagt
      541 ggtcgctatc gcatctaaca ttggcggtaa gcaggctctc gagactgtac agcgactgct
      601 tcccgtactc tgtcaagctc acggcctcac gccagagcaa gttgtcgcga tcgcctcaaa
      661 taatggtggc aaacaagcgc tcgaaactgt ccaagctctg ctaccagtgt tatgccaggc
      721 acatggcctc acaccgcaac aagtggtagc tattgctagt aatatcggcg gcaaacaggc
      781 acttgaaaca gtgcagaggc ttttgccagt cctgtgtcag gcccatgggt taaccccgca
      841 acaggtcgtt gctatcgcct ccaatatagg cggtaaacag gcgttggaga ctgtgcaaag
      901 gctcctgccc gtcctctgcc aagcccacgg acttacaccg cagcaggtcg tcgctatagc
      961 cagtaacatc ggaggaaaac aagccctgga aactgtacag agactgctcc cagtcttgtg
     1021 tcaagcacat gggctgaccc ccgagcaggt cgtagccatt gccagtcacg atggggggaa
     1081 acaagcctta gagacggtcc aggcactcct cccagttcta tgccaggctc atggtcttac
     1141 gccggagcag gtagtcgcta ttgcgtcaca tgacggaggg aaacaggcct tagaaacggt
     1201 tcaagcactg ctgccggttc tgtgccaagc gcacggcctg accccagagc aggttgttgc
     1261 cattgcctcc aataacggcg ggaagcaagc actggaaacc gtacaggccc tgctgcctgt
     1321 gttgtgtcag gcacacggac tgacgcccca gcaggtagtg gccattgcaa gcaacattgg
     1381 gggaaagcag gctttagaaa cagtccagcg gctgttaccc gttttatgtc aggcgcacgg
     1441 actaactcct gaacaggttg tagcaatcgc cagccacgat ggaggtaaac aagctttaga
     1501 gaccgtgcaa gctctcttac cagtactctg tcaggcccac ggcttgaccc cggaacaagt
     1561 ggtggccatc gcttcccacg atggcggtaa gcaggccttg gagaccgttc aggccttact
     1621 accggtgctc tgccaggcgc atggtttaac acccgaacaa gtcgttgcaa tcgcaagcaa
     1681 tgggggagga aagcaagccc tcgagacagt ccagaggttg cttccggtac tgtgtcaggc
     1741 tcacggccta acccctgaac aagtagtggc aatagcttct aacaatggag gcaagcaggc
     1801 attggaaaca gtgcaagccc ttctacctgt tctttgccaa gctcatggtt tgacaccaca
     1861 acaggttgtg gccatagcct ctaacaacgg tggcagaccc gccttagagt ctattgtagc
     1921 tcagctcagt agacccgacc ctgcactggc tgccctcacc aatgatcacc tggtggcgct
     1981 ggcttgcctg ggcggtcgtc cggccctgga tgcggtgaag aaaggcctgg gtggatccgc
     2041 cattccagtg aagcgcggcg ctaccggtga aaccaaagtg tttatcggta acagcaacag
     2101 cccgaagagc ccgaccaaag gcggttgctc tggcggctca actaatctga gcgacatcat
     2161 tgagaaggag actgggaaac agctggtcat tcaggagtcc atcctgatgc tgcctgagga
     2221 ggtggaggaa gtgatcggca acaagccaga gtctgacatc ctggtgcaca ccgcctacga
     2281 cgagtccaca gatgagaatg tgatgctgct gacctctgac gcccccgagt ataagccttg
     2341 ggccctggtc atccaggatt ctaacggcga gaataagatc aagatgctgg gaagcggagc
     2401 tactaacttc agcctgctga agcaggctgg agacgtggag gagaaccctg gacctatggt
     2461 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     2521 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     2581 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     2641 gaccaccctg acctatggag tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     2701 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     2761 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     2821 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     2881 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     2941 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     3001 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     3061 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     3121 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtgataagg
     3181 ggtctttgtc ctctgtactg tctctctcct tgcccctaac ccaaaaagct tcatttttct
     3241 gtgtaggctg cacaagagcc ttgattgaag atatattctt tctgaacagt atttaaggtt
     3301 tccaataaaa tgtacacccc tcagaacttc tgaggcggaa agaaccagct ggggctctag
     3361 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     3421 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     3481 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     3541 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     3601 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     3661 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     3721 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     3781 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     3841 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     3901 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     3961 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     4021 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     4081 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     4141 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacat gaccgagtac
     4201 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     4261 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     4321 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     4381 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     4441 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     4501 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     4561 ctggccaccg tcggagtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     4621 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     4681 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     4741 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagt gctacgagat
     4801 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     4861 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     4921 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     4981 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     5041 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     5101 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     5161 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     5221 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     5281 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     5341 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     5401 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     5461 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5521 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5581 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     5641 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     5701 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     5761 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     5821 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tataggcggt
     5881 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     5941 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     6001 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6061 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6121 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     6181 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     6241 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     6301 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     6361 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     6421 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     6481 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     6541 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     6601 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     6661 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     6721 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     6781 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     6841 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     6901 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     6961 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     7021 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7081 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     7141 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     7201 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
     7261 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     7321 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
     7381 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
     7441 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
     7501 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     7561 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     7621 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     7681 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     7741 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     7801 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     7861 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     7921 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     7981 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     8041 aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg
     8101 aaattaatac gactcactat agggagaccc aagctggcta gcaccatggc cctgtcccgt
     8161 gcggtttgtg gcacctcccg tcaactggct ccggttctgg gttatctggg ttcccgtcaa
     8221 aaacactccc tgccggacta cccgtatgat gttccggatt acgctggcta cccatacgac
     8281 gtcccagact acgctggcta cccatacgac gtcccagact acgctatgga catcgcggat
     8341 ctgcgtaccc tgggttacag ccagcagcag caggagaaga tcaagccgaa ggtgcgcagc
     8401 accgtggctc agcaccacga agccctggtg ggccacggtt tcacccacgc tcacattgtg
     8461 gccctgagcc agcacccagc cgcgctgggc accgtggccg tgaaatatca ggatatgatt
     8521 gctgccctgc cagaggccac ccatgaagct attgtgggcg tgggcaagca gtggagcggt
     8581 gctcgtgcgc tggaggcgct gctgaccgtg gctggtgaac tgcgtgg
//
