LOCUS       PB-mCherry-DMDEx        7632 bp DNA     circular SYN 28-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 29, 2023 from SnapGene Viewer 6.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    27..1304
                     /label=mCherry-DMDEx23
     regulatory      1312..1321
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1318..2037
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    2189..2244
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2969..2985)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2993..3009
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3017..3047)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3062..3083
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3142..3337
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3188..3323
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3343..3477
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3716..4304)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4475..5335)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5336..5440)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    complement(5837..5870)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     enhancer        5966..6269
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6272..6547
                     /label=chicken beta-actin promoter
     intron          6548..7556
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
ORIGIN
        1 ccggggactc agatctcgag gccaccatgg tgagcaaggg cgaggaggat aacatggcca
       61 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
      121 tcgagatcga ggtaagggca ctgagcagaa gggaagaagc tccgggggct ctttgtaggg
      181 tcctccagtc aggactcaaa cccagtagtg tctggttcca ggcactgacc ttgtatgtct
      241 cctggcccaa atgcccactc agggtagggg tgtagggcag acaacgagtc ttttgtcatc
      301 tacaggctct gcaaagttct ttgaaagagc aataaaatgg cttcaactat ctgagtgaca
      361 ctgtgaagga gatggccaag aaagcacctt cagaaatatg ccagaaatat ctgtcagaat
      421 ttgaagagat tgaggggcac tggaagaaac tttcctccca gttggtggaa agctgccaaa
      481 agctagaaga acatatgaat aaacttcgaa aatttcaggt aagccgaggt ttggccttgg
      541 aagaagctcc gggggctctt tgtagggtcc tccagtcagg actcaaaccc agtagtgtct
      601 ggttccaggc actgaccttg tatgtctcct ggcccaaatg cccactcagg gtaggggtgt
      661 agggcagaca acgagtcttt tgtcatctac agggcgaggg cgagggccgc ccctacgagg
      721 gcacccagac cgccaagctg gaggtgacca agggtggccc cctgcccttc gcctgggaca
      781 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
      841 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
      901 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
      961 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
     1021 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     1081 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
     1141 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
     1201 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     1261 gccactccac cggcggcatg gacgagctgt acaagtaagg atccaccggt cgccaccatg
     1321 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     1381 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     1441 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     1501 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     1561 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     1621 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     1681 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     1741 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     1801 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     1861 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     1921 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     1981 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc
     2041 ggccgcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc
     2101 ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg
     2161 aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg
     2221 tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa
     2281 catcagaatg agtatttggt ttagagtttg gcaacatatg ccatatgctg gctgccatga
     2341 acaaaggtgg ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt
     2401 attccataga aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt
     2461 tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct
     2521 ctcctgacta ctcccagtca tagctgtccc tcttctctta tgaagatccc tcgacctgca
     2581 ggctccagcc tacacaatcg ctcaagacgt gtaatgcttt tattatatat tagtcacgat
     2641 atctataaca agaaaatata tatataataa gttatcacgt aagtagaaca tgaaataaca
     2701 atataattat cgtatgagtt aaatcttaaa agtcacgtaa aagataatca tgcgtcattt
     2761 tgactcacgc ggtcgttata gttcaaaatc agtgacactt accgcattga caagcacgcc
     2821 tcacgggagc tccaagcggc gactgagatg tcctaaatgc acagcgacgg attcgcgcta
     2881 tttagaaaga gagagcaata tttcaagaat gcatgcgtca attttacgca gactatcttt
     2941 ctagggttaa aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     3001 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     3061 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     3121 acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac
     3181 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     3241 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     3301 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct
     3361 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     3421 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgc
     3481 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     3541 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     3601 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     3661 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     3721 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     3781 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     3841 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     3901 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     3961 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     4021 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     4081 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     4141 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     4201 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     4261 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     4321 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     4381 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     4441 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     4501 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     4561 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     4621 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     4681 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     4741 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     4801 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     4861 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     4921 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     4981 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     5041 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     5101 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     5161 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     5221 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     5281 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     5341 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     5401 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     5461 cacctgggtc gacttaaccc tagaaagata atcatattgt gacgtacgtt aaagataatc
     5521 atgcgtaaaa ttgacgcatg tgttttatcg atctgtatat cgaggtttat ttattaattt
     5581 gaatagatat taagttttat tatatttaca cttacatact aataataaat tcaacaaaca
     5641 atttatttat gtttatttat ttattaaaaa aaaacaaaaa ctcaaaattt cttctataaa
     5701 gtaacaaaac ttttaaacat tctctctttt acaaaaataa acttattttg tactttaaaa
     5761 acagtcatgt tgtattataa aataagtaat tagcttaact tatacataat agaaacaaat
     5821 tatacttatt aatcgcataa cttcgtataa tgtatgctat acgaagttat gcgattaagg
     5881 gatctgtagg gcgcagtagt ccaactagtt attaatagta atcaattacg gggtcattag
     5941 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     6001 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     6061 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg
     6121 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     6181 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     6241 tctacgtatt agtcatcgct attaccatgg gtcgaggtga gccccacgtt ctgcttcact
     6301 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
     6361 tgtgcagcga tgggggcggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga
     6421 ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg
     6481 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
     6541 gcgggcggga gtcgctgcgt tgccttcgcc ccgtgccccg ctccgcgccg cctcgcgccg
     6601 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
     6661 tcctccgggc tgtaattagc gcttggttta atgacggctc gtttcttttc tgtggctgcg
     6721 tgaaagcctt aaagggctcc gggagggccc tttgtgcggg ggggagcggc tcggggggtg
     6781 cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggccc gcgctgcccg gcggctgtga
     6841 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcgtg tgcgcgaggg gagcgcggcc
     6901 gggggcggtg ccccgcggtg cgggggggct gcgaggggaa caaaggctgc gtgcggggtg
     6961 tgtgcgtggg ggggtgagca gggggtgtgg gcgcggcggt cgggctgtaa cccccccctg
     7021 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtgcgggg
     7081 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
     7141 gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc cggagcgccg
     7201 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     7261 cgcagggact tcctttgtcc caaatctggc ggagccgaaa tctgggaggc gccgccgcac
     7321 cccctctagc gggcgcgggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
     7381 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccatc tccagcctcg gggctgccgc
     7441 agggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     7501 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     7561 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc tgcagtcgac
     7621 ggtaccgcgg gc
//
