LOCUS       pcDNA3-ApaLIE225        6702 bp DNA     circular SYN 13-NOV-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6702)
  AUTHORS   Yi Fu
  TITLE     Direct Submission
  JOURNAL   Exported Nov 7, 2023 from SnapGene 7.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6702
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     primer_bind     302..325
                     /label=YF22-SnaBI-CMV-F
                     /note="33	9/26/16	to pcr LacR-11Myc and clone into 
                     pwzl-hygro retroviral construct. But not sure myc repeats 
                     will be stable in viral infected plasmid"
                     /note="color: black; sequence: 
                     cccTACGTAGGAGTTCCGCGTTACATAACTTAC"
     primer_bind     complement(385..406)
                     /label=YF32-CMV-R
                     /note="22"
                     /note="color: black; sequence: GGCGTTACTATGGGAACATACG"
     primer_bind     complement(481..501)
                     /label=YF84_CMVenh_R
                     /note="21"
                     /note="color: black; sequence: GGGGCGTACTTGGCATATGAT"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     complement(791..818)
                     /label=YF24-BioIDstart-ATG-CMV-R
                     /note="51	PCR the CMV enhancer promoter with 
                     CMVenh_XbaI_Fw	do overlap pcr to have 
                     xbai-cmv-startcodon-bioid-ecori and clone into 
                     lenti-tfam-nobioid plasmid"
                     /note="color: black; sequence: 
                     AGCCAGATCAGGTTCTTGAAcatAGCTCTGCTTATATAGACCTCCCACCGT"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             961..1065
                     /codon_start=1
                     /label=Cox8a
                     /note="This feature has 2 segments:
                        1:  961 .. 1060 / #993366
                        2: 1061 .. 1065 / #993366"
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAKIHSLPPEGKL"
     CDS             961..1035
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /note="color: #993366
                     Cleavage site after base 1035"
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     primer_bind     961..977
                     /label=ApaLI_Fw_BsrGI
                     /note="color: black; sequence: atatTGTACAATGTCCGTCCTGACGCC"
     primer_bind     1055..1076
                     /label=YF274-ApaLI-F
                     /note="color: black; sequence: AGGGGAAGCTTATGACCACACG"
     CDS             1066..2190
                     /codon_start=1
                     /label=ApaLI
                     /note="color: #00ccff"
                     /translation="MTTRQRLSAERSQQLTRLLTITKTANMRALMEASELAKVIALVAV
                     DIGKSDEMARAFPVLWPKISPQQEYYATAVDWFTNPDETVTSFDVVDMLDAGTSLDQDF
                     MTYLKCLTELHKRRRKYGLILQRQPLPTMVQVSPRALMEYGPDFPPEALASWLTWRKFF
                     YDLDNRSAQETGYLFEPILAAAIGGEAKSARERVVRRTDDPTKGRQVDCWKVLPDGTPL
                     AYALALRVTIAASGQGRFGEELSFARDCSSSGAKPILVVLDPTENDKLTGLQAAYREVG
                     GAAYVGDAAWAHLEDEAGATMASFIERYVRVPVASVSSFERVIEGDATKRSLILQDLQA
                     RLDGNELTISLGGHQRLVERHEDQSLAADGDDDSE"
     primer_bind     complement(1579..1614)
                     /label=YF275-ApaLICS1-R
                     /note="color: black; sequence: 
                     GGCCAGAATGGGcgcGAACAAGTACCCTGTTTCCTG"
     CDS             1588..1590
                     /codon_start=1
                     /label=type2conserved
                     /note="color: #993366"
                     /translation="G"
     primer_bind     1591..1621
                     /label=YF276-ApaLICS1-F
                     /note="color: black; sequence: 
                     TACTTGTTCGCGCCCATTCTGGCCGCCGCCA"
     CDS             1597..1602
                     /codon_start=1
                     /label=type2conserved
                     /note="color: #993366"
                     /translation="FE"
     CDS             1600..1602
                     /codon_start=1
                     /label=CS1
                     /note="color: #ff00ff"
                     /translation="E"
     CDS             1609..1611
                     /codon_start=1
                     /label=type2conserved
                     /note="color: #993366"
                     /translation="L"
     primer_bind     complement(1678..1708)
                     /label=YF290-ApaLICS2-R2
                     /note="color: black; sequence: 
                     CCTTCCAGCAggcCACCTGCCTGCCCTTCGT"
     primer_bind     complement(1678..1708)
                     /label=YF278-ApaLICS2-R
                     /note="color: black; sequence: 
                     CCTTCCAGCAcgcCACCTGCCTGCCCTTCGT"
     primer_bind     1688..1718
                     /label=YF291-ApaLICS2-F2
                     /note="color: black; sequence: 
                     GGCAGGTGgccTGCTGGAAGGTGCTCCCTGA"
     primer_bind     1688..1718
                     /label=YF279-ApaLICS2-F
                     /note="color: black; sequence: 
                     GGCAGGTGgcgTGCTGGAAGGTGCTCCCTGA"
     CDS             1696..1698
                     /codon_start=1
                     /label=CS2
                     /note="color: #ff00ff"
                     /translation="D"
     CDS             1696..1698
                     /codon_start=1
                     /label=type2conserved
                     /note="color: #993366"
                     /translation="D"
     primer_bind     complement(1718..1756)
                     /label=YF284-ApaLICS34-R
                     /note="color: black; sequence: 
                     tcACCCGAAGcgcCAAcgcATAAGCCAGAGGCGTACCAT"
     primer_bind     complement(1719..1754)
                     /label=YF280-ApaLICS3-R
                     /note="color: black; sequence: 
                     ACCCGAAGTTTCAAcgcATAAGCCAGAGGCGTACCA"
     primer_bind     complement(1723..1757)
                     /label=YF282-ApaLICS4-R
                     /note="color: black; sequence: 
                     GTCACCCGAAGcgcCAATTCATAAGCCAGAGGCGT"
     primer_bind     1726..1763
                     /label=YF281-ApaLICS3-F
                     /note="color: black; sequence: 
                     CCTCTGGCTTATgcgTTGAAACTTCGGGTGACAATTGC"
     primer_bind     1728..1768
                     /label=YF285-ApaLICS34-F
                     /note="color: black; sequence: 
                     TCTGGCTTATgcgTTGgcgCTTCGGGTGACAATTGCAGCGA"
     primer_bind     1732..1768
                     /label=YF283-ApaLICS4-F
                     /note="color: black; sequence: 
                     GCTTATGAATTGgcgCTTCGGGTGACAATTGCAGCGA"
     CDS             1738
                     /codon_start=1
                     /label=CS3
                     /note="color: #ff00ff"
                     /translation=""
     CDS             1738
                     /codon_start=1
                     /label=type2conserved
                     /note="color: #993366"
                     /translation=""
     CDS             1792..1794
                     /codon_start=1
                     /label=type2conserved
                     /note="color: #993366"
                     /translation="E"
     CDS             1840..1848
                     /codon_start=1
                     /label=type2conserved
                     /note="color: #993366"
                     /translation="LVV"
     CDS             2194..2220
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     promoter        complement(2254..2272)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(2288..2307)
                     /label=YF277-ApaLI_R
                     /note="color: black; sequence: GAAGGCACAGTCGAGGCTGA"
     polyA_signal    2298..2522
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(2504..2524)
                     /label=ApaLI_Rv_XhoI
                     /note="color: black; sequence: 
                     atatCTCGAGCCATAGAGCCCACCGCATC"
     primer_bind     complement(2504..2522)
                     /label=YF238-SpeI-bGHpA-R
                     /note="color: black; sequence: 
                     cccACTAGTCCATAGAGCCCACCGCATC"
     rep_origin      2568..2996
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        3010..3340
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      3191..3326
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             3407..4201
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     4168..4188
                     /label=YF100_PacILacO_F
                     /note="32	""pcr xq-neo-18laco-pal-ef1agfp with YF87, 
                     (blunt) digest with pacI; ligate to pacI+pmeI digested 
                     phageMCSIRESzsG --> test gfp expression in cell"""
                     /note="color: black; sequence: 
                     tcgTTAATTAACGCCTTCTATCGCCTTCTTGA"
     polyA_signal    4375..4496
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(4403..4433)
                     /label=YF202-SV40pA_R
                     /note="pcr with yf197 to make point mut of gfp1-10 ligate"
                     /note="color: black; sequence: 
                     gtgaaatttgtgatgctattgctttatttgt"
     primer_bind     complement(4545..4561)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    4569..4585
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(4593..4623)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 4593 .. 4599 / #ffffff / -10
                        2: 4600 .. 4617 / #ffffff
                        3: 4618 .. 4623 / #ffffff / -35"
     protein_bind    4638..4659
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(4947..5535)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(5706..6566)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 5706 .. 6497 / #ccffcc
                        2: 6498 .. 6566 / #ccffcc / signal sequence
                     Cleavage site after base 6497"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6567..6671)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gtttggtacc
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgggtact ccgtgccatc
      961 atgtccgtcc tgacgccgct gctgctgcgg ggcttgacag gctcggcccg gcggctccca
     1021 gtgccgcgcg ccaagatcca ttcgttgccg ccggagggga agcttatgac cacacgacag
     1081 cgcttatctg ctgagagatc tcagcagctt acccgtctgc tcactatcac taaaaccgct
     1141 aacatgcggg ccttaatgga ggctagcgaa ctcgctaaag taattgccct ggtggcggtt
     1201 gacataggta agtcagacga gatggccaga gctttccccg tgctgtggcc aaaaatcagt
     1261 cctcagcaag aatattacgc aacagcggtt gactggttca ccaaccccga tgaaaccgtc
     1321 acctctttcg atgtggtgga tatgctagat gccggcactt ccctggacca agacttcatg
     1381 acttatctga aatgtctaac tgaactgcac aagcgacgga gaaaatacgg cctgatcctg
     1441 cagaggcagc ctctaccaac gatggtgcag gtttcgcccc gagcactgat ggaatacgga
     1501 cctgactttc cgcccgaggc actggctagc tggcttacct ggcggaagtt tttttacgac
     1561 cttgacaaca ggtctgccca ggaaacaggg tacttgttcg aacccattct ggccgccgcc
     1621 atcggaggcg aggcaaaatc tgccagagag cgtgtcgtaa gacgcaccga cgatccaacg
     1681 aagggcaggc aggtggattg ctggaaggtg ctccctgatg gtacgcctct ggcttatgcg
     1741 ttggcgcttc gggtgacaat tgcagcgagc gggcaaggca gatttggaga ggagctgagc
     1801 ttcgctcggg actgcagcag ctccggcgct aagccgatcc tcgtggtcct ggatccaaca
     1861 gaaaatgaca agctcactgg attgcaagcc gcataccgcg aggttggagg cgccgcatat
     1921 gtgggggatg ccgcctgggc tcacttagag gacgaggctg gtgctaccat ggccagtttt
     1981 atagagcgct atgtcagggt gccagtagca tcagtcagtt ccttcgaaag ggtcattgag
     2041 ggagatgcca caaagcgctc cttgatcctg caggacctcc aggcccgact cgatggtaat
     2101 gagcttacaa tctccctggg aggccatcag cggttggtgg aaaggcatga ggaccagagt
     2161 ctcgcagcgg acggcgatga cgattcagag ctctacccct atgatgtgcc agattacgcc
     2221 tgagctctga gcggccgctc tagagggccc tattctatag tgtcacctaa atgctagagc
     2281 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     2341 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     2401 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     2461 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     2521 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     2581 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     2641 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     2701 tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca
     2761 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     2821 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     2881 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg
     2941 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     3001 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag
     3061 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     3121 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     3181 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     3241 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     3301 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     3361 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     3421 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     3481 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     3541 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     3601 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     3661 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     3721 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     3781 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     3841 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     3901 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     3961 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     4021 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     4081 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     4141 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     4201 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     4261 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     4321 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     4381 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     4441 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     4501 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     4561 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     4621 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     4681 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     4741 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     4801 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     4861 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     4921 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     4981 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5041 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     5101 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
     5161 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     5221 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     5281 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     5341 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     5401 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     5461 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     5521 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     5581 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     5641 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     5701 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     5761 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     5821 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     5881 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     5941 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     6001 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     6061 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     6121 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     6181 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     6241 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     6301 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     6361 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     6421 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     6481 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     6541 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     6601 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     6661 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
