LOCUS       Exported               11626 bp ds-DNA     circular SYN 05-AUG-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Nov 8, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11626
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    233..350
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     277..307
                     /label=YF95_cPPT_seq_F
                     /note="31"
     primer_bind     366..389
                     /label=YF43-CMVenhseq_F
                     /note="24"
     primer_bind     complement(396..422)
                     /label=YF326-KpnI-EcoRI-as
                     /note="27"
     primer_bind     400..418
                     /label=YF325-KpnI-EcoRI-s
                     /note="19	""anneal, insert to kpni+ecori digested tlvc2 
                     tomake nosgRNA"""
     promoter        448..762
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    456..474
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    492..510
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    527..545
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    563..581
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    599..617
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    634..652
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    670..688
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     700..709
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(711..738)
                     /label=YF24-BioIDstart-ATG-CMV-R
                     /note="51	PCR the CMV enhancer promoter with 
                     CMVenh_XbaI_Fw	do overlap pcr to have 
                     xbai-cmv-startcodon-bioid-ecori and clone into 
                     lenti-tfam-nobioid plasmid"
     primer_bind     735..759
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     750..770
                     /label=YF447-tightTREp-F
     primer_bind     770..793
                     /label=YF305-AgeI-COX8MLS-F
     CDS             777..881
                     /codon_start=1
                     /label=Cox8a
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAKIHSLPPEGKL"
     CDS             777..851
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     primer_bind     777..793
                     /label=ApaLI_Fw_BsrGI
     primer_bind     777..793
                     /label=YF355-SalI-COX8MLS-F
     primer_bind     871..892
                     /label=YF274-ApaLI-F
     CDS             882..2006
                     /codon_start=1
                     /label=ApaLI
                     /translation="MTTRQRLSAERSQQLTRLLTITKTANMRALMEASELAKVIALVAV
                     DIGKSDEMARAFPVLWPKISPQQEYYATAVDWFTNPDETVTSFDVVDMLDAGTSLDQDF
                     MTYLKCLTELHKRRRKYGLILQRQPLPTMVQVSPRALMEYGPDFPPEALASWLTWRKFF
                     YDLDNRSAQETGYLFEPILAAAIGGEAKSARERVVRRTDDPTKGRQVDCWKVLPDGTPL
                     AYELKLRVTIAASGQGRFGEELSFARDCSSSGAKPILVVLDPTENDKLTGLQAAYREVG
                     GAAYVGDAAWAHLEDEAGATMASFIERYVRVPVASVSSFERVIEGDATKRSLILQDLQA
                     RLDGNELTISLGGHQRLVERHEDQSLAADGDDDSE"
     primer_bind     complement(1395..1430)
                     /label=YF275-ApaLICS1-R
     CDS             1404..1406
                     /codon_start=1
                     /label=type2conserved
                     /translation="G"
     primer_bind     1407..1437
                     /label=YF276-ApaLICS1-F
     CDS             1413..1418
                     /codon_start=1
                     /label=type2conserved
                     /translation="FE"
     CDS             1416..1418
                     /codon_start=1
                     /label=CS1
                     /translation="E"
     CDS             1425..1427
                     /codon_start=1
                     /label=type2conserved
                     /translation="L"
     primer_bind     complement(1494..1524)
                     /label=YF278-ApaLICS2-R
     primer_bind     complement(1494..1524)
                     /label=YF290-ApaLICS2-R2
     primer_bind     1504..1534
                     /label=YF279-ApaLICS2-F
     primer_bind     1504..1534
                     /label=YF291-ApaLICS2-F2
     CDS             1512..1514
                     /codon_start=1
                     /label=type2conserved
                     /translation="D"
     CDS             1512..1514
                     /codon_start=1
                     /label=CS2
                     /translation="D"
     primer_bind     complement(1534..1572)
                     /label=YF284-ApaLICS34-R
     primer_bind     complement(1535..1570)
                     /label=YF280-ApaLICS3-R
     primer_bind     complement(1539..1573)
                     /label=YF282-ApaLICS4-R
     primer_bind     1542..1579
                     /label=YF281-ApaLICS3-F
     primer_bind     1544..1584
                     /label=YF285-ApaLICS34-F
     primer_bind     1548..1584
                     /label=YF283-ApaLICS4-F
     CDS             1554..1562
                     /codon_start=1
                     /label=type2conserved
                     /translation="ELK"
     CDS             1554..1556
                     /codon_start=1
                     /label=CS3
                     /translation="E"
     CDS             1560..1562
                     /codon_start=1
                     /label=CS4
                     /translation="K"
     CDS             1608..1610
                     /codon_start=1
                     /label=type2conserved
                     /translation="E"
     CDS             1656..1664
                     /codon_start=1
                     /label=type2conserved
                     /translation="LVV"
     primer_bind     complement(1990..2033)
                     /label=YF335-ApaLI-FLAGSTOP-R
     primer_bind     complement(2007..2041)
                     /label=YF446-AscI-FLAG-R
     CDS             2010..2033
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(2015..2033)
                     /label=YF382-SbfI-FLAG-R
                     /note="36	""pcr aavs-tre3gapali to get tre3g-apali-flag 
                     1717bp (59c), cut with sbf1+asci, ligate to 
                     phage-cmv-teton3g"""
     primer_bind     complement(2015..2033)
                     /label=YF388-NsiIFLAG-R
                     /note="35	""pcr with YF383- toget 1737 60C, cut with NsiI +
                     AscI, ligate to SbfI+AscI cut phage-cmv-teton3g-ires-puro 
                     plasmid"""
     primer_bind     complement(2016..2033)
                     /label=YF356-MluI-FLAG-R
     primer_bind     2034..2043
                     /label=YF329-AgeI-BstXI-AscI-BamHI-s
                     /note="41	""anneal, ligate to agei+bamHI digested 
                     tlvc2-nosgRNA or tlvc2"""
     primer_bind     2043..2066
                     /label=YF572-BamHI-T2A-F
     primer_bind     2047..2132
                     /label=Primer 1
     CDS             2058..2111
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2112..2825
                     /codon_start=1
                     /label=mTagBFP2
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     primer_bind     2112..2156
                     /label=STB-mTagBFP2-For
     primer_bind     2112..2132
                     /label=mTagBFP2-OL-For
     primer_bind     complement(2113..2132)
                     /label=YF349-tdTomatostart-R
                     /note="20"
     primer_bind     2628..2659
                     /label=STB-BFP-I174A-For
     primer_bind     complement(2628..2659)
                     /label=STB-BFP-I174A-Rev
     primer_bind     complement(2800..2825)
                     /label=mTagBFP2-ClaI-Rev
     primer_bind     complement(2804..2846)
                     /label=YF573-BmtIBstXI-mTagBFP2-R
     primer_bind     2836..2900
                     /label=YF180-IsceIphiKO2EF1aMfeI-s
                     /note="188"
     promoter        2848..3059
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     primer_bind     complement(2948..2968)
                     /label=YF80_EF1a_R
                     /note="21"
     CDS             3081..3677
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3081..3100)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3081..3098
                     /label=YF105_MluIPuro_F
                     /note="30	""pcr puro from plpcFlagTelA, then cut with mlui,
                     and inserted into mlui cut aavs1-CMVFLagTela"""
     primer_bind     3081..3095
                     /label=YF7_NotI-Puro_Fwd
                     /note="33	8/18/16	65	PCR puro from pBABE puroCre-ERT2 and 
                     insert into NotI site of XpYpCC1PAL22LacO"
     primer_bind     complement(3523..3533)
                     /label=YF276-ApaLICS1-F
     primer_bind     3577..3597
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3687..3743
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3744..4490
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4469..4490)
                     /label=YF381-SphI-TetOn3G-R
                     /note="31"
     misc_feature    4511..5099
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4564..4584)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4982..4993)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5066..5084
                     /label=YF131_WPREend_F
                     /note="19"
     LTR             5171..5404
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5430..5447)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5436..5660
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5532..5551)
                     /label=YF457-bGHpolyA-R
     rep_origin      5706..6134
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5793..5812)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6003..6024
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6143..6163)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6148..6477
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6328..6463
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6390..6409
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6525..6572
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6591..6965
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7095..7216
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7123..7153)
                     /label=YF202-SV40pA_R
                     /note="31	pcr with yf197 to make point mut of gfp1-10 
                     ligate"
     primer_bind     complement(7132..7151)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7186..7205
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7265..7281)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7265..7281)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7278..7300)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7289..7305
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7313..7343)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7358..7379
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     7376..7395
                     /label=YF370-AAVS1R-F
                     /note="20	use with YF129 for right arm genotyping"
     primer_bind     complement(7496..7513)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7667..8255)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7747..7766)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8426..9286)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9049..9068
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9287..9391)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9466..9485)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9651..9675
                     /label=YF386-HindIIICMV-F
                     /note="34	""pcr from psl301 cmv, cut with hind + mlui, 
                     ligate to psl301-13gfpHygro"""
     enhancer        9657..10036
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(9694..9717)
                     /label=YF383-AscI-F
                     /note="35"
     primer_bind     9723..9749
                     /label=YF380-SpeI-CMV-F
                     /note="35	""pcr aavs-tre3gapali to get teton3g 2420bp 61C, 
                     cut with spei and sphi, ligate to phageflagtelkirespuro 
                     spei+sphi""	""didn't work well with kod, ladderingE """
     primer_bind     9724..9747
                     /label=YF22-SnaBI-CMV-F
                     /note="33	9/26/16	to pcr LacR-11Myc and clone into 
                     pwzl-hygro retroviral construct. But not sure myc repeats 
                     will be stable in viral infected plasmid"
     primer_bind     complement(9807..9828)
                     /label=YF32-CMV-R
                     /note="22"
     primer_bind     complement(9903..9923)
                     /label=YF84_CMVenh_R
                     /note="21"
     promoter        10037..10240
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        10038..10236
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10078..10097
                     /label=YF412-CMVPURO-F1
     primer_bind     10191..10211
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(10213..10239)
                     /label=YF24-BioIDstart-ATG-CMV-R
                     /note="51	PCR the CMV enhancer promoter with 
                     CMVenh_XbaI_Fw	do overlap pcr to have 
                     xbai-cmv-startcodon-bioid-ecori and clone into 
                     lenti-tfam-nobioid plasmid"
     LTR             10254..10434
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10481..10606
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11099..11332
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
       61 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
      121 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
      181 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
      241 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
      301 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
      361 acagggacag cagagatcca gtttggttaa ttaaggtacc gagggcctat ttcttttgaa
      421 ttcactttgg ccgcgaatcg atatgtcgag tttactccct atcagtgata gagaacgtat
      481 gtcgagttta ctccctatca gtgatagaga acgatgtcga gtttactccc tatcagtgat
      541 agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc
      601 cctatcagtg atagagaacg tatgtcgagt ttatccctat cagtgataga gaacgtatgt
      661 cgagtttact ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt acggtgggag
      721 gcctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagaat accggtatgt
      781 ccgtcctgac gccgctgctg ctgcggggct tgacaggctc ggcccggcgg ctcccagtgc
      841 cgcgcgccaa gatccattcg ttgccgccgg aggggaagct tatgaccaca cgacagcgct
      901 tatctgctga gagatctcag cagcttaccc gtctgctcac tatcactaaa accgctaaca
      961 tgcgggcctt aatggaggct agcgaactcg ctaaagtaat tgccctggtg gcggttgaca
     1021 taggtaagtc agacgagatg gccagagctt tccccgtgct gtggccaaaa atcagtcctc
     1081 agcaagaata ttacgcaaca gcggttgact ggttcaccaa ccccgatgaa accgtcacct
     1141 ctttcgatgt ggtggatatg ctagatgccg gcacttccct ggaccaagac ttcatgactt
     1201 atctgaaatg tctaactgaa ctgcacaagc gacggagaaa atacggcctg atcctgcaga
     1261 ggcagcctct accaacgatg gtgcaggttt cgccccgagc actgatggaa tacggacctg
     1321 actttccgcc cgaggcactg gctagctggc ttacctggcg gaagtttttt tacgaccttg
     1381 acaacaggtc tgcccaggaa acagggtact tgttcgaacc cattctggcc gccgccatcg
     1441 gaggcgaggc aaaatctgcc agagagcgtg tcgtaagacg caccgacgat ccaacgaagg
     1501 gcaggcaggt ggattgctgg aaggtgctcc ctgatggtac gcctctggct tatgaattga
     1561 aacttcgggt gacaattgca gcgagcgggc aaggcagatt tggagaggag ctgagcttcg
     1621 ctcgggactg cagcagctcc ggcgctaagc cgatcctcgt ggtcctggat ccaacagaaa
     1681 atgacaagct cactggattg caagccgcat accgcgaggt tggaggcgcc gcatatgtgg
     1741 gggatgccgc ctgggctcac ttagaggacg aggctggtgc taccatggcc agttttatag
     1801 agcgctatgt cagggtgcca gtagcatcag tcagttcctt cgaaagggtc attgagggag
     1861 atgccacaaa gcgctccttg atcctgcagg acctccaggc ccgactcgat ggtaatgagc
     1921 ttacaatctc cctgggaggc catcagcggt tggtggaaag gcatgaggac cagagtctcg
     1981 cagcggacgg cgatgacgat tcagagctcg attacaaaga cgatgacgat aagggcgcgc
     2041 ctggatccgg cagtggagag ggcagaggaa gtctgctaac atgcggtgac gtcgaggaga
     2101 atcctggccc aatggtgagc aagggtgagg agctgattaa ggagaacatg cacatgaagc
     2161 tgtacatgga gggcaccgtg gacaaccatc acttcaagtg cacatccgag ggcgaaggca
     2221 agccctacga gggcacccag accatgagaa tcaaggtggt cgagggcggc cctctcccct
     2281 tcgccttcga catcctggct actagcttcc tctacggcag caagaccttc atcaaccaca
     2341 cccagggcat ccccgacttc ttcaagcagt ccttccctga gggcttcaca tgggagagag
     2401 tcaccacata cgaagacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg
     2461 gctgcctcat ctacaacgtc aagatcagag gggtgaactt cacatccaac ggccctgtga
     2521 tgcagaagaa aacactcggc tgggaggcct tcaccgagac gctgtacccc gctgacggcg
     2581 gcctggaagg cagaaacgac atggccctga agctcgtggg cgggagccat ctgatcgcaa
     2641 acgccaagac cacatataga tccaagaaac ccgctaagaa cctcaagatg cctggcgtct
     2701 actatgtgga ctacagactg gaaagaatca aggaggccaa caacgagacc tacgtcgagc
     2761 agcacgaggt ggcagtggcc agatactgcg acctccctag caaactgggg cacaagctta
     2821 attgaccatt cgattgggct agcatccggg cagagcgcac atcgcccaca gtccccgaga
     2881 agttgggggg aggggtcggc aattgatccg gtgcctagag aaggtggcgc ggggtaaact
     2941 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     3001 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacaga
     3061 ctgcgatcgc aatgtacagt atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     3121 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     3181 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     3241 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     3301 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     3361 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     3421 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggagtc tcgcccgacc
     3481 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     3541 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     3601 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     3661 cccgcaagcc cggtgccggt tccggcgcaa caaacttctc tctgctgaaa caagccggag
     3721 atgtcgaaga gaatcctgga ccgatgtcta gactggacaa gagcaaagtc ataaacggag
     3781 ctctggaatt actcaatggt gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa
     3841 agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg
     3901 atgccctgcc aatcgagatg ctggacaggc atcataccca cttctgcccc ctggaaggcg
     3961 agtcatggca agactttctg cggaacaacg ccaagtcata ccgctgtgct ctcctctcac
     4021 atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc
     4081 tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg
     4141 ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggaacag gagcatcaag
     4201 tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt ctgagacaag
     4261 caattgagct gttcgaccgg cagggagccg aacctgcctt ccttttcggc ctggaactaa
     4321 tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc gacgcccttg
     4381 acgattttga cttagacatg ctcccagccg atgcccttga cgactttgac cttgatatgc
     4441 tgcctgctga cgctcttgac gattttgacc ttgacatgct ccccgggtaa ctcgagcgcg
     4501 ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     4561 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     4621 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     4681 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     4741 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     4801 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     4861 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     4921 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     4981 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     5041 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg
     5101 tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     5161 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     5221 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     5281 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     5341 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     5401 agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     5461 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     5521 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     5581 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     5641 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     5701 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     5761 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     5821 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     5881 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     5941 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     6001 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     6061 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     6121 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     6181 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     6241 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     6301 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     6361 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     6421 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     6481 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat
     6541 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt
     6601 tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga
     6661 ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg
     6721 acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg
     6781 cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca
     6841 cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc
     6901 gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg
     6961 actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     7021 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     7081 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7141 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7201 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     7261 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     7321 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     7381 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     7441 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     7501 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     7561 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     7621 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     7681 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     7741 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     7801 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     7861 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     7921 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     7981 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8041 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8101 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8161 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8221 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8281 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     8341 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     8401 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     8461 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     8521 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     8581 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     8641 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     8701 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     8761 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     8821 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     8881 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     8941 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9001 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     9061 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     9121 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     9181 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     9241 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     9301 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     9361 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     9421 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
     9481 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
     9541 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
     9601 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
     9661 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     9721 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     9781 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     9841 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     9901 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     9961 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
    10021 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
    10081 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
    10141 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
    10201 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
    10261 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
    10321 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
    10381 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
    10441 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
    10501 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
    10561 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
    10621 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
    10681 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
    10741 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
    10801 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
    10861 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
    10921 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
    10981 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
    11041 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
    11101 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
    11161 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
    11221 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
    11281 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
    11341 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
    11401 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
    11461 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
    11521 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
    11581 caaattggct gtggtatata aaattattca taatgatagt aggagg
//
