LOCUS       pAS088_-_U6-BsmBI-Stuffer-BsmBI_I-CeuI_gRNABB(SapI)-_Cbh_-_Cre_-_HA_-_T2A_-_SBP_-_EGFP_-_KASH(BsmBIfree)_-_WPRE-shortPA-BsmBIfreebb        7452 bp    DNA     circular UNA 28-FEB-2022
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...al-agswosj
VERSION     urn.local...al-agswosj
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     gene            1..141
                     /gene="L-ITR"
                     /standard_name="L-ITR"
     misc_feature    join(<4029..5368,<878..>1710,1..>154)
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of
                     pCM198_pAAV-U6(SapI-SapI)Cbh-mCherryHA-WPRE-shpA -
                     Fragment 2 extraction (concatenated sequence 1)"
     misc_feature    1..2799
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pEG043_AUTO extraction (concatenated
                     sequence 1)"
     misc_feature    join(1..>154,<878..2799)
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pEG003 -
                     U6-stuffer-gRNAbackbone template - Fragment 2 extraction
                     (concatenated sequence 1)"
     misc_feature    join(1..>154,<878..1710)
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pCM210 -
                     pAAV-U6(SapI-SapI)-CbH-mCherryHA-WPRE-shpA-BsmBIfree -
                     Fragment 2 - Digestion of
                     pCM198_pAAV-U6(SapI-SapI)Cbh-mCherryHA-WPRE-shpA -
                     Fragment 2 extraction (concatenated sequence 1)
                     (concatenated sequence 1)"
     misc_feature    1..154
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pEG29 - Fragment 2 -
                     Digestion of pCM210 -
                     pAAV-U6(SapI-SapI)-CbH-mCherryHA-WPRE-shpA-BsmBIfree -
                     Fragment 2 - Digestion of
                     pCM198_pAAV-U6(SapI-SapI)Cbh-mCherryHA-WPRE-shpA -
                     Fragment 2 extraction (concatenated sequence 1)
                     (concatenated sequence 1) (concatenated sequence 1)"
     misc_feature    150..>154
                     /note="MluI"
                     /standard_name="MluI"
     misc_feature    151..154
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    <151..155
                     /note="MluI"
                     /standard_name="MluI"
     misc_feature    151..881
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pEG013 - Fragment 1
                     (concatenated sequence 2)"
     misc_feature    join(<151..>336,<878..>881)
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pCM66_pAAV-hSyn - Fragment 2
                     (concatenated sequence 1)"
     misc_feature    join(<151..>336,<878..>881)
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pEG006-
                     pAAV-U6-Stuffer_I-CeuI_gRNABB(SapI)-hSyn-Cre-HA-T2A-SBP-EG
                     FP-KASH-WPRE-shortPA-BsmBIfreebb - Fragment 2 extraction
                     (concatenated sequence 1)"
     misc_feature    join(<151..>336,<878..>881)
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pEG001 - Fragment 2 -
                     Digestion of pCM200 -
                     pAAV-U6-gRNABB(SapI)-hSyn-Cre-HA-T2A-EGFP-KASH-WPRE-shortP
                     A-BsmBIfreebb - Fragment 2 - Digestion of pCM66_pAAV-hSyn
                     - Fragment 2 (concatenated sequence 1) (concatenated
                     sequence 1) (concatenated sequence 1)"
     misc_feature    join(<151..>336,<878..>881)
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pCM200 -
                     pAAV-U6-gRNABB(SapI)-hSyn-Cre-HA-T2A-EGFP-KASH-WPRE-shortP
                     A-BsmBIfreebb - Fragment 2 - Digestion of pCM66_pAAV-hSyn
                     - Fragment 2 (concatenated sequence 1) (concatenated
                     sequence 1)"
     misc_feature    <151..336
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pEG012_planed - Fragment 2 -
                     Digestion of pEG002 -
                     pAAV-U6-gRNABB(SapI)-hSyn-Cre-HA-T2A-SBP-EGFP-KASH-WPRE-sh
                     ortPA-BsmBIfreebb - Fragment 2 - Digestion of pCM200 -
                     pAAV-U6-gRNABB(SapI)-hSyn-Cre-HA-T2A-EGFP-KASH-WPRE-shortP
                     A-BsmBIfreebb - Fragment 2 - Digestion of pCM66_pAAV-hSyn
                     - Fragment 2 (concatenated sequence 1) (concatenated
                     sequence 1) (concatenated sequence 1) (concatenated
                     sequence 1)"
     misc_feature    <151..>336
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pEG002 -
                     pAAV-U6-gRNABB(SapI)-hSyn-Cre-HA-T2A-SBP-EGFP-KASH-WPRE-sh
                     ortPA-BsmBIfreebb - Fragment 2 - Digestion of pCM200 -
                     pAAV-U6-gRNABB(SapI)-hSyn-Cre-HA-T2A-EGFP-KASH-WPRE-shortP
                     A-BsmBIfreebb - Fragment 2 - Digestion of pCM66_pAAV-hSyn
                     - Fragment 2 (concatenated sequence 1) (concatenated
                     sequence 1) (concatenated sequence 1)"
     promoter        156..404
     primer_bind     156..177
                     /standard_name="EG082"
     primer_bind     156..175
                     /standard_name="EG010"
     primer_bind     252..278
                     /standard_name="Seq_0059"
     misc_feature    complement(278..301)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="IVS285"
     misc_feature    <335..386
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    335..336
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    335..881
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of gBlock PCR Product - Fragment
                     2 (concatenated sequence 2)"
     primer_bind     345..404
                     /standard_name="EG014"
     primer_bind     348..372
                     /standard_name="IVS039"
     primer_bind     372..404
                     /standard_name="EG044"
     primer_bind     386..404
                     /standard_name="EG040"
     rRNA            387..866
                     /standard_name="gBlock"
     primer_bind     387..406
                     /standard_name="EG024"
     gene            complement(496..>786)
                     /gene="lacZalpha"
                     /standard_name="lacZalpha gene"
     CDS             complement(496..>786)
                     /gene="lacZalpha"
                     /codon_start=1
                     /product="beta-galactosidase alpha fragment"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLN
                     RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTI
                     CSDAA"
                     /standard_name="lacZalpha CDS"
     misc_feature    536..562
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     primer_bind     588..608
                     /standard_name="Seq_0060"
     primer_bind     752..771
                     /standard_name="IVS373_seq_T7_PHP.B_fw"
     misc_feature    773..>786
                     /gene="lacZalpha"
                     /note="multiple cloning site (EcoRI-HindIII)"
                     /note="Geneious type: polylinker"
                     /standard_name="lacZalpha misc"
     misc_feature    <795..>866
                     /note="chimeric RNA backbone 1 (SapI)"
                     /standard_name="chimeric RNA backbone 1 (SapI)"
     misc_feature    complement(795..854)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="EG015"
     misc_feature    complement(827..847)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="EG041"
     misc_feature    complement(842..863)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="IVS040"
     misc_feature    complement(845..867)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="EG045"
     misc_feature    complement(847..866)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="EG025"
     misc_feature    complement(847..866)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="EG083"
     misc_feature    867..>881
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    878..881
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    <878..>881
                     /note="hSyn"
                     /standard_name="hSyn"
     misc_feature    <878..>881
                     /note="XbaI"
                     /standard_name="XbaI"
     misc_feature    join(4696..>5368,<5755..>154,<878..>1710)
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pCM5_pAAV-EF1a-EYFP-W
                     (modified) - Fragment 2 (concatenated sequence 1)"
     misc_feature    join(878..>2799,<4029..7452)
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pEG29 - Fragment 2 extraction
                     (concatenated sequence 3)"
     misc_feature    878..>881
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pEG012_planed - Fragment 2
                     extraction (concatenated sequence 3)"
     misc_feature    <878..>881
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pEG002 -
                     pAAV-U6-gRNABB(SapI)-hSyn-Cre-HA-T2A-SBP-EGFP-KASH-WPRE-sh
                     ortPA-BsmBIfreebb - Fragment 2 extraction (concatenated
                     sequence 3)"
     misc_feature    889..1705
                     /note="CBh"
                     /standard_name="CBh"
     misc_feature    complement(904..924)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="Seq_0045_Cbh_rev_"
     misc_feature    1119
                     /note="A->G mutation"
                     /standard_name="A->G mutation"
     primer_bind     1664..1683
                     /standard_name="Seq_0035_fwd_Cbh"
     misc_feature    1706..>1710
                     /note="AgeI"
                     /standard_name="AgeI"
     misc_feature    <1707..1711
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    1707..1710
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    join(1707..>2799,<4029..4032)
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pEG002 PCR Product - Fragment
                     2 (concatenated sequence 2)"
     misc_feature    1712..1717
                     /standard_name="Kozak"
     misc_feature    join(<4029..>4032,<1712..>2799)
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pCM66_pAAV-hSyn - Fragment 2
                     (concatenated sequence 1)"
     misc_feature    <1712..2799
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pEG001 - Fragment 2 -
                     Digestion of pCM200 -
                     pAAV-U6-gRNABB(SapI)-hSyn-Cre-HA-T2A-EGFP-KASH-WPRE-shortP
                     A-BsmBIfreebb - Fragment 2 - Digestion of pCM66_pAAV-hSyn
                     - Fragment 2 (concatenated sequence 1) (concatenated
                     sequence 1) (concatenated sequence 1)"
     misc_feature    <1712..2799
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pCM200 -
                     pAAV-U6-gRNABB(SapI)-hSyn-Cre-HA-T2A-EGFP-KASH-WPRE-shortP
                     A-BsmBIfreebb - Fragment 2 - Digestion of pCM66_pAAV-hSyn
                     - Fragment 2 (concatenated sequence 1) (concatenated
                     sequence 1)"
     primer_bind     1712..1729
                     /standard_name="ES005_AgeI"
     primer_bind     1712..1729
                     /standard_name="EG005"
     CDS             1718..2767
                     /standard_name="Cre"
     misc_feature    1721..1745
                     /note="Geneious type: PCR_primer"
                     /standard_name="PCR primer"
     primer_bind     1750..1769
                     /standard_name="IVS274"
     primer_bind     1779..1806
                     /standard_name="IVS276"
     misc_feature    complement(1863..1882)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="IVS275"
     primer_bind     2153..2171
                     /standard_name="IVS289"
     primer_bind     2251..2270
                     /standard_name="Seq_0044_fwd_Cre"
     primer_bind     2658..2678
                     /standard_name="IVS290"
     CDS             2768..2794
                     /standard_name="HA"
     misc_feature    <2796..>2799
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    2796..2799
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    2796..4032
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="insert (concatenated sequence 2)"
     misc_feature    2796..>2799
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of addgene_73310_Blasticidin
                     resistance PCR Product - Fragment 2 (concatenated sequence
                     2)"
     misc_feature    2796..>2799
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pCM206 -
                     pAAV-CBh-sfGFP-2A-SBP-Link-KASH-WPRE-bGHpA-BsmBIfreebb PCR
                     Product - Fragment 2 (concatenated sequence 2)"
     misc_feature    2801..2863
                     /note="2A"
                     /note="Geneious type: CDS"
                     /standard_name="2A"
     CDS             2864..2977
                     /standard_name="SBP"
     primer_bind     2867..2887
                     /standard_name="ES003_NheI"
     misc_feature    2902
                     /standard_name="PmlI mod"
     CDS             2978..3022
                     /standard_name="Linker"
     misc_feature    complement(3003..3022)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="ES004"
     gene            3029..3742
                     /gene="EGFP"
                     /note="Geneious type: CDS"
                     /standard_name="EGFP gene"
     primer_bind     3029..3046
                     /standard_name="ES001_NheI"
     misc_feature    complement(3417..3436)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="Seq_0043_rev_GFP"
     primer_bind     3624..3643
                     /standard_name="Seq_0032_fwd_sfGFP"
     CDS             3743..3760
                     /standard_name="SGLRS linker"
     CDS             3761..4006
                     /standard_name="KASH domain of Nesprin2"
     misc_feature    3823
                     /note="silent mutation to remove BsmBI site"
                     /standard_name="misc"
     misc_feature    complement(3993..4010)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="EG023"
     misc_feature    4029..4032
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    4029..7452
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pEG043_AUTO - Digestion of pCM210 -
                     pAAV-U6(SapI-SapI)-CbH-mCherryHA-WPRE-shpA-BsmBIfree -
                     Fragment 2 extraction (concatenated sequence 3)
                     (concatenated sequence 3)"
     misc_feature    <4029..7452
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pEG003 -
                     U6-stuffer-gRNAbackbone template - Fragment 2 extraction
                     (concatenated sequence 3)"
     misc_feature    4029..7452
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pCM210 -
                     pAAV-U6(SapI-SapI)-CbH-mCherryHA-WPRE-shpA-BsmBIfree -
                     Fragment 2 extraction (concatenated sequence 3)"
     misc_feature    <4029..4695
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pCM66_WPRE_shPA (concatenated
                     sequence 2)"
     misc_feature    <4029..>4032
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pEG001 - Fragment 2
                     extraction (concatenated sequence 3)"
     misc_feature    <4029..>4032
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pCM200 -
                     pAAV-U6-gRNABB(SapI)-hSyn-Cre-HA-T2A-EGFP-KASH-WPRE-shortP
                     A-BsmBIfreebb - Fragment 2 extraction (concatenated
                     sequence 3)"
     misc_feature    4030..>4032
                     /note="WPRE"
                     /note="Geneious type: 3'UTR"
                     /standard_name="WPRE"
     misc_feature    4030..4625
                     /note="WPRE"
                     /standard_name="WPRE"
     misc_feature    complement(4035..4059)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="EG006"
     misc_feature    complement(4093..4113)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="Seq_0017_WPRE_rev"
     misc_feature    complement(4115..4134)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="IVS413_Ai14_mut_r"
     primer_bind     4326..4343
                     /standard_name="IVS395_IVS391_ddPCR_WPRE_fw"
     primer_bind     4345..4361
                     /standard_name="IVS397_IVS393_ddPCR_WPRE_Probe"
     misc_feature    complement(4409..4426)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="IVS396_IVS392_ddPCR_WPRE_rv"
     misc_feature    4645..4692
                     /note="ShortPA"
                     /standard_name="ShortPA"
     misc_feature    complement(4689..4707)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="Seq_0029_rev_shpolA"
     gene            4715..4855
                     /gene="R-ITR"
                     /standard_name="R-ITR"
     misc_feature    complement(4865..4885)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="Seq_0060"
     exon            4947..5253
                     /gene="f1 Origin"
                     /standard_name="f1 Origin"
     primer_bind     5227..5247
                     /standard_name="Seq_0025"
     misc_feature    5369..5754
                     /note="Geneious type: sequence variant"
                     /standard_name
     misc_feature    5369..5754
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of gBlock_exBsmBI - Fragment 2
                     (concatenated sequence 2)"
     misc_feature    5755..7452
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of
                     pCM198_pAAV-U6(SapI-SapI)Cbh-mCherryHA-WPRE-shpA -
                     Fragment 2 (concatenated sequence 3)"
     exon            5772..6629
                     /gene="AmpR"
                     /standard_name="AmpR"
     primer_bind     6276..6294
                     /standard_name="IVS267"
     primer_bind     6287..6305
                     /standard_name="IVS176"
     primer_bind     6303..6330
                     /standard_name="IVS269"
     misc_feature    complement(6402..6420)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="IVS177"
     misc_feature    complement(6443..6460)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="IVS268"
     primer_bind     6609..6630
                     /standard_name="Seq_0057_Ori"
     rep_origin      6780..7447
     primer_bind     6912..6931
                     /standard_name="Seq_0058_ori"
     primer_bind     7238..7257
                     /standard_name="IVS174"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgaggg cctatttccc atgattcctt
      181 catatttgca tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa
      241 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg
      301 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt
      361 tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccggagac gcgcttgtct
      421 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
      481 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accattaact
      541 ataacggtcc taaggtagcg aaatgcggtg tgaaataccg cacagatgcg taaggagaaa
      601 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
      661 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
      721 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattcga
      781 gctcgggcgt ctctgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
      841 caacttgaaa aagtggcacc gagtcggtgc tttttttcta gaggtacccg ttacataact
      901 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      961 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     1021 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     1081 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     1141 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt
     1201 gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta
     1261 tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg gggggcgcgc
     1321 gccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggagag gtgcggcggc
     1381 agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg
     1441 gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgacgctgc cttcgccccg
     1501 tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc
     1561 cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagctg agcaagaggt
     1621 aagggtttaa gggatggttg gttggtgggg tattaatgtt taattacctg gagcacctgc
     1681 ctgaaatcac tttttttcag gttggaccgg tgccaccatg cccaagaaga agaggaaggt
     1741 gtccaatctc ctgactgttc accagaacct ccctgcgctg ccagtagatg ccactagcga
     1801 tgaggtcagg aaaaatctca tggatatgtt tagggataga caggcgtttt ctgaacacac
     1861 ctggaaaatg ctgcttagcg tgtgccgatc ctgggcagcc tggtgtaagc tgaacaatcg
     1921 caaatggttc cccgccgagc cggaggacgt gcgcgattac ctgctgtatc tccaggcaag
     1981 agggctggct gtcaagacta tccagcagca cttgggccaa ctgaatatgc tgcatcgacg
     2041 cagcgggctc ccccggccta gcgattcaaa cgcagtctcc cttgttatga ggagaattag
     2101 aaaggaaaac gtagatgcgg gtgagagggc taagcaggct ctcgcttttg agcggactga
     2161 tttcgaccag gtcagatccc tgatggagaa cagcgatcgg tgccaggaca tcaggaacct
     2221 cgcatttctg ggaattgcat ataacacact tctgcgcata gctgagatcg cccggatcag
     2281 agtgaaagac atcagtcgaa cggacggcgg ccggatgctt attcatattg gacgcacaaa
     2341 gacattggtc agcaccgctg gcgttgaaaa ggccttgtcc ctgggcgtaa cgaagctggt
     2401 ggaaagatgg atctcagtgt ccggcgtggc tgacgaccct aataattact tgttctgtcg
     2461 agtgagaaaa aacggagtcg ccgcgccctc tgccaccagc caattgagta cacgggccct
     2521 tgaagggatc tttgaggcaa cccaccgact catatacgga gccaaggatg acagtggcca
     2581 gaggtatctc gcctggtcag gtcattctgc tagggtgggg gccgcacgag acatggcgcg
     2641 ggcaggagtc tccataccag agattatgca agctggaggt tggacaaatg tgaacatcgt
     2701 tatgaactat atccgcaatc ttgactctga aaccggggcc atggtgagac tgctcgaaga
     2761 tggtgactac ccatacgatg ttccagatta cgctgctagc ggcagtggag agggcagagg
     2821 aagtctgcta acatgcggtg acgtcgagga gaatcctggc ccaatggacg aaaagaccac
     2881 aggatggcga ggaggacacg tcgtcgaggg actggcagga gagctggaac agctgcgggc
     2941 tagactggaa caccatcctc agggacagcg agagccagga ggaggtggaa gcggaggagg
     3001 aggaagcgga ggaggaggta gcggatccgt gagcaagggc gaggagctgt tcaccggggt
     3061 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     3121 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     3181 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     3241 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     3301 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     3361 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     3421 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     3481 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     3541 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     3601 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     3661 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     3721 cggcatggac gagctgtaca agtccggact cagatctcga gaggaggagg aggagacaga
     3781 cagcaggatg ccccacctcg acagccccgg cagctcccag cctagacgct ccttcctctc
     3841 aagggtgatc agggcagcgc taccgttgca gctgcttctg ctgctgctgc tgctcctggc
     3901 ctgcctgcta cctgcctctg aagatgacta cagctgcacc caggccaaca actttgcccg
     3961 atccttctac cccatgctgc ggtacaccaa cgggccacct cccacctaag cctaggaatt
     4021 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     4081 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     4141 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     4201 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     4261 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     4321 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     4381 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     4441 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     4501 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     4561 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     4621 ctccccgcat cgataccgag cgctaataaa agatctttat tttcattaga tctgtgtgtt
     4681 ggttttttgt gtcacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc
     4741 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc
     4801 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc
     4861 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc
     4921 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     4981 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     5041 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     5101 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac
     5161 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     5221 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg ggctattctt
     5281 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     5341 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca
     5401 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     5461 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccttct
     5521 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg atacgaaagg
     5581 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     5641 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     5701 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     5761 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5821 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5881 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5941 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     6001 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     6061 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     6121 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     6181 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     6241 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     6301 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     6361 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     6421 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     6481 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     6541 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6601 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6661 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     6721 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6781 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6841 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6901 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     6961 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7021 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7081 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7141 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     7201 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     7261 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     7321 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     7381 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     7441 ttgctcacat gt
//
