LOCUS       pAS133_-_pAAV-EF1a-mTagBF2-KASH(BsmBIfree)-WPRE-SV40-BsmBIfreebb        6015 bp    DNA     circular UNA 19-OCT-2019
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...10-b3cwxc4
VERSION     urn.local...10-b3cwxc4
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /note="Geneious type: ITR"
                     /standard_name="L-ITR"
     misc_feature    155..179
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="TGAGGGCCTATTTCCCATGATTCCTTCATATTTGCATATACG
                     ATACAAGGCTGTTAGAGAGATAATTGGAATTAATTTGACTGTAAACACAAAGATATTA
                     GTACAAAATACGTGACGTAGAAAGTAATAATTTCTTGGGTAGTTTGCAGTTTTAAAAT
                     TATGTTTTAAAATGGACTATCATATGCTTACCGTAACTTGAAAGTATTTCGATTTCTT
                     GGCTTTATATATCTTGTGGAAAGGACGAAACACCGGAGACGCGCTTGTCTGTAAGCGG
                     ATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGG
                     CTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATTAACTATAA
                     CGGTCCTAAGGTAGCGAAATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAAT
                     ACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGT
                     GCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTA
                     AGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAAT
                     TCGAGCTCGGGCGTCTCTGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTC
                     CGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT"
     misc_feature    <180..>1461
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of
                     pCM198_pAAV-U6(SapI-SapI)Cbh-mCherryHA-WPRE-shpA -
                     Fragment 2 extraction (concatenated sequence 1)"
     misc_feature    <180..>1461
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pCM5_pAAV-EF1a-EYFP-W
                     (modified) - Fragment 2 (concatenated sequence 1)"
     misc_feature    <180..>1461
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pCM210 -
                     pAAV-U6(SapI-SapI)-CbH-mCherryHA-WPRE-shpA-BsmBIfree -
                     Fragment 2 - Digestion of
                     pCM198_pAAV-U6(SapI-SapI)Cbh-mCherryHA-WPRE-shpA -
                     Fragment 2 extraction (concatenated sequence 1)
                     (concatenated sequence 1)"
     misc_feature    185..2190
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="CTGCAGAGGGCCCTGCGTATGAGTGCAAGTGGGTTTTAGGAC
                     CAGGATGAGGCGGGGTGGGGGTGCCTACCTGACGACCGACCCCGACCCACTGGACAAG
                     CACCCAACCCCCATTCCCCAAATTGCGCATCCCCTATCAGAGAGGGGGAGGGGAAACA
                     GGATGCGGCGAGGCGCGTGCGCACTGCCAGCTTCAGCACCGCGGACAGTGCCTTCGCC
                     CCCGCCTGGCGGCGCGCGCCACCGCCGCCTCAGCACTGAAGGCGCGCTGACGTCACTC
                     GCCGGTCCCCCGCAAACTCCCCTTCCCGGCCACCTTGGTCGCGTCCGCGCCGCCGCCG
                     GCCCAGCCGGACCGCACCACGCGAGGCGCGAGATAGGGGGGCACGGGCGCGACCATCT
                     GCGCTGCGGCGCCGGCGACTCAGCGCTGCCTCAGTCTGCGGTGGGCAGCGGAGGAGTC
                     GTGTCGTGCCTGAGAGCGCAGTCGAGAAGGTACCGCCACCATGCCCAAGAAGAAGAGG
                     AAGGTGTCCAATCTCCTGACTGTTCACCAGAACCTCCCTGCGCTGCCAGTAGATGCCA
                     CTAGCGATGAGGTCAGGAAAAATCTCATGGATATGTTTAGGGATAGACAGGCGTTTTC
                     TGAACACACCTGGAAAATGCTGCTTAGCGTGTGCCGATCCTGGGCAGCCTGGTGTAAG
                     CTGAACAATCGCAAATGGTTCCCCGCCGAGCCGGAGGACGTGCGCGATTACCTGCTGT
                     ATCTCCAGGCAAGAGGGCTGGCTGTCAAGACTATCCAGCAGCACTTGGGCCAACTGAA
                     TATGCTGCATCGACGCAGCGGGCTCCCCCGGCCTAGCGATTCAAACGCAGTCTCCCTT
                     GTTATGAGGAGAATTAGAAAGGAAAACGTAGATGCGGGTGAGAGGGCTAAGCAGGCTC
                     TCGCTTTTGAGCGGACTGATTTCGACCAGGTCAGATCCCTGATGGAGAACAGCGATCG
                     GTGCCAGGACATCAGGAACCTCGCATTTCTGGGAATTGCATATAACACACTTCTGCGC
                     ATAGCTGAGATCGCCCGGATCAGAGTGAAAGACATCAGTCGAACGGACGGCGGCCGGA
                     TGCTTATTCATATTGGACGCACAAAGACATTGGTCAGCACCGCTGGCGTTGAAAAGGC
                     CTTGTCCCTGGGCGTAACGAAGCTGGTGGAAAGATGGATCTCAGTGTCCGGCGTGGCT
                     GACGACCCTAATAATTACTTGTTCTGTCGAGTGAGAAAAAACGGAGTCGCCGCGCCCT
                     CTGCCACCAGCCAATTGAGTACACGGGCCCTTGAAGGGATCTTTGAGGCAACCCACCG
                     ACTCATATACGGAGCCAAGGATGACAGTGGCCAGAGGTATCTCGCCTGGTCAGGTCAT
                     TCTGCTAGGGTGGGGGCCGCACGAGACATGGCGCGGGCAGGAGTCTCCATACCAGAGA
                     TTATGCAAGCTGGAGGTTGGACAAATGTGAACATCGTTATGAACTATATCCGCAATCT
                     TGACTCTGAAACCGGGGCCATGGTGAGACTGCTCGAAGATGGTGACTACCCATACGAT
                     GTTCCAGATTACGCTGCTAGCGGCAGTGGAGAGGGCAGAGGAAGTCTGCTAACATGCG
                     GTGACGTCGAGGAGAATCCTGGCCCAGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGT
                     GGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGCGC
                     GGCGAGGGCGAGGGCGATGCCACCAACGGCAAGCTGACCCTGAAGTTCATCTGCACCA
                     CCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCA
                     GTGCTTCAGCCGCTACCCCGACCACATGAAGCGCCACGACTTCTTCAAGTCCGCCATG
                     CCCGAAGGCTACGTCCAGGAGCGCACCATCAGCTTCAAGGACGACGGCACCTACAAGA
                     CCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGG
                     CATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTTCAAC
                     AGCCACAACGTCTATATCACCGCCGACAAGCAGAAGAACGGCATCAAGGCCAACTTCA
                     AGATCCGCCACAACGTGGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAA
                     CACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAG
                     TCCGTGCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCG
                     TGACCGCCGCCGGGATCACTCACGGCATGGACGAGCTGTACAAG"
     misc_feature    198..>1461
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pCM155 - PBdonor -
                     IR-IRES-2A-EGFP-EF1alpha-tdTomato-IR (modified) (modified)
                     - Fragment 2 (concatenated sequence 1)"
     misc_feature    <198..1461
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pCM171 - PBdonor -
                     U6_Feng_AarI(2x)_F+E_Scaffold-EF1alpha-tdTomato-IR -
                     Fragment 2 extraction extraction (concatenated sequence
                     1)"
     misc_feature    201..232
                     /note="Geneious type: PCR_primer"
                     /standard_name="PCR primer"
     promoter        201..1459
     primer_bind     201..232
                     /standard_name="FS_01_Ef1alpha_PacI_fw"
     misc_feature    <201..232
                     /note
                     /standard_name="misc"
     misc_feature    589
                     /note="insertion"
                     /standard_name="insertion"
     misc_feature    complement(1431..1459)
                     /note="Geneious type: PCR_primer"
                     /standard_name="PCR primer"
     misc_feature    complement(1431..1459)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="FS_04_Ef1alpha_BpiI_AgeI_rv"
     misc_feature    complement(1431..>1459)
                     /note
                     /standard_name="misc"
     misc_feature    1457..>1459
                     /note
                     /standard_name="misc"
     misc_feature    complement(1460..>1461)
                     /standard_name="Enzyme_overlap"
     misc_feature    1462..1465
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    complement(<1462..1465)
                     /standard_name="Enzyme_overlap"
     source          1480..2190
                     /organism="mTagBFP2-H3.3-N-14"
                     /mol_type="other DNA"
     CDS             1480..2190
                     /translation="MARTKQTARKSTGGKAPRKQLATKAARKSAPSTGGVKKPHRYRP
                     GTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAYLV
                     GLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERAGSGSAGSGDPPVATMVSKGEELIK
                     ENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVVEGGPLPFAFDILATSFLY
                     GSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLIYNVKIR
                     GVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLVGGSHLIANAKTTYRS
                     KKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCDLPSKLGHKLN*"
                     /standard_name="ORF frame 1"
     CDS             1480..2190
                     /standard_name="mTagBFP2"
     CDS             complement(1480..1998)
                     /translation="MAPAHELQGHVVSAFQAAVSGVQRLGEGLPAECFLLHHRAVGCE
                     VHPSDLDVVDEAAVLEAGVLGSGQHAPVFVCGDSLPCEALREGLLEEVGDALGVVDEG
                     LAAVEEASSQDVEGEGERAALDHLDSHGLGALVGLAFALGCALEVMVVHGALHVQLHV
                     HVLLNQLFALRHHGGDRWIPTACASGPSSLPPYPASQLDVFGHDGDSLGVDGTQIGIF
                     KQPHQVCFASLLKGTDGCTLKPQVGFEILGDLPHQSLEWQLPDEQLSRFLVTTDLSQS
                     HGPWPVAVRLLHPAGRGRAFPSRLGGQLFAGGFPTGGLPSGLLGSGHGGEA*"
                     /standard_name="ORF frame 2"
     primer_bind     1480..1501
                     /standard_name="IVS228_mTagBFP2_AscI_fw"
     misc_feature    1480..2190
                     /standard_name="Digestion of mTagBFP2-H3.3-N-14 (modified)
                     PCR"
     primer_bind     2165..2207
                     /standard_name="IVS327_KASH_Gibson_ovl_mTagBFP2_fw"
     misc_feature    complement(2168..2190)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="IVS179"
     misc_feature    2191..2208
                     /note="misc"
                     /standard_name="misc"
     CDS             2209..>2306
                     /standard_name="KASH domain of Nesprin2"
     misc_feature    2271
                     /note="silent mutation to remove BsmBI site"
                     /standard_name="misc"
     CDS             <2307..2454
                     /codon_start=2
                     /standard_name="KASH domain of Nesprin2"
     misc_feature    complement(2441..2458)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="EG023"
     misc_feature    2478..>2483
                     /note="WPRE"
                     /note="Geneious type: 3'UTR"
                     /standard_name="WPRE"
     misc_feature    <2484..3073
                     /note="WPRE"
                     /note="Geneious type: 3'UTR"
                     /standard_name="WPRE"
     misc_feature    complement(2541..2561)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="Seq_0017_WPRE_rev"
     primer_bind     2774..2791
                     /standard_name="IVS395_ddPCR_WPRE_fw"
     misc_feature    2793..2809
                     /note="Geneious type: DNA probe bind"
                     /standard_name="IVS397_ddPCR_WPRE_Probe"
     misc_feature    complement(2857..2874)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="IVS396_ddPCR_WPRE_rv"
     misc_feature    3079..3103
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="TGAGGGCCTATTTCCCATGATTCCTTCATATTTGCATATACG
                     ATACAAGGCTGTTAGAGAGATAATTGGAATTAATTTGACTGTAAACACAAAGATATTA
                     GTACAAAATACGTGACGTAGAAAGTAATAATTTCTTGGGTAGTTTGCAGTTTTAAAAT
                     TATGTTTTAAAATGGACTATCATATGCTTACCGTAACTTGAAAGTATTTCGATTTCTT
                     GGCTTTATATATCTTGTGGAAAGGACGAAACACCGGAGACGCGCTTGTCTGTAAGCGG
                     ATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGG
                     CTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATTAACTATAA
                     CGGTCCTAAGGTAGCGAAATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAAT
                     ACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGT
                     GCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTA
                     AGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAAT
                     TCGAGCTCGGGCGTCTCTGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTC
                     CGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT"
     polyA_signal    3120..>3253
     primer_bind     3120..3145
                     /standard_name="AS105"
     misc_feature    3121..3254
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="CCGAGCGCTAATAAAAGATCTTTATTTTCATTAGATCTGTGT
                     GTTGGTTTTTTGTG"
     primer_bind     complement(3234..3253)
                     /standard_name="AS106"
     misc_feature    3278..3418
                     /note="Geneious type: ITR"
                     /standard_name="R-ITR"
     misc_feature    complement(3428..3448)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="Seq_0060"
     rep_origin      3496..3923
     misc_feature    3709
                     /standard_name="A->T F1 ori mutation"
     primer_bind     3790..3810
                     /standard_name="Seq_0025"
     misc_feature    3835
                     /standard_name="T->G F1 ori mutation"
     misc_feature    3922
                     /standard_name="A->T F1 ori mutation"
     misc_feature    4080
                     /standard_name="G->T rem.BsmBI"
     misc_feature    4135
                     /standard_name="G->T rem.BsmBI"
     misc_feature    4265..4293
                     /note="Geneious type: Bacterial Promoter"
                     /standard_name="Ampicillin promoter"
     misc_feature    4335..4403
                     /note="Geneious type: Bacterial CDS"
                     /standard_name="BLa Signalpeptide"
     misc_feature    4404..5192
                     /note="Geneious type: Bacterial CDS"
                     /standard_name="AmpR"
     primer_bind     5172..5193
                     /standard_name="Seq_0057_Ori"
     rep_origin      5343..6010
     primer_bind     5475..5494
                     /standard_name="Seq_0058_ori"
     misc_feature    5513
                     /standard_name="T->C pUC ori mutation"
     misc_feature    5983
                     /standard_name="C->T pUC ori mutation"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgggaggg cctattctgc agacaaatgc
      181 tagaggtacc cgttaattaa tgcaaagatg gataaagttt taaacagaga ggaatctttg
      241 cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg
      301 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
      361 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
      421 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
      481 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
      541 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
      601 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
      661 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
      721 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
      781 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
      841 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
      901 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
      961 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     1021 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     1081 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     1141 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     1201 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     1261 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     1321 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     1381 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     1441 tccatttcag gtgtcgtgag gcgcgccacc ggtgccacca tggtgtctaa gggcgaagag
     1501 ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgga caaccatcac
     1561 ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc
     1621 aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac tagcttcctc
     1681 tacggcagca agaccttcat caaccacacc cagggcatcc ccgacttctt caagcagtcc
     1741 ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc
     1801 gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg
     1861 gtgaacttca catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccttc
     1921 accgagacgc tgtaccccgc tgacggcggc ctggaaggca gaaacgacat ggccctgaag
     1981 ctcgtgggcg ggagccatct gatcgcaaac gccaagacca catatagatc caagaaaccc
     2041 gctaagaacc tcaagatgcc tggcgtctac tatgtggact acagactgga aagaatcaag
     2101 gaggccaaca acgagaccta cgtcgagcag cacgaggtgg cagtggccag atactgcgac
     2161 ctccctagca aactggggca caagcttaat tccggactca gatctcgaga ggaggaggag
     2221 gagacagaca gcaggatgcc ccacctcgac agccccggca gctcccagcc tagacgctcc
     2281 ttcctctcaa gggtgatcag ggcagcgcta ccgttgcagc tgcttctgct gctgctgctg
     2341 ctcctggcct gcctgctacc tgcctctgaa gatgactaca gctgcaccca ggccaacaac
     2401 tttgcccgat ccttctaccc catgctgcgg tacaccaacg ggccacctcc cacctaagcc
     2461 taggaattcg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
     2521 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     2581 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     2641 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     2701 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     2761 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     2821 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     2881 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     2941 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     3001 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     3061 tgggccgcct cccacgcggg agggcctatt ctgcagacaa atgctagaat gcaatcgata
     3121 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     3181 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     3241 atcatgtctg gatctcacgt gcggaccgag cggccgcagg aacccctagt gatggagttg
     3301 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
     3361 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg
     3421 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
     3481 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     3541 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     3601 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     3661 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
     3721 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     3781 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt
     3841 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     3901 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
     3961 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     4021 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgacct
     4081 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgatacgaa
     4141 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     4201 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4261 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4321 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     4381 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     4441 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     4501 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     4561 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     4621 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     4681 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     4741 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     4801 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     4861 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     4921 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     4981 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     5041 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     5101 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     5161 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     5221 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     5281 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     5341 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     5401 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     5461 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     5521 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     5581 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     5641 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     5701 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     5761 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     5821 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     5881 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     5941 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     6001 cttttgctca catgt
//
