LOCUS       pAS134_-_pAAV-EF1a-Venus-KASH(BsmBIfree)-WPRE-SV40-BsmBIfreebb        6012 bp    DNA     circular UNA 19-OCT-2019
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...47-b3cy2vw
VERSION     urn.local...47-b3cy2vw
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /note="Geneious type: ITR"
                     /standard_name="L-ITR"
     misc_feature    155..179
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="TGAGGGCCTATTTCCCATGATTCCTTCATATTTGCATATACG
                     ATACAAGGCTGTTAGAGAGATAATTGGAATTAATTTGACTGTAAACACAAAGATATTA
                     GTACAAAATACGTGACGTAGAAAGTAATAATTTCTTGGGTAGTTTGCAGTTTTAAAAT
                     TATGTTTTAAAATGGACTATCATATGCTTACCGTAACTTGAAAGTATTTCGATTTCTT
                     GGCTTTATATATCTTGTGGAAAGGACGAAACACCGGAGACGCGCTTGTCTGTAAGCGG
                     ATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGG
                     CTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATTAACTATAA
                     CGGTCCTAAGGTAGCGAAATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAAT
                     ACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGT
                     GCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTA
                     AGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAAT
                     TCGAGCTCGGGCGTCTCTGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTC
                     CGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT"
     misc_feature    <180..>1461
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of
                     pCM198_pAAV-U6(SapI-SapI)Cbh-mCherryHA-WPRE-shpA -
                     Fragment 2 extraction (concatenated sequence 1)"
     misc_feature    <180..>1461
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pCM5_pAAV-EF1a-EYFP-W
                     (modified) - Fragment 2 (concatenated sequence 1)"
     misc_feature    <180..>1461
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pCM210 -
                     pAAV-U6(SapI-SapI)-CbH-mCherryHA-WPRE-shpA-BsmBIfree -
                     Fragment 2 - Digestion of
                     pCM198_pAAV-U6(SapI-SapI)Cbh-mCherryHA-WPRE-shpA -
                     Fragment 2 extraction (concatenated sequence 1)
                     (concatenated sequence 1)"
     misc_feature    185..2179
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="CTGCAGAGGGCCCTGCGTATGAGTGCAAGTGGGTTTTAGGAC
                     CAGGATGAGGCGGGGTGGGGGTGCCTACCTGACGACCGACCCCGACCCACTGGACAAG
                     CACCCAACCCCCATTCCCCAAATTGCGCATCCCCTATCAGAGAGGGGGAGGGGAAACA
                     GGATGCGGCGAGGCGCGTGCGCACTGCCAGCTTCAGCACCGCGGACAGTGCCTTCGCC
                     CCCGCCTGGCGGCGCGCGCCACCGCCGCCTCAGCACTGAAGGCGCGCTGACGTCACTC
                     GCCGGTCCCCCGCAAACTCCCCTTCCCGGCCACCTTGGTCGCGTCCGCGCCGCCGCCG
                     GCCCAGCCGGACCGCACCACGCGAGGCGCGAGATAGGGGGGCACGGGCGCGACCATCT
                     GCGCTGCGGCGCCGGCGACTCAGCGCTGCCTCAGTCTGCGGTGGGCAGCGGAGGAGTC
                     GTGTCGTGCCTGAGAGCGCAGTCGAGAAGGTACCGCCACCATGCCCAAGAAGAAGAGG
                     AAGGTGTCCAATCTCCTGACTGTTCACCAGAACCTCCCTGCGCTGCCAGTAGATGCCA
                     CTAGCGATGAGGTCAGGAAAAATCTCATGGATATGTTTAGGGATAGACAGGCGTTTTC
                     TGAACACACCTGGAAAATGCTGCTTAGCGTGTGCCGATCCTGGGCAGCCTGGTGTAAG
                     CTGAACAATCGCAAATGGTTCCCCGCCGAGCCGGAGGACGTGCGCGATTACCTGCTGT
                     ATCTCCAGGCAAGAGGGCTGGCTGTCAAGACTATCCAGCAGCACTTGGGCCAACTGAA
                     TATGCTGCATCGACGCAGCGGGCTCCCCCGGCCTAGCGATTCAAACGCAGTCTCCCTT
                     GTTATGAGGAGAATTAGAAAGGAAAACGTAGATGCGGGTGAGAGGGCTAAGCAGGCTC
                     TCGCTTTTGAGCGGACTGATTTCGACCAGGTCAGATCCCTGATGGAGAACAGCGATCG
                     GTGCCAGGACATCAGGAACCTCGCATTTCTGGGAATTGCATATAACACACTTCTGCGC
                     ATAGCTGAGATCGCCCGGATCAGAGTGAAAGACATCAGTCGAACGGACGGCGGCCGGA
                     TGCTTATTCATATTGGACGCACAAAGACATTGGTCAGCACCGCTGGCGTTGAAAAGGC
                     CTTGTCCCTGGGCGTAACGAAGCTGGTGGAAAGATGGATCTCAGTGTCCGGCGTGGCT
                     GACGACCCTAATAATTACTTGTTCTGTCGAGTGAGAAAAAACGGAGTCGCCGCGCCCT
                     CTGCCACCAGCCAATTGAGTACACGGGCCCTTGAAGGGATCTTTGAGGCAACCCACCG
                     ACTCATATACGGAGCCAAGGATGACAGTGGCCAGAGGTATCTCGCCTGGTCAGGTCAT
                     TCTGCTAGGGTGGGGGCCGCACGAGACATGGCGCGGGCAGGAGTCTCCATACCAGAGA
                     TTATGCAAGCTGGAGGTTGGACAAATGTGAACATCGTTATGAACTATATCCGCAATCT
                     TGACTCTGAAACCGGGGCCATGGTGAGACTGCTCGAAGATGGTGACTACCCATACGAT
                     GTTCCAGATTACGCTGCTAGCGGCAGTGGAGAGGGCAGAGGAAGTCTGCTAACATGCG
                     GTGACGTCGAGGAGAATCCTGGCCCAGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGT
                     GGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGCGC
                     GGCGAGGGCGAGGGCGATGCCACCAACGGCAAGCTGACCCTGAAGTTCATCTGCACCA
                     CCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCA
                     GTGCTTCAGCCGCTACCCCGACCACATGAAGCGCCACGACTTCTTCAAGTCCGCCATG
                     CCCGAAGGCTACGTCCAGGAGCGCACCATCAGCTTCAAGGACGACGGCACCTACAAGA
                     CCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGG
                     CATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTTCAAC
                     AGCCACAACGTCTATATCACCGCCGACAAGCAGAAGAACGGCATCAAGGCCAACTTCA
                     AGATCCGCCACAACGTGGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAA
                     CACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAG
                     TCCGTGCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCG
                     TGACCGCCGCCGGGATCACTCACGGCATGGACGAGC"
     misc_feature    198..>1461
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pCM155 - PBdonor -
                     IR-IRES-2A-EGFP-EF1alpha-tdTomato-IR (modified) (modified)
                     - Fragment 2 (concatenated sequence 1)"
     misc_feature    <198..1461
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pCM171 - PBdonor -
                     U6_Feng_AarI(2x)_F+E_Scaffold-EF1alpha-tdTomato-IR -
                     Fragment 2 extraction extraction (concatenated sequence
                     1)"
     misc_feature    201..232
                     /note="Geneious type: PCR_primer"
                     /standard_name="PCR primer"
     promoter        201..1459
     primer_bind     201..232
                     /standard_name="FS_01_Ef1alpha_PacI_fw"
     misc_feature    <201..232
                     /note
                     /standard_name="misc"
     misc_feature    589
                     /note="insertion"
                     /standard_name="insertion"
     misc_feature    complement(1431..1459)
                     /note="Geneious type: PCR_primer"
                     /standard_name="PCR primer"
     misc_feature    complement(1431..1459)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="FS_04_Ef1alpha_BpiI_AgeI_rv"
     misc_feature    complement(1431..>1459)
                     /note
                     /standard_name="misc"
     misc_feature    1457..>1459
                     /note
                     /standard_name="misc"
     misc_feature    complement(1460..>1461)
                     /standard_name="Enzyme_overlap"
     misc_feature    1462..1465
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    complement(<1462..>1465)
                     /standard_name="Enzyme_overlap"
     misc_feature    <1468..>2187
                     /note="Geneious type: CDS"
                     /standard_name="Venus"
     primer_bind     1468..1483
                     /standard_name="FS_2480_Venus_AscI-Kozak-ATG-fw"
     primer_bind     1468..1483
                     /standard_name="FS_2525_AflII_(GSG)-P2A_Venus_fw"
     misc_feature    complement(2166..2187)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="FS_2481_Venus_Kpn2I_rv"
     source          <2189..>2303
     misc_feature    2189..2192
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    <2189..2205
                     /note
                     /standard_name="misc"
     CDS             2206..>2303
                     /standard_name="KASH domain of Nesprin2"
     misc_feature    2268
                     /note="silent mutation to remove BsmBI site"
                     /standard_name="misc"
     CDS             <2304..2451
                     /codon_start=2
                     /standard_name="KASH domain of Nesprin2"
     misc_feature    complement(2438..2455)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="EG023"
     misc_feature    2475..>2480
                     /note="WPRE"
                     /note="Geneious type: 3'UTR"
                     /standard_name="WPRE"
     misc_feature    <2481..3070
                     /note="WPRE"
                     /note="Geneious type: 3'UTR"
                     /standard_name="WPRE"
     misc_feature    complement(2538..2558)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="Seq_0017_WPRE_rev"
     primer_bind     2771..2788
                     /standard_name="IVS395_ddPCR_WPRE_fw"
     misc_feature    2790..2806
                     /note="Geneious type: DNA probe bind"
                     /standard_name="IVS397_ddPCR_WPRE_Probe"
     misc_feature    complement(2854..2871)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="IVS396_ddPCR_WPRE_rv"
     misc_feature    3076..3100
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="TGAGGGCCTATTTCCCATGATTCCTTCATATTTGCATATACG
                     ATACAAGGCTGTTAGAGAGATAATTGGAATTAATTTGACTGTAAACACAAAGATATTA
                     GTACAAAATACGTGACGTAGAAAGTAATAATTTCTTGGGTAGTTTGCAGTTTTAAAAT
                     TATGTTTTAAAATGGACTATCATATGCTTACCGTAACTTGAAAGTATTTCGATTTCTT
                     GGCTTTATATATCTTGTGGAAAGGACGAAACACCGGAGACGCGCTTGTCTGTAAGCGG
                     ATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGG
                     CTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATTAACTATAA
                     CGGTCCTAAGGTAGCGAAATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAAT
                     ACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGT
                     GCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTA
                     AGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAAT
                     TCGAGCTCGGGCGTCTCTGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTC
                     CGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT"
     polyA_signal    3117..>3250
     primer_bind     3117..3142
                     /standard_name="AS105"
     misc_feature    3118..3251
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="CCGAGCGCTAATAAAAGATCTTTATTTTCATTAGATCTGTGT
                     GTTGGTTTTTTGTG"
     primer_bind     complement(3231..3250)
                     /standard_name="AS106"
     misc_feature    3275..3415
                     /note="Geneious type: ITR"
                     /standard_name="R-ITR"
     misc_feature    complement(3425..3445)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="Seq_0060"
     rep_origin      3493..3920
     misc_feature    3706
                     /standard_name="A->T F1 ori mutation"
     primer_bind     3787..3807
                     /standard_name="Seq_0025"
     misc_feature    3832
                     /standard_name="T->G F1 ori mutation"
     misc_feature    3919
                     /standard_name="A->T F1 ori mutation"
     misc_feature    4077
                     /standard_name="G->T rem.BsmBI"
     misc_feature    4132
                     /standard_name="G->T rem.BsmBI"
     misc_feature    4262..4290
                     /note="Geneious type: Bacterial Promoter"
                     /standard_name="Ampicillin promoter"
     misc_feature    4332..4400
                     /note="Geneious type: Bacterial CDS"
                     /standard_name="BLa Signalpeptide"
     misc_feature    4401..5189
                     /note="Geneious type: Bacterial CDS"
                     /standard_name="AmpR"
     primer_bind     5169..5190
                     /standard_name="Seq_0057_Ori"
     rep_origin      5340..6007
     primer_bind     5472..5491
                     /standard_name="Seq_0058_ori"
     misc_feature    5510
                     /standard_name="T->C pUC ori mutation"
     misc_feature    5980
                     /standard_name="C->T pUC ori mutation"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgggaggg cctattctgc agacaaatgc
      181 tagaggtacc cgttaattaa tgcaaagatg gataaagttt taaacagaga ggaatctttg
      241 cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg
      301 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
      361 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
      421 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
      481 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
      541 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
      601 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
      661 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
      721 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
      781 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
      841 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
      901 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
      961 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     1021 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     1081 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     1141 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     1201 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     1261 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     1321 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     1381 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     1441 tccatttcag gtgtcgtgag gcgcgccacc atggtcagca agggagaaga acttttcacg
     1501 ggcgtggtac ctatcctcgt ggagctggac ggtgatgtga acggacataa gtttagcgtt
     1561 tctggtgaag gcgaagggga cgctacgtac ggtaagctca cgctcaaact gatctgtacc
     1621 accgggaagc tgcccgtgcc ttggccgaca ttggttacta ccctggggta tggcctccag
     1681 tgtttcgcaa gataccccga ccacatgaag cagcacgact tctttaaaag cgccatgcca
     1741 gaaggatacg tccaggagag gacgatattt tttaaggatg atggcaatta taagacccgc
     1801 gcagaagtta aattcgaagg ggatactctg gtgaacagga tcgagttgaa ggggatagat
     1861 tttaaggagg acggtaatat tctcgggcac aaattggagt acaactataa cagtcacaat
     1921 gtttatatca ccgccgataa gcaaaagaac ggcataaaag ccaattttaa gatcagacat
     1981 aatatcgagg acggcggggt ccagctcgcc gaccattatc agcagaatac cccaataggc
     2041 gatggaccag tactcttgcc tgacaaccat tacctgtctt accaaagcgc actgtcaaag
     2101 gacccaaacg agaaacggga tcatatggtt ctcctggaat tcgtcacagc agccgggatc
     2161 acactcggga tggacgagtt gtacaagtcc ggactcagat ctcgagagga ggaggaggag
     2221 acagacagca ggatgcccca cctcgacagc cccggcagct cccagcctag acgctccttc
     2281 ctctcaaggg tgatcagggc agcgctaccg ttgcagctgc ttctgctgct gctgctgctc
     2341 ctggcctgcc tgctacctgc ctctgaagat gactacagct gcacccaggc caacaacttt
     2401 gcccgatcct tctaccccat gctgcggtac accaacgggc cacctcccac ctaagcctag
     2461 gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     2521 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     2581 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     2641 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     2701 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     2761 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     2821 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     2881 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     2941 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     3001 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     3061 gccgcctccc acgcgggagg gcctattctg cagacaaatg ctagaatgca atcgataact
     3121 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     3181 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     3241 atgtctggat ctcacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc
     3301 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc
     3361 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc
     3421 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc
     3481 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     3541 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     3601 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     3661 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac
     3721 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     3781 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg ggctattctt
     3841 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     3901 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca
     3961 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     4021 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccttct
     4081 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg atacgaaagg
     4141 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     4201 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4261 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4321 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     4381 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     4441 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     4501 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     4561 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     4621 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     4681 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     4741 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     4801 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     4861 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     4921 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     4981 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     5041 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     5101 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     5161 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     5221 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5281 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5341 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5401 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5461 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     5521 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     5581 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     5641 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     5701 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     5761 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     5821 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5881 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5941 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6001 ttgctcacat gt
//
