LOCUS       pPB_CMV_PARP1_GF       10141 bp    DNA        circular     06-JUN-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     complement(7306..7325)
                     /locus_tag="T3"
                     /label="T3"
                     /ApEinfo_label="T3"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6857..7166
                     /locus_tag="PB3"
                     /label="PB3"
                     /ApEinfo_label="PB3"
                     /ApEinfo_fwdcolor="#0cfeff"
                     /ApEinfo_revcolor="#0cfeff"
                     /ApEinfo_graphicformat="arrow_data {{} {0 1 2 0 0 -1} 0}
                     width 5 offset 0"
     promoter        739..971
                     /locus_tag="CMV"
                     /label="CMV"
                     /ApEinfo_label="CMV"
                     /ApEinfo_fwdcolor="#13ff92"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     10095..10112
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(7343..7363)
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    58..320
                     /locus_tag="PB5"
                     /label="PB5"
                     /ApEinfo_label="PB5"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     10121..10141
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        5562..5599
                     /locus_tag="gb3"
                     /label="gb3"
                     /ApEinfo_label="gb3"
                     /ApEinfo_fwdcolor="#1014ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(7731..8413)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6788..6810
                     /locus_tag="primer-right"
                     /label="primer-right"
                     /ApEinfo_label="primer-right"
                     /ApEinfo_fwdcolor="#ff0006"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(9631..9937)
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(9956..10024)
                     /locus_tag="LacZ alpha"
                     /label="LacZ alpha"
                     /ApEinfo_label="LacZ alpha"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    416..437
                     /locus_tag="primer-Left"
                     /label="primer-Left"
                     /ApEinfo_label="primer-Left"
                     /ApEinfo_fwdcolor="#ff0612"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{} {0 1 2 0 0 -1} 0}
                     width 5 offset 0"
     misc_binding    complement(7369..7391)
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        4969..5548
                     /locus_tag="ires"
                     /label="ires"
                     /ApEinfo_label="ires"
                     /ApEinfo_fwdcolor="#ff970a"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(509..538)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /locus_tag="lacZ_a"
                     /label="lacZ_a"
                     /ApEinfo_label="lacZ_a"
                     /ApEinfo_fwdcolor="#ff4000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(8511..9170)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    509..531
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /locus_tag="M13_pUC_fwd_primer"
                     /label="M13_pUC_fwd_primer"
                     /ApEinfo_label="M13_pUC_fwd_primer"
                     /ApEinfo_fwdcolor="#ff4000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            4947..4948
                     /gene="ECFP (468 - 713)"
                     /locus_tag="ECFP (468 - 713)"
                     /label="ECFP (468 - 713)"
                     /ApEinfo_label="ECFP (468 - 713)"
                     /ApEinfo_fwdcolor="#01ff0b"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    438..458
                     /locus_tag="p5+mir3"
                     /label="p5+mir3"
                     /ApEinfo_label="p5+mir3"
                     /ApEinfo_fwdcolor="#32ff82"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        524..540
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /locus_tag="M13_forward20_primer"
                     /label="M13_forward20_primer"
                     /ApEinfo_label="M13_forward20_primer"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             5600..6446
                     /locus_tag="neo"
                     /label="neo"
                     /ApEinfo_label="neo"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    571..738
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /locus_tag="CAG_enhancer"
                     /label="CAG_enhancer"
                     /ApEinfo_label="CAG_enhancer"
                     /ApEinfo_fwdcolor="#ff4000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    484..502
                     /locus_tag="p7+Loop"
                     /label="p7+Loop"
                     /ApEinfo_label="p7+Loop"
                     /ApEinfo_fwdcolor="#07ffa6"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{} {0 1 2 0 0 -1} 0}
                     width 5 offset 0"
     promoter        576..738
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /locus_tag="CMV_immearly_promoter"
                     /label="CMV_immearly_promoter"
                     /ApEinfo_label="CMV_immearly_promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            1154..4195
                     /gene="PARP1"
                     /gene_synonym="ADPRT; ADPRT 1; ADPRT1; ARTD1; pADPRT-1;
                     PARP; PARP-1; PPOL"
                     /note="poly (ADP-ribose) polymerase 1"
                     /db_xref="GeneID:142"
                     /db_xref="HGNC:270"
                     /db_xref="MIM:173870"
                     /locus_tag="PARP1"
                     /label="PARP1"
                     /ApEinfo_label="PARP1"
                     /ApEinfo_fwdcolor="#ffe511"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_site      6569..6690
                     /locus_tag="SV40 polyA"
                     /label="SV40 polyA"
                     /ApEinfo_label="SV40 polyA"
                     /ApEinfo_fwdcolor="#0fff34"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1112..1135
                     /translation="MDYKDDDDKLAAANSSIDLISVPVDSRRSIATMVSKGEELFTGV
                     VPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQ
                     CFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKG
                     IDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIE*"
                     /locus_tag="FLAG"
                     /label="FLAG"
                     /ApEinfo_label="FLAG"
                     /ApEinfo_fwdcolor="#ff4000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{} {0 1 2 0 0 -1} 0}
                     width 5 offset 0"
     misc_feature    6720..6741
                     /locus_tag="PB3seqF"
                     /label="PB3seqF"
                     /ApEinfo_label="PB3seqF"
                     /ApEinfo_fwdcolor="#00ff4d"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6767..6787
                     /locus_tag="PB3seq_R"
                     /label="PB3seq_R"
                     /ApEinfo_label="PB3seq_R"
                     /ApEinfo_fwdcolor="#11ff66"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{} {0 1 2 0 0 -1} 0}
                     width 5 offset 0"
     primer_bind     6887..6914
                     /locus_tag="PB3_seq_R"
                     /label="PB3_seq_R"
                     /ApEinfo_label="PB3_seq_R"
                     /ApEinfo_fwdcolor="#ffe609"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{} {0 1 2 0 0 -1} 0}
                     width 5 offset 0"
     misc_feature    459..483
                     /locus_tag="PB5 barcode"
                     /label="PB5 barcode"
                     /ApEinfo_label="PB5 barcode"
                     /ApEinfo_fwdcolor="#f111ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{} {} 0} width 5
                     offset 0"
     misc_feature    6742..6766
                     /locus_tag="PB3 barcode"
                     /label="PB3 barcode"
                     /ApEinfo_label="PB3 barcode"
                     /ApEinfo_fwdcolor="#dc1dff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{} {} 0} width 5
                     offset 0"
     CDS             5655..6446
                     /locus_tag="Kan/neoR"
                     /label="Kan/neoR"
                     /ApEinfo_label="Kan/neoR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             4232..4946
                     /locus_tag="EGFP"
                     /label="EGFP"
                     /ApEinfo_label="EGFP"
                     /ApEinfo_fwdcolor="#75ff8f"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 CGAATTGGCG GTATTCACGA CAGCAGGCTG AATAATAAAA AAATTAGAAA CTATTATTTA
       61 ACCCTAGAAA GATAATCATA TTGTGACGTA CGTTAAAGAT AATCATGCGT AAAATTGACG
      121 CATGTGTttt atcggtctgt atatcgaggt ttatttaTTA ATTTGAATAG ATATTAAGTT
      181 TTATTATATT TACACTTACA TACTAATAAT AAATTCAACA AACAATTTAT TTATGTTTAT
      241 TTATTTATTA AAAAAAAACA AAAACTCAAA ATTTCTTCTA TAAAGTAACA AAACTTTTAA
      301 ACATTCTCTC TTTTACAAAA ataaacttat tttgtacttt aaaaacagtc atgttgtatt
      361 ataaaataag taattagctt aacctataca taatagaaac aaattatact tattaGTCAG
      421 TCAGAAACAA CTTTGGCCTA AAGTAGCCCC TTGAATTCNN NNNNNNNNNN NNNNNNNNNN
      481 NNNTACATCT GTggcttcac tagctagcCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT
      541 GAGTTCGAGC TTGCATGCCT GCAGGTCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC
      601 TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG
      661 CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG
      721 GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA
      781 TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC
      841 ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG
      901 CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG
      961 AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA
     1021 TTGAcgcaaa tgggcggtag gcgtgTACGG TGGGAGGTCT ATATAAGCAG AGCTGGTTTA
     1081 GTGAACCGTC AGATCCgcta gcgccaccAT GGACTACAAA GACGATGACG ACAAGCTTGC
     1141 cGCCGCGAAT TCaatggcgg agTCTTCGGA TAAGCTCtat CGAGTCGAGT ACGCCAAGAG
     1201 cgggCGCgcc tcttgcaaga aaTGCAGCga gagcatcccc aaggactcgc tccggatggc
     1261 catcatggtg cagtcgccca tgTTTgatgg aaaagtccca cactggtacc acttctcctg
     1321 cttctggaag gtgggccact ccatccggca ccctgacgtt gaggtggatg ggttctctga
     1381 gcttcggtgg gatgaccagc agaaagtcaa gaagacagcg GAAgctggag gagtgacaGG
     1441 CAAAGgccag gatggaattg gtagcaaggc agagaagact ctgggtgact ttgcagcaga
     1501 gtatgCCAAG TCCAACAGAA GTACGTGCaa ggggtgtatg gagaagatag aaaagggcca
     1561 ggtgcgcctg tccaagaaga tggtggaccc ggagaagcca cagctaggca tgattgaccg
     1621 ctggtaccat ccaggctgct ttgtcaagaa cagggaggag ctgggtttcc ggcccgagta
     1681 cagtgcgagt cagctcaagg gcttcagcct ccttgctaca gaggataaag aagccctgaa
     1741 gaagcagctc ccaggagtca agagtgaagg aaagagaaaa ggcgatgagg tggatggagt
     1801 ggatgaagtg gcgaagaaga aatctaaaaa agaaaaagac aaggatagta agcttgaaaa
     1861 agccctaaag gctcagaacg acctgatctg gaacatcaag gacgagctaa agaaagtgtg
     1921 ttcaactaat gacctgaagg agctactcat cttcaacaag cagcaagtgc ctTCTgggGA
     1981 Gtcggcgatc ttggaccgag tagctgatgg catggtgttc ggtgccctcc ttccctgcga
     2041 ggaatgctcg ggtcagctgg tcttcaagag cgatgcctat tactgcactg gggacgtcac
     2101 tgcctggacc aagtgtATGg tcaagacaca gacacCCAAC CGGAAGGAGT GGGTAACCcc
     2161 aaaggaattc cgagaaatct cttacctcaa gaaattgaag gttaaaaagc aggaccgtat
     2221 attcccccca gaaaccagcg cctccgtggc ggccacgcct ccgccctcca cagcctcggc
     2281 tcctgctgct gtgaactcct ctgcttcagc agataagcca ttatccaaca tgaagatcct
     2341 gactctcggg aagctgtccc ggaacaagga tgaagtgaag gccatgattg agaaactcgg
     2401 ggggaagttg acggggacgg ccaacaaggc ttccctgtgc atcagcacca aaaaggaggt
     2461 ggaaaagatg aataagaaga tggaggaagt aaaggaagcc aacatccgag ttgtgtctga
     2521 ggacttcctc caggacgtct ccgcctccac caagagcctt caggagttgt tcttagcgca
     2581 catcttgtcc ccttgggggg cagaggtgaa ggcagagcct gttgaagttg tggccccaag
     2641 agggaagtca ggggctgcgc tctccaaaaa aagcaagggc caggtcaagg aggaaggtAT
     2701 CAACAAATCT GAAAAGAGAA TGAAATTAAC TCTTAAAGGA GGagcagctg tggatcctga
     2761 ttctggactg gaacactctg cgcatgtcct ggagaaaggt gggaaggtct tcagtgccac
     2821 ccttggcctg gtggacatcG TTAAAGGAAC CAACtccTAC TACAAGCTGC AGCTTCTGga
     2881 ggacgacaag gaaaacaggt attggatatt caggtcctgg ggcCGTgtgg gtacggtgat
     2941 cggtagcaac aaactggaac agatgccgtc caaggaggat gccattgagc acttcatgaa
     3001 attatatgaa gaaaaaaccg ggaacgcttg gcactccaaa aatttcacga agtatcccaa
     3061 aaagttctac cccctggaga ttgactatgg ccaggatgaa gaggcagtga agaagctgac
     3121 agtaaatcct ggcaccaagt ccaagctccc caagccagtt caggacctca tcaagatgat
     3181 ctttgatGTG GAAAGTATGA AGAAAGCCAT GGTGGAGTAT GAGATCgacc ttcagaagat
     3241 gcccttgggg aagctgagca aaaggcagat ccaggccgca tactccatcc tcagtgaggt
     3301 ccagcaggcg GTGTCTCAGg gcagcagcga ctctcagatc ctggatctct caaatcgctt
     3361 ttacaccctg atcccCCACG ACTTTGGGAT GAAGAAGCct ccgctcctga acaatgcaga
     3421 cagtgtgcag gccaagGTGG AAATGCTTGA CaacCTGCTG GACATCGAGG TGGCCtacag
     3481 tctgctcagg ggagggtctg atgatAGCAG Caaggatccc atcgatgtca actatgagaa
     3541 gctcaaaact gacattaagg tggttgacag agattctgaa gaagccgaga tcatcaggaa
     3601 gtatgttaag aacactcatg caaccacaca caatgcgtat gacttggaag tcatcgatat
     3661 ctttaagata gagcgtgaag gcgaatgCCA GCGTTACAAG CCCTTTAAGC agcttcataa
     3721 ccgaagattg CTGTGGCACG GGTCCaggac caccaacTTT GCTGGGATCC TGTCCCaggg
     3781 tcttCGGata gccccgcctg aagcgcccgt gacaggctac atgtttggta aagggatcta
     3841 tttcgctgac atggtctcca agagtgccaa ctactgccat acgtctcagg gagacccaat
     3901 aggcttaatc ctgttgggag aagttgccct tggaaacatg tatgaactga agcacgcttc
     3961 acatatcagc aagttaccca agggcaagca cagtgtcaaa ggtttgggca aaactacccc
     4021 tgatccttca gctaacatta gtctggatgg tgtagacgtt CCTcttGGGA CCgggatttc
     4081 atctGGTGTG AATGACACCt ctCTACTATA TAACGAGTAC ATTGtcTATG ATATTGCTCa
     4141 ggtaaatctg aagtatctgc tgaaactgaa attcaatttt aagacctccc tgtggtcGGT
     4201 ACCGCGGGCC CGGGATCCAC CGGTCGCCAC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC
     4261 CGGGGTGGTG CCCATCctgg tcgagctgga cggcgacgTA AACGGCCACA AGTTCAGCGT
     4321 GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC
     4381 CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA
     4441 GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC
     4501 CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG
     4561 CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA
     4621 CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA
     4681 CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA AGATCCGCCA
     4741 CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG
     4801 CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA
     4861 AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT
     4921 CACTCTCGGC ATGGACGAGC TGTACAAGTA AGCGGCcgcg gtggtaagaa tTCCGCCCCT
     4981 CTCCCTCCCC CCCCCCTAAC GTTACTGGCC GAAGCCGCTT GGAATAAGGC CGGTGTGCGT
     5041 TTGTCTATAT GTTATTTTCC ACCATATTGC CGTCTTTTGG CAATGTGAGG GCCCGGAAAC
     5101 CTGGCCCTGT CTTCTTGACG AGCATTCCTA GGGGTCTTTC CCCTCTCGCC AAAGGAATGC
     5161 AAGGTCTGTT GAATGTCGTG AAGGAAGCAG TTCCTCTGGA AGCTTCTTGA AGACAAACAA
     5221 CGTCTGTAGC GACCCTTTGC AGGCAGCGGA ACCCCCCACC TGGCGACAGG TGCCTCTGCG
     5281 GCCAAAAGCC ACGTGTATAA GATACACCTG CAAAGGCGGC ACAACCCCAG TGCCACGTTG
     5341 TGAGTTGGAT AGTTGTGGAA AGAGTCAAAT GGCTCTCCTC AAGCGTATTC AACAAGGGGC
     5401 TGAAGGATGC CCAGAAGGTA CCCCATTGTA TGGGATCTGA TCTGGGGCCT CGGTGCACAT
     5461 GCTTTACATG TGTTTAGTCG AGGTTAAAAA ACGTCTAGGC CCCCCGAACC ACGGGGACGT
     5521 GGTTTTCCTT TGAAAAACAC GATGATAATA TGGCCACAAC CTGTTACATT GCACAAGATA
     5581 AAAATATATC ATCACGAACA GTAAAACTGT CTGCTTACAT AAACAGTAAT ACAAGGGGTG
     5641 TTATgggatc ggccattgaa caagATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG
     5701 AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT
     5761 TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC
     5821 TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT
     5881 GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG
     5941 TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG
     6001 CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG
     6061 CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG
     6121 ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC
     6181 GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA
     6241 TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC
     6301 GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG
     6361 CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT
     6421 ATCGCCTTCT TGACGAGTTC TTCTGAAGCG GCCGCGACTC TAGATCATAA TCAGCCATAC
     6481 CACATTTGTA GAGGTTTTAC TTGCTTTAAA AAACCTCCCA CACCTCCCCC TGAACCTGAA
     6541 ACATAAAATG AATGCAATTG TTGTTGTTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA
     6601 ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG
     6661 TGGTTtgtcc aaactcatca atgtatcTTA AGGCGTAAAT TGTAAGGGTA CCAGGATCCC
     6721 TAAAGCGCAT GCTCCAGACT GNNNNNNNNN NNNNNNNNNN NNNNNNGGTC GAACGAGGAG
     6781 GTTCAAGTGC AGAAGAGCAG AGAGGATATG ctcatcgtct aaagaactac ccattttatt
     6841 atatattagt cacgatATCT ATAACAAGAA AATATATATA TAATAAgtta tcacgtaagt
     6901 agaacatgaa ataaCAATAT AATTATCGTA TGAGTTAAAT CTTAAAAGTC ACGTAAAAGA
     6961 TAATCATGCG TCATTTTGAC TCACGCGGTC GTTATAGTTC AAAATCAGTG ACACTTACCG
     7021 CATTGACAAG CACGCCTCAC GGGAGCTCCA AGCGGCGACT GAGATGTCCT AAATGCACAG
     7081 CGACGGATTC GCGCTATTTA GAAAGAGAGA GCAATATTTC AAGAATGCAT GCGTCAATTT
     7141 TACGCAGACT ATCTTTCTAG GGTTAAAAAA GATTTGCGCT TTACTCGACC TAAACTTTAA
     7201 ACACGTCATA GAATCTTCGT TTGACAAAAA CCACATTGTG GCCAAGCTGT GTGACGCGAC
     7261 GCGCGCTAAA GAATGGCAAA CCAAGTCGCG CGAGCCAGCT TTTGTTCCCT TTAGTGAGGG
     7321 TTAATTGCGC GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG
     7381 CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA
     7441 TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC
     7501 CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT
     7561 GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA
     7621 GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA
     7681 GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG
     7741 CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT
     7801 CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC
     7861 CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT
     7921 TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC
     7981 GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA
     8041 TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA
     8101 GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG
     8161 TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG
     8221 CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT
     8281 AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA
     8341 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG
     8401 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA
     8461 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA
     8521 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC
     8581 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
     8641 ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA
     8701 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT
     8761 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT
     8821 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC
     8881 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC
     8941 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA
     9001 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG
     9061 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG
     9121 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA
     9181 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA
     9241 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA
     9301 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA
     9361 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG
     9421 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT
     9481 CCCCGAAAAG TGCCACCTAA ATTGTAAGCG TTAATATTTT GTTAAAATTC GCGTTAAATT
     9541 TTTGTTAAAT CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATAAAT
     9601 CAAAAGAATA GACCGAGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT
     9661 TAAAGAACGT GGACTCCAAC GTCAAAGGGC GAAAAACCGT CTATCAGGGC GATGGCCCAC
     9721 TACGTGAACC ATCACCCTAA TCAAGTTTTT TGGGGTCGAG GTGCCGTAAA GCACTAAATC
     9781 GGAACCCTAA AGGGAGCCCC CGATTTAGAG CTTGACGGGG AAAGCCGGCG AACGTGGCGA
     9841 GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG GCGCTAGGGC GCTGGCAAGT GTAGCGGTCA
     9901 CGCTGCGCGT AACCACCACA CCCGCCGCGC TTAATGCGCC GCTACAGGGC GCGTCCCATT
     9961 CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC
    10021 GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT
    10081 CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGAGCGCGC GTAATACGAC TCACTATAGG
    10141 G     
//
