LOCUS       pPB_CMV_PARP1_AP       10879 bp    DNA        circular     02-FEB-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     gene            1154..4195
                     /gene="PARP1"
                     /gene_synonym="ADPRT; ADPRT 1; ADPRT1; ARTD1; pADPRT-1;
                     PARP; PARP-1; PPOL"
                     /note="poly (ADP-ribose) polymerase 1"
                     /db_xref="GeneID:142"
                     /db_xref="HGNC:270"
                     /db_xref="MIM:173870"
                     /locus_tag="PARP1"
                     /label="PARP1"
                     /ApEinfo_label="PARP1"
                     /ApEinfo_fwdcolor="#ffe511"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          1154..4195
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q41-q42"
                     /locus_tag="source:Homo sapiens"
                     /label="source:Homo sapiens"
                     /ApEinfo_label="source:Homo sapiens"
                     /ApEinfo_fwdcolor="#ffffff"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden=""
     CDS             6338..7184
                     /locus_tag="neo"
                     /label="neo"
                     /ApEinfo_label="neo"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             6393..7184
                     /locus_tag="Kan/neoR"
                     /label="Kan/neoR"
                     /ApEinfo_label="Kan/neoR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          4208..4954
                     /organism="pcDNA3 APEX2-NES"
                     /mol_type="other DNA"
                     /locus_tag="source:pcDNA3 APEX2-NES"
                     /label="source:pcDNA3 APEX2-NES"
                     /ApEinfo_label="source:pcDNA3 APEX2-NES"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden=""
     CDS             4208..4954
                     /translation="MDYKDDDDKGKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLM
                     LRLAFHSAGTFDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYA
                     DFYQLAGVVAVEVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLT
                     DQDIVALSGGHTIGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKA
                     LLSDPVFRPLVDKYAADEDAFFADYAEAHQKLSELGFADALQLPPLERLTLD*"
                     /locus_tag="APEX2"
                     /label="APEX2"
                     /ApEinfo_label="APEX2"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             4970..5689
                     /locus_tag="EGFP"
                     /label="EGFP"
                     /ApEinfo_label="EGFP"
                     /ApEinfo_fwdcolor="#75ff8f"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(8469..9151)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(9249..9908)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        5707..6286
                     /locus_tag="ires"
                     /label="ires"
                     /ApEinfo_label="ires"
                     /ApEinfo_fwdcolor="#ff970a"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7595..7904
                     /locus_tag="PB3"
                     /label="PB3"
                     /ApEinfo_label="PB3"
                     /ApEinfo_fwdcolor="#0cfeff"
                     /ApEinfo_revcolor="#0cfeff"
                     /ApEinfo_graphicformat="arrow_data {{} {0 1 2 0 0 -1} 0}
                     width 5 offset 0"
     rep_origin      complement(10369..10675)
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    58..320
                     /locus_tag="PB5"
                     /label="PB5"
                     /ApEinfo_label="PB5"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        739..971
                     /locus_tag="CMV"
                     /label="CMV"
                     /ApEinfo_label="CMV"
                     /ApEinfo_fwdcolor="#13ff92"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    571..738
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /locus_tag="CAG_enhancer"
                     /label="CAG_enhancer"
                     /ApEinfo_label="CAG_enhancer"
                     /ApEinfo_fwdcolor="#ff4000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        576..738
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /locus_tag="CMV_immearly_promoter"
                     /label="CMV_immearly_promoter"
                     /ApEinfo_label="CMV_immearly_promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_site      7307..7428
                     /locus_tag="SV40 polyA"
                     /label="SV40 polyA"
                     /ApEinfo_label="SV40 polyA"
                     /ApEinfo_fwdcolor="#0fff34"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(10694..10762)
                     /locus_tag="LacZ alpha"
                     /label="LacZ alpha"
                     /ApEinfo_label="LacZ alpha"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          join(1109..1140,1142..1152)
                     /organism="pBiFC-VN173"
                     /mol_type="other DNA"
                     /locus_tag="source:pBiFC-VN173"
                     /label="source:pBiFC-VN173"
                     /ApEinfo_label="source:pBiFC-VN173"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden=""
     promoter        6300..6337
                     /locus_tag="gb3"
                     /label="gb3"
                     /ApEinfo_label="gb3"
                     /ApEinfo_fwdcolor="#1014ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(509..538)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /locus_tag="lacZ_a"
                     /label="lacZ_a"
                     /ApEinfo_label="lacZ_a"
                     /ApEinfo_fwdcolor="#ff4000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     7625..7652
                     /locus_tag="PB3_seq_R"
                     /label="PB3_seq_R"
                     /ApEinfo_label="PB3_seq_R"
                     /ApEinfo_fwdcolor="#ffe609"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{} {0 1 2 0 0 -1} 0}
                     width 5 offset 0"
     misc_feature    459..483
                     /locus_tag="PB5 barcode"
                     /label="PB5 barcode"
                     /ApEinfo_label="PB5 barcode"
                     /ApEinfo_fwdcolor="#f111ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{} {} 0} width 5
                     offset 0"
     misc_feature    7480..7504
                     /locus_tag="PB3 barcode"
                     /label="PB3 barcode"
                     /ApEinfo_label="PB3 barcode"
                     /ApEinfo_fwdcolor="#dc1dff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{} {} 0} width 5
                     offset 0"
     misc_feature    1112..1135
                     /translation="MDYKDDDDKLAAANSSIDLISVPVDSRRSIATMVSKGEELFTGV
                     VPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQ
                     CFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKG
                     IDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIE*"
                     /locus_tag="FLAG"
                     /label="FLAG"
                     /ApEinfo_label="FLAG"
                     /ApEinfo_fwdcolor="#ff4000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7526..7548
                     /locus_tag="primer-right"
                     /label="primer-right"
                     /ApEinfo_label="primer-right"
                     /ApEinfo_fwdcolor="#ff0006"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    complement(8107..8129)
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    509..531
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /locus_tag="M13_pUC_fwd_primer"
                     /label="M13_pUC_fwd_primer"
                     /ApEinfo_label="M13_pUC_fwd_primer"
                     /ApEinfo_fwdcolor="#ff4000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    416..437
                     /locus_tag="primer-Left"
                     /label="primer-Left"
                     /ApEinfo_label="primer-Left"
                     /ApEinfo_fwdcolor="#ff0612"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{} {0 1 2 0 0 -1} 0}
                     width 5 offset 0"
     misc_feature    7458..7479
                     /locus_tag="PB3seqF"
                     /label="PB3seqF"
                     /ApEinfo_label="PB3seqF"
                     /ApEinfo_fwdcolor="#00ff4d"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(8081..8101)
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     10859..10879
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     4208..4228
                     /locus_tag="APEX_F"
                     /label="APEX_F"
                     /ApEinfo_label="APEX_F"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     4934..4954
                     /locus_tag="APEX2_R"
                     /label="APEX2_R"
                     /ApEinfo_label="APEX2_R"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{} {0 1 2 0 0 -1} 0}
                     width 5 offset 0"
     misc_feature    438..458
                     /locus_tag="p5+mir3"
                     /label="p5+mir3"
                     /ApEinfo_label="p5+mir3"
                     /ApEinfo_fwdcolor="#32ff82"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7505..7525
                     /locus_tag="PB3seq_R"
                     /label="PB3seq_R"
                     /ApEinfo_label="PB3seq_R"
                     /ApEinfo_fwdcolor="#11ff66"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{} {0 1 2 0 0 -1} 0}
                     width 5 offset 0"
     primer_bind     complement(8044..8063)
                     /locus_tag="T3"
                     /label="T3"
                     /ApEinfo_label="T3"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    484..502
                     /locus_tag="p7+Loop"
                     /label="p7+Loop"
                     /ApEinfo_label="p7+Loop"
                     /ApEinfo_fwdcolor="#07ffa6"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{} {0 1 2 0 0 -1} 0}
                     width 5 offset 0"
     primer_bind     10833..10850
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        524..540
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /locus_tag="M13_forward20_primer"
                     /label="M13_forward20_primer"
                     /ApEinfo_label="M13_forward20_primer"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 CGAATTGGCG GTATTCACGA CAGCAGGCTG AATAATAAAA AAATTAGAAA CTATTATTTA
       61 ACCCTAGAAA GATAATCATA TTGTGACGTA CGTTAAAGAT AATCATGCGT AAAATTGACG
      121 CATGTGTTTT ATCGGTCTGT ATATCGAGGT TTATTTATTA ATTTGAATAG ATATTAAGTT
      181 TTATTATATT TACACTTACA TACTAATAAT AAATTCAACA AACAATTTAT TTATGTTTAT
      241 TTATTTATTA AAAAAAAACA AAAACTCAAA ATTTCTTCTA TAAAGTAACA AAACTTTTAA
      301 ACATTCTCTC TTTTACAAAA ataaacttat tttgtacttt aaaaacagtc atgttgtatt
      361 ataaaataag taattagctt aacctataca taatagaaac aaattatact tattaGTCAG
      421 TCAGAAACAA CTTTGGCCTA AAGTAGCCCC TTGAATTCNN NNNNNNNNNN NNNNNNNNNN
      481 NNNTACATCT GTggcttcac tagctagcCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT
      541 GAGTTCGAGC TTGCATGCCT GCAGGTCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC
      601 TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG
      661 CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG
      721 GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA
      781 TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC
      841 ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG
      901 CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG
      961 AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA
     1021 TTGAcgcaaa tgggcggtag gcgtgTACGG TGGGAGGTCT ATATAAGCAG AGCTGGTTTA
     1081 GTGAACCGTC AGATCCgcta gcgccaccAT GGACTACAAA GACGATGACG ACAAGCTTGC
     1141 cGCCGCGAAT TCaatggcgg agtcttcgga taagctctat cgagtcgagt acgccaagag
     1201 cgggCGCgcc tcttgcaaga aaTGCAGCga gagcatcccc aaggactcgc tccggatggc
     1261 catcatggtg cagtcgccca tgTTTgatgg aaaagtccca cactggtacc acttctcctg
     1321 cttctggaag gtgggccact ccatccggca ccctgacgtt gaggtggatg ggttctctga
     1381 gcttcggtgg gatgaccagc agaaagtcaa gaagacagcg GAAgctggag gagtgacaGG
     1441 CAAAGgccag gatggaattg gtagcaaggc agagaagact ctgggtgact ttgcagcaga
     1501 gtatgccaag tccaacagaa gtacgtgcaa ggggtgtatg gagaagatag aaaagggcca
     1561 ggtgcgcctg tccaagaaga tggtggaccc ggagaagcca cagctaggca tgattgaccg
     1621 ctggtaccat ccaggctgct ttgtcaagaa cagggaggag ctgggtttcc ggcccgagta
     1681 cagtgcgagt cagctcaagg gcttcagcct ccttgctaca gaggataaag aagccctgaa
     1741 gaagcagctc ccaggagtca agagtgaagg aaagagaaaa ggcgatgagg tggatggagt
     1801 ggatgaagtg gcgaagaaga aatctaaaaa agaaaaagac aaggatagta agcttgaaaa
     1861 agccctaaag gctcagaacg acctgatctg gaacatcaag gacgagctaa agaaagtgtg
     1921 ttcaactaat gacctgaagg agctactcat cttcaacaag cagcaagtgc cttctgggga
     1981 gtcggcgatc ttggaccgag tagctgatgg catggtgttc ggtgccctcc ttccctgcga
     2041 ggaatgctcg ggtcagctgg tcttcaagag cgatgcctat tactgcactg gggacgtcac
     2101 tgcctggacc aagtgtatgg tcaaGACACA GACACCCAAC CGGAaggagt gggtaacccc
     2161 aaaggaattc cgagaaatct cttacctcaa gaaattgaag gttaaaaaac aggaccgtat
     2221 attcccccca gaaaccagcg cctccgtggc ggccacgcct ccgccctcca cagcctcggc
     2281 tcctgctgct gtgaactcct ctgcttcagc agataagcca ttatccaaca tgaagatcct
     2341 gactctcggg aagctgtccc ggaacaagga tgaagtgaag gccatgattg agaaactcgg
     2401 ggggaagttg acggggacgg ccaacaaggc ttccctgtgc atcagcacca aaaaggaggt
     2461 ggaaaagatg aataagaaga tggaggaagt aaaggaagcc aacatccgag ttgtgtctga
     2521 ggacttcctc caggacgtct ccgcctccac caagagcctt caggagttgt tcttagcgca
     2581 catcttgtcc ccttgggggg cagaggtgaa ggcagagcct gttgaagttg tggccccaag
     2641 agggaagtca ggggctgcgc tctccaaaaa aagcaagggc caggtcaagg aggaaggtat
     2701 caacaaatct gaaaagagaa tgaaattaac tcttaaagga ggagcagctg tggatcctga
     2761 ttctggactg gaacactctg cgcatgtcct ggagaaaggt gggaaggtct tcagtgccac
     2821 ccttggcctg gtggacatcg ttaaaggaac caactcctac tacaagctgc agcttctgga
     2881 ggacgacaag gaaaacaggt attggatatt caggtcctgg ggccgtgtgg gtacggtgat
     2941 cggtagcaac aaactggaac agatgccgtc caaggaggat gccattgagc acttcatgaa
     3001 attatatgaa gaaaaaaccg ggaacgcttg gcactccaaa aatttcacga agtatcccaa
     3061 aaagttctac cccctggaga ttgactatgg ccaggatgaa gaggcagtga agaagctgac
     3121 agtaaatcct ggcaccaagt ccaagctccc caagccagtt caggacctca tcaagatgat
     3181 ctttgatgtg gaaagtatga agaaagccat ggtggagtat gagatcgacc ttcagaagat
     3241 gcccttgggg aagctgagca aaaggcagat ccaggccgca tactccatcc tcagtgaggt
     3301 ccagcaggcg gtgtctcagg gcagcagcga ctctcagatc ctggatctct caaatcgctt
     3361 ttacaccctg atcccccacg actttgggat gaagaagcct ccgctcctga acaatgcaga
     3421 cagtgtgcag gccaaggtgg aaatgcttga caacctgctg gacatcgagg tggcctacag
     3481 tctgctcagg ggagggtctg atgatagcag caaggatccc atcgatgtca actatgagaa
     3541 gctcaaaact gacattaagg tggttgacag agattctgaa gaagccgaga tcatcaggaa
     3601 gtatgttaag aacactcatg caaccacaca caatgcgtat gacttggaag tcatcgatat
     3661 ctttaagata gagcgtgaag gcgaatgcca gcgttacaag ccctttaagc agcttcataa
     3721 ccgaagattg ctgtggcacg ggtccaggac caccaacttt gctgggatcc tgtcccaggg
     3781 tcttcggata gccccgcctg aagcgcccgt gacaggctac atgtttggta aagggatcta
     3841 tttcgctgac atggtctcca agagtgccaa ctactgccat acgtctcagg gagacccaat
     3901 aggcttaatc ctgttgggag aagttgccct tggaaacatg tatgaactga agcacgcttc
     3961 acatatcagc aagttaccca agggcaagca cagtgtcaaa ggtttgggca aaactacccc
     4021 tgatccttca gctaacatta gtctggatgg tgtagacgtt cctcttggga ccgggatttc
     4081 atctggtgtg aatgacacct ctctactaTA Taacgagtac attgtcTATG ATATTGCTCa
     4141 ggtaaatctg aagtatctgc tgaaactgaa attcaatttt aagacctccc tgtggtcggt
     4201 accgcgggga aagtcttacc caACTGTGAG TGCTGATTAC CAGGACGCCG TTGAGAAGGC
     4261 GAAGAAGAAG CTCAGAGGCT TCATCGCTGA GAAGAGATGC GCTCCTCTAA TGCTCCGTTT
     4321 GGCATTCCAC TCTGCTGGAA CCTTTGACAA GGGCACGAAG ACCGGTGGAC CCTTCGGAAC
     4381 CATCAAGCAC CCTGCCGAAC TGGCTCACAG CGCTAACAAC GGTCTTGACA TCGCTGTTAG
     4441 GCTTTTGGAG CCACTCAAGG CGGAGTTCCC TATTTTGAGC TACGCCGATT TCTACCAGTT
     4501 GGCTGGCGTT GTTGCCGTTG AGGTCACGGG TGGACCTAAG GTTCCATTCC ACCCTGGAAG
     4561 AGAGGACAAG CCTGAGCCAC CACCAGAGGG TCGCTTGCCC GATCCCACTA AGGGTTCTGA
     4621 CCATTTGAGA GATGTGTTTG GCAAAGCTAT GGGGCTTACT GACCAAGATA TCGTTGCTCT
     4681 ATCTGGGGGT CACACTATTG GAGCTGCACA CAAGGAGCGT TCTGGATTTG AGGGTCCCTG
     4741 GACCTCTAAT CCTCTTATTT TCGACAACTC ATACTTCACG GAGTTGTTGA GTGGTGAGAA
     4801 GGAAGGTCTC CTTCAGCTAC CTTCTGACAA GGCTCTTTTG TCTGACCCTG TATTCCGCCC
     4861 TCTCGTTGAC AAATATGCAG CGGACGAAGA TGCCTTCTTT GCTGATTACG CTGAGGCTCA
     4921 CCAAAAGCTT TCCGAGCTTg ggtttgctga tgccccaccg gtcgccacca tggtgAGCAA
     4981 GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCctggtc gagctggacg gcgacgTAAA
     5041 CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC
     5101 CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC
     5161 CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT
     5221 CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA
     5281 CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT
     5341 CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA
     5401 CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT
     5461 GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA
     5521 GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC
     5581 CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT
     5641 CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGTAAG CGGCcgcggt
     5701 ggtaagaatT CCGCCCCTCT CCCTCCCCCC CCCCTAACGT TACTGGCCGA AGCCGCTTGG
     5761 AATAAGGCCG GTGTGCGTTT GTCTATATGT TATTTTCCAC CATATTGCCG TCTTTTGGCA
     5821 ATGTGAGGGC CCGGAAACCT GGCCCTGTCT TCTTGACGAG CATTCCTAGG GGTCTTTCCC
     5881 CTCTCGCCAA AGGAATGCAA GGTCTGTTGA ATGTCGTGAA GGAAGCAGTT CCTCTGGAAG
     5941 CTTCTTGAAG ACAAACAACG TCTGTAGCGA CCCTTTGCAG GCAGCGGAAC CCCCCACCTG
     6001 GCGACAGGTG CCTCTGCGGC CAAAAGCCAC GTGTATAAGA TACACCTGCA AAGGCGGCAC
     6061 AACCCCAGTG CCACGTTGTG AGTTGGATAG TTGTGGAAAG AGTCAAATGG CTCTCCTCAA
     6121 GCGTATTCAA CAAGGGGCTG AAGGATGCCC AGAAGGTACC CCATTGTATG GGATCTGATC
     6181 TGGGGCCTCG GTGCACATGC TTTACATGTG TTTAGTCGAG GTTAAAAAAC GTCTAGGCCC
     6241 CCCGAACCAC GGGGACGTGG TTTTCCTTTG AAAAACACGA TGATAATATG GCCACAACCT
     6301 GTTACATTGC ACAAGATAAA AATATATCAT CACGAACAGT AAAACTGTCT GCTTACATAA
     6361 ACAGTAATAC AAGGGGTGTT ATgggatcgg ccattgaaca agATGGATTG CACGCAGGTT
     6421 CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT
     6481 GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA
     6541 CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG
     6601 CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT
     6661 GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG
     6721 AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT
     6781 GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG
     6841 GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT
     6901 TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG
     6961 CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC
     7021 GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG
     7081 AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT
     7141 CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAAGCGGC CGCGACTCTA
     7201 GATCATAATC AGCCATACCA CATTTGTAGA GGTTTTACTT GCTTTAAAAA ACCTCCCACA
     7261 CCTCCCCCTG AACCTGAAAC ATAAAATGAA TGCAATTGTT GTTGTTAACT TGTTTATTGC
     7321 AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT
     7381 TTCACTGCAT TCTAGTTGTG GTTtgtccaa actcatcaat gtatcTTAAG GCGTAAATTG
     7441 TAAGGGTACC AGGATCCCTA AAGCGCATGC TCCAGACTGN NNNNNNNNNN NNNNNNNNNN
     7501 NNNNGGTCGA ACGAGGAGGT TCAAGTGCAG AAGAGCAGAG AGGATATGct catcgtctaa
     7561 agaactaccc attttattat atattagtca cgatATCTAT AACAAGAAAA TATATATATA
     7621 ATAAgttatc acgtaagtag aacatgaaat aaCAATATAA TTATCGTATG AGTTAAATCT
     7681 TAAAAGTCAC GTAAAAGATA ATCATGCGTC ATTTTGACTC ACGCGGTCGT TATAGTTCAA
     7741 AATCAGTGAC ACTTACCGCA TTGACAAGCA CGCCTCACGG GAGCTCCAAG CGGCGACTGA
     7801 GATGTCCTAA ATGCACAGCG ACGGATTCGC GCTATTTAGA AAGAGAGAGC AATATTTCAA
     7861 GAATGCATGC GTCAATTTTA CGCAGACTAT CTTTCTAGGG TTAAAAAAGA TTTGCGCTTT
     7921 ACTCGACCTA AACTTTAAAC ACGTCATAGA ATCTTCGTTT GACAAAAACC ACATTGTGGC
     7981 CAAGCTGTGT GACGCGACGC GCGCTAAAGA ATGGCAAACC AAGTCGCGCG AGCCAGCTTT
     8041 TGTTCCCTTT AGTGAGGGTT AATTGCGCGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT
     8101 GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT
     8161 AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC
     8221 GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG
     8281 AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG
     8341 GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA
     8401 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC
     8461 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC
     8521 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG
     8581 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC
     8641 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT
     8701 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG
     8761 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC
     8821 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT
     8881 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT
     8941 ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC
     9001 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA
     9061 AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC
     9121 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC
     9181 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT
     9241 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA
     9301 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT
     9361 GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA
     9421 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC
     9481 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG
     9541 CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT
     9601 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA
     9661 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA
     9721 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC
     9781 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG
     9841 AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA
     9901 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG
     9961 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC
    10021 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG
    10081 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT
    10141 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA
    10201 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTAAAT TGTAAGCGTT AATATTTTGT
    10261 TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACCAATAG GCCGAAATCG
    10321 GCAAAATCCC TTATAAATCA AAAGAATAGA CCGAGATAGG GTTGAGTGTT GTTCCAGTTT
    10381 GGAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAACCGTCT
    10441 ATCAGGGCGA TGGCCCACTA CGTGAACCAT CACCCTAATC AAGTTTTTTG GGGTCGAGGT
    10501 GCCGTAAAGC ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA
    10561 AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC
    10621 TGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC CGCCGCGCTT AATGCGCCGC
    10681 TACAGGGCGC GTCCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC
    10741 GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT
    10801 GGGTAACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAGCGCGCGT
    10861 AATACGACTC ACTATAGGG    
//
