LOCUS       Exported                2702 bp ds-DNA     circular SYN 28-OCT-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Oct 30, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    235..329
                     /locus_tag="CP"
                     /label=CP
     misc_feature    235..240
                     /locus_tag="NarI (Backbone/Promoter)"
                     /label=NarI (Backbone/Promoter)
     regulatory      241..329
                     /regulatory_class="promoter"
                     /locus_tag="Promoter (Miller 1986)"
                     /label=Promoter (Miller 1986)
     misc_feature    271..276
                     /locus_tag="NarI*"
                     /label=NarI*
     regulatory      291..296
                     /regulatory_class="minus_35_signal"
                     /locus_tag="-35"
                     /label=-35
     regulatory      314..318
                     /regulatory_class="minus_10_signal"
                     /locus_tag="-10"
                     /label=-10
     regulatory      327
                     /regulatory_class="other"
                     /locus_tag="-1"
                     /label=-1
     misc_feature    337..342
                     /locus_tag="EcoRI (Promoter/tRNA)"
                     /label=EcoRI (Promoter/tRNA)
     tRNA            343..418
                     /locus_tag="Ala"
                     /label=Ala
     misc_feature    426..431
                     /locus_tag="PstI (tRNA/terminator)"
                     /label=PstI (tRNA/terminator)
     regulatory      432..460
                     /regulatory_class="terminator"
                     /locus_tag="Terminator (Normanly 1986)"
                     /label=Terminator (Normanly 1986)
     misc_feature    461..466
                     /locus_tag="HindIII (terminator/backbone)"
                     /label=HindIII (terminator/backbone)
     primer_bind     complement(477..497)
                     /locus_tag="M13-rev"
                     /PCR_conditions="primer sequence:CAGGAAACAGCTATGACCATG"
                     /label=M13-rev
     misc_binding    complement(503..525)
                     /locus_tag="LacO"
                     /label=LacO
     rep_origin      complement(865..1547)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     CDS             complement(1645..2304)
                     /locus_tag="AmpR"
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 gcttctttga gcgaacgatc aaaaataagt ggggccccat caaaaaaata ttctcaacat
      301 aaaaaacttt gtgtaatact tgtaacgctg aattcagaat tcggggctat agctcagctg
      361 ggagagcgct tgcatctaaa gcaagaggtc agcggttcga tcccgcttag ctccaccact
      421 gcagtctgca gatccttagc gaaagctaag gatttttttt aagcttcggg cgtaatcatg
      481 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
      541 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
      601 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
      661 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
      721 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      781 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
      841 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
      901 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      961 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1021 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     1081 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1141 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1201 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     1261 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     1321 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     1381 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     1441 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     1501 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     1561 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     1621 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     1681 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     1741 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     1801 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     1861 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     1921 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     1981 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     2041 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     2101 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     2161 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     2221 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     2281 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     2341 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     2401 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     2461 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     2521 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     2581 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     2641 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     2701 tc
//
