LOCUS       Exported                2687 bp ds-DNA     circular SYN 28-OCT-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Oct 30, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    235..240
                     /locus_tag="NarI (Backbone/Promoter)"
                     /label=NarI (Backbone/Promoter)
     regulatory      241..329
                     /regulatory_class="promoter"
                     /locus_tag="Promoter (Miller 1986)"
                     /label=Promoter (Miller 1986)
     misc_feature    271..276
                     /locus_tag="NarI*"
                     /label=NarI*
     regulatory      291..296
                     /regulatory_class="minus_35_signal"
                     /locus_tag="-35"
                     /label=-35
     regulatory      314..318
                     /regulatory_class="minus_10_signal"
                     /locus_tag="-10"
                     /label=-10
     regulatory      327
                     /regulatory_class="other"
                     /locus_tag="-1"
                     /label=-1
     misc_feature    330..335
                     /locus_tag="EcoRI (Promoter/tRNA)"
                     /label=EcoRI (Promoter/tRNA)
     tRNA            336..409
                     /locus_tag="Cys"
                     /label=Cys
     misc_feature    410..415
                     /locus_tag="PstI (tRNA/terminator)"
                     /label=PstI (tRNA/terminator)
     regulatory      417..445
                     /regulatory_class="terminator"
                     /locus_tag="Terminator (Normanly 1986)"
                     /label=Terminator (Normanly 1986)
     misc_feature    446..451
                     /locus_tag="HindIII (terminator/backbone)"
                     /label=HindIII (terminator/backbone)
     primer_bind     complement(462..482)
                     /locus_tag="M13-rev"
                     /PCR_conditions="primer sequence:CAGGAAACAGCTATGACCATG"
                     /label=M13-rev
     misc_binding    complement(488..510)
                     /locus_tag="LacO"
                     /label=LacO
     rep_origin      complement(850..1532)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     CDS             complement(1630..2289)
                     /locus_tag="AmpR"
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 gcttctttga gcgaacgatc aaaaataagt ggggccccat caaaaaaata ttctcaacat
      301 aaaaaacttt gtgtaatact tgtaacgctg aattcggcgc gttaacaaag cggttatgta
      361 gcggattcta aatccgtcta gtccggttcg actccggaac gcgcctccac tgcagtatcc
      421 ttagcgaaag ctaaggattt tttttaagct tcgggcgtaa tcatggtcat agctgtttcc
      481 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
      541 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
      601 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
      661 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
      721 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      781 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      841 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
      901 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      961 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1021 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1081 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1141 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1201 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1261 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1321 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1381 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1441 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1501 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     1561 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     1621 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     1681 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     1741 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     1801 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     1861 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     1921 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     1981 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     2041 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     2101 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     2161 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     2221 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     2281 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     2341 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     2401 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     2461 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     2521 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     2581 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     2641 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtc
//
