LOCUS       Exported                2688 bp ds-DNA     circular SYN 28-OCT-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Oct 30, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    235..240
                     /locus_tag="NarI (Backbone/Promoter)"
                     /label=NarI (Backbone/Promoter)
     regulatory      241..329
                     /regulatory_class="promoter"
                     /locus_tag="Promoter (Miller 1986)"
                     /label=Promoter (Miller 1986)
     misc_feature    271..276
                     /locus_tag="NarI*"
                     /label=NarI*
     regulatory      291..296
                     /regulatory_class="minus_35_signal"
                     /locus_tag="-35"
                     /label=-35
     regulatory      314..318
                     /regulatory_class="minus_10_signal"
                     /locus_tag="-10"
                     /label=-10
     regulatory      327
                     /regulatory_class="other"
                     /locus_tag="-1"
                     /label=-1
     misc_feature    331..336
                     /locus_tag="EcoRI (Promoter/tRNA)"
                     /label=EcoRI (Promoter/tRNA)
     tRNA            337..411
                     /locus_tag="Gln"
                     /label=Gln
     misc_feature    412..417
                     /locus_tag="PstI (tRNA/terminator)"
                     /label=PstI (tRNA/terminator)
     regulatory      418..446
                     /regulatory_class="terminator"
                     /locus_tag="Terminator (Normanly 1986)"
                     /label=Terminator (Normanly 1986)
     misc_feature    447..452
                     /locus_tag="HindIII (terminator/backbone)"
                     /label=HindIII (terminator/backbone)
     primer_bind     complement(463..483)
                     /locus_tag="M13-rev"
                     /PCR_conditions="primer sequence:CAGGAAACAGCTATGACCATG"
                     /label=M13-rev
     misc_binding    complement(489..511)
                     /locus_tag="LacO"
                     /label=LacO
     rep_origin      complement(851..1533)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     CDS             complement(1631..2290)
                     /locus_tag="AmpR"
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 gcttctttga gcgaacgatc aaaaataagt ggggccccat caaaaaaata ttctcaacat
      301 aaaaaacttt gtgtaatact tgtaacgcta gaattctggg gtatcgccaa gcggtaaggc
      361 accggattct aaatccggca ttccgaggtt cgaatcctcg taccccagcc actgcagatc
      421 cttagcgaaa gctaaggatt ttttttaagc ttcgggcgta atcatggtca tagctgtttc
      481 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
      541 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
      601 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
      661 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
      721 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
      781 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
      841 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
      901 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      961 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1021 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1081 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1141 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1201 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1261 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     1321 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     1381 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     1441 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     1501 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     1561 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     1621 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     1681 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     1741 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     1801 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     1861 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     1921 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     1981 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     2041 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     2101 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     2161 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     2221 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     2281 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     2341 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     2401 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     2461 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     2521 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     2581 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     2641 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc
//
