LOCUS       Exported                2698 bp ds-DNA     circular SYN 28-OCT-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Oct 30, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    235..240
                     /locus_tag="NarI (Backbone/Promoter)"
                     /label=NarI (Backbone/Promoter)
     regulatory      241..329
                     /regulatory_class="promoter"
                     /locus_tag="Promoter (Miller 1986)"
                     /label=Promoter (Miller 1986)
     misc_feature    271..276
                     /locus_tag="NarI*"
                     /label=NarI*
     regulatory      291..296
                     /regulatory_class="minus_35_signal"
                     /locus_tag="-35"
                     /label=-35
     regulatory      314..318
                     /regulatory_class="minus_10_signal"
                     /locus_tag="-10"
                     /label=-10
     regulatory      327
                     /regulatory_class="other"
                     /locus_tag="-1"
                     /label=-1
     misc_feature    330..335
                     /locus_tag="EcoRI (Promoter/tRNA)"
                     /label=EcoRI (Promoter/tRNA)
     tRNA            336..420
                     /locus_tag="Leu"
                     /label=Leu
     misc_feature    421..426
                     /locus_tag="PstI (tRNA/terminator)"
                     /label=PstI (tRNA/terminator)
     regulatory      428..456
                     /regulatory_class="terminator"
                     /locus_tag="Terminator (Normanly 1986)"
                     /label=Terminator (Normanly 1986)
     misc_feature    457..462
                     /locus_tag="HindIII (terminator/backbone)"
                     /label=HindIII (terminator/backbone)
     primer_bind     complement(473..493)
                     /locus_tag="M13-rev"
                     /PCR_conditions="primer sequence:CAGGAAACAGCTATGACCATG"
                     /label=M13-rev
     misc_binding    complement(499..521)
                     /locus_tag="LacO"
                     /label=LacO
     rep_origin      complement(861..1543)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     CDS             complement(1641..2300)
                     /locus_tag="AmpR"
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 gcttctttga gcgaacgatc aaaaataagt ggggccccat caaaaaaata ttctcaacat
      301 aaaaaacttt gtgtaatact tgtaacgctg aattcgccga agtggngaaa tcggtagacg
      361 cagttgattc taaatcaacc gtagaaatac gtgccggttc gagtccggcc ttcggcacca
      421 ctgcagtatc cttagcgaaa gctaaggatt ttttttaagc ttcgggcgta atcatggtca
      481 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
      541 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
      601 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
      661 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
      721 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
      781 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
      841 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
      901 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
      961 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1021 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1081 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1141 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1201 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1261 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     1321 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1381 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1441 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1501 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     1561 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     1621 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     1681 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     1741 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     1801 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     1861 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     1921 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     1981 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2041 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2101 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2161 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     2221 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     2281 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     2341 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     2401 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     2461 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     2521 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     2581 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     2641 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc
//
