LOCUS       Exported                2689 bp ds-DNA     circular SYN 28-OCT-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Oct 30, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     >Lys-1 is the first Lysine construct
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    235..240
                     /locus_tag="NarI (Backbone/Promoter)"
                     /label=NarI (Backbone/Promoter)
     regulatory      241..329
                     /regulatory_class="promoter"
                     /locus_tag="Promoter (Miller 1986)"
                     /label=Promoter (Miller 1986)
     misc_feature    271..276
                     /locus_tag="NarI*"
                     /label=NarI*
     regulatory      291..296
                     /regulatory_class="minus_35_signal"
                     /locus_tag="-35"
                     /label=-35
     regulatory      314..318
                     /regulatory_class="minus_10_signal"
                     /locus_tag="-10"
                     /label=-10
     regulatory      327
                     /regulatory_class="other"
                     /locus_tag="-1"
                     /label=-1
     misc_feature    330..335
                     /locus_tag="EcoRI (Promoter/tRNA)"
                     /label=EcoRI (Promoter/tRNA)
     tRNA            336..411
                     /locus_tag="Lys1"
                     /label=Lys1
     misc_feature    412..417
                     /locus_tag="PstI (tRNA/terminator)"
                     /label=PstI (tRNA/terminator)
     regulatory      419..447
                     /regulatory_class="terminator"
                     /locus_tag="Terminator (Normanly 1986)"
                     /label=Terminator (Normanly 1986)
     misc_feature    448..453
                     /locus_tag="HindIII (terminator/backbone)"
                     /label=HindIII (terminator/backbone)
     primer_bind     complement(464..484)
                     /locus_tag="M13-rev"
                     /PCR_conditions="primer sequence:CAGGAAACAGCTATGACCATG"
                     /label=M13-rev
     misc_binding    complement(490..512)
                     /locus_tag="LacO"
                     /label=LacO
     rep_origin      complement(852..1534)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     CDS             complement(1632..2291)
                     /locus_tag="AmpR"
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 gcttctttga gcgaacgatc aaaaataagt ggggccccat caaaaaaata ttctcaacat
      301 aaaaaacttt gtgtaatact tgtaacgctg aattcgggtc gttagctcag ttggtagagc
      361 agttgactct aaatcaattg gtcgcaggtt cgaatcctgc acgacccacc actgcagtat
      421 ccttagcgaa agctaaggat tttttttaag cttcgggcgt aatcatggtc atagctgttt
      481 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
      541 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
      601 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
      661 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
      721 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
      781 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
      841 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
      901 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
      961 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1021 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1081 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1141 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1201 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1261 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     1321 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1381 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1441 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     1501 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     1561 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     1621 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     1681 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     1741 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     1801 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     1861 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     1921 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     1981 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     2041 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     2101 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     2161 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     2221 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     2281 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     2341 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     2401 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     2461 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     2521 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     2581 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     2641 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc
//
