LOCUS       Exported               10026 bp ds-DNA     circular SYN 03-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10026)
  AUTHORS   Ryan Lister
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Nov 1, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10026
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        3..229
                     /label=RSV promoter
                     /note="/ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green"
     LTR             230..410
                     /label=5'LTR (truncated)
                     /note="/ApEinfo_fwdcolor=#800040
                     /ApEinfo_revcolor=#800040"
     misc_feature    457..582
                     /label=HIV-1 psi
                     /note="/ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=#cccccc"
     misc_structure  1075..1308
                     /label=RRE
                     /note="/ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=#ffff00"
     promoter        1816..2322
                     /label=CMV promoter
                     /note="/ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green"
     primer_bind     2286..2305
                     /label=TOL599_pLenti-BSD_seq_F
     primer_bind     2341..2391
                     /label=TOL683_mCherry_gRNA_pLenti_F
     CDS             2371..3081
                     /label=mCherry
     primer_bind     2374..2391
                     /label=TOL94_attB_mCherry_F
     primer_bind     2782..2801
                     /label=OL-D282
     primer_bind     complement(3057..3078)
                     /label=TOL95_attB_mCherry_R
     intron          3120..3349
                     /label=chimeric intron
     primer_bind     3364..3393
                     /label=TOL630_SV40_EcoRI_F
     polyA_signal    3528..3649
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     3528..3567
                     /label=TOL577_3xKET-gRNA_F
     primer_bind     complement(3635..3670)
                     /label=TOL631_SV40_PspXI_R
     primer_bind     3640..3699
                     /label=TOL701_PspXI_gRNA_F
     primer_bind     complement(3650..3662)
                     /label=TOL55_mCherry_PBS_R
     promoter        3670..3966
                     /label=U6 Promoter
     primer_bind     3670..3685
                     /label=TOL532_gRNA_insert1_F
     primer_bind     3739..3764
                     /label=TOL271_gRNA_colonyPCR_F
     misc_feature    3988..4007
                     /label=HINT1-1-sgRNA
     misc_feature    4030..4084
                     /label=gRNA Scaffold
                     
     primer_bind     complement(4057..4082)
                     /label=TOL272_gRNA_colonyPCR_R
     primer_bind     complement(4073..4094)
                     /label=TOL702_PspXI_gRNA_R
     primer_bind     complement(4074..4099)
                     /label=TOL633_gRNA_R
     primer_bind     complement(4090..4109)
                     /label=TOL531_gRNA_backbone_R
     primer_bind     complement(4091..4111)
                     /label=TOL533_gRNA_insert1_R
     primer_bind     complement(4095..4151)
                     /label=TOL711_PspXI_gRNA_R2
     primer_bind     complement(4101..4118)
                     /label=TOL535_gRNA_insert2_R
     primer_bind     4119..4150
                     /label=TOL701_PspXI_gRNA_F
     promoter        4121..4417
                     /label=U6 Promoter
     primer_bind     4121..4136
                     /label=TOL532_gRNA_insert1_F
     primer_bind     4190..4215
                     /label=TOL271_gRNA_colonyPCR_F
     primer_bind     4399..4458
                     /label=TOL649_RBM3-1-gRNA_F
     primer_bind     complement(4439..4498)
                     /label=TOL650_RBM3-1-gRNA_R
     misc_feature    4439..4458
                     /label=RBM3-1-sgRNA
     misc_feature    4481..4535
                     /label=gRNA Scaffold
                     
     primer_bind     complement(4508..4533)
                     /label=TOL272_gRNA_colonyPCR_R
     primer_bind     complement(4524..4574)
                     /label=TOL702_PspXI_gRNA_R
     primer_bind     complement(4525..4566)
                     /label=TOL633_gRNA_R
     primer_bind     4545..4584
                     /label=TOL648_pU6_PspXI_F2
     primer_bind     4560..4588
                     /label=TOL534_gRNA_insert2_F
     primer_bind     4572..4607
                     /label=TOL532_gRNA_insert1_F
     promoter        4592..4888
                     /label=U6 Promoter
     primer_bind     4592..4621
                     /label=TOL701_PspXI_gRNA_F
     primer_bind     4661..4686
                     /label=TOL271_gRNA_colonyPCR_F
     primer_bind     4869..4928
                     /label=TOL334_B2M.4_sgRNA_F2
     primer_bind     complement(4910..4969)
                     /label=TOL335_B2M.4_sgRNA_R2
     misc_feature    4910..4928
                     /label=B2M.4-sgRNA
     misc_feature    4950..5005
                     /label=gRNA Scaffold
                     
     primer_bind     complement(4978..5003)
                     /label=TOL272_gRNA_colonyPCR_R
     primer_bind     complement(4994..5058)
                     /label=TOL702_PspXI_gRNA_R
     primer_bind     complement(4995..5050)
                     /label=TOL633_gRNA_R
     primer_bind     5014..5068
                     /label=TOL648_pU6_PspXI_F2
     primer_bind     5044..5072
                     /label=TOL534_gRNA_insert2_F
     primer_bind     5056..5091
                     /label=TOL532_gRNA_insert1_F
     promoter        5076..5372
                     /label=U6 Promoter
     primer_bind     5076..5105
                     /label=TOL701_PspXI_gRNA_F
     primer_bind     5145..5170
                     /label=TOL271_gRNA_colonyPCR_F
     primer_bind     5353..5412
                     /label=TOL344_KL-3-sgRNA_F
     misc_feature    5394..5412
                     /label=KL-3-sgRNA
     misc_feature    5435..5489
                     /label=gRNA Scaffold
     primer_bind     complement(5462..5487)
                     /label=TOL272_gRNA_colonyPCR_R
     primer_bind     complement(5478..5499)
                     /label=TOL702_PspXI_gRNA_R
     primer_bind     complement(5479..5504)
                     /label=TOL633_gRNA_R
     misc_feature    5489..5495
                     /label=poly T terminator
     primer_bind     complement(5495..5515)
                     /label=TOL531_gRNA_backbone_R
     primer_bind     complement(5496..5514)
                     /label=TOL533_gRNA_insert1_R
     primer_bind     complement(5500..5564)
                     /label=TOL711_PspXI_gRNA_R2
     primer_bind     5512..5549
                     /label=TOL532_gRNA_insert1_F
     primer_bind     5514..5530
                     /label=TOL534_gRNA_insert2_F
     promoter        5534..5830
                     /label=U6 Promoter
     primer_bind     5534..5563
                     /label=TOL701_PspXI_gRNA_F
     primer_bind     5603..5628
                     /label=TOL271_gRNA_colonyPCR_F
     primer_bind     5811..5870
                     /label=TOL546_EpCAM-2-sgRNA_F
     primer_bind     complement(5852..5895)
                     /label=TOL547_EpCAM-2-sgRNA_R
     misc_feature    5852..5870
                     /label=EpCAM-2-sgRNA
     misc_feature    5893..5947
                     /label=gRNA Scaffold
     primer_bind     complement(5920..5945)
                     /label=TOL272_gRNA_colonyPCR_R
     primer_bind     complement(5936..5989)
                     /label=TOL702_PspXI_gRNA_R
     primer_bind     complement(5937..5962)
                     /label=TOL633_gRNA_R
     misc_feature    5947..5953
                     /label=poly T terminator
     primer_bind     complement(5953..5972)
                     /label=TOL531_gRNA_backbone_R
     primer_bind     complement(5954..5976)
                     /label=TOL533_gRNA_insert1_R
     primer_bind     complement(5958..6037)
                     /label=TOL711_PspXI_gRNA_R2
     primer_bind     5972..6003
                     /label=TOL534_gRNA_insert2_F
     primer_bind     5975..5999
                     /label=TOL648_pU6_PspXI_F2
     primer_bind     5987..6022
                     /label=TOL532_gRNA_insert1_F
     promoter        6007..6303
                     /label=U6 Promoter
     primer_bind     6007..6036
                     /label=TOL701_PspXI_gRNA_F
     primer_bind     6076..6101
                     /label=TOL271_gRNA_colonyPCR_F
     primer_bind     6283..6342
                     /label=TOL336_TMEM206.2-sgRNA_F
     primer_bind     complement(6325..6384)
                     /label=TOL337_TMEM206.2-sgRNA_R
     misc_feature    6325..6342
                     /label=TMEM206-2-sgRNA
     misc_feature    6365..6419
                     /label=gRNA Scaffold
     primer_bind     complement(6392..6417)
                     /label=TOL272_gRNA_colonyPCR_R
     primer_bind     complement(6408..6429)
                     /label=TOL702_PspXI_gRNA_R
     primer_bind     complement(6409..6434)
                     /label=TOL633_gRNA_R
     misc_feature    6419..6425
                     /label=poly T terminator
     primer_bind     complement(6425..6444)
                     /label=TOL531_gRNA_backbone_R
     primer_bind     complement(6426..6446)
                     /label=TOL533_gRNA_insert1_R
     primer_bind     complement(6430..6453)
                     /label=TOL711_PspXI_gRNA_R2
     primer_bind     complement(6436..6459)
                     /label=TOL535_gRNA_insert2_R
     primer_bind     complement(6447..6458)
                     /label=TOL598_3xgRNA_seq_R
     primer_bind     complement(6451..6508)
                     /label=TOL684_mCherry_gRNA_pLenti_R
     primer_bind     complement(6458..6508)
                     /label=TOL578
     primer_bind     complement(6462..6478)
                     /label=TOL598_3xgRNA_seq_R
     primer_bind     complement(6477..6478)
                     /label=M13 rev
     LTR             6547..6780
                     /label=3'LTR (deltaUT)
                     /note="/ApEinfo_fwdcolor=#800040
                     /ApEinfo_revcolor=#800040"
     polyA_site      6852..6973
                     /label=SV40 poly(A)
                     /note="/ApEinfo_fwdcolor=#800080
                     /ApEinfo_revcolor=#800080"
     primer_bind     6852..6891
                     /label=TOL577_3xKET-gRNA_F
     primer_bind     6940..6978
                     /label=TOL648_pU6_PspXI_F2
     primer_bind     6954..6982
                     /label=TOL534_gRNA_insert2_F
     primer_bind     complement(7168..7188)
                     /label=T7
                     /note="/ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green"
     primer_bind     complement(7197..7214)
                     /label=M13-fwd
                     /note="/ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green"
     CDS             7285..7353
                     /label=LacZ alpha
                     /note="/ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed"
     rep_origin      7372..7678
                     /label=F1 ori
                     /note="/ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50"
     CDS             8139..8798
                     /label=AmpR
                     /note="/ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow"
     rep_origin      8896..9578
                     /label=ColE1 origin
                     /note="/ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50"
     misc_binding    9918..9940
                     /label=LacO
                     /note="/ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed"
     primer_bind     9946..9966
                     /label=M13-rev
                     /note="/ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green"
     primer_bind     9948..9977
                     /label=TOL598_3xgRNA_seq_R
     primer_bind     9984..10003
                     /label=T3
                     /note="/ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgata
     1801 agcttgggag ttccgcttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     1861 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     1921 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     1981 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     2041 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     2101 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     2161 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     2221 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     2281 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctccctat cagtgataga
     2341 gatctcccta tcagtgatag agatcgtcga atggtgagca agggcgagga ggataacatg
     2401 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
     2461 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     2521 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
     2581 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
     2641 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     2701 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
     2761 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     2821 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
     2881 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
     2941 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
     3001 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
     3061 atggacgagc tgtacaagtg atggaattaa ttcgctgtct gcgagggcca gctgttgggg
     3121 tgagtactcc ctctcaaaag cgggcatgac ttctgcgcta agattgtcag tttccaaaaa
     3181 cgaggaggat ttgatattca cctggcccgc ggtgatgcct ttgagggtgg ccgcgtccat
     3241 ctggtcagaa aagacaatct ttttgttgtc aagcttgagg tgtggcaggc ttgagatctg
     3301 gccatacact tgagtgacaa tgacatccac tttgcctttc tctccacagg tgtccactcc
     3361 caggtccaac tgcaggtcga tttaaatggg aattccacag cggccgcatg tctagataac
     3421 tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     3481 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     3541 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     3601 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa tgcgtcgagt
     3661 gcctcgaggt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac tggatccggt
     3721 accaaggtcg ggcaggaaga gggcctattt cccatgattc cttcatattt gcatatacga
     3781 tacaaggctg ttagagagat aattagaatt aatttgactg taaacacaaa gatattagta
     3841 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
     3901 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
     3961 tatatcttgt ggaaaggacg aaacaccgac caggaacttc cccaagcgtt ttagagctag
     4021 aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
     4081 tgcttttttt accgtcgacc tctagctaga gcttggcggg tgtacaaaaa agcaggcttt
     4141 aaaggaacca attcagtcga ctggatccgg taccaaggtc gggcaggaag agggcctatt
     4201 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat
     4261 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
     4321 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt
     4381 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccga
     4441 agacatccga tccagcgggt tttagagcta gaaatagcaa gttaaaataa ggctagtccg
     4501 ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt taccggttgt ggtttgtcca
     4561 aactcatcaa tgtatcttat catgtctgta ttgtacaaaa aagcaggctt taaaggaacc
     4621 aattcagtcg actggatccg gtaccaaggt cgggcaggaa gagggcctat ttcccatgat
     4681 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac
     4741 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
     4801 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa
     4861 gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg ggtaggagag
     4921 actcacgcgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt
     4981 gaaaaagtgg caccgagtcg gtgctttttt taccgtcgac tgcattctag ttgtggtttg
     5041 tccaaactca tcaatgtatc ttatcatgtc tgtattgtac aaaaaagcag gctttaaagg
     5101 aaccaattca gtcgactgga tccggtacca aggtcgggca ggaagagggc ctatttccca
     5161 tgattccttc atatttgcat atacgataca aggctgttag agagataatt agaattaatt
     5221 tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg
     5281 ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt
     5341 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac accggaggca
     5401 gtcccggctc gcgttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
     5461 acttgaaaaa gtggcaccga gtcggtgctt tttttaccgt cgacctctag ctagtatctt
     5521 atcatgtctg tattgtacaa aaaagcaggc tttaaaggaa ccaattcagt cgactggatc
     5581 cggtaccaag gtcgggcagg aagagggcct atttcccatg attccttcat atttgcatat
     5641 acgatacaag gctgttagag agataattag aattaatttg actgtaaaca caaagatatt
     5701 agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt
     5761 atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc
     5821 tttatatatc ttgtggaaag gacgaaacac cgggagttgg gggagtgagt gttttagagc
     5881 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
     5941 cggtgctttt tttaccgtcg acctctagct agagaaactc atcaatgtat cttatcatgt
     6001 ctgtattgta caaaaaagca ggctttaaag gaaccaattc agtcgactgg atccggtacc
     6061 aaggtcgggc aggaagaggg cctatttccc atgattcctt catatttgca tatacgatac
     6121 aaggctgtta gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa
     6181 aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt
     6241 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat
     6301 atcttgtgga aaggacgaaa caccggacct accggctccg cggttttaga gctagaaata
     6361 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
     6421 tttttaccgt cgacctctag ctagagcttg gcgtaatcga gcttggcgta atcatggtac
     6481 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     6541 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     6601 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     6661 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     6721 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     6781 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     6841 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     6901 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     6961 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     7021 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7081 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     7141 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     7201 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     7261 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     7321 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     7381 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     7441 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     7501 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     7561 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     7621 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     7681 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     7741 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     7801 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     7861 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     7921 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     7981 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     8041 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     8101 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     8161 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     8221 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     8281 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     8341 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     8401 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     8461 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     8521 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     8581 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     8641 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     8701 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     8761 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     8821 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     8881 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     8941 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     9001 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     9061 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     9121 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     9181 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     9241 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     9301 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     9361 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     9421 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     9481 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     9541 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     9601 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     9661 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     9721 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     9781 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     9841 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     9901 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     9961 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
    10021 aagctt
//
