LOCUS       Exported               11091 bp ds-DNA     circular SYN 28-MAY-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11091)
  AUTHORS   Ryan Lister
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Oct 31, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11091
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     170..227
                     /label=OL-578
     primer_bind     complement(170..227)
                     /label=OL-585
     promoter        209..712
                     /label=pPGK
     primer_bind     645..666
                     /label=OL-D152
     primer_bind     688..743
                     /label=OL 652
     primer_bind     complement(688..743)
                     /label=OL 653
     primer_bind     721..750
                     /label=OL-D62
     primer_bind     741..769
                     /label=OC-599_NLS_SSSavi_catcher_pA_gateway_P1_F
     misc_feature    744..764
                     /label=Amino acid translation
     CDS             744..764
                     /codon_start=1
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             765..854
                     /codon_start=1
                     /label=3xHA
                     /translation="YPYDVPDYAAYPYDVPDYAAYPYDVPDYAA"
     misc_feature    855..860
                     /label=Amino acid translation
     misc_feature    861..5000
                     /label=Amino acid translation
     primer_bind     complement(866..884)
                     /label=OL-D211
     CDS             885..5000
                     /codon_start=1
                     /label=dCas9
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGDSRAD"
     variation       912..914
                     /label=D10A
     primer_bind     complement(1362..1397)
                     /label=OL 655
     primer_bind     complement(2607..2636)
                     /label=OL-D245_dCas9_R
     primer_bind     2607..2636
                     /label=OL-D244_dCas9_F
     variation       3402..3404
                     /label=H840A
     misc_feature    5001..5126
                     /label=Amino acid translation
     CDS             5001..5126
                     /codon_start=1
                     /label=Linker V3
                     /translation="GGGSGGGSPKKKRKVTGKKTSGQAGQASPKKKRKVGRAGGGS"
     sig_peptide     5025..5045
                     /label=SV40 NLS
     sig_peptide     5085..5105
                     /label=SV40 NLS
     primer_bind     complement(5108..5165)
                     /label=TOL766_dCas9-SpyTag_R
     CDS             5127..5165
                     /codon_start=1
                     /label=SpyTag
                     /translation="AHIVMVDAYKPTK"
     primer_bind     5177..5195
                     /label=TOL788_aGCN4x5_F
     CDS             5232..5270
                     /codon_start=1
                     /label=SnoopTag
                     /translation="GKLGDIEFIKVNK"
     CDS             5337..5393
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     primer_bind     5388..5426
                     /label=TOL788_aGCN4x5_F
     CDS             5463..5519
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5586..5642
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5709..5765
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5832..5888
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     primer_bind     complement(5874..5921)
                     /label=TOL789_aGCN4x5_R
     primer_bind     5949..6008
                     /label=TOL765_dCas9-TraptAviTag_F
     CDS             5949..5993
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     misc_feature    6018..6038
                     /label=SV40 NLS
     misc_feature    6045..6314
                     /label=HSV TK polyA
     primer_bind     6130..6151
                     /label=OL - D153
     primer_bind     complement(6297..6317)
                     /label=OC-603_NLS_SSSavi_catcher_pA_Gib_Tm60_R
     primer_bind     complement(6297..6314)
                     /label=OC-600_NLS_SSSavi_catcher_pA_gateway_P5r_R
     promoter        6315..7572
                     /label=EF1a
     primer_bind     6755..6776
                     /label=OL -D154
     primer_bind     7367..7388
                     /label=OL- D155
     primer_bind     complement(7563..7587)
                     /label=OL-581
     CDS             7585..8295
                     /codon_start=1
                     /label=Tag-BFP2
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     misc_feature    8299..8596
                     /label=IVS
     primer_bind     8577..8636
                     /label=OL-584
     polyA_signal    8604..8842
                     /label=SV40 polyA
     rep_origin      9321..9940
                     /label=pBR322 Orgin
     primer_bind     complement(9985..10023)
                     /label=TOL768_dCas9_R
     primer_bind     9985..10023
                     /label=TOL767_dCas9_F
     CDS             complement(10095..10955)
                     /codon_start=1
                     /label=Ampicillin
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10997..11025)
                     /label=AMP promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ttaattaata ccgggtaggg gaggcgcttt tcccaaggca
      241 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
      301 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
      361 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
      421 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
      481 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
      541 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
      601 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
      661 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cggcggccgc
      721 acctgcagcc caagcttacc atgccaaaga agaagcggaa ggtctacccc tatgacgtgc
      781 ctgattacgc cgcttatcct tacgatgtgc ccgactatgc agcctaccca tacgatgtgc
      841 ccgactacgc cgcaatggcc ggtatccacg gagtcccagc agccatggac aagaagtact
      901 ccattgggct cgctatcggc acaaacagcg tcggctgggc cgtcattacg gacgagtaca
      961 aggtgccgag caaaaaattc aaagttctgg gcaataccga tcgccacagc ataaagaaga
     1021 acctcattgg cgccctcctg ttcgactccg gggagacggc cgaagccacg cggctcaaaa
     1081 gaacagcacg gcgcagatat acccgcagaa agaatcggat ctgctacctg caggagatct
     1141 ttagtaatga gatggctaag gtggatgact ctttcttcca taggctggag gagtcctttt
     1201 tggtggagga ggataaaaag cacgagcgcc acccaatctt tggcaatatc gtggacgagg
     1261 tggcgtacca tgaaaagtac ccaaccatat atcatctgag gaagaagctt gtagacagta
     1321 ctgataaggc tgacttgcgg ttgatctatc tcgcgctggc gcatatgatc aaatttcggg
     1381 gacacttcct catcgagggg gacctgaacc cagacaacag cgatgtcgac aaactcttta
     1441 tccaactggt tcagacttac aatcagcttt tcgaagagaa cccgatcaac gcatccggag
     1501 ttgacgccaa agcaatcctg agcgctaggc tgtccaaatc ccggcggctc gaaaacctca
     1561 tcgcacagct ccctggggag aagaagaacg gcctgtttgg taatcttatc gccctgtcac
     1621 tcgggctgac ccccaacttt aaatctaact tcgacctggc cgaagatgcc aagcttcaac
     1681 tgagcaaaga cacctacgat gatgatctcg acaatctgct ggcccagatc ggcgaccagt
     1741 acgcagacct ttttttggcg gcaaagaacc tgtcagacgc cattctgctg agtgatattc
     1801 tgcgagtgaa cacggagatc accaaagctc cgctgagcgc tagtatgatc aagcgctatg
     1861 atgagcacca ccaagacttg actttgctga aggcccttgt cagacagcaa ctgcctgaga
     1921 agtacaagga aattttcttc gatcagtcta aaaatggcta cgccggatac attgacggcg
     1981 gagcaagcca ggaggaattt tacaaattta ttaagcccat cttggaaaaa atggacggca
     2041 ccgaggagct gctggtaaag cttaacagag aagatctgtt gcgcaaacag cgcactttcg
     2101 acaatggaag catcccccac cagattcacc tgggcgaact gcacgctatc ctcaggcggc
     2161 aagaggattt ctaccccttt ttgaaagata acagggaaaa gattgagaaa atcctcacat
     2221 ttcggatacc ctactatgtg ggccccctcg cccggggaaa ttccagattc gcgtggatga
     2281 ctcgcaaatc agaagagacc atcactccct ggaacttcga ggaagtcgtg gataaggggg
     2341 cctctgccca gtccttcatc gaaaggatga ctaactttga taaaaatctg cctaacgaaa
     2401 aggtgcttcc taaacactct ctgctgtacg agtacttcac agtttataac gagctcacca
     2461 aggtcaaata cgtcacagaa gggatgagaa agccagcatt cctgtctgga gagcagaaga
     2521 aagctatcgt ggacctcctc ttcaagacga accggaaagt taccgtgaaa cagctcaaag
     2581 aagactattt caaaaagatt gaatgtttcg actctgttga aatcagcgga gtggaggatc
     2641 gcttcaacgc atccctggga acgtatcacg atctcctgaa aatcattaaa gacaaggact
     2701 tcctggacaa tgaggagaac gaggacattc ttgaggacat tgtcctcacc cttacgttgt
     2761 ttgaagatag ggagatgatt gaagaacgct tgaaaactta cgctcatctc ttcgacgaca
     2821 aagtcatgaa acagctcaag aggcgccgat atacaggatg ggggcggctg tcaagaaaac
     2881 tgatcaatgg gatccgagac aagcagagtg gaaagacaat cctggatttt cttaagtccg
     2941 atggatttgc caacaggaac ttcatgcagt tgatccatga tgactctctc acctttaagg
     3001 aggacatcca gaaagcacaa gtttctggcc agggggacag tcttcacgag cacatcgcta
     3061 atcttgcagg tagcccagct atcaaaaagg gaatactgca gaccgttaag gtcgtggatg
     3121 aactcgtcaa agtaatggga aggcataagc ccgagaatat cgttatcgag atggcccgag
     3181 agaaccaaac tacccagaag ggacagaaga acagtaggga aaggatgaag aggattgaag
     3241 agggtataaa agaactgggg tcccaaatcc ttaaggaaca cccagttgaa aacacccagc
     3301 ttcagaatga gaagctctac ctgtactacc tgcagaacgg cagggacatg tacgtggatc
     3361 aggaactgga catcaatcgg ctctccgact acgacgtgga tgcaatcgtg ccccagtctt
     3421 ttctcaaaga tgattctatt gataataaag tgttgacaag atccgataaa aatagaggga
     3481 agagtgataa cgtcccctca gaagaagttg tcaagaaaat gaaaaattat tggcggcagc
     3541 tgctgaacgc caaactgatc acacaacgga agttcgataa tctgactaag gctgaacgag
     3601 gtggcctgtc tgagttggat aaagccggct tcatcaaaag gcagcttgtt gagacacgcc
     3661 agatcaccaa gcacgtggcc caaattctcg attcacgcat gaacaccaag tacgatgaaa
     3721 atgacaaact gattcgagag gtgaaagtta ttactctgaa gtctaagctg gtctcagatt
     3781 tcagaaagga ctttcagttt tataaggtga gagagatcaa caattaccac catgcgcatg
     3841 atgcctacct gaatgcagtg gtaggcactg cacttatcaa aaaatatccc aagcttgaat
     3901 ctgaatttgt ttacggagac tataaagtgt acgatgttag gaaaatgatc gcaaagtctg
     3961 agcaggaaat aggcaaggcc accgctaagt acttctttta cagcaatatt atgaattttt
     4021 tcaagaccga gattacactg gccaatggag agattcggaa gcgaccactt atcgaaacaa
     4081 acggagaaac aggagaaatc gtgtgggaca agggtaggga tttcgcgaca gtccggaagg
     4141 tcctgtccat gccgcaggtg aacatcgtta aaaagaccga agtacagacc ggaggcttct
     4201 ccaaggaaag tatcctcccg aaaaggaaca gcgacaagct gatcgcacgc aaaaaagatt
     4261 gggaccccaa gaaatacggc ggattcgatt ctcctacagt cgcttacagt gtactggttg
     4321 tggccaaagt ggagaaaggg aagtctaaaa aactcaaaag cgtcaaggaa ctgctgggca
     4381 tcacaatcat ggagcgatca agcttcgaaa aaaaccccat cgactttctc gaggcgaaag
     4441 gatataaaga ggtcaaaaaa gacctcatca ttaagcttcc caagtactct ctctttgagc
     4501 ttgaaaacgg ccggaaacga atgctcgcta gtgcgggcga gctgcagaaa ggtaacgagc
     4561 tggcactgcc ctctaaatac gttaatttct tgtatctggc cagccactat gaaaagctca
     4621 aagggtctcc cgaagataat gagcagaagc agctgttcgt ggaacaacac aaacactacc
     4681 ttgatgagat catcgagcaa ataagcgaat tctccaaaag agtgatcctc gccgacgcta
     4741 acctcgataa ggtgctttct gcttacaata agcacaggga taagcccatc agggagcagg
     4801 cagaaaacat tatccacttg tttactctga ccaacttggg cgcgcctgca gccttcaagt
     4861 acttcgacac caccatagac agaaagcggt acacctctac aaaggaggtc ctggacgcca
     4921 cactgattca tcagtcaatt acggggctct atgaaacaag aatcgacctc tctcagctcg
     4981 gtggagacag cagggctgac gggggaggat cagggggagg gtcccccaaa aagaaacgca
     5041 aagtgaccgg caagaaaacc agcggccagg ccggacaggc ctctcctaag aaaaaaagaa
     5101 aagtgggacg cgctggcggc ggaagcgccc acatcgtgat ggtggacgcc tacaagccga
     5161 cgaagggaag cgggtccggt ggaagtggct ccggatctgg aggttctggc agcggaggta
     5221 gcggcagtgg cgggaaactg ggcgatattg aatttattaa agtgaacaaa gggtcgggaa
     5281 gtggcggcag cggaagtggg agtggaggga gcggttctgg cggttccggc agtggagagg
     5341 agcttctgag caaaaactat cacctcgaaa acgaggttgc gcgactgaag aaaggttccg
     5401 gctccaacgg tggaagtggc tccggatctg gaggttctgg cagcggaggt agcggcagtg
     5461 gcgaagagct ccttagtaag aactatcatc tggaaaatga ggtagcgcgc ttaaagaaag
     5521 ggtcgggaag tggcggcagc ggaagtggga gtggagggag cggttctggc ggttccggca
     5581 gtggagagga gttgctgtct aagaactacc acttagaaaa cgaagtcgca cggctaaaaa
     5641 aaggttccgg ctccggcggc tccggttctg gaagcggggg ctcgggatca ggtggatctg
     5701 gatcaggaga ggaattgctt tccaaaaact accaccttga gaatgaggtg gccaggttaa
     5761 agaaggggag cggctcgggg ggtagtggat cggggtcggg cgggtcagga agcggtggta
     5821 gcggatctgg ggaggagctg ctctcgaaga attaccattt ggagaacgaa gtggcgagac
     5881 taaagaaggg aagcggtagt tccggttctg gaagcggggg ctcgggatca ggtggatctg
     5941 gatcaggagg cctgaacgat atttttgaag cgcagaaaat tgaatggcat gaaggcggtg
     6001 gttccggagg aggtagtccg aagaagaaac gtaaggtcgg atgaggggga ggctaactga
     6061 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
     6121 taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
     6181 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
     6241 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
     6301 aggccctgcc atagtgcaaa gatggataaa gttttaaaca gagaggaatc tttgcagcta
     6361 atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc agtgggcaga
     6421 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc
     6481 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
     6541 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
     6601 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct
     6661 ctttacgggt tatggccctt gcgtgccttg aattacttcc actggctgca gtacgtgatt
     6721 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga
     6781 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga
     6841 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat
     6901 ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa
     6961 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc
     7021 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
     7081 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
     7141 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
     7201 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
     7261 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
     7321 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
     7381 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     7441 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     7501 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     7561 caggtgtcgt gacgtacggc caccatggtg tctaagggcg aagagctgat taaggagaac
     7621 atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa gtgcacatcc
     7681 gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc
     7741 ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg cagcaagacc
     7801 ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc tgagggcttc
     7861 acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc
     7921 agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa cttcacatcc
     7981 aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga gacgctgtac
     8041 cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt gggcgggagc
     8101 catctgatcg caaacgccaa gaccacatat agatccaaga aacccgctaa gaacctcaag
     8161 atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc caacaacgag
     8221 acctacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc tagcaaactg
     8281 gggcacaagc ttaattgatg gaattaattc gctgtctgcg agggccagct gttggggtga
     8341 gtactccctc tcaaaagcgg gcatgacttc tgcgctaaga ttgtcagttt ccaaaaacga
     8401 ggaggatttg atattcacct ggcccgcggt gatgcctttg agggtggccg cgtccatctg
     8461 gtcagaaaag acaatctttt tgttgtcaag cttgaggtgt ggcaggcttg agatctggcc
     8521 atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt ccactcccag
     8581 gtccaactgc aggtcgagat aactgatcat aatcagccat accacatttg tagaggtttt
     8641 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     8701 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     8761 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     8821 caatgtatct taatgcgtcg agtgcattct agttgtggtt tgtccaaact catcaatgta
     8881 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     8941 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     9001 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     9061 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     9121 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     9181 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     9241 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     9301 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     9361 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     9421 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     9481 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     9541 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     9601 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     9661 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     9721 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     9781 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     9841 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     9901 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     9961 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    10021 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    10081 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    10141 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    10201 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    10261 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    10321 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    10381 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    10441 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    10501 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    10561 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    10621 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    10681 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    10741 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    10801 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    10861 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    10921 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    10981 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    11041 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
