LOCUS       895_pDonorTp-fugfp__col9_                 3875 bp    DNA     circular SYN 14-JUL-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             2603..2839
                     /note="pLannotate"
                     /label="TpR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     terminator      complement(410..504)
                     /note="pLannotate"
                     /label="lambda t0 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     terminator      complement(221..307)
                     /note="pLannotate"
                     /label="rrnB T1 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     CDS             3144..3860
                     /note="pLannotate"
                     /label="EBFP1.2"
                     /database="fpbase"
                     /identity="80.3"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     protein_bind    complement(1806..1853)
                     /note="pLannotate"
                     /label="FRT"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     rep_origin      complement(848..1223)
                     /note="pLannotate"
                     /label="R6K γ ori"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="96.7"
                     /fragment="False"
                     /other="rep_origin"
     promoter        3085..3119
                     /note="pLannotate"
                     /label="J23119(SpeI) promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(3011..3044)
                     /note="pLannotate"
                     /label="FRT (minimal)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        2249..2277
                     /note="pLannotate"
                     /label="Pc promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     oriT            complement(1255..1363)
                     /note="pLannotate"
                     /label="oriT"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="99.1"
                     /fragment="False"
                     /other="oriT"
     CDS             1912..2208
                     /note="pLannotate"
                     /label="tnpA (fragment)"
                     /database="swissprot"
                     /identity="99.0"
                     /match_length="20.8"
                     /fragment="True"
                     /other="CDS"
     CDS             70..199
                     /note="pLannotate"
                     /label="KanR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="16.0"
                     /fragment="True"
                     /other="CDS"
     ncRNA           2453..2519
                     /note="pLannotate"
                     /label="AAC AAD leader (fragment)"
                     /database="Rfam"
                     /identity="100.0"
                     /match_length="89.3"
                     /fragment="True"
                     /other="ncRNA"
     misc_feature    complement(775..798)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="17.0"
                     /fragment="True"
                     /other="misc_feature"
     rep_origin      799..842
                     /note="pLannotate"
                     /label="ori (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="7.5"
                     /fragment="True"
                     /other="rep_origin"
     CDS             complement(371..400)
                     /note="pLannotate"
                     /label="CmR (fragment)"
                     /database="snapgene"
                     /identity="96.7"
                     /match_length="4.4"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 aaaaaaaaac cccgcccctg acagggcggg gtttttttgg taccgtcata gctgtttccc
       61 atatgtaatt ccccggcaaa acagcattcc aggtattaga agaatatcct gattcaggtg
      121 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta
      181 attgtccttt taacagcgaa ttacatatgc ctagcggcgg atttgtccta ctcaggagag
      241 cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt
      301 ttatttgatg cctcaagcta gagagtcatt accccaggcg tttaagggca ccaataactg
      361 ccttaaaaaa attacgcccc gccctgccac tcatcgcagt ctagcttgga ttctcaccaa
      421 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
      481 ttactggatc tatcaacagg agtccaagct cagctaatta aggcgacagt caatttgtca
      541 ttatgaaaat acacaaaagc tttttcctat cttgcaaagc gacagctaat ttgtcacaat
      601 cacggacaac gacatctatt ttgtcactgc aaagaggtta tgctaaaact gccaaagcgc
      661 tataatctat actgtataag gattttactg atgacaataa tttgtcacaa cgacatataa
      721 ttagtcactg tacacgtaga gacgtagcaa tgctacctct ctacaatggt tttgtatggt
      781 gcactctcag tacaatcttg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      841 agccggttgt cagccgttaa gtgttcctgt gtcactcaaa attgctttga gaggctctaa
      901 gggcttctca gtgcgttaca tccctggctt gttgtccaca accgttaaac cttaaaagct
      961 ttaaaagcct tatatattct tttttttctt ataaaactta aaaccttaga ggctatttaa
     1021 gttgctgatt tatattaatt ttattgttca aacatgagag cttagtacgt gaaacatgag
     1081 agcttagtac gttagccatg agagcttagt acgttagcca tgagggttta gttcgttaaa
     1141 catgagagct tagtacgtta aacttgagag cttagtacgt gaaacatgag agcttagtac
     1201 gtactatcaa caggttgaac tgcccatgtt ctttcctgcg ttatcagagc ttatcggcca
     1261 gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc gaataaggga cagtgaagaa
     1321 ggaacacccg ctcgcgggtg ggcctacttc acctatcctg cccggctgac gccgttggat
     1381 acaccaagga aagtctacac gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga
     1441 tggatatacc gaaaaaatcg ctataatgac cccgaagcag ggttatgcag cggaaagtaa
     1501 aaaattttta gtttattaga catctccaca aaaggcgtag tgtacagtga caaattatct
     1561 gtcgtcggtg acagattaat gtcattgtga ctatttaatt gtcgtcgtga cccatcagcg
     1621 ttgcttaatt aattgatgac aaattaaatg tcatcaatat aatatgctct gcaattatta
     1681 tacaaagcaa ttaaaacaag cggataaaag gacttgcttt caacccaccc ctaagtttaa
     1741 tagttactga gggggatcca ctagtgagct catgcatgat ctcgaattag cttcaaaagc
     1801 gctctgaagt tcctatactt tctagagaat aggaacttcg gaataggaac ttcaagatcc
     1861 cctgattccc tttgtcaaca gcaatggata attcgattta acaaatgcat ggcgcaaggg
     1921 ctgctaaagg aagcggaaca cgtagaaagc cagtccgcag aaacggtgct gaccccggat
     1981 gaatgtcagc tactgggcta tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt
     2041 agcttgcagt gggcttacat ggcgatagct agactgggcg gttttatgga cagcaagcga
     2101 accggaattg ccagctgggg cgccctctgg taaggttggg aagccctgca aagtaaactg
     2161 gatggctttc ttgccgccaa ggatctgatg gcgcagggga tcaagatctg atcaagagac
     2221 aggatgaggg gtacccgcac gaacccagtt gacataagcc tgttcggttc gtaaactgta
     2281 atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
     2341 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgtac agtctatgcc
     2401 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     2461 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaggcagcc gttgtgctgg
     2521 tgctttctga tagttgttgt ggggtaggca gtcagagctc gatttgcttg tcgccataat
     2581 agattcacaa gaaggattcg acatgggtca aagtagcgat gaagccaacg ctcccgttgc
     2641 agggcagttt gcgcttcccc tgagtgccac ctttggctta ggggatcgcg tacgcaagaa
     2701 atctggtgcc gcttggcagg gtcaagtcgt cggttggtat tgcacaaaac tcactcctga
     2761 aggctatgcg gtcgagtccg aatcccaccc aggctcagtg caaatttatc ctgtggctgc
     2821 acttgaacgt gtggcctaag cgagctctta attaatttaa atctagacta gtgcggccgc
     2881 acttgtgtat aagagtcaga attctactcg agggaagagt atgaggatcc aacatttcca
     2941 atcactagtg aattatctag aattattcca ttgagtaagt ttttaagcac atcagcttca
     3001 aaagcgctct gaagttccta tactttctag agaataggaa cttcggaata ggtacttcaa
     3061 gatccccaat tcgagatccc cgggttgaca gctagctcag tcctaggtat aatactagta
     3121 gattaaagag gagaaagagt cccatggtga gcagtggcga agatattttc tcgggcttgg
     3181 ttccgattct gatcgagctg gagggcgacg tgaacggtca tcgttttagc gttcgcggtg
     3241 aaggttatgg cgacgcgagc aacggcaaac tggaaattaa gttcatctgc acgaccggtc
     3301 gcctgccggt gccttggccg accttggtga cgaccttgtc gtatggcgtg cagtgttttg
     3361 cgaagtatcc ggagcacatg cgccaaaacg atttctttaa aagtgccatg ccggacggtt
     3421 acgtccagga gcgtaccatt tccttcaagg aagatggcac gtacaaaact cgcgcagagg
     3481 ttaagtttga aggtgaagcg ctggtcaatc gtatcgattt gaagggtttg gagtttaaag
     3541 aggatggtaa cattctgggc cataaactgg agtatagctt caacagccat tatgtttaca
     3601 ttacggcaga caagaatcgt aacggcttgg aggcccaatt ccgtattcgc cacaatgttg
     3661 atgacggtag cgtccaactg gccgaccatt accaacagaa caccccaatt ggtgagggtc
     3721 cggtgttgct gccggaacaa cactatctga cgaccaatag cgtgctgagc aaagatccgc
     3781 aggaacgccg tgatcacatg gtcctggtgg aatttgtgac cgctgcgggc ttgagcctgg
     3841 gtatggacga gctgtataag agctaataag agctc
//
