LOCUS       Exported                9275 bp ds-DNA     circular SYN 03-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9275)
  AUTHORS   L Huang, M Rojas-Pierce, NC State U
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 3, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9275
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    17..20
                     /label=GGAG
     promoter        21..1617
                     /label=pUBQ10
                     /note="derived from UBQ10"
     exon            1618..1702
                     /number=1.1
                     /label=Exon_1.1
     intron          1703..2006
                     /number=1.1
                     /label=Intron_1.1
     misc_feature    2007..2010
                     /label=AATG
     misc_feature    2008..2727
                     /label=GFP
     misc_feature    2728..2731
                     /label=GCTT
     terminator      2732..2920
                     /label=35S terminator
     misc_feature    2921..2924
                     /label=CGCT
     misc_feature    2925..2928
                     /label=GTCA
     misc_feature    2941..2965
                     /label=RB
     misc_feature    complement(4027..5027)
                     /label=STA region from pVS1 plasmid
                     /label=STA\region\from\pVS1\plasmid
     misc_feature    complement(5620..6620)
                     /label=pVS1-Rep ori
                     /label=pVS1-Rep\ori
     misc_feature    complement(7030..7290)
                     /label=pBR322
     rep_origin      complement(7430..7710)
                     /direction=LEFT
                     /label=pBR322 ori
                     /label=pBR322\ori
     CDS             complement(8005..8817)
                     /label=KanR
     misc_feature    9250..9275
                     /label=LB
ORIGIN
        1 taacgaattc gtctcaggag agtctagctc aacagagctt ttaacccaaa ttggtacaat
       61 agaatacaac tttagatcat aattctcaaa agaaagagat tccttagcta ttctatctgc
      121 cactccattt ccttctcggc ttgtatgcac aagcataaaa tcctcaaact tgctaagtag
      181 atactttatg tcttggataa ttggattgag acttgacaag cataactttc atgtaaccaa
      241 agacacaagt tgctgagaat ccacctcaaa aatgatcttc ctataattga atcgggataa
      301 tgacagcaca gcccatctaa gagcctccac ttctacttcc agcacgcttc ttacttttac
      361 cacagctctt gcacctaacc ataacacctt ccctgtatga tcgcgaagca cccaccctaa
      421 gccacatttt aatccttctg ttggccatgc cccatcaaag ttgcacttaa cccaagattg
      481 tggtggagct tcccatgttt ctcgtctgtc ccgacggtgt tgtggttggt gctttcctta
      541 cattctgagc ctctttcctt ctaatccact catctgcatc ttcttgtgtc cttactaata
      601 cctcattggt tccaaattcc ctccctttaa gcaccagctc gtttctgttc ttccacagcc
      661 tcccaagtat ccaagggact aaagcctcca cattcttcag atcaggatat tcttgtttaa
      721 gatgttgaac tctatggagg tttgtatgaa ctgatgatct aggaccggat aagttccctt
      781 cttcatagcg aacttattca aagaatgttt tgtgtatcat tcttgttaca ttgttattaa
      841 tgaaaaaata ttattggtca ttggactgaa cacgagtgtt aaatatggac caggccccaa
      901 ataagatcca ttgatatatg aattaaataa caagaataaa tcgagtcacc aaaccacttg
      961 ccttttttaa cgagacttgt tcaccaactt gatacaaaag tcattatcct atgcaaatca
     1021 ataatcatac aaaaatatcc aataacacta aaaaattaaa agaaatggat aatttcacaa
     1081 tatgttatac gataaagaag ttacttttcc aagaaattca ctgattttat aagcccactt
     1141 gcattagata aatggcaaaa aaaaacaaaa aggaaaagaa ataaagcacg aagaattcta
     1201 gaaaatacga aatacgcttc aatgcagtgg gacccacggt tcaattattg ccaattttca
     1261 gctccaccgt atatttaaaa aataaaacga taatgctaaa aaaatataaa tcgtaacgat
     1321 cgttaaatct caacggctgg atcttatgac gaccgttaga aattgtggtt gtcgacgagt
     1381 cagtaataaa cggcgtcaaa gtggttgcag ccggcacaca cgagtcgtgt ttatcaactc
     1441 aaagcacaaa tacttttcct caacctaaaa ataaggcaat tagccaaaaa caactttgcg
     1501 tgtaaacaac gctcaataca cgtgtcattt tattattagc tattgcttca ccgccttagc
     1561 tttctcgtga cctagtcgtc ctcgtctttt cttcttcttc ttctataaaa caatacccaa
     1621 agagctcttc ttcttcacaa ttcagatttc aatttctcaa aatcttaaaa actttctctc
     1681 aattctctct accgtgatca aggtaaattt ctgtgttcct tattctctca aaatcttcga
     1741 ttttgttttc gttcgatccc aatttcgtat atgttctttg gtttagattc tgttaatctt
     1801 agatcgaaga cgattttctg ggtttgatcg ttagatatca tcttaattct cgattagggt
     1861 ttcatagata tcatccgatt tgttcaaata atttgagttt tgtcgaataa ttactcttcg
     1921 atttgtgatt tctatctaga tctggtgtta gtttctagtt tgtgcgatcg aatttgtcga
     1981 ttaatctgag tttttctgat taacagaatg gtgagcaagg gcgaggagct gttcaccggg
     2041 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     2101 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     2161 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     2221 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     2281 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     2341 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     2401 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     2461 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     2521 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     2581 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     2641 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     2701 ctcggcatgg acgagctgta caagtaagct taaatcacca gtctctctct acaaatctat
     2761 ctctctctat aataatgtgt gagtagttcc cagataaggg aattagggtt cttatagggt
     2821 ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact
     2881 tctatcaata aaatttctaa ttcctaaaac caaaatccag cgctgtcatg agacgaattc
     2941 tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat
     3001 atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca
     3061 gggttcccct cgggatcaaa gtactttgat ccaacccctc cgctgctata gtgcagtcgg
     3121 cttctgacgt tcagtgcagc cgtcatctga aaacgacatg tcgcacaagt cctaagttac
     3181 gcgacaggct gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat
     3241 aaagtagaat acttgcgact agaaccggag acattacgcc atgaacaaga gcgccgccgc
     3301 tggcctgctg ggctatgccc gcgtcagcac cgacgaccag gacttgacca accaacgggc
     3361 cgaactgcac gcggccggct gcaccaagct gttttccgag aagatcaccg gcaccaggcg
     3421 cgaccgcccg gagctggcca ggatgcttga ccacctacgc cctggcgacg ttgtgacagt
     3481 gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta ctggacattg ccgagcgcat
     3541 ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc
     3601 ggccggccgc atggtgttga ccgtgttcgc cggcattgcc gagttcgagc gttccctaat
     3661 catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc
     3721 ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg accaggaagg
     3781 ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc
     3841 acttgagcgc agcgaggaag tgacgcccac cgaggccagg cggcgcggtg ccttccgtga
     3901 ggacgcattg accgaggccg acgccctggc ggccgccgag aatgaacgcc aagaggaaca
     3961 agcatgaaac cgcaccagga cggccaggac gaaccgtttt tcattaccga agagatcgag
     4021 gcggagatga tcgcggccgg gtacgtgttc gagccgcccg cgcacctctc aaccgtgcgg
     4081 ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg cggcctggcc ggccagcttg
     4141 gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc
     4201 ttgcgtcatg cggtcgctgc gtatatgatc cgatgagtaa ataaacaaat acgcaagggg
     4261 aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg
     4321 gaacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta gtcgattccg
     4381 atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg
     4441 tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg cgcgacttcg
     4501 tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc aaggcagccg
     4561 acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc gccgacctgg
     4621 tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg gcctttgtcg
     4681 tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt
     4741 acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca ggcactgccg
     4801 ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc gaggtccagg
     4861 cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag agaaaatgag
     4921 caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca aggctgcaac
     4981 gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt tgccggcgga
     5041 ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta ccgagctgct
     5101 atctgaatag atcgcgcagc taccagagta aatgagcaaa tgaataaatg agtagatgaa
     5161 ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc gacgccgtgg
     5221 aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc
     5281 ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgacgg tcgcaaacca
     5341 tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc
     5401 gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa
     5461 gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg
     5521 attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac
     5581 gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt
     5641 gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc
     5701 gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat ggcggtttcc
     5761 catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg
     5821 ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag
     5881 aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt
     5941 acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc
     6001 cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct
     6061 gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac ggttcacccc
     6121 gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc
     6181 gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc
     6241 ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg
     6301 gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc
     6361 aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa
     6421 attgccctag caggggaaaa aggtcgaaaa ggactctttc ctgtggatag cacgtacatt
     6481 gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac
     6541 attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc
     6601 gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg
     6661 tctggccagc gcacagccga agagctgcaa aaagcgccta cccttcggtc gctgcgctcc
     6721 ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa aatggctggc
     6781 ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact cgaccgccgg
     6841 cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     6901 catgcagctc ccggtgacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     6961 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
     7021 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga
     7081 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     7141 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7201 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7261 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7321 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7381 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7441 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7501 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     7561 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     7621 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     7681 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     7741 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     7801 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     7861 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     7921 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     7981 ttggtcatgc attctaggtg attagaaaaa ctcatcgagc atcaaatgaa actgcaattt
     8041 attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga
     8101 aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac
     8161 tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga
     8221 gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagtttat gcatttcttt
     8281 ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa
     8341 accgttattc attcgtgatt gcgcctgagc gagtcgaaat acgcgatcgc tgttaaaagg
     8401 acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat
     8461 attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc ctgggatcgc
     8521 agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg
     8581 cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct
     8641 acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcggtagat
     8701 tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc
     8761 catgttggaa tttaatcgcg gccttgagca agacgtttcc cgttgaatat ggctcataac
     8821 agaacttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     8881 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     8941 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     9001 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     9061 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     9121 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     9181 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     9241 gctggctggt ggcaggatat attgtggtgt aaaca
//
