LOCUS       Exported                9518 bp ds-DNA     circular SYN 03-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9518)
  AUTHORS   L Huang, M Rojas-Pierce, NC State U
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 3, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9518
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    17..20
                     /label=GGAG
     promoter        21..1617
                     /label=pUBQ10
                     /note="derived from UBQ10"
     exon            1618..1702
                     /number=1.1
                     /label=Exon_1.1
     intron          1703..2006
                     /number=1.1
                     /label=Intron_1.1
     misc_feature    2007..2010
                     /label=AATG
     CDS             2011..2243
                     /codon_start=1
                     /label=HR1b
                     /note="HR1b aa122-199 from Kezei et al"
                     /translation="ATNLSRVAGLEKQLAIELKVKQGAENMIQTYSNGSTKDRKLLLTA
                     QQMLQDSKTKIDIIAMQLARALQAGQLENQAA"
     misc_feature    2188..2190
                     /label=R181-A181
     misc_feature    2200..2202
                     /label=R185-A185
     CDS             2251..2970
                     /codon_start=1
                     /label=GFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      2975..3163
                     /label=35S terminator
     misc_feature    3164..3167
                     /label=CGCT
     misc_feature    3168..3171
                     /label=GTCA
     misc_feature    3184..3208
                     /label=RB
     misc_feature    complement(4270..5270)
                     /label=STA region from pVS1 plasmid
                     /label=STA\region\from\pVS1\plasmid
     misc_feature    complement(5863..6863)
                     /label=pVS1-Rep ori
                     /label=pVS1-Rep\ori
     misc_feature    complement(7273..7533)
                     /label=pBR322
     rep_origin      complement(7673..7953)
                     /direction=LEFT
                     /label=pBR322 ori
                     /label=pBR322\ori
     CDS             complement(8248..9060)
                     /label=KanR
     misc_feature    9493..9518
                     /label=LB
ORIGIN
        1 taacgaattc gtctcaggag agtctagctc aacagagctt ttaacccaaa ttggtacaat
       61 agaatacaac tttagatcat aattctcaaa agaaagagat tccttagcta ttctatctgc
      121 cactccattt ccttctcggc ttgtatgcac aagcataaaa tcctcaaact tgctaagtag
      181 atactttatg tcttggataa ttggattgag acttgacaag cataactttc atgtaaccaa
      241 agacacaagt tgctgagaat ccacctcaaa aatgatcttc ctataattga atcgggataa
      301 tgacagcaca gcccatctaa gagcctccac ttctacttcc agcacgcttc ttacttttac
      361 cacagctctt gcacctaacc ataacacctt ccctgtatga tcgcgaagca cccaccctaa
      421 gccacatttt aatccttctg ttggccatgc cccatcaaag ttgcacttaa cccaagattg
      481 tggtggagct tcccatgttt ctcgtctgtc ccgacggtgt tgtggttggt gctttcctta
      541 cattctgagc ctctttcctt ctaatccact catctgcatc ttcttgtgtc cttactaata
      601 cctcattggt tccaaattcc ctccctttaa gcaccagctc gtttctgttc ttccacagcc
      661 tcccaagtat ccaagggact aaagcctcca cattcttcag atcaggatat tcttgtttaa
      721 gatgttgaac tctatggagg tttgtatgaa ctgatgatct aggaccggat aagttccctt
      781 cttcatagcg aacttattca aagaatgttt tgtgtatcat tcttgttaca ttgttattaa
      841 tgaaaaaata ttattggtca ttggactgaa cacgagtgtt aaatatggac caggccccaa
      901 ataagatcca ttgatatatg aattaaataa caagaataaa tcgagtcacc aaaccacttg
      961 ccttttttaa cgagacttgt tcaccaactt gatacaaaag tcattatcct atgcaaatca
     1021 ataatcatac aaaaatatcc aataacacta aaaaattaaa agaaatggat aatttcacaa
     1081 tatgttatac gataaagaag ttacttttcc aagaaattca ctgattttat aagcccactt
     1141 gcattagata aatggcaaaa aaaaacaaaa aggaaaagaa ataaagcacg aagaattcta
     1201 gaaaatacga aatacgcttc aatgcagtgg gacccacggt tcaattattg ccaattttca
     1261 gctccaccgt atatttaaaa aataaaacga taatgctaaa aaaatataaa tcgtaacgat
     1321 cgttaaatct caacggctgg atcttatgac gaccgttaga aattgtggtt gtcgacgagt
     1381 cagtaataaa cggcgtcaaa gtggttgcag ccggcacaca cgagtcgtgt ttatcaactc
     1441 aaagcacaaa tacttttcct caacctaaaa ataaggcaat tagccaaaaa caactttgcg
     1501 tgtaaacaac gctcaataca cgtgtcattt tattattagc tattgcttca ccgccttagc
     1561 tttctcgtga cctagtcgtc ctcgtctttt cttcttcttc ttctataaaa caatacccaa
     1621 agagctcttc ttcttcacaa ttcagatttc aatttctcaa aatcttaaaa actttctctc
     1681 aattctctct accgtgatca aggtaaattt ctgtgttcct tattctctca aaatcttcga
     1741 ttttgttttc gttcgatccc aatttcgtat atgttctttg gtttagattc tgttaatctt
     1801 agatcgaaga cgattttctg ggtttgatcg ttagatatca tcttaattct cgattagggt
     1861 ttcatagata tcatccgatt tgttcaaata atttgagttt tgtcgaataa ttactcttcg
     1921 atttgtgatt tctatctaga tctggtgtta gtttctagtt tgtgcgatcg aatttgtcga
     1981 ttaatctgag tttttctgat taacagaatg gctactaatc tttctagagt tgctggactt
     2041 gaaaagcaac ttgctattga acttaaggtt aagcaaggtg ctgaaaatat gattcaaact
     2101 tattctaatg gatctactaa ggatagaaag cttcttctta ctgctcaaca aatgcttcaa
     2161 gattctaaga ctaagattga tattattgca atgcaacttg caagagctct tcaagctggt
     2221 caacttgaaa atcaagctgc tcctggttcg atggtgagca agggcgagga gctgttcacc
     2281 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     2341 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     2401 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     2461 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     2521 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     2581 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     2641 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     2701 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     2761 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     2821 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     2881 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     2941 actctcggca tggacgagct gtacaagtga gcttaaatca ccagtctctc tctacaaatc
     3001 tatctctctc tataataatg tgtgagtagt tcccagataa gggaattagg gttcttatag
     3061 ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat
     3121 acttctatca ataaaatttc taattcctaa aaccaaaatc cagcgctgtc atgagacgaa
     3181 ttctgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg
     3241 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
     3301 acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt
     3361 cggcttctga cgttcagtgc agccgtcatc tgaaaacgac atgtcgcaca agtcctaagt
     3421 tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg
     3481 cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc
     3541 cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg
     3601 ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag
     3661 gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac
     3721 agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg
     3781 catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac
     3841 gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct
     3901 aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg
     3961 cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga
     4021 aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg
     4081 cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg
     4141 tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga
     4201 acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc
     4261 gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacct ctcaaccgtg
     4321 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc
     4381 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
     4441 agcttgcgtc atgcggtcgc tgcgtatatg atccgatgag taaataaaca aatacgcaag
     4501 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
     4561 tcggaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
     4621 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
     4681 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
     4741 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag
     4801 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc
     4861 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg
     4921 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg
     4981 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg
     5041 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc
     5101 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat
     5161 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc
     5221 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc
     5281 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct
     5341 gctatctgaa tagatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat
     5401 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg
     5461 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct
     5521 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga cggtcgcaaa
     5581 ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag
     5641 gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg
     5701 caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg
     5761 tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat
     5821 gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag
     5881 cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt
     5941 tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt
     6001 tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc
     6061 gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag
     6121 cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag
     6181 cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt
     6241 agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta
     6301 gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac
     6361 cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc
     6421 gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc
     6481 gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg
     6541 ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac
     6601 cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg
     6661 caaattgccc tagcagggga aaaaggtcga aaaggactct ttcctgtgga tagcacgtac
     6721 attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg
     6781 tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt
     6841 tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa
     6901 ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc
     6961 tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct
     7021 ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc
     7081 cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
     7141 acacatgcag ctcccggtga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     7201 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
     7261 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg
     7321 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     7381 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7441 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7501 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7561 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7621 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7681 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7741 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7801 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7861 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7921 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7981 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     8041 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8101 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8161 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8221 attttggtca tgcattctag gtgattagaa aaactcatcg agcatcaaat gaaactgcaa
     8281 tttattcata tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg
     8341 agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc
     8401 gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag
     8461 tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc
     8521 tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac
     8581 caaaccgtta ttcattcgtg attgcgcctg agcgagtcga aatacgcgat cgctgttaaa
     8641 aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac
     8701 aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt tccctgggat
     8761 cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag
     8821 aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac
     8881 gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcggta
     8941 gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc
     9001 atccatgttg gaatttaatc gcggccttga gcaagacgtt tcccgttgaa tatggctcat
     9061 aacagaactt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta
     9121 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga
     9181 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga
     9241 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta
     9301 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag
     9361 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg
     9421 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg
     9481 ttggctggct ggtggcagga tatattgtgg tgtaaaca
//
