LOCUS       Exported                2826 bp ds-DNA     circular SYN 03-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   L Huang, M Rojas-Pierce, NC State U
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 3, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    21..24
                     /label=AATG
     CDS             22..741
                     /codon_start=1
                     /label=GFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    742..745
                     /label=GCTT
                     /label=GB B5-B6
     rep_origin      complement(1035..1623)
                     /direction=LEFT
                     /label=pMB1
     CDS             complement(1921..2580)
                     /label=CamR
ORIGIN
        1 tagagcgatg agtggtctcg aatggtgagc aagggcgagg agctgttcac cggggtggtg
       61 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      121 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      181 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
      241 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
      301 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
      361 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      421 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
      481 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
      541 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
      601 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
      661 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
      721 atggacgagc tgtacaagta agctttgaga ccactcatcg cgactagtag cggccgctgc
      781 agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa accgaaaaga
      841 ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      901 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      961 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     1021 gcgttgctgg cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc
     1081 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     1141 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     1201 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     1261 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1321 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     1381 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     1441 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     1501 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1561 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     1621 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     1681 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     1741 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagctcgagg
     1801 cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga
     1861 tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa
     1921 ttacgccccg ccctgccact cgtcgcagta ctgttgtaat tcattaagca ttctgccgac
     1981 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
     2041 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
     2101 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgatacga aaaacatatt
     2161 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
     2221 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagtg atgaaaacgt
     2281 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
     2341 accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg
     2401 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
     2461 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
     2521 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
     2581 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
     2641 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga gtgccacctg
     2701 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     2761 agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgcggc
     2821 cgcttc
//
