LOCUS       Exported                2345 bp ds-DNA     circular SYN 03-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   L Huang, M Rojas-Pierce, NC State U
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 3, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    21..24
                     /label=AATG
     CDS             25..258
                     /codon_start=1
                     /label=HR1b
                     /note="HR1b aa122-199 from Kezei et al"
                     /translation="ATNLSRVAGLEKQLAIELKVKQGAENMIQTYSNGSTKDRKLLLTA
                     QQMLQDSKTKIDIIRMQLRRALQAGQLENQAAP"
     misc_feature    261..264
                     /label=TTCG
     rep_origin      complement(554..1142)
                     /direction=LEFT
                     /label=pMB1
     CDS             complement(1440..2099)
                     /label=CamR
ORIGIN
        1 tagagcgatg agtggtctcg aatggctact aatctttcta gagttgctgg acttgaaaag
       61 caacttgcta ttgaacttaa ggttaagcaa ggtgctgaaa atatgattca aacttattct
      121 aatggatcta ctaaggatag aaagcttctt cttactgctc aacaaatgct tcaagattct
      181 aagactaaga ttgatattat tagaatgcaa cttagaagag ctcttcaagc tggtcaactt
      241 gaaaatcaag ctgctcctgg ttcgtgagac cactcatcgc gactagtagc ggccgctgca
      301 gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat
      361 tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      421 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      481 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      541 cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      601 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      661 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      721 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      781 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      841 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      901 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
      961 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     1021 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     1081 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1141 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     1201 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     1261 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agctcgaggc
     1321 ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
     1381 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat
     1441 tacgccccgc cctgccactc gtcgcagtac tgttgtaatt cattaagcat tctgccgaca
     1501 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
     1561 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
     1621 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgatacgaa aaacatattc
     1681 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
     1741 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagtga tgaaaacgtt
     1801 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
     1861 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
     1921 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     1981 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     2041 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     2101 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     2161 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacctga
     2221 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca
     2281 gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcgcggcc
     2341 gcttc
//
