LOCUS       Exported                2370 bp ds-DNA     circular SYN 03-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   L Huang, M Rojas-Pierce, NC State U
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 3, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    21..24
                     /label=AGCC
     CDS             25..48
                     /codon_start=1
                     /label=G/S Linker
                     /translation="GGGSSGGG"
     CDS             49..282
                     /codon_start=1
                     /label=HR1b
                     /note="HR1b aa122-199 from Kezei et al"
                     /translation="ATNLSRVAGLEKQLAIELKVKQGAENMIQTYSNGSTKDRKLLLTA
                     QQMLQDSKTKIDIIRMQLRRALQAGQLENQAAP"
     misc_feature    226..228
                     /label=R181
     misc_feature    238..240
                     /label=R185
     misc_feature    286..289
                     /label=GCTT
     rep_origin      complement(579..1167)
                     /direction=LEFT
                     /label=pMB1
     CDS             complement(1465..2124)
                     /label=CamR
ORIGIN
        1 tagagcgatg agtggtctcg agccggaggt ggctcatctg gcggaggtgc tactaatctt
       61 tctagagttg ctggacttga aaagcaactt gctattgaac ttaaggttaa gcaaggtgct
      121 gaaaatatga ttcaaactta ttctaatgga tctactaagg atagaaagct tcttcttact
      181 gctcaacaaa tgcttcaaga ttctaagact aagattgata ttattagaat gcaacttaga
      241 agagctcttc aagctggtca acttgaaaat caagctgctc cttgagcttt gagaccactc
      301 atcgcgacta gtagcggccg ctgcagtccg gcaaaaaagg gcaaggtgtc accaccctgc
      361 cctttttctt taaaaccgaa aagattactt cgcgttatgc aggcttcctc gctcactgac
      421 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
      481 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
      541 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct ccgcccccct
      601 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
      661 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
      721 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
      781 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
      841 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      901 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
      961 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     1021 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1081 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1141 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1201 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     1261 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     1321 taaacttggt ctgacagctc gaggcttgga ttctcaccaa taaaaaacgc ccggcggcaa
     1381 ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg
     1441 agtccaagcg agctcgatat caaattacgc cccgccctgc cactcgtcgc agtactgttg
     1501 taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat
     1561 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
     1621 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
     1681 attggctgat acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
     1741 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
     1801 ttcactccag agtgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
     1861 aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc
     1921 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
     1981 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
     2041 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
     2101 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
     2161 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
     2221 cccgatcaac tcgagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     2281 ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta
     2341 aggatgattt ctggaattcg cggccgcttc
//
