LOCUS       Exported                3753 bp ds-DNA     circular SYN 07-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   L Huang, M Rojas-Pierce, NC State U
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Nov 8, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    21..24
                     /label=AATG
     CDS             22..51
                     /codon_start=1
                     /label=HA
                     /translation="MYPYDVPDYA"
     CDS             52..1668
                     /codon_start=1
                     /label=LRR-NEL
                     /translation="EYDAVWSKWERDAPAGESPGRAAVVQEMRDCLNNGNPVLNVGASG
                     LTTLPDRLPPHITTLVIPDNNLTSLPELPEGLRELEVSGNLQLTSLPSLPQGLQKLWAY
                     NNWLASLPTLPPGLGDLAVSNNQLTSLPEMPPALRELRVSGNNLTSLPALPSGLQKLWA
                     YNNRLTSLPEMSPGLQELDVSHNQLTRLPQSLTGLSSAARVYLDGNPLSVRTLQALRDI
                     IGHSGIRIHFDMAGPSVPREARALHLAVADWLTSAREGEAAQADRWQAFGLEDNAAAFS
                     LVLDRLRETENFKKDAGFKAQISSWLTQLAEDAALRAKTFAMATEATSTAEDRVTHALH
                     QMNNVQLVHNAEKGEYDNNLQGLVSTGREMFRLATLEQIAREKAGTLALVDDVEVYLAF
                     QNKLKESLELTSVTSEMRFFDVSGVTVSDLQAAELQVKTAENSGFSKWILQWGPLHSVL
                     ERKVPERFNALREKQISDYEDTYRKLYDEVLKSSGLVDDTDAERTIGVSAMDSAKKEFL
                     DGLRALVDEVLGSYLTARWRLN"
     misc_feature    1042..1044
                     /label=C492A
     misc_feature    1669..1672
                     /label=TTCG
     rep_origin      complement(1962..2550)
                     /direction=LEFT
                     /label=pMB1
     CDS             complement(2848..3507)
                     /label=CamR
ORIGIN
        1 tagagcgatg agtggtctcg aatgtaccca tacgatgttc cagattacgc tgagtatgat
       61 gctgtctggt caaaatggga aagggatgca ccagcaggag agtcacctgg aagagcagca
      121 gtggttcagg aaatgcgtga ttgccttaat aacggaaatc cagtgcttaa cgtgggagct
      181 tcaggtctta ccacccttcc agaccgtctt ccaccgcata ttacaacact tgttattcca
      241 gataataatc tcacctcttt gcctgagttg ccagaaggac ttagagagct tgaggtgtct
      301 ggaaaccttc agcttacatc tcttccatct cttccgcagg gtcttcagaa gttgtgggcc
      361 tataacaatt ggcttgcctc tcttccgact ttgccgccag gacttgggga tcttgctgtc
      421 tctaataacc agcttacctc tcttccggag atgccgccag cacttagaga gcttagggtg
      481 tctggtaaca accttacctc tcttccggct cttccgtcag gacttcagaa gctttgggca
      541 tataacaata gattgacctc tttgccagag atgtcaccag gtcttcagga acttgatgtc
      601 tctcataatc agcttaccag attgccgcaa tctctcactg gtctttcttc tgctgcaaga
      661 gtttatcttg acgggaatcc actttctgtt agaactcttc aggctcttag agacatcatt
      721 ggacattcag gaatcaggat acacttcgat atggctgggc cttctgtccc tagagaagcc
      781 agagcacttc accttgctgt cgctgactgg cttacttctg caagagaggg ggaagctgcc
      841 caggcagaca gatggcaggc tttcggactt gaagataacg ccgccgcctt ctctcttgtc
      901 cttgacagac ttcgtgagac tgaaaacttc aaaaaagacg ctggatttaa ggcacagata
      961 tcatcttggc ttacacaact tgctgaagat gctgctctta gagcaaaaac ctttgccatg
     1021 gcaacagagg caacatcaac cgctgaggac agagtcacac atgcccttca ccagatgaat
     1081 aacgttcaac ttgttcataa tgcagaaaaa ggggaatacg acaacaatct ccaggggctt
     1141 gtttctactg ggcgtgagat gttcagactt gcaacacttg aacagattgc cagagaaaaa
     1201 gccggaacac ttgctcttgt cgatgacgtt gaggtctatc ttgctttcca gaataagctt
     1261 aaggaatcac ttgagcttac ctctgtgact tcagaaatgc gtttctttga cgtttctgga
     1321 gtgactgttt cagaccttca ggctgctgag cttcaggtga aaaccgctga aaactctggg
     1381 ttcagtaaat ggatacttca gtgggggccg cttcactctg tgcttgaaag aaaagtgccg
     1441 gaaagattta acgctcttcg tgaaaagcaa atatctgatt atgaagacac ttacagaaag
     1501 ctttatgacg aagtgcttaa atcttctggg cttgtcgacg ataccgatgc agaacgtact
     1561 atcggagtta gtgctatgga tagtgctaaa aaagaatttc ttgatggact tagagctctt
     1621 gtggatgagg tgcttggttc ttatcttaca gccagatggc gtcttaactt cgtgagacca
     1681 ctcatcgcga ctagtagcgg ccgctgcagt ccggcaaaaa agggcaaggt gtcaccaccc
     1741 tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc ctcgctcact
     1801 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     1861 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     1921 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag gctccgcccc
     1981 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     2041 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2101 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     2161 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2221 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     2281 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     2341 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     2401 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     2461 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     2521 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     2581 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     2641 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     2701 gagtaaactt ggtctgacag ctcgaggctt ggattctcac caataaaaaa cgcccggcgg
     2761 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac
     2821 aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcgt cgcagtactg
     2881 ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg
     2941 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac
     3001 gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca
     3061 gggattggct gatacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt
     3121 ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg
     3181 gtattcactc cagagtgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg
     3241 gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg
     3301 agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt
     3361 ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg
     3421 agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt
     3481 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa
     3541 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
     3601 gtgcccgatc aactcgagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     3661 cctataaaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg
     3721 ctaaggatga tttctggaat tcgcggccgc ttc
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