LOCUS       VEI030_pAAV_Syn_        5628 bp DNA     circular SYN 21-JAN-2022
DEFINITION  .
ACCESSION   VB211221-1394kzp
VERSION     .
KEYWORDS    VB211221-1394kzp.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 23, 2023 from SnapGene 6.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5628
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     misc_feature    1..141
                     /label=5' ITR
                     /label=nonstandard type:  ITR
                     /note="/application_notes=Allows rescue of virus from 
                     recombinantplasmid and replication of the viral genome; 
                     this ITR is identical to that of the wild-type AAV2 
                     genome."
                     /note="/description=AAV 5' inverted terminal repeat 
                     (functionalequivalent of wild-type 5' ITR)"
                     /note="/official_designation=5' ITR"
                     /note="/uuid=5f14ec1a645a407dab2725d71bd1c86a"
                     /note="color: #512bbd; direction: RIGHT"
     promoter        169..637
                     /label=SYN1
                     /note="/application_notes=Tissue specificity: Brain. Cell 
                     typespecificity: Mature neurons."
                     /note="/description=Human synapsin I promoter"
                     /note="/full_name=Human synapsin I promoter"
                     /note="/official_designation=SYN1"
                     /note="/uuid=6f036b2e82244653b8e92867f598e473"
                     /note="color: #d84e4e; direction: RIGHT"
     misc_feature    662..667
                     /label=Kozak
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Facilitates translation 
                     initiation ofATG start codon downstream of the Kozak 
                     sequence."
                     /note="/description=Kozak translation initiation sequence"
                     /note="/full_name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
                     /note="color: #1f36a9; direction: RIGHT"
     CDS             680..697
                     /codon_start=1
                     /label=6x His tag
                     /note="color: #c0c0c0"
                     /translation="HHHHHH"
     CDS             701..733
                     /codon_start=1
                     /label=T7 tag
                     /note="color: #c0c0c0"
                     /translation="MASMTGGQQMG"
     CDS             737..760
                     /codon_start=1
                     /label=XPress tag
                     /note="color: #c0c0c0"
                     /translation="DLYDDDDK"
     CDS             776..2017
                     /codon_start=1
                     /label=T-GECO
                     /note="color: #3366ff"
                     /translation="VVSKGDETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     THTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPDYFKQSFPEGYSWERIMTF
                     EDKGIVKVKSDISMEEGSFIYEIYLKGENFPPNGPVMQKKTTGWRNRDQLTEEQIAEFK
                     EAFSLFDKDGDGTITTEELGTVMRSLGQNPTEAELQDMINVVDADGDGAIDFPEFLTMM
                     SRKMNYRDTEEEIREAFGVFDKDGNGYISAAELHRVMTNLGEKLSDEEVDDVIREADID
                     GDGLVNYEEFVQMMTAKGGGGSVDSSRHKWNKTGHAVRAIGRLSSIGGEKMYVRDGVLK
                     GDVEHELLLKGGGHHRVDFKTIYRAKKAVKLPDYHFVDHHIEILNHDKDYNKVTVYESA
                     AARNSRRGMDELYK"
     misc_feature    2048..2645
                     /label=WPRE
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Enhances virus stability in 
                     packagingcells, leading to higher titer of packaged virus; 
                     enhances higher expression of transgenes."
                     /note="/description=Woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
                     /note="/full_name=Woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
                     /note="color: #db6901; direction: RIGHT"
     polyA_signal    2676..2883
                     /label=BGH pA
                     /note="/application_notes=Allows transcription termination 
                     andpolyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=Bovine growth hormone polyadenylation 
                     signal"
                     /note="/full_name=Bovine growth hormone polyadenylation"
                     /note="/official_designation=BGH pA"
                     /note="/uuid=4a09734ca6c745b3aadc757c1638b651"
                     /note="color: #800c0c; direction: RIGHT"
     misc_feature    complement(2891..3031)
                     /label=3' ITR
                     /label=nonstandard type:  ITR
                     /note="/application_notes=Allows rescue of virus from 
                     recombinantplasmid and replication of the viral genome; 
                     this ITR is identical to that of the wild-type AAV2 
                     genome."
                     /note="/description=AAV 3' inverted terminal repeat"
                     /note="/official_designation=3' ITR"
                     /note="/uuid=5b8101593b634e64aa9eef9434216f71"
                     /note="color: #0c5d90; direction: LEFT"
     misc_feature    3948..4808
                     /label=AmpiciIIin
                     /label=nonstandard type:  ORF
                     /note="/application_notes=Allows E. coli to be resistant 
                     toampiciIIin."
                     /note="/description=AmpiciIIin resistance gene"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=5bcc09480c0e4c07b95ddccd8242d769"
                     /note="color: #f58600; direction: RIGHT"
     rep_origin      4979..5567
                     /direction=RIGHT
                     /label=pUC ori
                     /note="/application_notes=Facilitates plasmid replication 
                     in E.coli; regulates high-copy plasmid number (500-700)."
                     /note="/description=pUC origin of replication"
                     /note="/full_name=pUC origin of replication"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=b978ad4ff785460db448caf63b728237"
                     /note="color: #5566f5"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc ttctagacaa ctttgtatag aaaagttgct gcagagggcc
      181 ctgcgtatga gtgcaagtgg gttttaggac caggatgagg cggggtgggg gtgcctacct
      241 gacgaccgac cccgacccac tggacaagca cccaaccccc attccccaaa ttgcgcatcc
      301 cctatcagag agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc
      361 agcaccgcgg acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac
      421 tgaaggcgcg ctgacgtcac tcgccggtcc cccgcaaact ccccttcccg gccaccttgg
      481 tcgcgtccgc gccgccgccg gcccagccgg accgcaccac gcgaggcgcg agataggggg
      541 gcacgggcgc gaccatctgc gctgcggcgc cggcgactca gcgctgcctc agtctgcggt
      601 gggcagcgga ggagtcgtgt cgtgcctgag agcgcagcaa gtttgtacaa aaaagcaggc
      661 tgccaccatg gggggttctc atcatcatca tcatcatggt atggctagca tgactggtgg
      721 acagcaaatg ggtcgggatc tgtacgacga tgacgataag gatccgagct cgagagtggt
      781 gagcaagggc gatgagacca caatgggcgt aatcaagccc gacatgaaga tcaagctgaa
      841 gatggagggc aacgtgaatg gccacgcctt cgtgatcgag ggcgagggcg agggcaagcc
      901 ctacgacggc acccacacca tcaacctgga ggtgaaggag ggagcccccc tgcccttctc
      961 ctacgacatc ctgaccaccg cgttcgccta cggcaacagg gccttcacca agtaccccga
     1021 cgacatcccc gactacttca agcagtcctt ccccgagggc tactcttggg agcgcatcat
     1081 gaccttcgag gacaagggca tcgtgaaggt gaagtccgac atctcgatgg aggagggctc
     1141 cttcatctac gaaatatacc tcaagggcga gaacttcccc cccaacggtc ccgtgatgca
     1201 gaagaagacc accggctggc ggaatcgtga tcaactgact gaagagcaga tcgcagaatt
     1261 taaagaggct ttctccctat ttgataagga cggggatgga acaataacaa ccgaggagct
     1321 ggggacggtg atgcggtctc tggggcagaa ccccacagaa gcagagctgc aggacatgat
     1381 caatgtagta gatgccgacg gtgacggcgc aatcgacttc cctgagttcc tgacaatgat
     1441 gtcaagaaaa atgaactaca gggacacgga agaagaaatt agagaagcgt tcggtgtgtt
     1501 tgataaggat ggcaatggct acatcagtgc agcagagctt caccgcgtga tgacaaacct
     1561 tggagaaaag ttatcagatg aagaggttga tgatgtgatc agggaagcag acatcgatgg
     1621 ggatggtctg gtaaactacg aagagtttgt acaaatgatg acagcgaagg gtggcggagg
     1681 ttctgtcgac tcatcacgtc ataagtggaa taagacaggt cacgcagtca gagctatagg
     1741 tcggctgagc tcaattgggg gtgagaagat gtacgtgcgc gacggcgtgc tgaagggcga
     1801 cgtcgagcac gagctgctgc tgaagggcgg cggccaccac cgcgttgact tcaagaccat
     1861 ctacagggcc aagaaggcgg tgaagctgcc cgactatcac tttgtggacc accacatcga
     1921 gatcctgaac cacgacaagg actacaacaa ggtgaccgtc tacgagagcg ccgcggcccg
     1981 caactcccgt aggggcatgg acgagctgta caagtaaacc cagctttctt gtacaaagtg
     2041 ggaattccga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     2101 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     2161 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     2221 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     2281 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     2341 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     2401 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     2461 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     2521 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     2581 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     2641 atcgggaatt cctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     2701 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     2761 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     2821 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gagaatagca ggcatgctgg
     2881 ggagggccgc aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
     2941 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca
     3001 gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg
     3061 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg
     3121 gcgcattaag cgcggcgggg gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     3181 ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     3241 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     3301 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
     3361 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     3421 ctggaacaac actcaactct atctcgggct attcttttga tttataaggg attttgccga
     3481 tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     3541 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat
     3601 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     3661 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     3721 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     3781 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     3841 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     3901 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     3961 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     4021 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     4081 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     4141 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     4201 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     4261 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     4321 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     4381 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     4441 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     4501 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     4561 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     4621 ctggctggtt tattgctgat aaatctggag ccggtgagcg tggaagccgc ggtatcattg
     4681 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     4741 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     4801 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     4861 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     4921 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     4981 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     5041 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     5101 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     5161 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     5221 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     5281 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     5341 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     5401 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     5461 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     5521 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     5581 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
