LOCUS       Exported                7137 bp ds-DNA     circular SYN 05-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7137)
  AUTHORS   Gaurav Varshney
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, Nov 5, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7137
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    7..12
                     /label=Zebrafish Kozak
     CDS             13..3045
                     /codon_start=1
                     /label=human OGDHL
                     /translation="MSQLRLLPSRLGVQAARLLAAHDVPVFGWRSRSSGPPATFPSSKG
                     GGGSSYMEEMYFAWLENPQSVHKSWDSFFREASEEAFSGSAQPRPPSVVHESRSAVSSR
                     TKTSKLVEDHLAVQSLIRAYQIRGHHVAQLDPLGILDADLDSFVPSDLITTIDKLAFYD
                     LQEADLDKEFQLPTTTFIGGSENTLSLREIIRRLENTYCQHIGLEFMFINDVEQCQWIR
                     QKFETPGVMQFSSEEKRTLLARLVRSMRFEDFLARKWSSEKRFGLEGCEVMIPALKTII
                     DKSSEMGIENVILGMPHRGRLNVLANVIRKDLEQIFCQFDPKLEAADEGSGDVKYHLGM
                     YHERINRVTNRNITLSLVANPSHLEAVDPVVQGKTKAEQFYRGDAQGKKVMSILVHGDA
                     AFAGQGVVYETFHLSDLPSYTTNGTVHVVVNNQIGFTTDPRMARSSPYPTDVARVVNAP
                     IFHVNADDPEAVIYVCSVAAEWRNTFNKDVVVDLVCYRRRGHNEMDEPMFTQPLMYKQI
                     HRQVPVLKKYADKLIAEGTVTLQEFEEEIAKYDRICEEAYGRSKDKKILHIKHWLDSPW
                     PGFFNVDGEPKSMTCPATGIPEDMLTHIGSVASSVPLEDFKIHTGLSRILRGRADMTKN
                     RTVDWALAEYMAFGSLLKEGIHVRLSGQDVERGTFSHRHHVLHDQEVDRRTCVPMNHLW
                     PDQAPYTVCNSSLSEYGVLGFELGYAMASPNALVLWEAQFGDFHNTAQCIIDQFISTGQ
                     AKWVRHNGIVLLLPHGMEGMGPEHSSARPERFLQMSNDDSDAYPAFTKDFEVSQLYDCN
                     WIVVNCSTPANYFHVLRRQILLPFRKPLIIFTPKSLLRHPEAKSSFDQMVSGTSFQRVI
                     PEDGAAARAPEQVQRLIFCTGKVYYDLVKERSSQDLEEKVAITRLEQISPFPFDLIKQE
                     AEKYPGAELAWCQEEHKNMGYYDYISPRFMTILRRARPIWYVGRDPAAAPATGNRNTHL
                     VSLKKFLDTAFNLQAFEGKTF"
     CDS             1094..1095
                     /label=1081C>T; ENSP00000363216.4:p.Pro361Ser
     CDS             1270..1272
                     /label=SNV NM_018245:c.C1259T:p.T420M
     promoter        complement(3069..3087)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3092..3226
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(3339..3357)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3378..3394)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3402..3418
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3426..3456)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3471..3492
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3780..4368)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4539..5399)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5400..5504)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5530..5985)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        6059..7043
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        7078..7096
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaattcgcaa acatgagtca gctgaggctg ctgccgtccc gtcttggggt gcaggctgcg
       61 aggctcctgg ctgcacatga cgtcccggtg tttggctggc gcagcaggtc ctccgggcca
      121 ccggccacct tcccaagcag caaaggtgga ggcggctcca gttacatgga ggagatgtac
      181 ttcgcctggt tggaaaaccc ccagagtgtc cacaagtcct gggacagctt cttcagggaa
      241 gccagcgagg aagccttttc tggctctgct cagccacggc ccccttctgt tgtccatgag
      301 agcaggtctg cagtctcaag tcggaccaag accagcaaat tggtggagga ccacctggct
      361 gtgcagtccc tgatccgggc ctaccagatc cggggtcacc atgtggccca gctggacccc
      421 ctgggcattc tggatgcaga cctggactcc tttgtgccct cagacttgat cacaaccatt
      481 gataaactgg ccttctatga ccttcaggag gctgaccttg ataaggagtt ccagctgccg
      541 acaaccacct tcattggggg ctctgaaaac accctttctc tgcgggagat cattcggcgc
      601 ctggagaaca cctactgcca gcacattggc ctggagttca tgttcatcaa cgatgtggag
      661 cagtgccagt ggatccggca gaagtttgag acccctggtg tgatgcagtt ctccagcgag
      721 gagaagcgga ccctgctggc ccggctagtg cgctccatga ggtttgaaga cttcctggcc
      781 cggaaatggt cctcagagaa gcggtttggc ctggagggct gtgaagtgat gattcctgcc
      841 ctcaagacca tcatcgacaa atccagcgag atggggattg agaatgtcat cttggggatg
      901 ccacacaggg gaaggctgaa cgtgctggcc aacgtgatcc gcaaggacct ggagcagatc
      961 ttctgccagt ttgaccccaa gctggaggcg gcggacgagg gctccgggga tgtcaagtac
     1021 cacctgggca tgtaccatga gaggatcaac cgcgtcacca accggaacat cactctgtcg
     1081 ctggttgcca acccctccca cctggaggca gtggaccctg tggtgcaggg gaagacaaag
     1141 gcagagcagt tctaccgtgg agatgcccag ggcaagaagg tcatgtccat cctggttcat
     1201 ggggacgccg cctttgctgg ccagggcgtg gtatatgaga ccttccacct gagcgacctg
     1261 ccctcctaca cgaccaatgg taccgtgcac gtcgtcgtca acaaccagat tggattcacc
     1321 acagaccccc gaatggcccg ctcctcacca tacccgaccg acgtggcccg ggtggtcaat
     1381 gcgcctatct tccatgtgaa tgcagatgac ccagaggctg tgatatatgt gtgcagtgtg
     1441 gcagccgaat ggagaaacac tttcaacaaa gatgttgtcg tggacctggt ctgttaccgc
     1501 cggcgtggcc acaatgagat ggacgagccc atgttcaccc agccgctcat gtacaagcag
     1561 atccacagac aggtgcctgt gctgaagaag tacgcagaca agctgattgc cgagggcaca
     1621 gtcaccctgc aggagtttga ggaagaaatt gccaaatacg accggatctg tgaggaggct
     1681 tatggcaggt ccaaggataa aaagattctg catataaagc actggttgga ctccccctgg
     1741 cctggcttct tcaacgtaga tggggagccc aagagcatga catgcccagc cacggggatc
     1801 cctgaggaca tgctcaccca catcggcagt gtggccagct ctgtgcccct ggaggacttt
     1861 aagatccaca ctggcctctc tcgcattctg cggggccgtg cggacatgac caagaaccgg
     1921 acggtggact gggcgttggc agagtacatg gcctttggct ccctgctgaa ggaaggcatc
     1981 cacgtgcggc tcagcgggca ggatgtggag aggggcacat tcagtcaccg gcaccatgtt
     2041 ctccatgacc aggaggttga ccgcaggacg tgtgtgccta tgaatcatct ctggcctgac
     2101 caggccccgt acaccgtgtg caacagctcc ctctcggagt acggagtcct gggctttgag
     2161 ctgggctatg ccatggccag ccccaatgcc ctggtcctct gggaggccca gtttggggac
     2221 ttccacaaca cggcccagtg catcatcgac cagttcatca gcaccggcca ggccaagtgg
     2281 gtgcggcata atggcattgt gctgctgctg ccccatggca tggaaggcat gggcccagag
     2341 cactcgtcag cgaggcccga aaggttcctg cagatgagca atgatgactc ggatgcctac
     2401 cctgcattca ccaaggactt cgaggtgagc cagctctatg actgcaactg gatcgtggtc
     2461 aactgctcca caccggccaa ctacttccac gtgctgcgcc ggcagatcct gctgcccttc
     2521 cgcaagccgc tgattatctt cacacctaaa tctctgctga ggcacccaga ggccaagtcc
     2581 agctttgacc aaatggtatc cgggaccagc ttccagcggg tgattcctga agatggggcc
     2641 gcagcacggg cccctgagca ggtgcagcgg ctcatcttct gcacgggaaa ggtgtactat
     2701 gacctggtga aggagcggag cagccaggac ctggaggaga aagtggccat cacgcgcctg
     2761 gagcagatct ctccattccc cttcgacctg atcaagcagg aggcagagaa gtacccaggt
     2821 gcggagctgg cctggtgtca ggaggagcac aagaacatgg gctactatga ctacatcagc
     2881 ccacgcttca tgaccatcct gaggcgcgca cggcccatat ggtatgttgg ccgggaccca
     2941 gcggctgcac cagccacagg aaacaggaac actcacctgg tgtcactgaa gaagtttctg
     3001 gatactgcct tcaatctcca ggcctttgag ggcaagacat tttagctcga gcctctagat
     3061 tctgcagccc tatagtgagt cgtattacgt agatccagac atgataagat acattgatga
     3121 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     3181 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     3241 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaat tcgcggccgc
     3301 ggcgccaatg cattgggccc ggtacccagc ttttgttccc tttagtgagg gttaattgcg
     3361 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     3421 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     3481 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     3541 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     3601 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     3661 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     3721 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     3781 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     3841 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     3901 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     3961 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     4021 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     4081 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     4141 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     4201 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     4261 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     4321 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     4381 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     4441 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     4501 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     4561 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     4621 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     4681 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     4741 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     4801 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     4861 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     4921 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     4981 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     5041 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     5101 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     5161 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     5221 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     5281 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     5341 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     5401 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     5461 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     5521 gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     5581 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     5641 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     5701 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     5761 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     5821 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     5881 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     5941 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca
     6001 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagtcga
     6061 ccatagccaa ttcaatatgg cgtatatgga ctcatgccaa ttcaatatgg tggatctgga
     6121 cctgtgccaa ttcaatatgg cgtatatgga ctcgtgccaa ttcaatatgg tggatctgga
     6181 ccccagccaa ttcaatatgg cggacttggc accatgccaa ttcaatatgg cggacttggc
     6241 actgtgccaa ctggggaggg gtctacttgg cacggtgcca agtttgagga ggggtcttgg
     6301 ccctgtgcca agtccgccat attgaattgg catggtgcca ataatggcgg ccatattggc
     6361 tatatgccag gatcaatata taggcaatat ccaatatggc cctatgccaa tatggctatt
     6421 ggccaggttc aatactatgt attggcccta tgccatatag tattccatat atgggttttc
     6481 ctattgacgt agatagcccc tcccaatggg cggtcccata taccatatat ggggcttcct
     6541 aataccgccc atagccactc ccccattgac gtcaatggtc tctatatatg gtctttccta
     6601 ttgacgtcat atgggcggtc ctattgacgt atatggcgcc tcccccattg acgtcaatta
     6661 cggtaaatgg cccgcctggc tcaatgccca ttgacgtcaa taggaccacc caccattgac
     6721 gtcaatggga tggctcattg cccattcata tccgttctca cgccccctat tgacgtcaat
     6781 gacggtaaat ggcccacttg gcagtacatc aatatctatt aatagtaact tggcaagtac
     6841 attactattg gaaggacgcc agggtacatt ggcagtactc ccattgacgt caatggcggt
     6901 aaatggcccg cgatggctgc caagtacatc cccattgacg tcaatgggga ggggcaatga
     6961 cgcaaatggg cgttccattg acgtaaatgg gcggtaggcg tgcctaatgg gaggtctata
     7021 taagcaatgc tcgtttaggg aaccgccatt ctgcctgggg acgtcggagc aagcttgatt
     7081 taggtgacac tatagaatac aagctacttg ttctttttgc aggatcccat cgattcg
//
