LOCUS       Plasmid_2B:_pSL1119_[Ca 4790 bp ds-DNA     circular     08-NOV-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     complement(204..223)
                     /label="pRS-marker"
     primer_bind     323..345
                     /label="pGEX 3'"
     primer_bind     complement(383..401)
                     /label="pBRforEco"
     promoter        469..573
                     /label="AmpR promoter"
     CDS             574..1434
                     /label="AmpR"
     primer_bind     complement(792..811)
                     /label="Amp-R"
     misc_feature    1555..1555
                     /label="Conserved T"
     misc_feature    1555..1576
                     /label="N1"
     misc_feature    1556..1575
                     /label="N1 design"
     misc_feature    1576..1576
                     /label="Conserved G"
     CDS             complement(1577..2527)
                     /label="repA101"
     CDS             complement(1577..2527)
                     /label="Translation 2527-1577"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKERTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFSSEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANIEISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQVELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKFLSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI*"
     misc_feature    2531..3271
                     /label="ori101"
     misc_feature    3272..3272
                     /label="Conserved T"
     misc_feature    3272..3294
                     /label="N2"
     misc_feature    3273..3293
                     /label="N2 design"
     misc_feature    3294..3294
                     /label="Conserved G"
     primer_bind     3360..3377
                     /label="L4440"
     misc_feature    3452..3459
                     /label="END"
     misc_feature    3452..3479
                     /label="Right flank"
     misc_feature    3452..3576
                     /label="VchINT R-end"
     misc_feature    complement(3460..3479)
                     /label="TnsB_1?"
     misc_feature    complement(3480..3499)
                     /label="TnsB_2?"
     misc_feature    complement(3503..3522)
                     /label="TnsB_3?"
     misc_feature    complement(3516..3535)
                     /label="TnsB_4?"
     misc_feature    3577..3577
                     /label="Conserved T"
     misc_feature    3577..3601
                     /label="T7p Barcode"
     misc_feature    3578..3600
                     /label="T7p design"
     misc_feature    3601..3601
                     /label="Conserved G"
     promoter        3603..3627
                     /label="T7p"
     misc_feature    3625..3647
                     /label="LacO"
     misc_feature    3654..3696
                     /label="RBS1"
     CDS             3697..4407
                     /label="pure\eGFP"
     CDS             3697..4407
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     misc_feature    4408..4427
                     /label="N31"
     misc_feature    4428..4584
                     /label="pheA terminator fragment"
     misc_feature    4585..3601
                     /label="Fragment 1"
     misc_feature    4585..4605
                     /label="N23"
     misc_feature    complement(4606..4750)
                     /label="VchINT L-end"
     misc_feature    4643..4662
                     /label="TnsB_3?"
     misc_feature    4669..4688
                     /label="TnsB_2?"
     misc_feature    4723..4742
                     /label="TnsB_1?"
     misc_feature    4723..4750
                     /label="Left flank"
     misc_feature    complement(4743..4750)
                     /label="END"
     misc_feature    4752..4768
                     /label="MCS"
     primer_bind     complement(4769..4785)
                     /label="M13 fwd"
     primer_bind     complement(4769..4786)
                     /label="M13 Forward"
     primer_bind     complement(4778..10)
                     /label="M13/pUC Forward"
ORIGIN
        1 CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC
       61 GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC
      121 CTGAATGGCG AATGGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA
      181 CACCGCATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGCCC
      241 CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT
      301 TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA
      361 CCGAAACGCG CGAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG
      421 ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT
      481 ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA
      541 TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC
      601 CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG
      661 AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC
      721 AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT
      781 TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC
      841 GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG
      901 CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT
      961 AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT
     1021 TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA
     1081 GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC
     1141 AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG
     1201 GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT
     1261 GCTGATAAAT CTGGAGCCGG TGAGCGTGGc TCTCGCGGTA TCATTGCAGC ACTGGGGCCA
     1321 GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT
     1381 GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA
     1441 GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG
     1501 ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG
     1561 TTCCACTGAG CGTCAGtcag atccttccgt atttagccag tatgttctct agtgtggttc
     1621 gttgtttttg cgtgagccat gagaacgaac cattgagatc atacttactt tgcatgtcac
     1681 tcaaaaattt tgcctcaaaa ctggtgagct gaatttttgc agttaaagca tcgtgtagtg
     1741 tttttcttag tccgttacgt aggtaggaat ctgatgtaat ggttgttggt attttgtcac
     1801 cattcatttt tatctggttg ttctcaagtt cggttacgag atccatttgt ctatctagtt
     1861 caacttggaa aatcaacgta tcagtcgggc ggcctcgctt atcaaccacc aatttcatat
     1921 tgctgtaagt gtttaaatct ttacttattg gtttcaaaac ccattggtta agccttttaa
     1981 actcatggta gttattttca agcattaaca tgaacttaaa ttcatcaagg ctaatctcta
     2041 tatttgcctt gtgagttttc ttttgtgtta gttcttttaa taaccactca taaatcctca
     2101 tagagtattt gttttcaaaa gacttaacat gttccagatt atattttatg aattttttta
     2161 actggaaaag ataaggcaat atctcttcac taaaaactaa ttctaatttt tcgcttgaga
     2221 acttggcata gtttgtccac tggaaaatct caaagccttt aaccaaagga ttcctgattt
     2281 ccacagttct cgtcatcagc tctctggttg ctttagctaa tacaccataa gcattttccc
     2341 tactgatgtt catcatctga gcgtattggt tataagtgaa cgataccgtc cgttctttcc
     2401 ttgtagggtt ttcaatcgtg gggttgagta gtgccacaca gcataaaatt agcttggttt
     2461 catgctccgt taagtcatag cgactaatcg ctagttcatt tgctttgaaa acaactaatt
     2521 cagacataca tctcaattgg tctaggtgat tttaatcact ataccaattg agatgggcta
     2581 gtcaatgata attactagtc cttttccttt gagttgtggg tatctgtaaa ttctgctaga
     2641 cctttgctgg aaaacttgta aattctgcta gaccctctgt aaattccgct agacctttgt
     2701 gtgttttttt tgtttatatt caagtggtta taatttatag aataaagaaa gaataaaaaa
     2761 agataaaaag aatagatccc agccctgtgt ataactcact actttagtca gttccgcagt
     2821 attacaaaag gatgtcgcaa acgctgtttg ctcctctaca aaacagacct taaaacccta
     2881 aaggcttaag tagcaccctc gcaagctcgg ttgcggccgc aatcgggcaa atcgctgaat
     2941 attccttttg tctccgacca tcaggcacct gagtcgctgt ctttttcgtg acattcagtt
     3001 cgctgcgctc acggctctgg cagtgaatgg gggtaaatgg cactacaggc gccttttatg
     3061 gattcatgca aggaaactac ccataataca agaaaagccc gtcacgggct tctcagggcg
     3121 ttttatggcg ggtctgctat gtggtgctat ctgacttttt gctgttcagc agttcctgcc
     3181 ctctgatttt ccagtctgac cacttcggat tatcccgtga caggtcattc agactggcta
     3241 atgcacccag taaggcagcg gtatcatcaa cTCCTGCGTT ATCCCCTGAT TCTGTGGATA
     3301 ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACT ACCGAGCGCA
     3361 GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC
     3421 GTTGGCCGAT TCATTAATGC AGCCCAAGCT TTGTTGATAC AACCATAAAA TGATAATTAC
     3481 ACCCATAAAT TGATAATTAT CACACCCATA AATTGATATT GCCTCTTCAT GGTCTAAACT
     3541 TCAGTAAGTT TACGACATTT TCCTCGAGGT CATTTCtccc ttatgcgact cctgcattag
     3601 gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc ctcTagaaat
     3661 aattttgttt aactttaaga aggagatata catatGagca agggcgagga gctgttcacc
     3721 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     3781 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     3841 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     3901 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     3961 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     4021 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     4081 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     4141 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     4201 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     4261 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     4321 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     4381 actctcggca tggacgagct gtacaagTga TGGGCTGAAG GGTTTaaGcg aagacgaaca
     4441 ataaggcctc ccaaatcggg gggccttttt tattgataac aaaaaggcaa cactatgaca
     4501 tcggaaaacc cgttactggc gctgcgagag aaaatcagcg cgctggatga aaaattatta
     4561 gcattactgg cagagcggcg cgaaTtCAAA CAGAAAGGCC ATggGCTTAC TGCAGTAGTT
     4621 TTGCTGAAAT ACTCGATTCA CAAAAATATC AACTTATGGT TGTTTTGTGA GATATCAATA
     4681 TATGGTTGTT TTGTGGTTAA GTTGCTGATT ATAAATAATT ATTAAATATC ACTTTATGGT
     4741 TGCATCAACA GGTACCGAGC TCGAATTCAC TGGCCGTCGT TTTACAACGT
//