LOCUS       Plasmid_2C:_pSL1119_[Ca 4935 bp ds-DNA     circular     02-NOV-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    18..18
                     /label="Conserved T"
     misc_feature    18..40
                     /label="N2"
     misc_feature    19..39
                     /label="N2 design"
     misc_feature    40..40
                     /label="Conserved G"
     primer_bind     106..123
                     /label="L4440"
     misc_feature    198..205
                     /label="END"
     misc_feature    198..225
                     /label="Right flank"
     misc_feature    198..322
                     /label="VchINT R-end"
     misc_feature    complement(206..225)
                     /label="TnsB_1?"
     misc_feature    complement(226..245)
                     /label="TnsB_2?"
     misc_feature    complement(249..268)
                     /label="TnsB_3?"
     misc_feature    complement(262..281)
                     /label="TnsB_4?"
     misc_feature    323..343
                     /label="N21"
     misc_feature    323..1318
                     /label="Fragment 2"
     misc_feature    344..543
                     /label="PgyrA"
     misc_feature    416..425
                     /label="cspa trans start"
     misc_feature    527..533
                     /label="Ppnp trans start"
     misc_feature    544..586
                     /label="RBS1"
     CDS             584..586
                     /label="Translation 584-586"
                     /translation="M"
     misc_feature    587..1297
                     /label="gfp"
     CDS             587..1297
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     misc_feature    1298..343
                     /label="Fragment 1"
     misc_feature    1298..1318
                     /label="N31"
     misc_feature    1319..1475
                     /label="pheA terminator fragment"
     misc_feature    1476..1496
                     /label="N23"
     misc_feature    complement(1497..1641)
                     /label="VchINT L-end"
     misc_feature    1534..1553
                     /label="TnsB_3?"
     misc_feature    1560..1579
                     /label="TnsB_2?"
     misc_feature    1614..1633
                     /label="TnsB_1?"
     misc_feature    1614..1641
                     /label="Left flank"
     misc_feature    complement(1634..1641)
                     /label="END"
     misc_feature    1643..1659
                     /label="MCS"
     primer_bind     complement(1660..1676)
                     /label="M13 fwd"
     primer_bind     complement(1660..1677)
                     /label="M13 Forward"
     primer_bind     complement(1669..1691)
                     /label="M13/pUC Forward"
     primer_bind     complement(1885..1904)
                     /label="pRS-marker"
     primer_bind     2004..2026
                     /label="pGEX 3'"
     primer_bind     complement(2064..2082)
                     /label="pBRforEco"
     promoter        2150..2254
                     /label="AmpR promoter"
     CDS             2255..3115
                     /label="AmpR"
     primer_bind     complement(2473..2492)
                     /label="Amp-R"
     misc_feature    3236..3236
                     /label="Conserved T"
     misc_feature    3236..3257
                     /label="N1"
     misc_feature    3237..3256
                     /label="N1 design"
     misc_feature    3257..3257
                     /label="Conserved G"
     CDS             complement(3258..4208)
                     /label="repA101"
     CDS             complement(3258..4208)
                     /label="Translation 4208-3258"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKERTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFSSEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANIEISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQVELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKFLSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI*"
     misc_feature    4212..17
                     /label="ori101"
ORIGIN
        1 gcagcggtat catcaacTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC
       61 TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACTACCG AGCGCAGCGA GTCAGTGAGC
      121 GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT
      181 TAATGCAGCC CAAGCTTTGT TGATACAACC ATAAAATGAT AATTACACCC ATAAATTGAT
      241 AATTATCACA CCCATAAATT GATATTGCCT CTTCATGGTC TAAACTTCAG TAAGTTTACG
      301 ACATTTTCCT CGAGGTCATT TCTTccctcg actcacactt ggGgaggcga cggcttcaaa
      361 tttagcgatc tcttcgtggt ctacgttatg gtttTccggc gatttttcgg cattcattgg
      421 cacttctact ccgtaattgg caagacaaac gagtatatca ggcattggat gtgaataaag
      481 cgtataggtt tacctcaaac tgcgcggctg tgttataatt tgcgaccttt gaatccggga
      541 tactagaaat aattttgttt aactttaaga aggagatata catatgagca agggcgagga
      601 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
      661 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
      721 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
      781 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
      841 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
      901 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
      961 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     1021 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     1081 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     1141 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     1201 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     1261 cgccgggatc actctcggca tggacgagct gtacaagTga tgggctgaag ggtttaaGGc
     1321 gaagacgaac aataaggcct cccaaatcgg ggggcctttt ttattgataa caaaaaggca
     1381 acactatgac atcggaaaac ccgttactgg cgctgcgaga gaaaatcagc gcgctggatg
     1441 aaaaattatt agcattactg gcagagcggc gcgaaTtCAA ACAGAAAGGC CATggGCTTA
     1501 CTGCAGTAGT TTTGCTGAAA TACTCGATTC ACAAAAATAT CAACTTATGG TTGTTTTGTG
     1561 AGATATCAAT ATATGGTTGT TTTGTGGTTA AGTTGCTGAT TATAAATAAT TATTAAATAT
     1621 CACTTTATGG TTGCATCAAC AGGTACCGAG CTCGAATTCA CTGGCCGTCG TTTTACAACG
     1681 TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT
     1741 CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG
     1801 CCTGAATGGC GAATGGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC
     1861 ACACCGCATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGCC
     1921 CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC
     1981 TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC
     2041 ACCGAAACGC GCGAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT
     2101 GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC
     2161 TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG
     2221 ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC
     2281 CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT
     2341 GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT
     2401 CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC
     2461 TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT
     2521 CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA
     2581 GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA
     2641 TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT
     2701 TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA
     2761 AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG
     2821 CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT
     2881 GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT
     2941 TGCTGATAAA TCTGGAGCCG GTGAGCGTGG cTCTCGCGGT ATCATTGCAG CACTGGGGCC
     3001 AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA
     3061 TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC
     3121 AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG
     3181 GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC
     3241 GTTCCACTGA GCGTCAGtca gatccttccg tatttagcca gtatgttctc tagtgtggtt
     3301 cgttgttttt gcgtgagcca tgagaacgaa ccattgagat catacttact ttgcatgtca
     3361 ctcaaaaatt ttgcctcaaa actggtgagc tgaatttttg cagttaaagc atcgtgtagt
     3421 gtttttctta gtccgttacg taggtaggaa tctgatgtaa tggttgttgg tattttgtca
     3481 ccattcattt ttatctggtt gttctcaagt tcggttacga gatccatttg tctatctagt
     3541 tcaacttgga aaatcaacgt atcagtcggg cggcctcgct tatcaaccac caatttcata
     3601 ttgctgtaag tgtttaaatc tttacttatt ggtttcaaaa cccattggtt aagcctttta
     3661 aactcatggt agttattttc aagcattaac atgaacttaa attcatcaag gctaatctct
     3721 atatttgcct tgtgagtttt cttttgtgtt agttctttta ataaccactc ataaatcctc
     3781 atagagtatt tgttttcaaa agacttaaca tgttccagat tatattttat gaattttttt
     3841 aactggaaaa gataaggcaa tatctcttca ctaaaaacta attctaattt ttcgcttgag
     3901 aacttggcat agtttgtcca ctggaaaatc tcaaagcctt taaccaaagg attcctgatt
     3961 tccacagttc tcgtcatcag ctctctggtt gctttagcta atacaccata agcattttcc
     4021 ctactgatgt tcatcatctg agcgtattgg ttataagtga acgataccgt ccgttctttc
     4081 cttgtagggt tttcaatcgt ggggttgagt agtgccacac agcataaaat tagcttggtt
     4141 tcatgctccg ttaagtcata gcgactaatc gctagttcat ttgctttgaa aacaactaat
     4201 tcagacatac atctcaattg gtctaggtga ttttaatcac tataccaatt gagatgggct
     4261 agtcaatgat aattactagt ccttttcctt tgagttgtgg gtatctgtaa attctgctag
     4321 acctttgctg gaaaacttgt aaattctgct agaccctctg taaattccgc tagacctttg
     4381 tgtgtttttt ttgtttatat tcaagtggtt ataatttata gaataaagaa agaataaaaa
     4441 aagataaaaa gaatagatcc cagccctgtg tataactcac tactttagtc agttccgcag
     4501 tattacaaaa ggatgtcgca aacgctgttt gctcctctac aaaacagacc ttaaaaccct
     4561 aaaggcttaa gtagcaccct cgcaagctcg gttgcggccg caatcgggca aatcgctgaa
     4621 tattcctttt gtctccgacc atcaggcacc tgagtcgctg tctttttcgt gacattcagt
     4681 tcgctgcgct cacggctctg gcagtgaatg ggggtaaatg gcactacagg cgccttttat
     4741 ggattcatgc aaggaaacta cccataatac aagaaaagcc cgtcacgggc ttctcagggc
     4801 gttttatggc gggtctgcta tgtggtgcta tctgactttt tgctgttcag cagttcctgc
     4861 cctctgattt tccagtctga ccacttcgga ttatcccgtg acaggtcatt cagactggct
     4921 aatgcaccca gtaag
//