LOCUS       PgyrA_-_RBS1_-_GFP_-_N3 3159 bp ds-DNA     circular     16-NOV-2022
DEFINITION  .
COMMENT     G dadA6 F : G*aaatcaatcgctgtcatcggc dadA6 T R : A*agccttggccaatgcca
FEATURES             Location/Qualifiers
     misc_feature    246..266
                     /label="N21"
     misc_feature    249..263
                     /label="SE"
     misc_feature    267..466
                     /label="PgyrA"
     misc_feature    339..348
                     /label="cspa trans start"
     misc_feature    450..456
                     /label="Ppnp trans start"
     misc_feature    467..509
                     /label="RBS1"
     misc_feature    510..1220
                     /label="GFP"
     CDS             510..1220
                     /label="GFP"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     misc_feature    1221..1241
                     /label="N31"
     misc_feature    1242..1397
                     /label="pheA terminator"
     misc_feature    1398..1418
                     /label="N23"
     misc_feature    1400..1415
                     /label="SE"
     misc_feature    1560..1586
                     /label="Inner RBS1 (N21A)"
     CDS             1584..1586
                     /translation="M"
     CDS             1587..2372
                     /label="aadA"
     CDS             1587..2375
                     /label="aadA"
                     /translation="REAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK*"
     misc_feature    2373..2397
                     /label="Inner N31 (N22A)"
     misc_feature    2496..2516
                     /label="N22"
     misc_feature    2499..2513
                     /label="SE"
     CDS             2569..3157
                     /label="pMB1"
ORIGIN
        1 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
       61 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
      121 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      181 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatgctg
      241 gatccTtccc tcgactcaca cttggGgagg cgacggcttc aaatttagcg atctcttcgt
      301 ggtctacgtt atggtttTcc ggcgattttt cggcattcat tggcacttct actccgtaat
      361 tggcaagaca aacgagtata tcaggcattg gatgtgaata aagcgtatag gtttacctca
      421 aactgcgcgg ctgtgttata atttgcgacc tttgaatccg ggatactaga aataattttg
      481 tttaacttta agaaggagat atacatatga gcaagggcga ggagctgttc accggggtgg
      541 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
      601 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
      661 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
      721 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
      781 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
      841 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
      901 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
      961 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     1021 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     1081 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     1141 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     1201 gcatggacga gctgtacaag Tgatgggctg aagggtttaa GCGAAGACGA ACAATAAGGC
     1261 CTCCCAAATC GGGGGGCCTT TTTTATTGAT AACAAAAAGG CAACACTATG ACATCGGAAA
     1321 ACCCGTTACT GGCGCTGCGA GAGAAAATCA GCGCGCTGGA TGAAAAATTA TTAGCATTAC
     1381 TGGCAGAGCG GCGCGAATtc aaacagaaag gccatggGtc gacctgcaga agcttagatc
     1441 tattaccctg ttatccctac tcgagttcat gtgcagctcc atcagcaaaa ggggatgata
     1501 agtttatcac caccgactat ttgcaacagt gccgttgatc gtgctatgat cgactgatgt
     1561 catcagcggt ggagtgcaat gtcatgaggg aagcggtgat cgccgaagta tcgactcaac
     1621 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     1681 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     1741 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     1801 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     1861 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     1921 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     1981 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     2041 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     2101 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     2161 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     2221 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     2281 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
     2341 tgaaaggcga gatcaccaag gtagtcggca aataagatgc cgctcgccag tcgattggct
     2401 gagctcatga agttcctatt ccgaagttcc gcgaacgcgt aaaggatcta ggtgaagatc
     2461 ctttttgata atctcatgac caaaatccct taacgTtcac acaacatagc cacggGagtt
     2521 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     2581 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     2641 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     2701 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     2761 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     2821 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     2881 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     2941 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     3001 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     3061 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     3121 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaa
//