LOCUS       Gly08_(PgyrA_-_RBS1_-_k 5920 bp ds-DNA     circular     04-NOV-2022
DEFINITION  .
COMMENT     G dadA6 F : G*aaatcaatcgctgtcatcggc dadA6 T R : A*agccttggccaatgcca
FEATURES             Location/Qualifiers
     misc_feature    242..261
                     /label="N21"
     misc_feature    262..461
                     /label="PgyrA"
     misc_feature    334..343
                     /label="cspa trans start"
     misc_feature    445..451
                     /label="Ppnp trans start"
     misc_feature    462..504
                     /label="RBS1"
     misc_feature    505..2679
                     /label="katG (E. coli)"
     CDS             505..2679
                     /label="Translation 505-2679"
                     /translation="STSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVDLLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIRMAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWADLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLGATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAGPTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLFKYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRRFLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDIIDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVRALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDIEMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFDGSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKFTASRADLVFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     misc_feature    1378..1396
                     /label="katG inner F"
     misc_feature    complement(1378..1396)
                     /label="katG inner R"
     CDS             2680..2682
                     /label="Translation 2680-2682"
                     /translation="*"
     misc_feature    2680..2714
                     /label="RBS2stop"
     CDS             2712..2714
                     /label="Translation 2712-2714"
                     /translation="M"
     misc_feature    2715..3977
                     /label="dadA6 (Ralstonia eutropha)"
     CDS             2715..3977
                     /label="Translation 2715-3977"
                     /translation="KSIAVIGGGITGVTTAYALARRGFSVTLFERHRYAAMETSFANGGQLSASNAEVWTHWSTLVKGMKWMLRNDAPLLVNPRPSWHKLSWFAEFIAAIPHYRRNTIETARLAIAAREHLFAWAAQEGIDFDLKKEGILHIYRDKTGFEHAGRVSALLAQGGLERRAVTPEEMRAIEPTLAGSYYGGYFTESDSTGDIHKFTSGLAAAIDRLGVRCLYGQDVRAVKTDGRRATVTVQDGADTATSTFDGAVICAGTASRALAAGLGDRVNIYPVKGYSITVNLTDEASRAAAPVVSLLDDETKLVTSRLGDDRFRVAGTAEFNGYNRDIRADRIRPLVEWVQQCFPGVSTRSVVPWAGLRPMMPDMLPRVGRGRAACIFYNTGHGHLGWTLSAVTADMVAGVVDQHVGAKVAVAAGAPMALAKA"
     misc_feature    complement(3119..3137)
                     /label="dadA6 inner R"
     misc_feature    3978..3998
                     /label="N31"
     misc_feature    3999..4154
                     /label="pheA terminator"
     misc_feature    4155..4175
                     /label="N23"
     CDS             4341..5133
                     /label="aadA"
     misc_feature    5253..5273
                     /label="N22"
     CDS             5326..5914
                     /label="pMB1"
ORIGIN
        1 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
       61 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      121 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      181 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atgctggatc
      241 cTccctcgac tcacacttgg Ggaggcgacg gcttcaaatt tagcgatctc ttcgtggtct
      301 acgttatggt ttTccggcga tttttcggca ttcattggca cttctactcc gtaattggca
      361 agacaaacga gtatatcagg cattggatgt gaataaagcg tataggttta cctcaaactg
      421 cgcggctgtg ttataatttg cgacctttga atccgggata ctagaaataa ttttgtttaa
      481 ctttaagaag gagatataca tatgAGCACG TCAGACGATA TCCATAACAC CACAGCCACT
      541 GGCAAATGCC CGTTCCATCA GGGCGGTCAC GACCAGAGTG CGGGGGCGGG CACAACCACT
      601 CGCGACTGGT GGCCAAATCA ACTTCGTGTT GACCTGTTAA ACCAACATTC TAATCGTTCT
      661 AACCCACTGG GTGAGGACTT TGACTACCGC AAAGAATTCA GCAAATTAGA TTACTACGGC
      721 CTGAAAAAAG ATCTGAAAGC CCTGTTGACA GAATCTCAAC CGTGGTGGCC AGCCGACTGG
      781 GGCAGTTACG CCGGTCTGTT TATTCGTATG GCCTGGCACG GCGCGGGGAC TTACCGTTCA
      841 ATCGATGGAC GCGGTGGCGC GGGTCGTGGT CAGCAACGTT TTGCACCGCT GAACTCCTGG
      901 CCGGATAACG TAAGCCTCGA TAAAGCGCGT CGCCTGTTGT GGCCAATCAA ACAGAAATAT
      961 GGTCAGAAAA TCTCCTGGGC CGACCTGTTT ATCCTCGCGG GTAACGTGGC GCTAGAAAAC
     1021 TCCGGCTTCC GTACCTTCGG TTTTGGTGCC GGTCGTGAAG ACGTCTGGGA ACCGGATCTG
     1081 GATGTTAACT GGGGTGATGA AAAAGCCTGG CTGACTCACC GTCATCCGGA AGCGCTGGCG
     1141 AAAGCACCGC TGGGTGCAAC CGAGATGGGT CTGATTTACG TTAACCCGGA AGGCCCGGAT
     1201 CACAGCGGCG AACCGCTTTC TGCGGCAGCA GCTATCCGCG CGACCTTCGG CAACATGGGC
     1261 ATGAACGACG AAGAAACCGT GGCGCTGATT GCGGGTGGTC ATACGCTGGG TAAAACCCAC
     1321 GGTGCCGGTC CGACATCAAA TGTAGGTCCT GATCCAGAAG CTGCACCGAT TGAAGAACAA
     1381 GGTTTAGGTT GGGCGAGCAC TTACGGCAGC GGCGTTGGCG CAGATGCCAT TACCTCTGGT
     1441 CTGGAAGTAG TCTGGACCCA GACGCCGACC CAGTGGAGCA ACTATTTCTT CGAGAACCTG
     1501 TTCAAGTATG AGTGGGTACA GACCCGCAGC CCGGCTGGCG CAATCCAGTT CGAAGCGGTA
     1561 GACGCACCGG AAATTATCCC GGATCCGTTT GATCCGTCGA AGAAACGTAA ACCGACAATG
     1621 CTGGTGACCG ACCTGACGCT GCGTTTTGAT CCTGAGTTCG AGAAGATCTC TCGTCGTTTC
     1681 CTCAACGATC CGCAGGCGTT CAACGAAGCC TTTGCCCGTG CCTGGTTCAA ACTGACGCAC
     1741 AGGGATATGG GGCCGAAATC TCGCTACATC GGGCCGGAAG TGCCGAAAGA AGATCTGATC
     1801 TGGCAAGATC CGCTGCCGCA GCCGATCTAC AACCCGACCG AGCAGGACAT TATCGATCTG
     1861 AAATTCGCGA TTGCGGATTC TGGTCTGTCT GTTAGTGAGC TGGTATCGGT GGCCTGGGCA
     1921 TCTGCTTCTA CCTTCCGTGG TGGCGACAAA CGCGGTGGTG CCAACGGTGC GCGTCTGGCA
     1981 TTAATGCCGC AGCGCGACTG GGATGTGAAC GCCGCAGCCG TTCGTGCTCT GCCTGTTCTG
     2041 GAGAAAATCC AGAAAGAGTC TGGTAAAGCC TCGCTGGCGG ATATCATAGT GCTGGCTGGT
     2101 GTGGTTGGTG TTGAGAAAGC CGCAAGCGCC GCAGGTTTGA GCATTCATGT ACCGTTTGCG
     2161 CCGGGTCGCG TTGATGCGCG TCAGGATCAG ACTGACATTG AGATGTTTGA GCTGCTGGAG
     2221 CCAATTGCTG ACGGTTTCCG TAACTATCGC GCTCGTCTGG ACGTTTCCAC CACCGAGTCA
     2281 CTGCTGATCG ACAAAGCACA GCAACTGACG CTGACCGCGC CGGAAATGAC TGCGCTGGTG
     2341 GGCGGCATGC GTGTACTGGG TGCCAACTTC GATGGCAGCA AAAACGGCGT CTTCACTGAC
     2401 CGCGTTGGCG TATTGAGCAA TGACTTCTTC GTGAACTTGC TGGATATGCG TTACGAGTGG
     2461 AAAGCGACCG ACGAATCGAA AGAGCTGTTC GAAGGCCGTG ACCGTGAAAC CGGCGAAGTG
     2521 AAATTTACGG CCAGCCGTGC GGATCTGGTG TTTGGTTCTA ACTCCGTCCT GCGTGCGGTG
     2581 GCGGAAGTTT ACGCCAGTAG CGATGCCCAC GAGAAGTTTG TTAAAGACTT CGTGGCGGCA
     2641 TGGGTGAAAG TGATGAACCT CGACCGTTTC GACCTGCTGT aaccgttcat ttatcacaaa
     2701 aggattgttc gatGaaatca atcgctgtca tcggcggtgg catcacgggt gtcaccacgg
     2761 cctacgcgct ggcccggcgc ggattctccg tcacgctgtt cgaacggcac cgctatgccg
     2821 ccatggagac ctcgttcgcc aacggcggcc agttgtcggc ctccaacgcc gaggtctgga
     2881 cccactggtc gaccctcgtc aagggcatga aatggatgct gcgcaacgac gccccgttgt
     2941 tggtcaatcc ccggcccagc tggcacaagc tgtcctggtt cgccgagttc atcgccgcga
     3001 tcccccacta ccgccgcaac accatcgaga ccgcgcgcct ggccatcgcg gcgcgtgagc
     3061 acctgttcgc ctgggcggcg caggaaggca tcgatttcga cctgaagaag gaaggcatcc
     3121 tccatatcta ccgcgacaag accggcttcg agcatgccgg ccgggtctcg gcactgctgg
     3181 cgcagggcgg gctggaacgg cgcgccgtga cgcccgagga gatgcgcgcg atcgagccga
     3241 cgctggccgg ctcgtactac ggcggctact tcaccgagag cgattccacc ggcgacatcc
     3301 acaagttcac cagcggcctg gccgcagcca tcgatcggct gggcgtgcgc tgcctgtacg
     3361 gccaggacgt gcgggcggtg aagaccgacg gcaggcgcgc caccgtgacc gtgcaggacg
     3421 gcgccgacac ggccacctcc accttcgacg gcgccgtgat ctgcgccggc accgccagcc
     3481 gcgcactggc cgccgggctg ggcgaccgcg tcaacatcta tccggtcaag ggctattcga
     3541 tcacggtcaa cctgaccgat gaggccagcc gcgccgccgc accggtggtc agcctgctcg
     3601 acgacgaaac caagctggtc accagccgcc tgggcgacga ccgcttccgc gtggccggca
     3661 ccgccgagtt caacggctac aaccgcgata tccgcgccga ccgcatccgc ccgctggtgg
     3721 aatgggtgca gcagtgcttc cccggcgtca gcacgcgcag cgtggtgcct tgggccgggc
     3781 tgcggccgat gatgccggat atgctgccgc gcgtggggcg cgggcgtgcg gcctgcatct
     3841 tctacaacac cgggcatggg cacctggggt ggactttgtc ggcggtgacg gcggatatgg
     3901 tggcaggggt tgtcgaccag catgtcggcg cgaaggtggc agtcgcggcg ggtgcgccga
     3961 tggcattggc caaggctTga tgggctgaag ggtttaaGCG AAGACGAACA ATAAGGCCTC
     4021 CCAAATCGGG GGGCCTTTTT TATTGATAAC AAAAAGGCAA CACTATGACA TCGGAAAACC
     4081 CGTTACTGGC GCTGCGAGAG AAAATCAGCG CGCTGGATGA AAAATTATTA GCATTACTGG
     4141 CAGAGCGGCG CGAATtcaaa cagaaaggcc atggGtcgac ctgcagaagc ttagatctat
     4201 taccctgtta tccctactcg agttcatgtg cagctccatc agcaaaaggg gatgataagt
     4261 ttatcaccac cgactatttg caacagtgcc gttgatcgtg ctatgatcga ctgatgtcat
     4321 cagcggtgga gtgcaatgtc atgagggaag cggtgatcgc cgaagtatcg actcaactat
     4381 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     4441 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     4501 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     4561 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     4621 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     4681 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     4741 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     4801 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     4861 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     4921 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     4981 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     5041 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     5101 aaggcgagat caccaaggta gtcggcaaat aagatgccgc tcgccagtcg attggctgag
     5161 ctcatgaagt tcctattccg aagttccgcg aacgcgtaaa ggatctaggt gaagatcctt
     5221 tttgataatc tcatgaccaa aatcccttaa cgTtcacaca acatagccac ggGagttttc
     5281 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5341 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5401 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     5461 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5521 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     5581 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     5641 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     5701 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     5761 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     5821 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     5881 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
//