LOCUS       AP1_T7p_RBS1_AfAlaDH_Hi 4976 bp ds-DNA     circular     20-OCT-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    117..137
                     /label="N23"
     misc_feature    380..395
                     /label="F1\extra"
     misc_feature    488..502
                     /label="ter\of\lacI\F2"
     CDS             complement(601..1683)
                     /label="lacI"
     primer          1764..1783
                     /label="ACYCDuetUP1\Primer\#71178-3"
     promoter        1804..1828
                     /label="T7p"
     misc_feature    1826..1848
                     /label="LacO"
     misc_feature    1855..1897
                     /label="RBS1"
     misc_feature    1898..2860
                     /label="AfAlaDH (E. coli codon optimized)"
     CDS             1898..2860
                     /label="Translation 1898-2860"
                     /translation="ETLILTQEEVESLISMDEAMNAVEEAFRLYALGKAQMPPKVYLEFEKGDLRAMPAHLMGYAGLKWVNSHPGNPDKGLPTVMALMILNSPETGFPLAVMDATYTTSLRTGAAGGIAAKYLARKNSSVFGFIGCGTQAYFQLEALRRVFDIGEVKAYDVREKAAKKFVSYCEDRGISASVQPAEEASRCDVLVTTTPSRKPVVKAEWVEEGTHINAIGADGPGKQELDVEILKKAKIVVDDLEQAKHGGEINVAVSKGVIGVEDVHATIGEVIAGLKDGRESDEEITIFDSTGLAIQDVAVAKVVYENALSKNVGSKIKFFRI"
     misc_feature    2861..2885
                     /label="histagstop"
     CDS             2861..2885
                     /label="Translation 2861-2885"
                     /translation="SHHHHHH*"
     misc_feature    2864..2879
                     /label="SE"
     terminator      2889..3016
                     /label="T7\terminator"
     misc_feature    3086..3106
                     /label="N21"
     misc_feature    3255..3274
                     /label="Inner RBS1"
     CDS             3272..4064
                     /label="aadA"
     CDS             3272..4064
                     /label="Translation 3272-4064"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK*"
     misc_feature    4061..4079
                     /label="Inner N31"
     misc_feature    4184..4204
                     /label="N22"
     CDS             4257..4845
                     /label="pMB1"
ORIGIN
        1 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
       61 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatgct ggatccTtca
      121 aacagaaagg ccatggGgaa actacccata atacaagaaa agcccgtcac gggcttctca
      181 gggcgtttta tggcgggtct gctatgtggt gctatctgac tttttgctgt tcagcagttc
      241 ctgccctctg attttccagt ctgaccactt cggattatcc cgtgacaggt cattcagact
      301 ggctaatgca cccagtaagg cagcggtatc atcaacaggc ttacccgtct tactgtcggg
      361 aattcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcctct agatttcagt
      421 gcaatttatc tcttcaaatg tagcacctga agtcagcccc atacgatata agttgtaatt
      481 ctcatgttag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag
      541 ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc
      601 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
      661 cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga
      721 gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc
      781 cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata
      841 acatgagctg tcttcggtat cgtcgtatcc cactaccgag atgtccgcac caacgcgcag
      901 cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat
      961 cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc
     1021 actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg
     1081 ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat
     1141 ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga
     1201 gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt
     1261 agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag
     1321 cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct
     1381 tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc
     1441 cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa
     1501 cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat
     1561 cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg
     1621 ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt
     1681 cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt
     1741 tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta
     1801 ggaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa
     1861 taattttgtt taactttaag aaggagatat acatatgGAA ACATTAATCC TGACTCAGGA
     1921 GGAAGTTGAA AGCCTTATCT CTATGGATGA AGCGATGAAT GCCGTGGAGG AAGCATTTCG
     1981 TTTGTACGCG TTAGGCAAAG CACAAATGCC TCCAAAGGTT TACCTGGAAT TTGAGAAAGG
     2041 TGATCTTCGC GCGATGCCCG CGCACCTTAT GGGTTACGCC GGTCTGAAGT GGGTCAATAG
     2101 TCATCCGGGA AATCCTGATA AGGGGCTGCC CACGGTTATG GCGCTGATGA TCCTGAACAG
     2161 CCCGGAGACT GGCTTCCCGT TGGCTGTAAT GGACGCAACT TACACCACGT CGTTACGTAC
     2221 TGGCGCGGCA GGCGGCATTG CCGCGAAATA TCTTGCCCGC AAGAACTCGA GTGTCTTCGG
     2281 TTTCATTGGC TGTGGGACTC AAGCCTATTT CCAATTAGAA GCTCTTCGTC GCGTTTTCGA
     2341 CATCGGTGAG GTCAAGGCAT ATGACGTTCG TGAGAAGGCC GCCAAGAAAT TCGTGTCGTA
     2401 CTGCGAAGAT CGCGGGATTT CCGCCTCCGT CCAGCCGGCT GAAGAGGCCT CACGTTGTGA
     2461 TGTTCTTGTG ACGACCACAC CGAGCCGTAA GCCCGTGGTG AAAGCAGAGT GGGTGGAGGA
     2521 AGGAACCCAC ATCAATGCAA TCGGTGCCGA TGGACCTGGT AAGCAGGAAC TTGACGTTGA
     2581 GATTCTTAAA AAAGCAAAAA TCGTCGTTGA TGATCTGGAG CAAGCAAAAC ATGGTGGGGA
     2641 AATCAATGTC GCAGTGAGTA AGGGCGTGAT TGGAGTCGAG GACGTCCACG CAACAATTGG
     2701 AGAGGTTATC GCCGGCTTAA AAGACGGTCG TGAAAGTGAC GAGGAGATCA CGATCTTCGA
     2761 TTCGACTGGA CTGGCAATCC AGGATGTAGC TGTCGCGAAA GTGGTCTACG AGAACGCATT
     2821 GTCGAAGAAT GTGGGGAGCA AGATTAAGTT CTTTCGTATT Tctcatcatc atcaccatca
     2881 ctgaGggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
     2941 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga
     3001 ggaactatat ccggatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc
     3061 ctacagcatc cagggtgacg gtgccTTccc tcgactcaca cttgGGtcga cctgcagaag
     3121 cttagatcta ttaccctgtt atccctactc gagttcatgt gcagctccat cagcaaaagg
     3181 ggatgataag tttatcacca ccgactattt gcaacagtgc cgttgatcgt gctatgatcg
     3241 actgatgtca tcagcggtgg agtgcaatgt catgagggaa gcggtgatcg ccgaagtatc
     3301 gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc
     3361 cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt
     3421 gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct
     3481 tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat
     3541 tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg
     3601 agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga
     3661 tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc
     3721 ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt
     3781 aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt
     3841 gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga
     3901 ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc
     3961 ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt
     4021 ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taagatgccg ctcgccagtc
     4081 gattggctga gctcatgaag ttcctattcc gaagttccgc gaacgcgtaa aggatctagg
     4141 tgaagatcct ttttgataat ctcatgacca aaatccctta acgTtcacac aacatagcca
     4201 cggGagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     4261 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     4321 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     4381 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     4441 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     4501 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     4561 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     4621 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     4681 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     4741 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     4801 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     4861 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     4921 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcg
//