LOCUS       pLSU-1//t35S[TMV]:MCS:N 5205 bp ds-DNA     circular     16-AUG-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      1..195
                     /label="VS1 ORI"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          226..243
                     /label="pLSU-1 5' F"
                     /note="sequence: gcaaaaagcgcctaccct"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    362..385
                     /label="T-DNA LB Repeat"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          388..415
                     /label="315bp t35S F"
                     /note="sequence: ATATTGTCGCATGCGGAAACCTCCTCGGATTC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          390..415
                     /label="t35S SphI F"
                     /note="sequence: ATATTGTCGCATGCGGAAACCTCCTCGGATTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          398..415
                     /label="HindIII t35s fwd"
                     /note="sequence: GGAAGAGGAAGCTTGGAAACCTCCTCGGATTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    398..712
                     /label="315bp t35S"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          559..578
                     /label="LBS41 (35S_SF1)"
                     /note="sequence: GAGGAGCATCGTGGAAAAAG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          597..624
                     /label="35sF"
                     /note="sequence: CTTCAAAGCAAGTGGATTGATGTGATAT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          642..662
                     /label="Kanamycin Sequence Primer Reverse"
                     /note="sequence: ATGACGCACAATCCCACTATC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          683..736
                     /label="Ovr-t35s-UTR-F"
                     /note="sequence: ATATAAGGAAGTTCATTTCATTTGGAGAGGACACGCTCGAGTATAAGAGCTCAT"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(690..718)
                     /label="315bp t35S R"
                     /note="sequence: GACAATATCTCGAGCCTCTCCAAATGAAATGAACTTC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(692..712)
                     /label="t35s_R"
                     /note="sequence: CCTCTCCAAATGAAATGAACT"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(693..733)
                     /label="pLSU-1//t35s:MCS:NosT___Linear REV"
                     /note="sequence: GCTCTTATACTCGAGCGTGTGCCTCTCCAAATGAAATGAAC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    713..805
                     /label="TMV 5' UTR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="TMV Leader Sequence https://doi.org/10.1016/j.bbrc.2010.11.102 "
     primer          complement(775..804)
                     /label="LBS17 (35S 5' Rev Bracket)"
                     /note="sequence: GTATCGATAATTGTAAATGTAATTGTAATG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(775..805)
                     /label="TMV_MluI_R"
                     /note="sequence: GAGCACGCGTGTATCGATAATTGTAAATGTAATTGTAATG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(775..826)
                     /label="Ovr-t35s-UTR-R"
                     /note="sequence: ATCCGAATTCGGTACCCTCGATGTATCGATAATTGTAAATGTAATTGTAATG"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          806..827
                     /label="MluI_MCS_AtPsaK_F"
                     /note="sequence: TTTGTAGAATCAACTAAGAAACGCGTGCTCGAGGGTACCGAATTCGGATC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(806..827)
                     /label="MCS_BstZ17I_R"
                     /note="sequence: tgacCTGCAGGTATACGATCCGAATTCGGTACCCTCGA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          828..857
                     /label="NosT F"
                     /note="sequence: ATATTGTCCTGCAGGATCGTTCAAACATTTGGCAATA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          834..853
                     /label="ffLUZ-NosT-f"
                     /note="sequence: GGGGGAAGAGTAAGTTGTAACCCGGGGATCGTTCAAACATTTGGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     regulatory      835..1090
                     /label="NOS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    836..1087
                     /label="Nos Terminator"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1011..1031)
                     /label="NosT-r_br"
                     /note="sequence: attttgttttctatcgcgtat"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(1065..1096)
                     /label="NosT R"
                     /note="sequence: GACAATATGAGCTCCCCGATCTAGTAACATAGATGACACC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1097..1158
                     /label="T-DNA RB Repeat + Overdrive"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1164..1273
                     /label="Tn903 nptI Promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(1239..1264)
                     /label="pLSU1_3R"
                     /note="sequence: TGTATTACTGTTTATGTAAGCAGACA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(1239..1264)
                     /label="pLSU-1 3' R"
                     /note="sequence: tgtattactgtttatgtaagcagaca"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(1239..1264)
                     /label="pLSU 3R"
                     /note="sequence: TGTATTACTGTTTATGTAAGCAGACA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(1239..1264)
                     /label="pLSU1_3'_R"
                     /note="sequence: TGTATTACTGTTTATGTAAGCAGACA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(1239..1264)
                     /label="pLSU-1 3' Rev"
                     /note="sequence: tgtattactgtttatgtaagcagaca"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1249..1273)
                     /label="pNPTI R"
                     /note="sequence: AACACCCCTTGTATTACTGTTTATG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(1252..1273)
                     /label="Seq8_R"
                     /note="sequence: AACACCCCTTGTATTACTGTTT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          1261..1280
                     /label="C-1F"
                     /note="sequence: TACAAGGGGTGTTATGAGCC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          1274..1294
                     /label="NPT1"
                     /note="sequence: ATGAGCCATATTCAACGGGAA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     CDS             1274..2089
                     /label="Tn903 nptI CDS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(1281..1300)
                     /label="BP NptI R"
                     /note="sequence: AGACGTTTCCCGTTGAATAT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    2090..2175
                     /label="Tn903 nptI Terminator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     rep_origin      2207..2763
                     /label="ColE1 ORI"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_binding    2836..2858
                     /label="misc_binding"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             3009..3638
                     /label="staA CDS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     gene            3965..5140
                     /label="repA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
ORIGIN
        1 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
       61 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
      121 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
      181 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
      241 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
      301 ccgctcaaaa atggctggcc tacggccagg caatctgcta gcgcgcggac aagctaggat
      361 tggcaggata tattcagttg taaatGATAT CGCATGCGGA AACCTCCTCG GATTCcattg
      421 cccagctatc tgtcacttta ttgagaagat agtggaaaag gaaggtggct cctacaaatg
      481 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
      541 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      601 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
      661 tccttcgcaa gacccttcct ctatataagG AAGTTCATTT CATTTGGAGA GGACACGCTC
      721 GAGTATAAGA GCTCATTTTT ACAACAATTA CCAACAACAA CAAACAACAA ACAACATTAC
      781 AATTACATTT ACAATTATCG ATACATCGAG GGTACCGAAT TCGGATCCCT GCAGGATCGT
      841 TCAAACATTT GGCAATAaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
      901 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
      961 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     1021 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcGGTGTC ATCTATGTTA
     1081 CTAGATCGGG Gagctctggc aggatatatg cggttgtaat tcatttttat tgtctaaatt
     1141 tctgtatttg tttgtttgca attagatcca cgttgtgtct caaaatctct gatgttacat
     1201 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     1261 tacaaggggt gttatgagcc atattcaacg ggaaacgtct tgttcgaggc cgcgattaaa
     1321 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     1381 aggtgcgaca atctaccgat tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     1441 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     1501 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     1561 actcaccact gcgatcccag ggaaaacagc attccaggta ttagaagaat atcctgattc
     1621 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     1681 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
     1741 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     1801 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca
     1861 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     1921 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     1981 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     2041 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt
     2101 taattggttg taacactggc agagcattac gctgacttga cgggacggcg gctttgttga
     2161 atcaattgat cttcttgaaa tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2221 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2281 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     2341 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2401 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2461 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2521 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2581 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2641 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2701 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2761 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2821 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     2881 tctgatcctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
     2941 agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag
     3001 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
     3061 tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
     3121 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
     3181 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
     3241 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag
     3301 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc
     3361 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg
     3421 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg
     3481 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg
     3541 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc
     3601 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat
     3661 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc
     3721 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc
     3781 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct
     3841 gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat
     3901 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg
     3961 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct
     4021 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa
     4081 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa
     4141 ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg
     4201 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc
     4261 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta
     4321 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa
     4381 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt
     4441 ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt
     4501 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg
     4561 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa
     4621 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca
     4681 gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat
     4741 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct
     4801 agttgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca
     4861 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg
     4921 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag
     4981 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct
     5041 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta
     5101 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg
     5161 gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcc
//