LOCUS       pLSU-1/o35S:mNG:NosT    6337 bp ds-DNA     circular     16-AUG-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      1..195
                     /label="VS1 ORI"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    362..385
                     /label="T-DNA LB Repeat"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    398..1232
                     /label="CaMV o35s "
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1197..1203
                     /label="tata"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1238..1948
                     /label="mNeonGreen"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     regulatory      1967..2222
                     /label="NOS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1968..2219
                     /label="Nos Terminator"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(2143..2163)
                     /label="NosT-r_br"
                     /note="sequence: attttgttttctatcgcgtat"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    2229..2290
                     /label="T-DNA RB Repeat + Overdrive"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2296..2405
                     /label="Tn903 nptI Promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             2406..3221
                     /label="Tn903 nptI CDS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3222..3307
                     /label="Tn903 nptI Terminator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     rep_origin      3339..3895
                     /label="ColE1 ORI"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_binding    3968..3990
                     /label="misc_binding"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             4141..4770
                     /label="staA CDS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     gene            5097..6272
                     /label="repA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
ORIGIN
        1 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
       61 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
      121 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
      181 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
      241 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
      301 ccgctcaaaa atggctggcc tacggccagg caatctgcta gcgcgcggac aagctaggat
      361 tggcaggata tattcagttg taaatGATAT CGCATGCaga ttagcctttt caatttcaga
      421 aagaatgcta acccacagat ggttagagag gcttacgcag caggtctcat caagacgatc
      481 tacccgagca ataatctcca ggaaatcaaa taccttccca agaaggttaa agatgcagtc
      541 aaaagattca ggactaactg catcaagaac acagagaaag atatatttct caagatcaga
      601 agtactattc cagtatggac gattcaaggc ttgcttcaca aaccaaggca agtaatagag
      661 attggagtct ctaaaaaggt agttcccact gaatcaaagg ccatggagtc aaagattcaa
      721 atagaggacc taacagaact cgccgtaaag actggcgaac agttcataca gagtctctta
      781 cgactcaatg acaagaagaa aatcttcgtc aacatggtgg agcacgacac acttgtctac
      841 tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa
      901 agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg
      961 aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc
     1021 atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
     1081 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc
     1141 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata
     1201 taaggaagtt catttcattt ggagagaaca cgTCGAGATG GTTTCCAAAG GTGAGGAAGA
     1261 TAATATGGCT AGTTTACCTG CCACACATGA GCTACACATA TTCGGCAGCA TAAACGGTGT
     1321 AGATTTTGAT ATGGTTGGAC AAGGGACAGG TAATCCTAAT GATGGATATG AGGAGTTGAA
     1381 CCTCAAATCT ACCAAGGGGG ACCTTCAATT CTCCCCATGG ATCCTAGTCC CTCACATTGG
     1441 ATACGGCTTC CACCAGTACT TACCATACCC AGATGGAATG TCCCCTTTCC AAGCAGCCAT
     1501 GGTTGACGGT TCCGGGTACC AGGTACATAG GACAATGCAA TTTGAGGACG GGGCTTCTCT
     1561 GACTGTGAAT TACCGATATA CCTATGAAGG CAGTCACATA AAGGGCGAAG CACAAGTGAA
     1621 GGGCACCGGA TTTCCTGCCG ACGGGCCTGT AATGACTAAT AGCTTGACCG CCGCAGATTG
     1681 GTGCAGGTCA AAAAAGACCT ACCCAAATGA CAAGACAATA ATCTCTACAT TTAAGTGGAG
     1741 CTACACTACA GGGAATGGTA AACGATATAG ATCAACTGCT AGAACCACAT ATACCTTCGC
     1801 TAAACCTATG GCAGCCAATT ATCTGAAGAA CCAGCCCATG TATGTGTTCC GAAAGACTGA
     1861 ACTCAAGCAT AGCAAAACCG AACTCAACTT CAAAGAGTGG CAAAAGGCTT TCACTGACGT
     1921 GATGGGAATG GACGAGTTAT ATAAGTAAGA ATTCGGATCC CTGCAGGATC GTTCAAACAT
     1981 TTGGCAATAa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
     2041 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
     2101 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
     2161 aatatagcgc gcaaactagg ataaattatc gcgcgcGGTG TCATCTATGT TACTAGATCG
     2221 GGGagctctg gcaggatata tgcggttgta attcattttt attgtctaaa tttctgtatt
     2281 tgtttgtttg caattagatc cacgttgtgt ctcaaaatct ctgatgttac attgcacaag
     2341 ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg
     2401 gtgttatgag ccatattcaa cgggaaacgt cttgttcgag gccgcgatta aattccaaca
     2461 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
     2521 caatctaccg attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
     2581 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta
     2641 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
     2701 ctgcgatccc agggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
     2761 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
     2821 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
     2881 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
     2941 ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt
     3001 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
     3061 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
     3121 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
     3181 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt
     3241 tgtaacactg gcagagcatt acgctgactt gacgggacgg cggctttgtt gaatcaattg
     3301 atcttcttga aatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     3361 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     3421 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     3481 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3541 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3601 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     3661 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     3721 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     3781 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     3841 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     3901 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     3961 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gatctgatcc
     4021 tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg
     4081 tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc
     4141 atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc
     4201 catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc
     4261 cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc
     4321 atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg
     4381 atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc
     4441 gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag
     4501 ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg
     4561 cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag
     4621 ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc
     4681 ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg
     4741 gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa
     4801 gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg
     4861 ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc
     4921 acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg
     4981 aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta
     5041 gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc
     5101 cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgt ctgccggccc
     5161 tgcaatggca ctggaacccc caagcccgag gaatcggcgt gagcggtcgc aaaccatccg
     5221 gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc
     5281 aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg
     5341 ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta
     5401 ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg
     5461 gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc
     5521 gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag
     5581 ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc
     5641 taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc
     5701 gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag
     5761 acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga
     5821 agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct
     5881 acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagttgatt
     5941 ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt
     6001 actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag
     6061 gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag
     6121 agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt
     6181 acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc
     6241 tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg
     6301 ccctagcagg ggaaaaaggt cgaaaaggtc tctttcc
//