LOCUS       pLSU-1;_o35S:MCS:NosT   5633 bp ds-DNA     circular     16-AUG-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      1..195
                     /label="VS1 ORI"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          226..243
                     /label="pLSU-1 5' F"
                     /note="sequence: gcaaaaagcgcctaccct"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          362..380
                     /label="TI LB Fwd"
                     /note="sequence: GGCAGGATATATTCAGTTG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    362..385
                     /label="T-DNA LB Repeat"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          388..419
                     /label="35S F"
                     /note="sequence: ATATTGTCGCATGCAGATTAGCCTTTTCAATTTCAG"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          397..420
                     /label="35sP Forward-BamHI"
                     /note="sequence: TTTCGGATCCAGATTAGCCTTTTCAATTTCAGA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          398..417
                     /label="CaMV-35S-1-20-H"
                     /note="sequence: AGATTAGCCTTTTCAATTTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          398..419
                     /label="ToMCP- F"
                     /note="sequence: AGATTAGCCTTTTCAATTTCAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     regulatory      398..1232
                     /label="CaMV 35S"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     1023..1042
                     /label="Binding Region"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          1074..1093
                     /label="LBS41 (35S_SF1)"
                     /note="sequence: GAGGAGCATCGTGGAAAAAG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(1214..1238)
                     /label="35SR"
                     /note="sequence: GACAATATCTCGAGCGTGTTCTCTCCAAATGAA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          1256..1285
                     /label="NosT F"
                     /note="sequence: ATATTGTCCTGCAGGATCGTTCAAACATTTGGCAATA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     regulatory      1263..1518
                     /label="NOS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1264..1515
                     /label="Nos Terminator"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1493..1524)
                     /label="NosT R"
                     /note="sequence: GACAATATGAGCTCCCCGATCTAGTAACATAGATGACACC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1525..1586
                     /label="T-DNA RB Repeat + Overdrive"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1592..1701
                     /label="Tn903 nptI Promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(1667..1692)
                     /label="pLSU-1 3' R"
                     /note="sequence: tgtattactgtttatgtaagcagaca"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             1702..2517
                     /label="Tn903 nptI CDS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2518..2603
                     /label="Tn903 nptI Terminator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     rep_origin      2635..3191
                     /label="ColE1 ORI"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_binding    3264..3286
                     /label="misc_binding"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             3437..4066
                     /label="staA CDS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     gene            4393..5568
                     /label="repA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
ORIGIN
        1 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
       61 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
      121 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
      181 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
      241 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
      301 ccgctcaaaa atggctggcc tacggccagg caatctgcta gcgcgcggac aagctaggat
      361 tggcaggata tattcagttg taaatGATAT CGCATGCAGA TTAGCCTTTT CAATTTCAGa
      421 aagaatgcta acccacagat ggttagagag gcttacgcag caggtctcat caagacgatc
      481 tacccgagca ataatctcca ggaaatcaaa taccttccca agaaggttaa agatgcagtc
      541 aaaagattca ggactaactg catcaagaac acagagaaag atatatttct caagatcaga
      601 agtactattc cagtatggac gattcaaggc ttgcttcaca aaccaaggca agtaatagag
      661 attggagtct ctaaaaaggt agttcccact gaatcaaagg ccatggagtc aaagattcaa
      721 atagaggacc taacagaact cgccgtaaag actggcgaac agttcataca gagtctctta
      781 cgactcaatg acaagaagaa aatcttcgtc aacatggtgg agcacgacac acttgtctac
      841 tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa
      901 agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg
      961 aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc
     1021 atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
     1081 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc
     1141 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata
     1201 taaggaagtt catTTCATTT GGAGAGAACA CGCTCGAGGG TACCGAATTC GGATCCCTGC
     1261 AGGATCGTTC AAACATTTGG CAATAaagtt tcttaagatt gaatcctgtt gccggtcttg
     1321 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
     1381 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
     1441 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcGGTGTCAT
     1501 CTATGTTACT AGATCGGGGa gctctggcag gatatatgcg gttgtaattc atttttattg
     1561 tctaaatttc tgtatttgtt tgtttgcaat tagatccacg ttgtgtctca aaatctctga
     1621 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
     1681 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcttg ttcgaggccg
     1741 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
     1801 gggcaatcag gtgcgacaat ctaccgattg tatgggaagc ccgatgcgcc agagttgttt
     1861 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
     1921 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
     1981 gcatggttac tcaccactgc gatcccaggg aaaacagcat tccaggtatt agaagaatat
     2041 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
     2101 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
     2161 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
     2221 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc
     2281 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
     2341 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     2401 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     2461 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca
     2521 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcggc
     2581 tttgttgaat caattgatct tcttgaaatc ctttttttct gcgcgtaatc tgctgcttgc
     2641 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     2701 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     2761 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     2821 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     2881 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     2941 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     3001 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     3061 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     3121 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     3181 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     3241 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     3301 ttgagtgatc tgatcctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg
     3361 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa
     3421 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa
     3481 gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt
     3541 agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc
     3601 gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg
     3661 gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat
     3721 caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac
     3781 cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc
     3841 ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc
     3901 gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc
     3961 aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg
     4021 cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa
     4081 gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc
     4141 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag
     4201 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt
     4261 accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat
     4321 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac
     4381 cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg
     4441 ggttgtctgc cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc
     4501 ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag
     4561 aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt
     4621 gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc
     4681 ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg
     4741 atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt
     4801 ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac
     4861 gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg
     4921 atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag
     4981 cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat
     5041 ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt
     5101 gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa
     5161 gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag
     5221 atcgagctag ttgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg
     5281 acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg
     5341 gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc
     5401 agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca
     5461 aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc
     5521 atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag
     5581 atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcc
//