LOCUS       pLSU-1/t35S:MCS:NosT    5113 bp ds-DNA     circular     16-AUG-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      1..195
                     /label="VS1 ORI"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          226..243
                     /label="pLSU-1 5' F"
                     /note="sequence: gcaaaaagcgcctaccct"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    362..385
                     /label="T-DNA LB Repeat"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          388..415
                     /label="315bp t35S F"
                     /note="sequence: ATATTGTCGCATGCGGAAACCTCCTCGGATTC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    398..713
                     /label="315bp t35S"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          457..480
                     /label="LBS30"
                     /note="sequence: AAAGGAAGGTGGCTCCTACAAATG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          559..578
                     /label="LBS41"
                     /note="sequence: GAGGAGCATCGTGGAAAAAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          597..624
                     /label="35sF"
                     /note="sequence: CTTCAAAGCAAGTGGATTGATGTGATAT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(690..718)
                     /label="315bp t35S R"
                     /note="sequence: GACAATATCTCGAGCCTCTCCAAATGAAATGAACTTC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          736..765
                     /label="NosT F"
                     /note="sequence: ATATTGTCCTGCAGGATCGTTCAAACATTTGGCAATA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          743..766
                     /label="fusion SSU1/nosT fwd"
                     /note="sequence: GATCGTTCAAACATTTGGCAATAa"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     regulatory      743..998
                     /label="NOS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    744..995
                     /label="Nos Terminator"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(884..908)
                     /label="nosTR"
                     /note="sequence: ATTGCGGGACTCTAATCATAAAAAC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(919..939)
                     /label="NosT-r_br"
                     /note="sequence: attttgttttctatcgcgtat"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(972..995)
                     /label="NosT R"
                     /note="sequence: GATCTAGTAACATAGATGACACCg"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(972..995)
                     /label="mNG(nosT) reverse sacI"
                     /note="sequence: GATCTAGTAACATAGATGACACCg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(973..1004)
                     /label="NosT R"
                     /note="sequence: GACAATATGAGCTCCCCGATCTAGTAACATAGATGACACC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(978..1005)
                     /label="pLSU-1 NosT R"
                     /note="sequence: GACAATATagagctCCCCGATCTAGTAACATAGATG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(978..1005)
                     /label="NosT-r"
                     /note="sequence: AAAGAGCTCCCCGATCTAGTAACATAGATG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    1005..1066
                     /label="T-DNA RB Repeat + Overdrive"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1072..1181
                     /label="Tn903 nptI Promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(1147..1172)
                     /label="pLSU-1 3' R"
                     /note="sequence: tgtattactgtttatgtaagcagaca"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             1182..1997
                     /label="Tn903 nptI CDS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1998..2083
                     /label="Tn903 nptI Terminator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     rep_origin      2115..2671
                     /label="ColE1 ORI"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_binding    2744..2766
                     /label="misc_binding"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             2917..3546
                     /label="staA CDS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     gene            3873..5048
                     /label="repA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
ORIGIN
        1 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
       61 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
      121 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
      181 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
      241 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
      301 ccgctcaaaa atggctggcc tacggccagg caatctgcta gcgcgcggac aagctaggat
      361 tggcaggata tattcagttg taaatGATAT CGCATGCGGA AACCTCCTCG GATTCcattg
      421 cccagctatc tgtcacttta ttgagaagat agtggaaaag gaaggtggct cctacaaatg
      481 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
      541 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      601 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
      661 tccttcgcaa gacccttcct ctatataagG AAGTTCATTT CATTTGGAGA GGCTCGAGGG
      721 TACCGAATTC GGATCCCTGC AGGATCGTTC AAACATTTGG CAATAaagtt tcttaagatt
      781 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
      841 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
      901 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
      961 attatcgcgc gcGGTGTCAT CTATGTTACT AGATCGGGGa gctctggcag gatatatgcg
     1021 gttgtaattc atttttattg tctaaatttc tgtatttgtt tgtttgcaat tagatccacg
     1081 ttgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa
     1141 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg
     1201 aaacgtcttg ttcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata
     1261 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctaccgattg tatgggaagc
     1321 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag
     1381 atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt
     1441 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatcccaggg aaaacagcat
     1501 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt
     1561 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat
     1621 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg
     1681 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc
     1741 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg
     1801 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc
     1861 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc
     1921 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc
     1981 tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag agcattacgc
     2041 tgacttgacg ggacggcggc tttgttgaat caattgatct tcttgaaatc ctttttttct
     2101 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     2161 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     2221 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     2281 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     2341 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     2401 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     2461 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     2521 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     2581 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     2641 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     2701 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     2761 ggataaccgt attaccgcct ttgagtgatc tgatcctgaa gaaaccgagc gccgccgtct
     2821 aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat
     2881 gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac
     2941 cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc
     3001 gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg
     3061 gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc
     3121 gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg
     3181 gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc
     3241 ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc
     3301 acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc
     3361 ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc
     3421 acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa
     3481 cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc
     3541 atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc
     3601 gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca
     3661 tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg
     3721 tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt
     3781 aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa
     3841 aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt
     3901 tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg aacccccaag
     3961 cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc
     4021 tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg
     4081 aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat
     4141 cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc
     4201 aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca
     4261 tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct
     4321 acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt
     4381 gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc
     4441 gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca
     4501 agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc
     4561 ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg
     4621 tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg
     4681 ggcggccgga gtacatcgag atcgagctag ttgattggat gtaccgcgag atcacagaag
     4741 gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg
     4801 gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt
     4861 tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg
     4921 tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc
     4981 aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg
     5041 gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa
     5101 aaggtctctt tcc
//