LOCUS       Copy_of_pLSU-1/t35S:mNG 6007 bp ds-DNA     circular     16-AUG-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      1..195
                     /label="VS1 ORI"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    362..385
                     /label="T-DNA LB Repeat"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    398..713
                     /label="315bp t35S"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          559..578
                     /label="LBS41 (35S_SF1)"
                     /note="sequence: GAGGAGCATCGTGGAAAAAG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    719..916
                     /label="mNeonGreen - Tomato Codon Opt."
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          757..776
                     /label="MnG-qPCR-F1"
                     /note="sequence: ACCTGCCACACATGAGCTAC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(897..916)
                     /label="mNG07 (mNG 5' Rev Bracket)"
                     /note="sequence: GTGAGGGACTAGGATCCATG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     intron          917..1105
                     /label="intron"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          1106..1125
                     /label="mNG08 (mNG 3' Fwd Bracket)"
                     /note="sequence: ATTGGATACGGCTTCCACCA"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    1106..1618
                     /label="mNeonGreen - Tomato Codon Opt."
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    1112..1114
                     /label="Y69 (Key Chromophore Residue)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(1270..1289)
                     /label="MnG-qPCR-R1"
                     /note="sequence: TCACTTGTGCTTCGCCCTTT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     regulatory      1637..1892
                     /label="NOS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1638..1889
                     /label="Nos Terminator"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1899..1960
                     /label="T-DNA RB Repeat + Overdrive"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1966..2075
                     /label="Tn903 nptI Promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             2076..2891
                     /label="Tn903 nptI CDS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2892..2977
                     /label="Tn903 nptI Terminator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     rep_origin      3009..3565
                     /label="ColE1 ORI"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_binding    3638..3660
                     /label="misc_binding"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             3811..4440
                     /label="staA CDS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     gene            4767..5942
                     /label="repA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
ORIGIN
        1 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
       61 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
      121 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
      181 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
      241 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
      301 ccgctcaaaa atggctggcc tacggccagg caatctgcta gcgcgcggac aagctaggat
      361 tggcaggata tattcagttg taaatGATAT CGCATGCGGA AACCTCCTCG GATTCcattg
      421 cccagctatc tgtcacttta ttgagaagat agtggaaaag gaaggtggct cctacaaatg
      481 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
      541 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      601 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
      661 tccttcgcaa gacccttcct ctatataagG AAGTTCATTT CATTTGGAGA GGCTCGAGAT
      721 GGTTTCCAAA GGTGAGGAAG ATAATATGGC TAGTTTACCT GCCACACATG AGCTACACAT
      781 ATTCGGCAGC ATAAACGGTG TAGATTTTGA TATGGTTGGA CAAGGGACAG GTAATCCTAA
      841 TGATGGATAT GAGGAGTTGA ACCTCAAATC TACCAAGGGG GACCTTCAAT TCTCCCCATG
      901 GATCCTAGTC CCTCACgtaa gtttctgctt ctacctttga tatatatata ataattatca
      961 ttaattagta gtaatataat atttcaaata tttttttcaa aataaaagaa tgtagtatat
     1021 agcaattgct tttctgtagt ttataagtgt gtatatttta atttataact tttctaatat
     1081 atgaccaaaa tttgttgatg tgcagATTGG ATACGGCTTC CACCAGTACT TACCATACCC
     1141 AGATGGAATG TCCCCTTTCC AAGCAGCCAT GGTTGACGGT TCCGGGTACC AGGTACATAG
     1201 GACAATGCAA TTTGAGGACG GGGCTTCTCT GACTGTGAAT TACCGATATA CCTATGAAGG
     1261 CAGTCACATA AAGGGCGAAG CACAAGTGAA GGGCACCGGA TTTCCTGCCG ACGGGCCTGT
     1321 AATGACTAAT AGCTTGACCG CCGCAGATTG GTGCAGGTCA AAAAAGACCT ACCCAAATGA
     1381 CAAGACAATA ATCTCTACAT TTAAGTGGAG CTACACTACA GGGAATGGTA AACGATATAG
     1441 ATCAACTGCT AGAACCACAT ATACCTTCGC TAAACCTATG GCAGCCAATT ATCTGAAGAA
     1501 CCAGCCCATG TATGTGTTCC GAAAGACTGA ACTCAAGCAT AGCAAAACCG AACTCAACTT
     1561 CAAAGAGTGG CAAAAGGCTT TCACTGACGT GATGGGAATG GACGAGTTAT ATAAGTAAGA
     1621 ATTCGGATCC CTGCAGGATC GTTCAAACAT TTGGCAATAa agtttcttaa gattgaatcc
     1681 tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat
     1741 aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca
     1801 attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc
     1861 gcgcgcGGTG TCATCTATGT TACTAGATCG GGGagctctg gcaggatata tgcggttgta
     1921 attcattttt attgtctaaa tttctgtatt tgtttgtttg caattagatc cacgttgtgt
     1981 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac
     2041 tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt
     2101 cttgttcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
     2161 ctcgcgataa tgtcgggcaa tcaggtgcga caatctaccg attgtatggg aagcccgatg
     2221 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
     2281 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
     2341 gtactcctga tgatgcatgg ttactcacca ctgcgatccc agggaaaaca gcattccagg
     2401 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
     2461 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc
     2521 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg
     2581 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct
     2641 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
     2701 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
     2761 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
     2821 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg
     2881 agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt
     2941 gacgggacgg cggctttgtt gaatcaattg atcttcttga aatccttttt ttctgcgcgt
     3001 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3061 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3121 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3181 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3241 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3301 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3361 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3421 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3481 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3541 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3601 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     3661 ccgtattacc gcctttgagt gatctgatcc tgaagaaacc gagcgccgcc gtctaaaaag
     3721 gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg
     3781 agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa
     3841 ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg
     3901 gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg
     3961 cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg
     4021 aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg
     4081 gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac
     4141 gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat
     4201 ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt
     4261 gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag
     4321 cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag
     4381 ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga
     4441 gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga
     4501 gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc
     4561 gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc
     4621 aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga
     4681 gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa
     4741 gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca
     4801 ggcgtaagcg gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag
     4861 gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg
     4921 atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag
     4981 aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc
     5041 aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag
     5101 attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg
     5161 tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc
     5221 ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt
     5281 acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag
     5341 ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct
     5401 gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa
     5461 acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg
     5521 tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc
     5581 cggagtacat cgagatcgag ctagttgatt ggatgtaccg cgagatcaca gaaggcaaga
     5641 acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt
     5701 ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga
     5761 cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca
     5821 agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg
     5881 gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct
     5941 aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc
     6001 tctttcc
//